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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

An?lise do crescimento, diferencia??o celular e perfil isoenzim?tico de um isolado de tripanosomat?deo obtido de Megaselia scalaris Loew, 1866 (D?ptera, Phoridae) / Analysis of growth, cell differentiation and isoenzyme profile of an isolated trypanosomatid obtained Megaselia scalaris Loew, 1866 (Diptera, Phoridae)

Lima , Nathanielly Rocha Casado de 30 March 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-28T15:23:40Z No. of bitstreams: 1 2011 - Nathanielly Rocha Casado de Lima.pdf: 972650 bytes, checksum: 29dba077fa4bb5ed1cb4977f2f174d44 (MD5) / Made available in DSpace on 2016-09-28T15:23:40Z (GMT). No. of bitstreams: 1 2011 - Nathanielly Rocha Casado de Lima.pdf: 972650 bytes, checksum: 29dba077fa4bb5ed1cb4977f2f174d44 (MD5) Previous issue date: 2011-03-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa. In this study, an isolate was obtained by feces culturing of the intestinal tract of Megaselia scalaris Loew, 1866 (Diptera, Phoridae). The original isolate and its clones (obtained by flow cytometry) were deposited in the ?Trypanosomatids Collection of the Instituto Oswaldo Cruz. These clones were characterized by different approaches I in comparison with the reference species of different genera. Its growth has been studied in LIT medium (with 10 or 20% fetal bovine serum) modified according to the requirement. The number of cells per microliter was estimated at 24 h intervals between 48-144 h. Giemsa-stained smears (after acid hydrolysis) were prepared in order to study cell differentiation and morphology of the forms. The isozyme analysis was performed using the following systems: GPI, PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI and HK. Data from this analysis were processed and analyzed numerically using the computacional Jaccard coefficient and UPGMA algorighm. The result set of this study suggest that: (1) The strain of M. scalaris showed 70% similarity with Herpetomonas megaseliae, (2) This isolate showed the exclusive presence of promastigotes in any analysis of the growth curve as expected for the genus Leptomonas, however the forms exhibited high polymorphism. Additional studies using molecular tools will be realized / A fam?lia Trypanosomatidae inclui parasitas de uma grande variedade de vertebrados, invertebrados (principalmente insetos), plantas e algumas esp?cies de protozo?rios. No presente trabalho, um isolado foi obtido por coprocultivo de trato intestinal de Megaselia scalaris Loew, 1866 (Diptera, Phoridae). O isolado original e seus clones (obtidos por citometria de fluxo) foram depositados na "Cole??o de Tripanosomat?deos do Instituto Oswaldo Cruz". Estes clones foram caracterizados por diversas abordagens em compara??o com esp?cies de refer?ncia de diferentes g?neros. Seu crescimento foi estudado em meio LIT (com 10 ou 20% de soro fetal bovino) modificado, de acordo com o n?vel de exig?ncia. O n?mero de c?lulas por microlitro foi estimado em intervalos de 24 h, entre 48-144 h. Esfrega?os corados pelo Giemsa (ap?s hidr?lise ?cida) foram preparados visando estudos de diferencia??o celular e morfometria das formas encontradas. A an?lise de isoenzimas foi realizada utilizando-se os seguintes sistemas: GPI , PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI e HK. Os dados desta an?lise foram processados numericamente e submetidos ? an?lise computacional utilizando-se o coeficiente de Jaccard e o algoritmo UPGMA. O conjunto de resultados deste trabalho sugere que: (1) O isolado de M. scalaris apresentou similaridade de 70% com Herptomonas megaseliae; (2) Esse isolado revelou a presen?a exclusiva de promastigotas em toda an?lise da curva de crescimento, como esperado para o g?nero Leptomonas, entretanto as formas exibiram grande polimorfismo. Estudos complementares utilizando ferramentas moleculares ser?o realizados.
2

An?lise transcrit?mica do intestino de f?meas ingurgitadas de Ornithodoros mimon (Acari: Argasidae) / Gut transcriptome analysis on engorged females of Ornithodoros mimon (Acari: Argasidae).

