Since many approaches to dealing with algal blooms are inefficient, expensive, or harmful, it was concluded that a biologically-synthesized chemical agent, specifically inhibitory to pre-bloom algal cells, might prove helpful in controlling algal blooms. Fungi were chosen as the biological entities to investigate for such a chemical.

Reservoirs are the main drinking water sources in Taiwan. However, about half of 23 reservoirs suffer the eutrophication problems based on the calculated Carlson¡¦s TSI indexes for them. Thus, the reservoir water is seriously polluted. Chen-Ching Lake Reservoir, Fengshan Reservoir, and A-Kong-Tien Reservoir are three major reservoirs that supply water for the domestic, industrial, and agricultural uses in the Kaohsiung area. Due to the long-term sediment deposition at A-Kong-Tien Reservoir, a sediment dredge system is under construction at this reservoir. Thus, only Chen-Ching Lake Reservoir and Fengshan Reservoir play the most important roles in water supply at this moment. However, both Chen-Ching Lake Reservoir and Fengshan Reservoir are in the list of the most eutrophized reservoirs. There are several ways to evaluate the status of eutrophication of reservoirs. The single-index method and multi-variable modeling method are two of the most commonly methods for water quality analysis and eutrophication evaluation for the reservoirs in Taiwan. Based on the analytical results from previous studies, eutrophication results in the fast growing of seaweeds and they spread and cover the whole water bodies, which is called algal bloom. The type of seaweed causing eutrophication in Taiwan reservoirs are mainly Cyanobacteria, especially the commonly found Microcystis. They can not only cause some foul smells but release toxins into the water bodies. That endangers the safety of drinking water and affects the normal water usage. To prevent eutrophication, the nutrients loadings outside and inside of the reservoir areas must be reduced and controlled, which can effectively prohibit the growth of seaweeds. An air-pumping system can also be built for the same purpose. Regular water quality inspection should be carried out to monitor the fluctuation of water quality.

