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Previous issue date: 2016-02-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The objective of this research was to evaluate the effect of the arthropod growth regulator fluazuron in the energetic metabolism of Rhipicephalus microplus. 6 stabled cattle divided into control and treated groups containing three animals each. A commercial formulation containing the active compound fluazuron was administered topically in the treated group. The engorged female ticks were collected from some days before (day 0) and after treatment of animals (4, 8, and 15 days). After collecting the engorged females, the following biological parameters were analyzed: weight of the engorged female, weight of the female after oviposition, laying period, laying weight, egg production rate, reproductive efficiency index, percentage of hatching and nutritional index. It was also evaluated the metabolic profile: in the hemolymph (concentration of glucose, total protein and organic acids - oxalic, pyruvic and lactic, enzymatic activity of LDH, ALT and AST); in the fat body (glycogen concentration and percentage of variation of neutral lipids); in eggs (concentration of glucose and total protein content, percentage change of neutral lipids and enzymatic activities of ALT and AST); and in the cuticle of engorged female ticks (percentage change of neutral lipids). Secondly, female immersion test was conducted to assess the activities of carboxylesterases enzymes, monooxygenases (MFO) and glutathione-S-transferase (GST) in sensitive strain of eggs and larvae (Porto Alegre, RS) compared with resistant strain (Jaguar, RS). It also assessed the detoxification enzymes in the fat body of engorged females of sensitive strain. The results showed that all the biological parameters showed lower results when compared to the control group after the exposure to fluazuron, except the weight of the female after oviposition. Although an increase was observed in the concentration of glucose in hemolymph on the 8th day and glycogen in the fat body in 15th day, there was no significant difference in the comparison of means between control and treated day-to-day. The concentration of lactic acid gradually increased in the treated group over time due to treatment, indicating increased anaerobic metabolism. There was no significant difference in the percentage change of neutral lipids in fat body, cuticle and eggs in any of the time intervals. No significant difference was observed in the activity of transaminases in the hemolymph, but it was observed in eggs after the treatment on the 8th day. The activity of MFO was higher in the comparison between the resistant and sensitive strains in eggs, but in larvae, the increase was observed in carboxylesterases in the resistant strain. In females, MFO showed high activity in the fat body as a function of fluazuron exposure, especially on day 4 in the treated group. Increases in transaminase activity and detoxification enzymes in females and eggs were associated with possible cell damage and trying to metabolism of active principle-tested. For the first time, a research about the metabolic effects of fluazuron has been conducted, focusing on different biochemical aspects, demonstrating that its effectiveness is related to a number of metabolic changes, leading to a parasite homeostasis breaking. / O objetivo desta pesquisa foi avaliar os perfis metab?lico-energ?tico de carrapatos Rhipicephalus microplus expostos ao fluazuron. 