Landulfo, Gabriel Alves 26 February 2016 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-06T11:37:49Z No. of bitstreams: 1 2016 - Gabriel Alves Landulfo.pdf: 2085230 bytes, checksum: a7f7c364b23211ad81d1168cb1f6db85 (MD5) / Made available in DSpace on 2017-01-06T11:37:49Z (GMT). No. of bitstreams: 1 2016 - Gabriel Alves Landulfo.pdf: 2085230 bytes, checksum: a7f7c364b23211ad81d1168cb1f6db85 (MD5) Previous issue date: 2016-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also aggressive towards humans. It inhabits some countries in the Neotropical region. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to ascertain the main molecules expressed in the gut of argasid ticks after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon. Sixty females were fed and dissected to extract the gut tissue. The transcriptome was obtained through pyrosequencing and the de novo assembly method on mRNA of the gut tissue. We identified 2,235 contigs, of which 1,729 matched database sequences, while 506 did not present any hits. The transcripts were annotated and grouped according to their biological function. Catalytic, binding and transporter activity were the most representative functions, accounting for 780, 709 and 106 contigs, respectively. The transcripts were classified into 31 categories, using both bioinformatics and data curation practices. The most representative categories were, respectively, unknown, catalytic activity and transporter channels. One hundred and three (103) digestives transcritps associated to digestion of proteins (67), carbohydrates (19) and lipid (17) were identified in the transcriptome analysis. Peptidases associated with hemoglobin digestion, such as serine, cysteine, aspartic protease and metalloenzymes, were identified in the gut of the engorged females. Genes associated with transport (hemelipoglycoprotein) and storage (ferritin) of nutrients resulting from hemoglobin digestion, such as heme, were also found in the digestive tract. The presence of a cathepsin O-like cysteine peptidase was recorded in ticks, for the first time. Two thousand and two hundred thirteen (2213) transcripts were deposited to the Transcriptome Shotgun Assembly (TSA) portal of the NCBI.The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. Genes for cathepsin L in O. mimon appear to have diverged from other more common recent ancestors. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on different datasets, such as mitochondrial genome and nuclear rRNA sequences. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity / Ornithodoros mimon ? um carrapato argas?deo parasita de quir?pteras, aves e marsupiais, al?m de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de mol?culas vitais no processo digest?o e na rela??o parasito-hospedeiro, al?m de fornecer tamb?m informa??es sobre a evolu??o dos artr?podes hemat?fagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais mol?culas expressas no intestino de uma esp?cie de carrapato argas?deo ap?s o repasto sangu?neo, atrav?s de uma an?lise transcrit?mica do intestino de f?meas ingurgitadas de O. mimon. Sessenta f?meas foram alimentadas e dissecadas para coleta o tecido intestinal. O RNAm da amostra do intestino foi extra?do, purificado e quantificado. Esse serviu de molde para s?ntese do cDNA, que foi utilizado no pirosequenciamento. O transcritoma foi obtido atrav?s do m?todo de montagem de novo do cDNA do tecido intestinal. Identificou-se 2235 sequ?ncias consensos (contigs) ou transcritos, dos quais 1729 apresentaram similaridade (hit) com sequ?ncias dos bancos de dados, enquanto que 506 n?o tiveram nenhuma similaridade. Os transcritos foram anotados e agrupados conforme as fun??es biol?gicas atribu?das as eles no processo de anota??o g?nica. Atividade catal?tica, ades?o e transporte foram as fun??es mais representativas com 780, 709 e 106 transcritos, respectivamente. Em uma an?lise n?o automatizada, os transcritos foram subcategorizados em 31 categorias. As categorias mais representativas foram desconhecido, atividade catal?tica e transportadores-canais. Identificamos 103 transcritos digestivos associados ? digest??o de prote?nas (67), carboidratos (19) e lip?dios (17). Proteinases das classes serino, ciste?ne, asp?rtica e metalo representaram as enzimas atuantes na digest?o intracelular do constituinte prote?co do repasto sangu?neo. Genes associados com o transporte (hemelipoglicoprote?na) e estocagem (ferritina) dos nutrientes resultantes da digest?o foram encontrados bem expressos no trato digestivo. Registrou-se pela primeira vez a presen?a de uma ciste?na peptidase do tipo catepsina O em carrapatos. Foram depositados no banco de dados g?nico p?blico 2213 transcritos de O. mimon. A an?lise filogen?tica das peptidases revelou que a maioria das proteinases de O. mimon ? pr?xima aos genes codificadores de proteinases de carrapatos. Transcritos de catepsinas L de O. mimon parecem ter divergido de ancestrais recentes diferentes. A infer?ncia filogen?tica baseada em conjunto de dados transcritos hom?logos tem uma resolu??o topol?gica similar a de outros conjuntos de dados, como genoma mitocondrial e sequ?ncias nuclear de RNA riboss?mico (rRNA). Os achados obtidos no presente estudo podem contribuir para compreens?o dos importantes processos dos carrapatos argas?deos, como digest?o, nutri??o e imunidade, al?m de fornecer informa??es sobre a filogenia dos carrapatos.
3