Eutrophication is a global problem affecting many inland and estuarine waters. Many wetlands on the Swan Coast Plain, in Western Australia, have undergone increasing nutrient enrichment since European settlement of the region in the 1850’s. Problems such as algal blooms and nuisance swarms of non-biting midges (Diptera; Chironomidae) are the consequence of nutrient enrichment in many of these wetlands. The restoration of these degraded wetlands, especially with respect to reducing nutrient enrichment, requires a range of comprehensive and effective techniques including catchment management, diversion or treatment of surface inputs and treatment of enriched sediments. Nitrogen and phosphorus, especially phosphorus, are not the only factors controlling algal biomass in water bodies, but they are the only elements that can be removed efficiently and economically. Internal P cycling from wetland sediments can initiate and sustain eutrophication and related algal blooms and nuisance midge problems even after external sources are diverted or reduced. The aim of this study was to identify an effective material to reduce sediment phosphorus release and thereby the phosphorus concentration of the water column. It was also important to determine the impact of the selected amendment material on phytoplankton and larval midge (chironomid) communities. A range of experiments at increasing scales, from bench-top, to microcosm to outdoor mesocosm experiments were designed to test three hypotheses: 1) Materials which have a high P sorption capacity, over a wide range of P solution concentrations, and low P release rate, are potentially suitable agents to reduce P in wetlands with enriched sediments by inactivating sediment P; 2) A reduction in the abundance of cyanobacteria caused by increasing the N:P ratio of an aquatic ecosystem results in a reduction in the density of nuisance species of Chironomidae. 3) Successful amendment of enriched sediments reduces P in the water column thereby reducing the total phytoplankton biomass and the related density of nuisance species of Chironomidae. The adsorption and desorption experiments were carried out under a range of pH values and P concentrations, with a number of materials including fly ash, red mud, precipitated calcium carbonate, crushed limestone and lime to determine the maximum adsorption capacity and affinity of these materials. A rang of P concentrations (0-1000 µg/L) simulated the P concentration of the water column in a range of wetlands of differing trophic status. Poor fits to the Langmuir equation occurred with both red mud and fly ash due to their high P content. A good fit occurred with lime, with a high P removal rate (90%-96%) over the same range. Fly ash and red mud were eliminated from further investigation due to the possibility that they might release phosphorus rather than absorb when P concentrations in surrounding environment were less than 300 µg/L or 200 µg/L respectively (concentrations which can occur in eutrophic systems). Among the three lime-based, redox-insensitive materials tested in the second mesocosm experiments, precipitated calcium carbonate (PCC) possessed the highest maximum adsorption capacity and lowest desorption rate under a range of pH values (6.2, 7.2 and 10) and P concentrations (0-12 000 µg/L), followed by crushed limestone and lime. The different maximum absorption capacities of the three materials appears to be mainly attributed to their particle size (surface area). Lime was chosen as the amendment material for further investigation because it was the only one of the three available in sufficient quantities within the timeframe of this study. Microcosm experiments showed that lime was effective in reducing sediment P release from intact sediment cores, and the ratio of TN:TP in the treatment cores increased over time compared to the control cores (in which TN: TP decreased slightly). In the first mesocosm experiment a significantly higher density of larval midges was found in the treatments than in the controls. The treatments were aimed to increase N:P ratio in the systems to reduce cyanobacteria and, subsequently, larval midge densities. However even though cyanobacteria were eliminated from the treatments, the nitrogen addition appeared to result in higher phytoplankton biomass overall, which fuelled an increase in larval midge densities. In the second mesocosm experiment, the addition of lime to enriched sediments resulted in a reduction in P in the water column. This reduction was accompanied by a reduction in total phytoplankton biomass, the absence of cyanobacteria, and a less abundant and more species - diverse chironomid fauna in the treatment mesocosms. Sediment P fractionation undertaken for both the microcosm and mesocosm experiments showed that most of the phosphorus adsorbed by lime was in the labile fraction (NH3Cl extractable P and NaOH extractable P). Phosphorus in the HCl extractable fraction was also found to be higher in the treatments due to the presence of inert mineral P in the lime than the formation of new hydroxyapatite from adsorbed P. The two mesocosm experiments suggested that larval midges were non-selective feeders, responding to total phytoplankton biomass, rather than the presence of cyanobacteria. Dissolved oxygen and predation also influenced larval midge densities. In summary, although lime appeared to be a useful material for reducing P release from enriched sediments under controlled laboratory conditions, the effect under field conditions was not as definitive. Further work is required to more fully determine the conditions under which sediment remediation may be used as a means of controlling sediment P release and associated high densities of larval chironomids.

Over the past two decades, Lake Winnipeg has been experiencing increasingly rapid eutrophication, and large cyanobacterial blooms now form in the North Basin in most years in late summer or fall. Cyanobacteria are considered a relatively poor food source compared with other phytoplankton, but the impacts of these blooms upon the primary consumers in the lake have not previously been researched. A microscopic analysis of whole water samples found cyanobacteria to be scarcely present in summer 2012, with nitrogen-fixing and non-fixing cyanobacteria comprising 11.2% and 8.4% of the basin-wide biovolume, respectively, and all but absent in fall. Gut content analysis of chironomids found that cyanobacteria made up an almost negligible part of their diet. Stable isotope analysis revealed that nitrogen-fixing cyanobacteria reduced phytoplankton δ15N values, and that this same reduction could be traced through the zooplankton, but not down to the sediments or chironomids.

Over the past two decades, Lake Winnipeg has been experiencing increasingly rapid eutrophication, and large cyanobacterial blooms now form in the North Basin in most years in late summer or fall. Cyanobacteria are considered a relatively poor food source compared with other phytoplankton, but the impacts of these blooms upon the primary consumers in the lake have not previously been researched. A microscopic analysis of whole water samples found cyanobacteria to be scarcely present in summer 2012, with nitrogen-fixing and non-fixing cyanobacteria comprising 11.2% and 8.4% of the basin-wide biovolume, respectively, and all but absent in fall. Gut content analysis of chironomids found that cyanobacteria made up an almost negligible part of their diet. Stable isotope analysis revealed that nitrogen-fixing cyanobacteria reduced phytoplankton δ15N values, and that this same reduction could be traced through the zooplankton, but not down to the sediments or chironomids.