6 bovinos foram estabulados e divididos em grupos controle e tratado, contendo 3 animais cada. Uma formula??o comercial contendo o princ?pio-ativo fluazuron foi administrada por via t?pica nos animais do grupo tratado. F?meas ingurgitadas foram coletadas em dias determinados: antes (dia 0) e ap?s o tratamento dos animais (4, 8 e 15 dias). Ap?s a coleta das f?meas ingurgitadas, foram analisados os seguintes par?metros biol?gicos: peso da f?mea ingurgitada, peso da quen?gina, per?odo de postura, peso da postura, ?ndice de produ??o de ovos, efici?ncia reprodutiva, percentual de eclos?o e ?ndice nutricional. Foi avaliado tamb?m o perfil metab?lico: na hemolinfa (concentra??o de glicose, prote?nas totais e ?cidos org?nicos - ox?lico, pir?vico e l?tico, atividade enzim?tica de LDH, ALT e AST), no corpo gorduroso (concentra??o de glicog?nio e percentual de varia??o de lip?dios neutros), nos ovos (concentra??o de glicose, prote?nas totais, percentual varia??o de lip?dios neutros e atividade enzim?tica de ALT e AST), e na cut?cula das f?meas ingurgitadas (percentual de varia??o de lip?dios neutros). Num segundo momento, realizou-se teste de imers?o de f?meas ingurgitadas para posterior avalia??o das atividades das enzimas carboxilesterases, monooxigenases (MFO) e glutationa-S-transferase (GST) em ovos e larvas de diferentes cepas: sens?vel (Porto Alegre, RS) e cepa resistente (Jaguar, RS) ao fluazuron. Avaliou-se tamb?m as enzimas de detoxifica??o no corpo gorduroso de f?meas ingurgitadas de cepa sens?vel. Na an?lise dos resultados, todos os par?metros apresentaram resultados inferiores, quando comparados ao grupo controle, em fun??o da exposi??o ao fluazuron, exceto o peso da quen?gina. Embora tenha sido observado um aumento na concentra??o de glicose hemolinf?tica no 8? dia e glicog?nio no corpo gorduroso no 15?dia, n?o houve diferen?a significativa na compara??o das m?dias entre controle e tratado dia-a-dia. A concentra??o do ?cido l?tico apresentou aumento progressivo significativo no grupo tratado ao longo do tempo em fun??o do tratamento, indicando aumento do metabolismo anaer?bio. N?o houve diferen?a significativa para as varia??es percentuais de lip?dios neutros no corpo gorduroso, cut?cula e ovos em nenhum dos dias de avalia??o. Embora n?o tenha sido observada diferen?a significativa nas atividades das transaminases na hemolinfa, observou-se aumento significativo nos ovos ap?s o tratamento no 8?dia. A atividade da MFO foi maior, na compara??o entre as cepas resistente e sens?vel, nos ovos, e com rela??o ?s larvas; o aumento observado foi nas carboxilesterases da cepa resistente. Nas f?meas, a MFO apresentou atividade elevada no corpo gorduroso em fun??o da exposi??o ao fluazuron, principalmente no 4?dia no grupo tratado. Os aumentos na atividade das transaminases e das enzimas de detoxifica??o nas f?meas ingurgitadas e nos ovos foram associados a poss?veis danos celulares e ? tentativa de metaboliza??o do princ?pio-ativo testado. Pela primeira vez, foi realizado um estudo acerca dos efeitos metab?licos do fluazuron, enfocando diferentes aspectos bioqu?micos, evidenciando que a sua efic?cia est? relacionada a diversas altera??es metab?licas, levando a uma quebra da homeostase do carrapato
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1804 |
| Date | 22 February 2016 |
| Creators | Gaud?ncio, Fabr?cio Nascimento |
| Contributors | Silva, Jairo Pinheiro da, Angelo, Isabele da Costa, Fonseca, Adivaldo Henrique da, Cid, Yara Peluso, Monteiro, Caio M?rcio de oliveira, Melo, Raquel Moreira Pires dos Santos |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7 REFER?NCIAS BIBLIOGR?FICAS ABBAS, R.Z.; ZAMAN, M. A.; COLWELL, D.D.; GILLEARD, J.; IQBAL, Z. Acaricide resistance in cattle ticks and approaches to its management: The state of play. VeterinaryParasitology, v.203, p.6?20, 2014. ANDREOTTI, R. Empresa Brasileira de Pesquisa Agropecu?ria. Embrapa Gado de Corte. Minist?rio da Agricultura, Pecu?ria e Abastecimento. Situa??o atual da resist?ncia do carrapato-do-boi Rhipicephalus (Boophilus) microplus aos acaricidas no Brasil. Dados eletr?nicos. ? Campo Grande, MS: Embrapa Gado de Corte, 2010. 