Diagn?stico de parasitos de peixes Perciformes: uma esp?cie de Ergasilus (Copepoda: Ergasilidae) e duas esp?cies de Encotyllabe (Monogenea: Capsalidae) / Parasites diagnostic fish Perciformes: one species of Ergasilus (Copepoda: Ergasilidae) and two species of Encotyllabe (Monogenea: Capsalidae).

Taborda, Naraiana Lopes 22 December 2015 (has links)
Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-07-06T13:19:39Z No. of bitstreams: 1 2015 - Naraiana Lopes Taborda.pdf: 2569264 bytes, checksum: dd82638f045c3152324e7f8757f3a526 (MD5) / Made available in DSpace on 2017-07-06T13:19:39Z (GMT). No. of bitstreams: 1 2015 - Naraiana Lopes Taborda.pdf: 2569264 bytes, checksum: dd82638f045c3152324e7f8757f3a526 (MD5) Previous issue date: 2015-12-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The current work aimed to increase the knowledge of biodiversity of parasite of fishes, unknown to some groups. In Chapter 1 the aim was described the new species ergasilid copepod Ergasilus von Nordmann, 1832, found on the gills of two cichlid fishes, Geophagus argyrostictus Kullander, 1991 (type host) and G. altifrons Heckel, 1840, caught in the Xingu River around Altamira, State of Par?, Brazil. The new species is the first record of genus Ergasilus found on fishes G. argyrostictus e G. altifrons. In Chapter 2, species of Encotyllabe Perciformes host were analyzed by molecular study, with analysis of cytochrome c oxidase I gene (COI) and morphological study. These data identify two new species, a parasite of Pagrus pagrus (Sparidae) and other of Orthopristis ruber (Haemulidae), and addition of the sequence mitochondrial DNA to E. souzalimae Carvalho e Luque, 2012. / O presente trabalho teve como objetivo ampliar os conhecimentos sobre a biodiversidade de parasitos de peixes, visto o escasso conhecimento para alguns grupos. No cap?tulo 1 objetivamos a descri??o de uma nova esp?cie do cop?pode parasito Ergasilus von Nordmann, 1832, encontrado parasitando as br?nquias de duas esp?cies de cicl?deos, Geophagus argyrostictus Kullander, 1991 (esp?cie tipo) e G. altifrons Heckel, 1840, capturados no Rio Xingu em torno de Altamira, Estado do Par?, Brasil. A nova esp?cie ? o primeiro registro do g?nero Ergasilus encontrado em peixes G. argyrostictus e G. altifrons. No cap?tulo 2, esp?cies de Encotyllabe encontradas em hospedeiros Perciformes foram analisadas atrav?s do estudo molecular, com an?lise do gene citocromo c oxidase I (COI) e morfol?gico. Estes dados identificam duas esp?cies novas, uma parasita de Pagrus pagrus (Sparidae) e outra de Orthopristis ruber (Haemulidae) e a adi??o da sequ?ncia de DNA mitocondrial para E. souzalimae Carvalho e Luque, 2012.
4

Efic?cia da associa??o de abamectina com fluazuron no controle de Rhipicephalus microplus, Dermatobia hominis e nemat?ides gastrointestinais em bovinos / Efficacy of abamectin with fluazuron association in control of Rhipicephalus microplus, Dermatobia hominis and gastrointestinal nematodes in cattle

