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Previous issue date: 2017-07-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O objetivo deste estudo foi compreender os mecanismos moleculares associados ? resist?ncia de ovinos ?s infec??es por nematoides gastrintestinais. Foi comparado o transcriptoma da mucosa do abomaso de 17 ovinos mesti?os ? Santa In?s e ? Dorper, previamente classificados como infectados (resistentes e suscept?veis) e n?o infectados, distribu?dos em dois sistemas de alimenta??o (ad libitum e alimenta??o restrita) em resposta a infec??o por Haemonchus sp., utilizando a tecnologia RNA-Seq. A prepara??o das bibliotecas, o sequenciamento do genoma e a an?lise de dados foram realizadas no Laborat?rio de Biotecnologia Animal - ESALQ, Piracicaba, Brasil. A m?dia de sequ?ncias por amostra antes e depois da filtragem foi de 12.522.573 e 9.626.457, respectivamente, e a m?dia da taxa de mapeamento das leituras filtradas contra o genoma de refer?ncia do ovino Oar_v4.0 foi de 79,66%. Foram identificados como diferencilamente expressos (DE) 421 e 1123 genes quando comparado os animais infectados e n?o infectados, dentro do grupo de alimenta??o restrita e ad libitum, respectivamente. Quando avaliados os infectados inseridos na alimenta??o ad libitum versus alimenta??o restrita, 13 genes foram DE. Quando avaliados os animais resistentes e suscept?veis com efeito fixo de alimenta??o, apenas 36 genes foram DE. E por fim, comparando-se os animais infectados versus os controles tendo a alimenta??o como efeito fixo, foram identificados 881 genes DE. A an?lise de enriquecimento funcional mostrou que alguns termos do Gene Ontology foram significativamente enriquecidos (valor p ajustado<0,05). Nossos achados sugerem que al?m dos genes que participam diretamente do sistema imunol?gico, genes que participam de outras vias biol?gicas como o metabolismo do ?cido araquid?nico, via de sinaliza??o e sistema de complemento s?o essenciais na resposta do hospedeiro a Haemonchus contortus. Al?m disso, a alimenta??o n?o apresentou um efeito significativo no perfil de express?o g?nica dos animais infectados e n?o infectados, mostrando que a diferen?a entre a express?o dos genes foi devido ? infec??o por H. contortus. / The aim of this study was to understand molecular mechanisms underlying host resistance. We compared the abomasal mucosal transcriptome of 17 crossbred ? Santa In?s and ? Dorper lambs, previously classified as infected (resistant and susceptible) and uninfected distributed in two feeding system (ad libitum and restrict feed) in response to Haemonchus sp. infection using RNA-Seq technology. The libraries preparation, genome sequencing and sequence analyses were performed at the Laboratory of Animal Biotechnology - ESALQ, Piracicaba, Brazil. The average of reads per sample before and after filtering was 12.522.573 and 9.626.457, respectively, and the average of mapping rate of filtered reads against to Ovis aries Oar_v4.0 reference genome assembly was 79.66%. 421 and 1123 differentially expressed (DE) genes were identified when compared to infected and uninfected animals, within the restricted and ad libitum feed groups, respectively. When evaluated the infected animals inserted in the ad libitum feed versus restricted feeding, 13 genes were DE. When evaluated the resistant and susceptible animals with fixed feeding effect, only 36 genes were DE. Finally, by comparing infected versus control animals with feed as a fixed effect, 881 DE genes were identified. Functional enrichment analysis showed that some Gene Ontology terms were significantly enriched (adjusted p-value <0.05). Our findings suggest that in addition to genes that participate directly in the immune system, genes that participate in other biological pathways such as arachidonic acid metabolism, signaling pathway and complement system, for example, are essential in the host's response to Haemonchus contortus, generating a greater sheep resistance. In addition, feeding did not have a significant effect on the gene expression profile, showing that the difference between gene expressions was due to H. contortus infection.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24075 |
| Date | 31 July 2017 |
| Creators | Silva, Iasmim Santos Mangabeira e |
| Contributors | 24554066878, Monteiro, Jomar Patricio, 17396558859, Coutinho, Luiz Lehmann, 06284487835, Medeiros, Henrique Rocha de, Medeiros, Lilian Giotto Zaros de |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM PRODU??O ANIMAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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