36 p. ANGELO, I.C.; G?LO, P.S.; CAMARGO, M.G.; KLUCK, G.E.G.; FOLLY, E.; BITTENCOURT, V. R. E. P. Haemolymph protein and lipid profile of Rhipicephalus (Boophilus) microplus infected by fungi. Transboundary and Emerging Diseases, v.57, p.79?83, 2010. ANGELO, I.C., G?LO, P.S., PERINOTTO, W.M.S., CAMARGO, M.G., QUINELATO, S., S?, F.A. AND PONTES, E.G. Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections, Parasitology Research, v.112, p.501-509, 2013. ANGELO, I.C.; TUNHOLI-ALVES, V.M.; TUNHOLI, V.M.; PERINOTTO, W.M.S.; G?LO, P.S.; CAMARGO, M.G.; QUINELATO, S.; PINHEIRO, J.; BITTENCOURT, V.R.E.P. Physiological changes in Rhipicephalus microplus (Acari: Ixodidae) experimentally infected with entomopathogenic Fungi, Parasitology Research, v.114, p.219-225, 2015. Approved Recommendation (1985) on IFCC Methods for the Measurement of Catalytic Concentration of Enzymes. Part 3: IFCC Method for Alanine Aminotransferase (EC 2.6.1.2). Journal of clinical chemistry and clinical biochemistry, v.24, p.481-495, 1986. BARROS, A.T.M.; SCHUMAKER, T.T.S.; KOLLER,W.W.; KLAFKE, G.M.; ALBUQUERQUE, T.A.; GONZALEZ, R. Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil. Revista Brasileira de Parasitologia Veterin?ria, v. 22, n. 1, p. 136-142, 2013. BARROS-BATTESTI, D.M.; ARZUA, M.; BECHARA, G.H. Carrapatos de import?ncia m?dico-veterin?ria da Regi?o Neotropical: Um guia ilustrado para identifica??o de esp?cies. S?o Paulo: Vox/ International Consortium on Ticks and Tick-borne Diseases (ICTTD-3) /Butantan, 2006. 223p. BENETTI, G.F. Oviposition of Boophilus microplus (Canestrini, 1987) (Acarina: Ixodidae). I. Influence of tick size on egg production. Acarologia, v.16, n.1, p.52-61, 1974. BENNETT, G.A.; SHOTWELL, O.L. Haemolymph lipids of healthy and diseased japanese beetle larvae. JournalofInsectPhysiology, v.18, p.53-62, 1972. 61 BITTENCOURT, A. J. Boophilus microplus (Canestrini, 1887): infesta??es artificiais, biologia da fase n?o parasit?ria e preval?ncia em caprinos e eq?inos. 1990. 87f. Disserta??o de Mestrado, Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. BLIGH, E.G.; DYER, W.J. A rapid method of total lipid extraction and purification. Journal of Biochemistry Physiology, v.37, p. 911-913, 1959. BLOMQUIST, G.J.; JACKSON, L.L. Chemistry And Biochemistry Of Insect Waxes. Progress In Lipid Research, v.17, p. 319-345, 1979. BOOTH, T.F. Wax lipid secretion and ultrastructural development in the egg-waxing (Gene's) organ in ixodid ticks. TissueCell, v. 21, p. 113-22, 1989. BORGES, L. M. F.; CARNEIRO, J. R.; GOMES, A. G.; MOREIRA, P. C. Influ?ncia do peso inicial e da esta??o do ano na convers?o em ovos de f?meas de Boophilus microplus (Acari: Ixoxidae). Ci?ncia Animal Brasileira, v. 2, n. 2, p. 127-131, 2001. BRASIL. Minist?rio da Sa?de. Funda??o Oswaldo Cruz. Metodologia para qualifica??o de atividades de enzimas relacionados com a resist?ncia a inseticidas em Aedes aegypti. Bras?lia: Minist?rio da Sa?de, 2006, 128p. BULL, M.S.; SWINDALE, S.; DOVEREND, D.; HESS, E.A. Supression of Boophilus microplus populations with fluazuron; an acarine growth regulator. Australian Veterinary Journal, v. 74, p.468?470, 1996. CABRAL, G.A. Lipids as bioeffectors in the immune system. Life Sciences, v.77, p. 1699?1710, 2005. CALLIGARIS, I.B.; OLIVEIRA, P.R.; ROMA, G.C.; BECHARA, G.H.; CAMARGO-MATHIAS, M.I. Action of the Insect Growth Regulator Fluazuron, The Active Ingredient of the Acaricide Acatak VR, in Rhipicephalus sanguineus nymphs (Latreille, 1806) (Acari: Ixodidae). Microscopy Research And Technique, n. 76, p. 1177?1185, 