Rocha, Cristiane Nunes Coelho da 21 March 2016 (has links)
Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-16T12:11:58Z No. of bitstreams: 1 2016 - Cristiane Nunes Coelho da Rocha.pdf: 2134676 bytes, checksum: 7d111f5f6a4f012cde150c537673a52f (MD5) / Made available in DSpace on 2017-10-16T12:11:58Z (GMT). No. of bitstreams: 1 2016 - Cristiane Nunes Coelho da Rocha.pdf: 2134676 bytes, checksum: 7d111f5f6a4f012cde150c537673a52f (MD5) Previous issue date: 2016-03-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The purpose of the research was to evaluate the efficacy of association abamectin 0.6% with fluazuron 3.0% in control of Rhipicephalus microplus, Dermatobia hominis and gastrointestinal nematodes in cattle. For this, there were five tests in which the control group animals received no treatment, while animals in the treated group received the formulation within the association in a single dose of 600 mcg of abamectin and 3 mg of fluazuron per Kg of body weight by route ?pour-on?. As to acaricide efficacy, two tests were conducted, one using artificially infested cattle and other naturally infested by Rhipicephalus microplus. In the same manner, a test was conducted to evaluate the efficacy of the product in test front of Dermatobia hominis naturally infested cattle. And two tests anthelmintic efficacy in infected cattle naturally and artificially by gastrointestinal nematodes. The mean of the carrapaticide efficacy for cattle artificially infested with R. microplus over the 23 days after treatment was 87,40%. The preventive effectiveness results of 98,12%; 98,68%; 98,36%; 97,88%; 96,76%; 96,45%; 96,84%; 97,03%; 95,36% and 87,07% respectively for the experimental days +7, +14, +21, +28, +35, +42, +49, +56, +63, +70. The acaricide effectiveness for naturally infested cattle by R. microplus was 96,30%; 96,90%; 95,98%; 96,83%; 98,32%; 96,12%; 96,15%; 98,8%; 97,18%, 99,12%; 97,18%; 98,04% and 77,50%, respectively for the experimental days +7, +14, +21, +28, +35, +42, +49, +56, +63, +70, +77, +84 and +91 for the treated group. The efficacy of the test product to D. hominis was 90,70% and 96,84% for days +7 and +14. The values of anthelmintic efficacy in naturally infected cattle with gastrointestinal nematodes was 92,17%; 89,32%; 87,38% respectively for the experimental days +7, +14, +21 for the treated group. For cattle artificially infected by gastrointestinal nematodes the efficacy was 92,59%; 97,62%; 87,82%; 86,96%; 96,00% respectively for Haemonchus placei, Trichostrongylus axei, Cooperia punctata, Cooperia pectinata and Oesophagostomum radiatum. The association abamectin 0.6% with fluazuron 3.0% was effective in artificially infested cattle and naturally infested by R. microplus, as well as demonstrates efficacy for D. hominis. The test product showed efficacy also in the reduction the eggs counts per gram of feces (EPG) in cattle naturally infected with gastrointestinal nematodes, as well as shown to be effective front of artificial infections with H. placei, T. axei and O. radiatum and has presented moderately effective against C. pectinata and C. punctata / O objetivo da pesquisa foi avaliar a efic?cia da associa??o de abamectina 0.6% com fluazuron 3.0% controle de Rhipicephalus microplus, Dermatobia hominis e nemat?ides gastrointestinais em bovinos. Para isso, foram realizados cinco ensaios, nos quais os animais do grupo controle n?o receberam tratamento, enquanto que os animais do grupo tratado receberam a formula??o da associa??o na dose ?nica de 600 mcg de abamectina com 3 mg de fluazuron por Kg de peso corporal, por meio de via ?pour-on?. Quanto ? atividade carrapaticida, foram conduzidos dois ensaios, um utilizando bovinos artificialmente infestados e outro naturalmente infestados por Rhipicephalus microplus. Da mesma forma, foi realizado um ensaio para avaliar a efic?cia do produto em teste frente a Dermatobia hominis em bovinos infestados naturalmente e dois ensaios de efic?cia anti-helm?ntica em bovinos naturalmente e artificialmente infectados por nemat?ides gastrointestinais. A m?dia da efic?cia carrapaticida para bovinos artificialmente infestados por R. microplus ao longo dos 23 dias ap?s o tratamento foi de 87,40%. O resultado da efic?cia preventiva foi de 98,12%; 98,68%; 98,36%; 97,88%; 96,76%; 96,45%; 96,84%; 97,03%; 95,36%; e 87,07% respectivamente para os dias experimentais +7, +14, +21, +28, +35, +42, +49, +56, +63, +70. A efic?cia carrapaticida para bovinos naturalmente infestados por R. microplus foi de 96,30%; 96,90%; 95,98%; 96,83%; 98,32%; 96,12%; 96,15%; 98,8%; 97,18%, 99,12%; 97,18%; 98,04% e 77,50%, respectivamente para os dias experimentais +7, +14, +21, +28, +35, +42, +49, +56, +63, +70, +77, +84 e +91 para o grupo tratado. A efic?cia do produto em teste para D. hominis foi de 90,70% e 96,84%, para os dias +7 e +14. Os valores de efic?cia anti-helm?ntica em bovinos naturalmente infectados por nemat?ides gastrointestinais foi de 92,17%; 89,32%; 87,38% respectivamente para os dias experimentais +7, +14, +21 para o grupo tratado. Para bovinos artificialmente infectados por nemat?ides gastrointestinais, a efic?cia foi de 92,59%; 97,62%; 87,82%; 86,96%; 96,00% respectivamente para Haemonchus placei, Trichostrongylus axei, Cooperia punctata, Cooperia pectinata e Oesophagostomum radiatum. A associa??o de abamectina 0.6% com fluazuron 3.0% demonstrou ser eficaz em bovinos artificialmente e naturalmente infestados por R. microplus, assim como apresentou efic?cia para D. hominis. O produto em teste apresentou efic?cia tamb?m na redu??o de ovos por grama de fezes (OPG) em bovinos naturalmente infectados por nemat?ides gastrointestinais, assim como, demonstrou ser efetivo frente a infec??es artificiais por H. placei, T. axei e O. radiatum, e apresentou-se moderadamente efetivo frente a C. pectinata e C. punctata.
5

