The aims of this study were to identify Chloroviruses in two different Ontario freshwaters and to determine if the seasonal abundance patterns of Chloroviruses in different environments are similar. Gene fragments nearly identical to cultivated Chloroviruses were obtained from Lake Ontario and a nearby pond at the University of Toronto Mississauga (UTM) and novel Chlorovirus gene fragments were obtained from Lake Ontario. Quantification of these two Chlorovirus genes over several seasons revealed the presence of persistent viruses with different seasonal dynamics suggesting that different Chloroviruses replicate by infecting different hosts. Additionally, patterns of seasonal abundance and timings of peak abundances for individual viruses differed between Lake Ontario and the UTM pond, demonstrating the critical role of the environment in Chlorovirus dynamics. The observation of different Chloroviruses with different seasonal dynamics allows speculation that these viruses and their hosts stably coexist in Ontario freshwater environments.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/25910 |
| Date | 13 January 2011 |
| Creators | Rusanova, Oksana |
| Contributors | Short, Steven |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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