2013. CAMPOS, E.; MORAES, J.; FA?ANHA, A.R.; MOREIRA, E.; VALLE, D.; ABREU, L.; MANSO, P.P.A.; NASCIMENTO, A.; PELAJO-MACHADO, M.; LENZI, H.; MASUDA, A.; VAZ JR, I.S.; LOGULLO, C. Kinetics of energy source utilization in Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) embryonic development. Veterinary Parasitology, v.138, p. 349?357, 2006. CHANG, R.; MOQUIST, P.; FINNEY, N.S. Chemical synthesis of UDP-4-O-methyl-GlcNAc, a potential chain terminator of chitin synthesis. Carbohydrate Research, v. 339 , p.1531?1536, 2004. CORDOV?S, C.O. Carrapato: controle ou erradica??o. 1? ed. Gua?ba: Agropecu?ria, 1997. 176p. 62 CHERRY, L. M. Utilization of cholesterol by the cattle tick Boophilus microplus: cholesterol economy in the engorged female adult. Insect Biochemistry, v.6, p.587- 594, 1976. CITRONI, D.; D?AGOSTINO, B. I.; MARTINS, S.; SCHMID, H. R.; JUNQUEIRA, P. Efficacy of fluazuron against infestations with Argentinean B. microplus, 17th International Conference of the WAAVP, Copenhagen, p. 15 ? 19, 1999. COHEN, E. Chitin Biochemistry: Synthesis and inhibition. Annual Review of Entomology, v. 32, p.71-93, 1987. CRUZ, B.C.; TEIXEIRA, W.F.P.; MACIEL, W.G.; FELIPPELLI, G.; F?VERO, F.C.; CRUZ, A.C.; BUZZULINI, C.; SOARES, V.E.; GOMES, L.V.C.; LOPES, W.D.Z.; OLIVEIRA, G.P.; COSTA, A.J. Research in Veterinary Science, n.97, p.80-84, 2014. DA GL?RIA, M.A. Estudos preliminares para avalia??o do uso de compostos reguladores de crescimento no controle de R. (Boophilus) microplus. Disserta??o (Mestrado em Medicina Veterin?ria), Universidade Federal Rural do Rio de Janeiro, Serop?dica, 1988. DA GL?RIA, M. A.; FACCINI, J. L. H.; DAEMON, E.; GRISI, L. Biologia comparativa da fase n?o parasit?ria de estirpes de B. microplus (Can., 1887) resistente e sens?vel a carrapaticida em condi??es de laborat?rio. Revista Brasileira de Parasitologia Veterin?ria, v. 2, n. 2, p. 79-84, 1993. DAVIS, M-T. B. Critical temperature and changes in cuticular lipids in the rabbit tick, Haemaphysalis leporispalustris. Journal of Insect Physiology, v. 20, p.1087 to 1100, 1974. DE PAULA, P.H.; FURLONG, J. Comportamento de queda de f?meas ingurgitadas do carrapato Boophilus microplus. Ci?ncia Rural, v.32, n.4, p.627-631, 2002. DEL PINO, J.C.; TRINDADE, V.M.T.; GUMA, F.; BERNARD, E.A. Biochemical studies of ovary glycoproteins of the cattle tick Boophilus microplus. Insect Biochemistry, v.19, n.7, p.657-661, 1989. DING, Y.; ORTELLI, F.; ROSSITER, L.C.; HEMINGWAY, J.; RANSON, H. The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression. Profiles. BMC Genomics, v.4, n.35, 2003. DRUMMOND, R. O.; ERNEST, S. E.; TREVINO, J. L.; GLADNEY, W.J.; GRAHAM, O. H. Boophilus annulatus and Boophilus microplus: Laboratory test of insecticides. Journal of Economic Entomology, v. 66, n.1, p. 130-133, 1973. DUSCHER, G.G.; GALINDO, R.C.; TICHY, A.; HUMMEL, K.; KOCAN, K.M.; DE LA FUENTE, J. Glutathione S-transferase affects permethrin detoxification in the brown dog tick, Rhipicephalus sanguineus. Ticks and Tick-borne Diseases, v. 5, p.225?233, 2014. ECK, W.H. Mode of action of two benzoylphenylureas as inhibitors of chitin synthesis in insects. Insect Biochemistry, v.9, p.295-300, 1979. 63 ENAYATI, A.A.; ASGARIAN, F.; SHARIF, M.; BOUJHMEHRANI, H.; AMOUEI, A.; VAHEDI, N.; BOUDAGHI, B.; PAIZAK, N.; HEMNGWAY, J. Propetamphos resistance in Rhipicephalus bursa (Acari, Ixodidae). Veterinary Parasitology, n.162, p.135?141, 2009. ENAYATI, A.A.; ASGARIAN, F.; AMOUEI, A.; SHARIF, M.; MORTAZAVI, H.; BOUJHMEHRANI, H.; HEMINGWAY, J. Pyrethroid insecticide resistance in Rhipicephalus bursa (Acari, Ixodidae). Pesticide Biochemistry and Physiology, v.97, p.243?248, 2010. FAO (Food and Agriculture Organization), 2004. Resistance Management and Integrated Parasite Control in