Defeitos de esmalte em dentes dec?duos: preval?ncia e fatores associados. / Enamel defects in the primary teeth: prevalence and associated factors

Faria, Patr?cia Corr?a de January 2011 (has links)
Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:38:49Z (GMT) No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:38:49Z (GMT). No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Os estudos sobre o desenvolvimento de defeitos de esmalte e a associa??o com as condi??es natais e p?s-natais ainda s?o escassos. Assim, este estudo teve como objetivo avaliar a preval?ncia de defeitos de esmalte em dentes dec?duos e verificar se prematuridade, peso ao nascimento e vari?veis sociodemogr?ficas podem estar associados a tais defeitos. Foi realizado um estudo transversal com 381 crian?as com idade entre 3 e 5 anos. Os dados foram coletados atrav?s de exame cl?nico e entrevistas com os pais durante Campanha Nacional de Vacina??o. O diagn?stico dos defeitos de esmalte foi realizado utilizando-se o Developmental Defects of Enamel Index (DDE Index). Atrav?s de entrevista foram coletadas informa??es sobre aspectos sociodemogr?ficos, gesta??o, peso ao nascimento, prematuridade da crian?a e amamenta??o. A an?lise dos dados foi realizada utilizando-se o programa SPSS for Windows 17.0 e incluiu a distribui??o de frequ?ncia, testes qui-quadrado e Exato de Fisher e regress?o de Poisson. A preval?ncia de defeito de esmalte foi de 29,9% e a opacidade demarcada foi o tipo de defeito mais frequente. Crian?as com peso muito baixo ao nascimento apresentam mais chance de desenvolver defeitos de esmalte [RP (IC95%) = 2,7 (1,66-4,61)]. A prematuridade e as vari?veis sociodemogr?ficas n?o foram fatores associados aos defeitos de esmalte. Crian?as com peso muito baixo ao nascimento apresentaram maior preval?ncia de defeitos de esmalte nos dentes dec?duos. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT Studies regarding the development of enamel defects and association with natal and postnatal conditions are scarce. Thus, the aim of this study was to evaluate the prevalence of enamel defects in primary teeth and determine whether prematurity, birth weight and sociodemographic variables may be associated with such defects. This was a cross-sectional study with 381 children aged between 3 and 5 years. Data were collected through clinical examination and interviews with parents during the National Immunization Day. The diagnosis of enamel defects was performed using the Development Defects of Enamel Index (DDE Index). During interviews, information were collected on sociodemographic aspects, pregnancy, birth weight, child prematurity and breastfeeding. Statistical analysis was performed using the SPSS software for Windows 17.0 and involved descriptive analysis, the Fisher?s test, qui-square test and Poisson regression. The prevalence of enamel defects was 29.9% and demarcated opacity was the most frequent type of defect. Children with low birth weight presented greater prevalence of enamel defects [PR (CI 95%) = 2,7 (1.66-4.61)]. Prematurity and the sociodemographic variables were not factors associated with enamel defects. In this study, children with very low birth weight had higher prevalence of enamel defects in deciduous teeth.
6

