Global ETD Search

1	Seasonal Abundance of Different Chlorella Viruses in Two Contrasting Freshwater Environments in Ontario, Canada Rusanova, Oksana 13 January 2011 (has links) The aims of this study were to identify Chloroviruses in two different Ontario freshwaters and to determine if the seasonal abundance patterns of Chloroviruses in different environments are similar. Gene fragments nearly identical to cultivated Chloroviruses were obtained from Lake Ontario and a nearby pond at the University of Toronto Mississauga (UTM) and novel Chlorovirus gene fragments were obtained from Lake Ontario. Quantification of these two Chlorovirus genes over several seasons revealed the presence of persistent viruses with different seasonal dynamics suggesting that different Chloroviruses replicate by infecting different hosts. Additionally, patterns of seasonal abundance and timings of peak abundances for individual viruses differed between Lake Ontario and the UTM pond, demonstrating the critical role of the environment in Chlorovirus dynamics. The observation of different Chloroviruses with different seasonal dynamics allows speculation that these viruses and their hosts stably coexist in Ontario freshwater environments. Algal viruses Phycodnaviruses DNA polymerase Quantitative PCR freshwater Lake Ontario pond Chlorella viruses Chlorovirus abundance seasonal dynamics Chlorophyll a persistent 0329 0793 0307 0720
2	Diversity and Dynamics of Algal Viruses in the Bay of Quinte Rozon, Robin 17 July 2013 (has links) To initiate algal virus research in the Bay of Quinte, three stations were sampled biweekly throughout 2011. By targeting algal virus DNA polymerase, major capsid protein genes (MCP), and a Microcystis aeruginosa cyanophage (Ma-LMM01) tail sheath protein gene, PCR amplification revealed diverse and unique Phycodnaviruses (viruses of eukaryotic algae) and cyanophage. When analysed statistically, patterns of virus abundance suggested that the seasonality of any one virus cannot be generalised to predict that of other viruses, even among closely related viruses. This study also demonstrated a strong relationship between algal virus abundance and host biomass. It was found that despite the apparent heterogeneity of virus abundance across the Bay, virus abundance patterns clustered by sampling date and geographic location. By providing evidence for diverse algal viruses with complex seasonality, this work highlights significant gaps in the current understanding of Bay of Quinte phytoplankton ecology. Algal viruses Bay of Quinte Phycodnaviruses DNA polymerase major capsid protein gene Sheath protein gene Microcystis aeruginosa freshwater Lake Ontario Cyanophage abundance seasonality Algal bloom Quantitative PCR Chlorophyll a 0329
3	Seasonal Abundance of Different Chlorella Viruses in Two Contrasting Freshwater Environments in Ontario, Canada Rusanova, Oksana 13 January 2011 (has links) The aims of this study were to identify Chloroviruses in two different Ontario freshwaters and to determine if the seasonal abundance patterns of Chloroviruses in different environments are similar. Gene fragments nearly identical to cultivated Chloroviruses were obtained from Lake Ontario and a nearby pond at the University of Toronto Mississauga (UTM) and novel Chlorovirus gene fragments were obtained from Lake Ontario. Quantification of these two Chlorovirus genes over several seasons revealed the presence of persistent viruses with different seasonal dynamics suggesting that different Chloroviruses replicate by infecting different hosts. Additionally, patterns of seasonal abundance and timings of peak abundances for individual viruses differed between Lake Ontario and the UTM pond, demonstrating the critical role of the environment in Chlorovirus dynamics. The observation of different Chloroviruses with different seasonal dynamics allows speculation that these viruses and their hosts stably coexist in Ontario freshwater environments. Algal viruses Phycodnaviruses DNA polymerase Quantitative PCR freshwater Lake Ontario pond Chlorella viruses Chlorovirus abundance seasonal dynamics Chlorophyll a persistent 0329 0793 0307 0720
4	Diversity and Dynamics of Algal Viruses in the Bay of Quinte Rozon, Robin 17 July 2013 (has links) To initiate algal virus research in the Bay of Quinte, three stations were sampled biweekly throughout 2011. By targeting algal virus DNA polymerase, major capsid protein genes (MCP), and a Microcystis aeruginosa cyanophage (Ma-LMM01) tail sheath protein gene, PCR amplification revealed diverse and unique Phycodnaviruses (viruses of eukaryotic algae) and cyanophage. When analysed statistically, patterns of virus abundance suggested that the seasonality of any one virus cannot be generalised to predict that of other viruses, even among closely related viruses. This study also demonstrated a strong relationship between algal virus abundance and host biomass. It was found that despite the apparent heterogeneity of virus abundance across the Bay, virus abundance patterns clustered by sampling date and geographic location. By providing evidence for diverse algal viruses with complex seasonality, this work highlights significant gaps in the current understanding of Bay of Quinte phytoplankton ecology. Algal viruses Bay of Quinte Phycodnaviruses DNA polymerase major capsid protein gene Sheath protein gene Microcystis aeruginosa freshwater Lake Ontario Cyanophage abundance seasonality Algal bloom Quantitative PCR Chlorophyll a 0329

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