Ruminants ? Guidelines, Module 1 ? Ticks: Acaricide Resistance: Diagnosis, Management and Prevention. Food and Agriculture Organization, Animal Production and Health Division, Rome, pp. 25?77. FREITAS, D.R.J.; ROSA, R.M.; MORAES, J.; CAMPOS, E.; LOGULLO, C.; DA SILVA VAZ JR., I.; MASUDA, A. Relationship between glutathione S-transferase, catalase, oxygen consumption, lipid peroxidation and oxidative stress in eggs and larvae of Boophilus microplus (Acarina: Ixodidae). Comparative Biochemistry and Physiology, Part A, v.146, p.688?694, 2007. FURLONG, J. Controle do carrapato dos bovinos na regi?o Sudeste do Brasil. Belo Horizonte: Escola de Veterin?ria da UFMG, 1993. (Boletim T?cnico, n. 8). FURLONG, J.; PRATA, M. C. A.; MARTINS, J. R. O carrapato dos bovinos e a resist?ncia: temos o que comemorar? A Hora Veterin?ria, v.27, n.159, p. 26-32, 2007. FURLONG, J.; SALES, R.O. Controle estrat?gico de carrapatos no bovino de leite: uma revis?o. Revista Brasileira de Higiene e Sanidade Animal, v.01, n.02, p.44-72, 2007. GALAY, R.L; UMEMIYA-SHIRAFUJI, R.; MOCHIZUKI, M.; FUJISAKI, K.; TANAKA, T. Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet. Parasitology International, v. 64, p.182?189, 2015. GAUD?NCIO, F.N.; TUNHOLI-ALVES, V.M.; LIMA, M.G.; G?LO, P.S.; ?NGELO, I.C.; CASTRO, R.N.; FONSECA, A.H.; SCOTT, F.B.; PINHEIRO, J. Alterations in the oxidative metabolism of Rhipicephalus (Boophilus) microplus ticks in response to exposure to the insect growth regulator fluazuron. Brazilian Journal of Veterinary Parasitology, vol.25, n.1, p.54-60, 2016. GELLA, F.J.; OLIVELLA, T.; CRUZ PASTOR, M.; ARENAS, J.; MORENO, R.; DURBAN, R.; G?MEZ, J.A. A simple procedure for routine determination of aspartate aminotransferase and alanine aminotransferase with pyridoxal phosphate. ClinicaChimica Acta, v.153, p.241-247, 1985. GONZALES, J. C., SILVA, N. R., FRANCO, N. A vida livre do Boophilus microplus. Arquivos da Faculdade de Veterin?ria da UFRGS, v.3, p. 21-28, 1974. 64 GONZALES, J.C. O controle dos carrapatos dos bovinos. Porto Alegre: Sulina, 1975. 104p. GORNALL, A.G.; BARDAWILL, C.S.; DAVID, M.M. Determination of s?rum proteins by means of the Biuret reaction. The Journal of Biological Chemistry, v.177, p.751-766, 1949. GRAF, J-F. The Role of Insect Growth Regulators in Arthropod Control. Parasitology Today, v.9, n.12, p. 471-474, 1993. GRAF, J-F.; GOGOLEWSKI, R.; LEACH-BING, N.; SABATINI, G.A.; MOLENTO, M.B.; BORDIN, E.L.; ARANTES, G.J. Tick control: an industry point of view, Parasitology, v.129, S427?S442, 2004. GRISI, L.; LEITE, R.C.; MARTINS, J.R.S.; BARROS, A.T.M.; ANDREOTTI, R.; CAN?ADO, P.H.D.;L?ON, A.A.P.;PEREIRA, J.B.; VILLELA, H.S. Reassessment of the potential economic impact of cattle parasites in Brazil. Revista Brasileira de Parasitologia Veterin?ria, v.23, n.2, p. 150-156, 2014. GUDDERRA, N.P.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Hemolymph proteins in ticks. Journal of Insect Physiology, v. 48, p. 269?278, 2002. GUIZZO, M.G.; ABREU, L.; MASUDA, A.; LOGULLO, C.; VAZ JUNIOR, I.S. Metabolismo de biomol?culas na embriog?nese do carrapato Rhipicephalus (Boophilus) microplus. Acta Scientiae Veterinariae. v.40, n.1, p.1-12, 2012. HACKMAN, R.H.; GOLDBERG, M. Comparative study of some expanding arthropod cuticles: the relation between composition, structure and function. Journal of Insect Physiology, v.33, n.1, p39-50, 1987 HE, H.; CHEN, A. C.; DAVEY, R. B.; IVIE, G. W.; WAGNER, G. G.; GEORGE, J. E. Sequence analysis of the knockdown resistance-homologous regions ot the para-type sodium channel gene from pyrethroid-resistance Boophilus microplus (Acari: Ixodidae). Journal of Medical Entomology, v.36, n.5, p.539-543, 1999. HEMINGWAY, J., 1998. Field and laboratory manual for the mechanistic detection of insecticide resistance in insects. WHO-CTD/MAL/98.6, 35p. HODGSON, E. The significance of cytochrome P-450 in insects. Insect Biochemistry, v.13, n.3, p. 237-246, 1983. INCHEM (Chemical Safety Information from Intergovernmental Organizations). Disponivel em: www.inchem.org/documents/jmpr/jmpmono/v2005pr14.pdf. Acesso em 15 de fev. 2016. IWUALA, M.; UMEZURIKE, G.M.; NWADIOGBU, N. A qualitative study of the lipid components of eggs of Boophilus decoloratus KOCH (Ixodoidea Ixodidae). Acarologia, v.21, p. 3-4, 1979. 