Algal bloom can significantly impact reverse osmosis desalination process and reduce the drinking water production. In 2008, a major bloom event forced several UAE reverse osmosis plants to stop their production, and in this context, a better understanding of UF membrane fouling caused by algal organic matter (AOM) is needed, in order to adjust the filtration conditions during algal bloom events. Polymeric MF/UF membranes are already widely used for RO pretreatment, but ceramic UF membranes can also be an alternative for the filtration of marine algal solutions. The fouling potential of the Red Sea and the Arabian Sea, sampled at different seasons, along with four algal monocultures grown in laboratory, and one mesocosm experiment in the Red Sea was investigated. Algal solutions induce a stronger and more irreversible fouling than terrestrial humic solution, toward ceramic membrane. During algal bloom events, this fouling is enhanced and becomes even more problematic at the decline phase of the bloom, for a similar initial DOC. Three main mechanisms are involved: the formation of a cake layer at the membrane surface; the penetration of the algal organic matter (AOM) in the pore network of the membrane; the strong adhesion of AOM with the membrane surface. The last mechanism is species-specific and metal-oxide specific. In order to understand the stronger ceramic UF fouling at the decline phase, AOM quality was analyzed every two days. During growth, AOM is getting enriched in High Molecular Weight (HMW) structures (> 200 kDa), which are mainly composed by proteins and polysaccharides, and these compounds seem to be responsible for the stronger fouling at decline phase. In order to prevent the fouling of ceramic membrane, coagulation-flocculation (CF) using ferric chloride was implemented prior to filtration. It permits a high removal of HMW compounds and greatly reduces the fouling potential of the algal solution. During brief algal bloom events, CF should be implemented prior to UF to protect the membranes. A comparison between polymeric and ceramic UF membranes showed that ceramic membrane suffers from a stronger fouling but permits a better removal of DOC and particularly HMW compounds.

Excess nutrients from human activity trigger toxic cyanobacterial and algal blooms, creating expansive hypoxic dead zones in lakes, damaging ecosystems, hurting local economies, undermining food and water security, and directly harming human health. To identify when and where nutrients limit phytoplankton and cyanobacterial growth, and cyanotoxin concentrations across Utah Lake, USA we conducted four in-situ bioassay studies (563 cubitainers or experimental units) that experimentally added N, P or N+P over the spring, early summer, summer, late summer, and fall in lake water from the top 20 cm of the water column. For our purpose, we defined total phytoplankton as all prokaryotic or eukaryotic organisms containing chlorophyll-a. We evaluated changes in chlorophyll-a and phycocyanin concentrations; the abundance of cyanobacterial species and total phytoplankton species or divisions; cyanotoxin concentrations of the microcystin, anatoxin-a, and cylindrospermopsin; DIN, SRP, TP, and TN concentrations; and other water chemistry parameters. We found that the nutrient limitation of cyanobacteria, and to a lesser extent phytoplankton, was influenced by season and space. Cyanobacteria were often co-limited in the spring or early summer, limited by a single nutrient in the summer, and not limited by N or P in the late summer and fall. Alternatively, phytoplankton were co-limited from the summer into the fall in the main body of the lake and either N limited or co-limited continually in Provo Bay. Microcystis, Aphanocapsa, Dolichospermum, Merismopedia, and Aphanizomenon spp., and Aulacoseira and Desmodesmus spp. and two taxonomical categories of algae (i.e., unicellular and colonial green algae) were primarily associated with cyanobacteria and phytoplankton nutrient limitations. Concentrations of the three cyanotoxins demonstrated a seasonal signal and loosely followed the growth of specific cyanobacteria but was not dependent on total cyanobacterial cell density. The DIN and SRP were biologically available in all water and nutrient treatments with nutrient concentrations declining over the incubation period, suggesting that nutrient levels were not oversaturated. Our results offer insights into specific nutrient targets, species, and, and cyanotoxins to consider in the future to manage Utah Lake.

