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Previous issue date: 2016-02-16 / O carcinoma epiderm?ide de l?ngua oral (CELO) apresenta um comportamento biol?gico agressivo, com elevada propens?o ao desenvolvimento de met?stases nodais. Nesse contexto, a linfangiog?nese ? considerada um fen?meno importante para a dissemina??o das c?lulas tumorais e pode sofrer influ?ncia de est?mulos do microambiente. Os mast?citos t?m sido relacionados ? progress?o de neoplasias malignas, no entanto o seu papel na forma??o de vasos linf?ticos ainda n?o est? bem estabelecido. O prop?sito desta pesquisa foi avaliar poss?veis correla??es entre a densidade linf?tica, a contagem de mast?citos e o perfil clinicopatol?gico em casos de CELO, incluindo o estadiamento cl?nico TNM, a grada??o histol?gica de malignidade (Bryne, 1998) e a presen?a/aus?ncia de met?stases nodais. A amostra foi constitu?da por 50 casos de CELO, dos quais 26 apresentavam met?stase nodal, e os 24 restantes eram isentos de met?stases. A densidade linf?tica foi estabelecida como a m?dia de vasos linf?ticos imunomarcados pelo anticorpo anti-podoplanina (D2-40), identificados em cinco campos microsc?picos (200x). Para a an?lise dos mast?citos, foram quantificadas as c?lulas imunorreativas ao anticorpo anti-triptase, em cinco campos (400x). Destaca-se que ambas as imunomarca??es foram analisadas no centro tumoral e no front de invas?o. A densidade linf?tica intratumoral (DLI) foi superior nos casos em est?gios cl?nicos avan?ados (III-IV), quando comparados ?queles em est?gios iniciais (I-II), assim como nos casos metast?ticos em rela??o aos n?o-metast?ticos (p<0,05). N?o houve diferen?as estatisticamente significativas entre os casos de baixo grau e alto grau de malignidade no tocante ? DLI (p>0,05). De outro modo, a densidade linf?tica peritumoral (DLP) e as contagens de mast?citos n?o demonstraram rela??es significativas com nenhum dos par?metros clinicopatol?gicos avaliados (p>0,05). Tamb?m n?o foram encontradas correla??es significativas entre as densidades linf?ticas e as contagens de mast?citos, seja na regi?o intratumoral (r = -0,004; p=0,977) ou na peritumoral (r = -0,154; p=0,285). Os resultados do presente estudo sugerem que os vasos linf?ticos intratumorais contribuem na progress?o do CELO. Por sua vez, a DLP pode n?o ser suficiente para justificar diferen?as no comportamento biol?gico do CELO, o que sustenta a hip?tese de envolvimento de outros mecanismos na dissemina??o metast?tica das c?lulas malignas, que complementariam os efeitos da linfangiog?nese. Os mast?citos, ainda que realizem diversas fun??es pr?- e antitumorais, parecem n?o influenciar diretamente o potencial de agressividade do CELO. Adicionalmente, ? poss?vel que a quantidade destas c?lulas n?o seja um fator determinante para a forma??o de vasos linf?ticos. / Oral tongue squamous cell carcinoma (OTSCC) has an aggressive biological behavior, with a high propensity for the development of lymph node metastases. In this context, lymphangiogenesis is considered an important phenomenon for the spread of tumor cells and may be influenced by microenvironmental stimuli. Mast cells have been implicated in tumor progression, although their influence in the formation of lymphatic vessels is not well established. The aim of this study was to analyze, in a case series of OTSCC (n=50), possible correlations between lymphatic vessel density (LVD), mast cell count and clinicopathological features, including tumor-node-metastasis (TNM) stage, histological grade of malignancy (Bryne, 1998), and nodal metastasis. LVD was established as the mean number of lymphatic vessels immunostained by anti-podoplanin (D2-40) antibody, identified in five microscopic fields (200x). For the analysis of mast cells, tryptase-immunoreactive cells were quantified in five fields (400x). Both immunostainings were analyzed in the tumor center and invasion front. Intratumoral lymphatic density (ILD) was higher in cases in advanced clinical stages (III-IV), compared to those in initial stages (I-II), as well as in metastatic cases in respect of non-metastatic (p<0,05). There were no statistically significant differences between low-grade and high-grade malignancy cases with respect to ILD (p>0,05). Peritumoral lymphatic density (PLD) and mast cell counts showed no significant relations with any of the clinicopathological parameters evaluated (p>0,05). Also there were no significant correlations between LVD and mast cell counts, whether in intratumoral (r = -0,004; p=0,977) or peritumoral region (r = -0,154; p=0,285). The results of the present study suggest that intratumoral lymphatic vessels may contribute in part to the progression of OTSCC, although PLD may be insufficient to justify differences in biological behavior. This supports the hypothesis of involvement of other mechanisms in metastatic spread of malignant cells, which could complement the effects of lymphangiogenesis. Although mast cells perform several pro- and antitumoral functions, they do not appear to directly influence aggressiveness of OTSCC. In addition, the quantity of these cells may not be essential for lymphatic vessel formation.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20961 |
| Date | 16 February 2016 |
| Creators | Mafra, Rodrigo Porpino |
| Contributors | 01608789420, http://lattes.cnpq.br/7040457616034306, Carvalho, Cyntia Helena Pereira de, 01235589480, http://lattes.cnpq.br/3956936720129374, Silveira, Ericka Janine Dantas da, 02869049420, http://lattes.cnpq.br/2186658404241838, Pinto, Le?o Pereira |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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