65 KAWOOYA, J.K.; LAW, J.H. Role of lipophorin in lipid transport to insect egg. Journal of Biological Chemistry, v. 263, p. 8748-8753, 1988. KLOWDEN, M.J. 2007. Chapter 2 - Integumentary Systems, p.75-135. In: KLOWDEN, M.J. Physiological Systems in Insects (Second Edition), London: Academic Press. KRAMER, K.J.; KOGA, D. Insect chitin - Physical state, synthesis, degradation and metabolic regulation. Insect Biochemistry. v.16, n.6, p.851-877, 1986. LEES, A.D.; BEAMENT, J.W.L. An egg-waxing organ in ticks. Quarterly Journal of Microscopical Science, v. 89, p. 291-333, 1948. LEVENBOOK L, BOCTOR FN, FALES HM. Biochemical studies of tick embryogenesis: free sugars in adult haemolymph and during embryogenesis of Dermacentor andersoni. Journal of Insect Physiology, v.26, n.6, p.381-383, 1980. LOGULLO, C.; WITOLA, W.H.; ANDRADE, C.; ABREU, L.; GOMES, J.; VAZ JR., I.S.; IMAMURA, S.; KONNAI, S.; OHASHI, K.; ONUMA, M. Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, v. 161, p.261?269, 2009. MARECHAL, E.; RIOU, M.I.; KERBOEUF, D.; BEUGNET, F.; CHAMINADE, P.; LOISEAU, P.M. Membrane lipidomics for the discovery of new antiparasitic drug targets. Trends in Parasitology, v.27, n.11, 2011. MARKWELL, M.A.K.; HAAS, S.M.; BIEBER, L.L.; TOLBERT, N.E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analitical Biochemistry, v.87, p.206?210, 1978. MEL?NDEZ, R. D.; CORONADO, A.; MUJICA, F.; CERUTTI,F; MOSQUERA, O. Levels of natural resistance two Boophilus microplus (Acari: Ixodidae) in Corora breed bulls. Revista de Biologia Tropical, v. 46, n. 3, p. 691-696, 1998. MELO, R.M.P.S. Morfologia e Biologia de Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) Submetido ao Regulador de Crescimento de Artr?podes Fluazuron. Disserta??o de Mestrado em Ci?ncias Veterin?rias. Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2007. 43f. MENDES, M.C.; SILVA, M.X.; BRACCO, J.E. Biochemical testto determine the resistance of two strains of the tick Boophilus microplus (Canestrini, 1887). Revista Brasileira de Parasitologia Veterin?ria, v.10, n.2, p. 61-65, 2001. MERZENDORFER H. The cellular basis of chitin synthesis in fungi and insects: common principles and differences. European Journal of Cell Biology, v.90, n.9, p. 759- 769, 2011. 66 MORAES, J.; GALINA, A.; ALVARENGA, P.H.; REZENDE, G.L.; MASUDA, A.; VAZ JR., I.S.; LOGULLO, C. Glucose metabolismo during embryogenesis of the hard tick Boophilus microplus. Comparative Biochemistry and Physiology, Part A, v. 146, p.528?533, 2007. MORANDO, A.; GELINSKI, J.N.L.M. Estudo preliminar do desenvolvimento embrion?rio in vitro de Rhipicephalus (Boophilus) microplus. Unoesc & Ci?ncia ? ACBS, v. 1, n. 1, p. 23-28, 2010. MORITA, A.; SOGA, K.; HOSON, T.; KAMISAKA, S.; NUMATA, H. Changes in mechanical properties of the cuticle and lipid accumulation in relation to adult diapause in the bean bug, Riptortusclavatus. Journal of Insect Physiology, v. 45, p.241?247, 1999. NATION, J.L. Insect physiology and biochemistry. Florida: CRC Press, 2002. NELSON, D.L.; COX, M.M. Princ?pios de Bioqu?mica de Lehninger. 5? ed. Porto Alegre: W.H. Freeman, 2011. 