With the global rise in frequency of harmful algal blooms in estuarine environments comes an increase in prevalence of toxic metabolites, such as microcystin (MC), that some of the cyanobacteria involved will produce. At high concentrations, MC may accumulate in consumer tissues and have deleterious effects on organisms; however impacts of the toxin on aquatic living resources at ecologically relevant concentrations have not been widely documented. We analyzed the effects of MC on juveniles of five fish species from the James River, Virginia to determine if MC has the potential to impede growth. Using three separate experimental approaches, it was shown that exposure to concentrations of the toxin currently observed in the James River estuary do not appear to significantly impact the growth or survivorship of tested fish species. Extraneous factors in parts of the study led to an inability to draw clear conclusions on mortality or growth impacts; however it is evident from the experiments that at least some of the fish species have biological mechanisms in place that allow them to effectively eliminate the toxin from their systems. An ability to extricate the toxin suggests the possibility for fishes to withstand MC exposures and sustain few negative health impacts at low MC concentrations.

Karenia brevis is a toxic marine dinoflagellate species that blooms almost every year in the Gulf of Mexico. These blooms have had devastating effects on local economies, as well as on the fauna of the area. The ECOHAB:Florida project was founded to study the population dynamics and trophic impacts of K. brevis. The project included a series of monthly hydrographic offshore research cruises, as well as monthly surveys of a transect along the 10-meter isobath of the West Florida Shelf. This study focused on data from the alongshore transect over a three-year period (1999-2001). Physical parameters (temperature, salinity, and density) and chemical parameters (particulate carbon, nitrogen, and phosphorus; dissolved inorganic nitrogen and phosphorus) of the West Florida Shelf were analyzed. The amount of chlorophll a and the location and amount of K. brevis cells present were also examined. Clear spatial, seasonal, and interannual patterns in the hydrographic parameters, particulate matter (C, N, P), dissolved inorganic nutrient (nitrite, nitrate, and phosphate), and chlorophyll a concentrations were found. At various times throughout the study, the location of K. brevis blooms was influenced by all of these factors except for the amount of dissolved inorganic nutrients. There were differences in particulate matter ratios present in bloom and non-bloom periods. No clear-cut differences in dissolved inorganic nutrients between bloom and non-bloom periods were found. Finally, relationships between the biological indicators of blooms (i.e., chlorophyll a) and the aforementioned physical and chemical parameters were found.

Optical research has shown that Karenia brevis has distinct spectral characteristics, yet most studies have focused exclusively on absorption and chemical properties, ignoring the size, shape, internal structure, and orientation, and their effect on scattering properties. The application of a new spectral interpretation model to K. brevis is shown to provide characterization of unique spectral information, not previously reported, through the use of scattering and absorption properties. The spectroscopy models are based on light scattering and absorption theories, and the approximation of the frequency-dependent optical properties of the basic constituents of living organisms. The model uses the process of mathematically separating the cell into four components, while combining their respective scattering and absorption properties, and appropriately weighted physical and chemical characteristics. The parameters for the model are based upon both reported literature values, and experimental values obtained from laboratory grown cultures and pigment standards. Measured and mathematically derived spectra are compared to determine the adequacy of the model, contribute new spectral information, and to establish the proposed spectral interpretation approach as a new detection method for K. brevis. Absorption and scattering properties of K. brevis, such as cell size/shape, internal structure, and chemical composition, are shown to predict the spectral features observed in the measured spectra. This research documents for the first time the exploitation of every spectral feature produced by the interaction of light with the cellular components and their contribution to the total spectrum of a larger (20-40 µm) photosynthetic eukaryote, K. brevis. Overall, this approach could eventually address the detection deficiencies of current optical detection applications and facilitate the understanding of K. brevis bloom ecology.