1100 p. NOVARTIS. Acatak? (Fluazuron) <http://www.ah.novartis.com/ products/en/acatak_cattle.shtml> Acessado em 05 de maio de 2012. OLIVEIRA, P.R.; CALLIGARIS,I.B.; ROMA, G.C.; BECHARA, G.H.; PIZANO, M.A; MATHIAS, M.I.C. Potential of the insect growth regulator, fluazuron, in the control of Rhipicephalus sanguineus nymphs (Latreille, 1806) (Acari: Ixodidae): Determination of the LD95 and LD50. Experimental Parasitology, 2012. OLIVEIRA, P.R.; CALLIGARIS, I.B.; NUNES, P.H.; BECHARA, G.H.; CAMARGO-MATHIAS, M.I. Fluazuron-induced morphological changes in Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) nymphs: An ultra-structural evaluation of the cuticle formation and digestive processes. Acta Tropica, v.133, p.45?55, 2014. PALLI, S.R.; RETNAKARAN, A. 1999. Molecular and Biochemical Aspects of chitin Synthesis Inhibition. p. 85-98. In: JOLLES, P.; MUZZARELLI, R.A.A (Eds.), Chitin and Chitinases. Birka user Verlag, Basel. PASAY, C.; ROTHWELL, J.; MOUNSEY, K.; KELLY, A.; HUTCHINSON, B.; MIEZLER, A.; MCCARTHY, J. An exploratory study to assess the activity of the acarine growth inhibitor, fluazuron, against Sarcoptes scabei infestation in pigs. Parasites & Vectors, v.40, n.5, 2012. PEREIRA, M.C.; LABRUNA, M.B.; SZAB?, M.P.J.; KLAFKE, G.M. Rhipicephalus (Boophilus) microplus. Biologia, controle e resist?ncia. 1? ed. S?o Paulo: MedVet, 2008, 169p. PINHEIRO J, GOMES E.M. A method for glycogen determination in mollusks. Brazilian Archives of Biology and Technology, v. 37, p.569-576, 1994. PONTES, E.G.; LEITE, P.; MAJEROWICZ, D.; ATELLA, G.C.; GONDIM, K.C. Dynamics of lipid accumulation by the fat body of Rhodnius prolixus: The involvement of lipophorin binding sites. Journal of Insect Physiology, v.54, p.790?797, 2008. 67 RAIA,V.A.Estudos bioqu?micos comparativos dos ovos de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) durante a oviposi??o. Tese de Doutorado em Ci?ncias Veterin?rias. Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011. 38f. RAIA, V.A.; FILHO, P.R.C.; PEDRENHO, A.R.; FLAUSINO, W.; FAMADAS, K.M. Quantitative and qualitative evaluation of the egg proteins of Rhipicephalus microplus and Rhipicephalus sanguineus (acari: ixodidae) during oviposition. Revista Brasileira de Medicina Veterin?ria, v.34, n.3; p.177-182, 2012. RECK J, KLAFKE GM, WEBSTER A, DALL?AGNOL B, SCHEFFER R, SOUZA UA.; CORASSINI, V.B.; VARGAS, R.; SANTOS, J.S.; MARTINS, J.R.S. First Report of fluazuron resistance in Rhipicephalus microplus: a field tick population resistant to six classes of acaricides. Veterinary Parasitology, v. 201, n.1-2, p. 128-136, 2014. REY, L. Parasitologia: parasitos e doen?as parasit?rias do homem nos tr?picos ocidentais. 4. ed. Rio de Janeiro: Guanabara Koogan, 2008. ROMA, G.C. FURQUIM, K.C.S.; BECHARA, G.R.; CAMARGO-MATHIAS, M.I. Permethrin-induced morphological changes in oocytes of Rhipicephalus sanguineus (Acari: Ixodidae) semi-engorged females. Food and Chemical Toxicology,v.48, p.825?830, 2010. RUIZ, J.I.; OCHOA, B. Quantification in the subnanomolar range of phospholipids and neutral lipids by monodimensional thin-layer chromatography and image analysis. Journal of Lipid Research, v. 38, p.1482?1489, 1997. SANTOS, A.P.; FURLONG, J. Competi??o intraespec?fica em Boophilus microplus. Ci?ncia Rural, v.32, n.6, p.1033-1038, 2002. SCIENTIFIC COMMITEE. Recommandations pour la mesure de la concentration catalytique de la lactate deshidrogenase dans le serum humain a 30?C, Annales Biologie Clinique, v.40, p.87-164, 1982. SEIXAS, A.; OLIVEIRA, P.; TERMIGNONIA, C.; LOGULLO, C.; MASUDA, A.; VAZ JR., I.S. Rhipicephalus (Boophilus) microplus embryo proteins as target for tick vaccine, Veterinary Immunology and Immunopathology, v.148, p.149? 156, 2012. SOBBHY, H.; AGGOUR, M. G.; SONENSHINE, D. E.; BURRIDGR, M. J. Cholesterol esters on the body surfaces of the camel tick, Hyalomma dromedarii (Koch, 1844) and the brown dog tick, Rhipicephalus sanguineus (Latreille, 1860). Experimental and Applied Acarology, v.18, p.265-280, 1994. SOCIEDAD ESPA?OLA DE QU?MICA CL?NICA, COMIT? CIENT?FICO, COMISI?N DE ENZIMAS. Met?do recomendado para la determinaci?n en rutina de la concentraci?n catal?tica de la alanina aminotransferasa en suero sangu?neo humano. Quimica Clinica, v.6, p.241-244, 1987. 68 SOCIEDAD ESPA?OLA DE QU?MICA CL?NICA, COMIT? CIENT?FICO. Comisi?n de Enzimas. M?todo recomendado para la determinaci?n en rutina de la concentraci?n catal?tica de lactato deshidrogenasa en suero sangu?neo humano, Qu?mica Cl?nica, v.8, p.57-61, 1989. SONENSHINE, D.E. The Biology of Ticks. 1. Ed. Oxford University Press, New York, USA, 1991. SOUZA, G.S. Avalia??o da atividade do novaluron, sobre Boophilus microplus (Canestrini) em bovinos de corte naturalmente infestados. Disserta??o de Mestrado Profissional em Gest?o, Pesquisa e Desenvolvimento em Tecnologia Farmac?utica. Universidade Cat?lica de Goi?s, Goi?nia, 2009. 44f. SVOBODA, J. A.; KAPLANIS, J. N.; ROBBINS, W. E.; THOMPSON, M. J. Recent developments in insect steroid metabolism. Annual Review of Entomology, v.20, p.205-220, 1975. TAN, YA.; XIAO, LB.; SUN, Y.; ZHAO, J.; BAI, LX. Sublethal effects of the chitin synthesis inhibitor, hexaflumuron, in the cotton mirid bug, Apoliguslucorum (Meyer-D?r). Pesticide Biochemistry and Physiology, n.111, p. 43-50, 2014. TATCHELL, R.J.; SCHUNTNER, C.A. Glucose metabolism in the cattle tick Boophilus microplus. Journal of Insect Physiology, v.18, n. 2, p. 283?288, 1972. TRINDER, P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, v.6, p.24-27, 1969. TUNHOLI, V.M.; LUSTRINO, D.; TUNHOLI-ALVES, V.M.; MELLO-SILVA, C.C.C.; MALDONADO JR.; A.; PINHEIRO, J.; RODRIGUES, M.L.A. Biochemical profile of Biomphalaria glabrata (Mollusca: Gastropoda) after infection by Echinostoma paraensei (Trematoda: Echinostomatidae). Parasitology Research, v.109, p.885?891, 2011. TUNHOLI-ALVES, V.M., TUNHOLI, V.M., CASTRO, R.N., SANT'ANA, L.D.O., SANTOS-AMARAL, L., OLIVEIRA, A.P.M., GARCIA, J., THIENGO, S.C., PINHEIRO, J. AND MALDONADO JR, A. Activation of anaerobic metabolism in Biomphalaria glabrata (Mollusca: Gastropoda) experimentally infected by Angiostrongylus cantonensis (Nematoda, Metastrongylidae) by high-performance liquid chromatography, Parasitology International, v.63, p.64?68, 2014. UNGER, T.A. Fluazuron. In: Pesticide Synthesis Handbook, Noyes Publications. Nova Jersey, EUA. p.231-232, 1996. VIEIRA, V.P.C. Efic?cia do regulador de Crescimento de Artr?podes Fluazuron no Controle da pulga Ctenocephalidesfelisfelis (Bouch?, 1835) (Siphonaptera: Pulicidae) em C?es. Disserta??o de Mestrado em Ci?ncias Veterin?rias. Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2009. 45f. 69 WANG, Y.; ZHAO, S.; SHI, L.; XU, ZHIFENG; HE, L. Resistance selection and biochemical mechanism of resistance against cyflumetofen in Tetranychus cinnabarinus (Boisduval). Pesticide Biochemistry and Physiology, n.111, p. 24-30, 2014. WHO, 1997. Toxicological evaluation of certain veterinary drug residues in food. International Programme on Chemical Safety. World Health Organization <http://www.inchem.org/documents/jecfa/jecmono/v39je09.htm> Geneva: 1997. Acessado em: 05 de maio de 2012. WILLADSEN, P.; KEMP, D.H.; MCKENNA, R.V. Blood meal ingestion and utilization as a component of host specificity in the tick, Boophilus microplus. Zeitschrift for Parasitenkunde, n. 70, p.415-420, 1984. ZIMMER, K.R.; MACEDO, A.J.; NICASTRO, G.G.; BALDINI, R.L.; TERMIGNONI, C. Egg wax from the cattle tick Rhipicephalus (Boophilus) microplus inhibits Pseudomonas aeruginosa biofilm.TicksandTick-borne Diseases, v.4, p.366? 376, 2013. |
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