Efeito pr?-operat?rio da dexametasona e da metilprednisolona no controle da dor, edema e trismo ap?s cirurgia de terceiro molar: um ensaio cl?nico randomizado, triplo-cego, boca dividida. / Preemptive effect of dexamethasone and methilprednisolone on pain, swelling and trismus?after third molar surgery: a split-mouth randomized triple-blind clinical trial

Alc?ntara, Carlos Eduardo Pinto de January 2012 (has links)
Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:42Z No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T11:09:38Z (GMT) No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T11:09:38Z (GMT). No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / O objetivo do presente estudo foi realizar uma avalia??o comparativa do efeito da dexametasona e da metilprednisolona no controle da dor, edema e limita??o da abertura bucal ap?s a extra??o de terceiros molares impactados. Dezesseis pacientes saud?veis (3 homens e 13 mulheres) com m?dia de 20,3 (DP= 1,25) anos de idade foram submetidos ? extra??o bilateral dos terceiros molares com intervalo de tr?s a quatro semanas entre as duas cirurgias. Uma hora antes de cada procedimento cir?rgico foi administrada em dose ?nica, por via oral, dexametasona 8 mg ou metilprednisolona 40 mg. Durante os intervalos p?s-operat?rios de 24, 48 e 72 horas e sete dias, o edema linear foi determinado utilizando medidas na face e o trismo atrav?s da abertura bucal m?xima. A dor p?s-operat?ria foi auto-registrada pelo paciente utilizando escala anal?gica visual durante as 72 horas iniciais, em intervalos de oito horas. A an?lise estat?stica envolveu an?lise descritiva, teste McNemar, Teste T pareado e Wilcoxon (p<0,05). A dexametasona apresentou melhor controle do edema em todos os per?odos p?s-operat?rios (p <0,02) e da abertura bucal dois dias ap?s a cirurgia (p = 0,029). Com rela??o ? dor n?o foi observada diferen?a estatisticamente significativa entre os medicamentos. A administra??o pr?-perat?ria de 8 mg de dexametasona apresentou melhor controle do edema e da limita??o da abertura bucal do que a metilprednisolona na dose de 40 mg, muito embora os medicamentos n?o diferiram no controle da dor. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The?aim of this study?was to make a comparative assessment of the effect of?dexamethasone and methylprednisolone?to control pain, swelling?and limitation?of mouth opening after extraction of?impacted third molars.?Sixteen healthy patients (3 men and 13 women)?with a mean age?of 20.3 (SD = 1,25) years underwent bilateral extraction?of?third molars with na interval of three to four weeks between the two surgeries. One hour?before each?surgical procedure?a single oral dose?of?dexamethasone 8?mg or methylprednisolone?40?mg was administered.?During the postoperative?intervals?of 24,?48?and 72?hours and?seven days, the swelling was determined using?linear?measures?in the?face and trismus?by?maximum mouth opening. The?postoperative pain?was self-recorded?by the patient using?visual analogue scale?in?72 hours with an interval of eight hours. Statistical analysis involved descriptive analysis, McNemar test, Wilcoxon?and paired T test (p?<0.05). Dexamethasone showed better control of swelling in all?postoperative periods (p?<0.02) and of mouth opening two days after surgery (p = 0.029). Regarding pain there was no statistically significant difference between the drugs. Preemptive dexamethasone?8 mg showed better control of swelling?and limitation?of mouth opening than methylprednisolone 40 mg, but there was no difference?in pain control.
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Diversidade da Calliphoridae em manguezal e a associa??o com Megaselia scalaris (Loew) (Diptera: Phoridae), Itabora?, RJ, Brasil / Diversity of Calliphoridae in mangrove swamp and the association with Megaselia scalaris (Loew) (Diptera: Phoridae), Itabora?, RJ, Brazil

SILVA, Jos? Antonio Batista da 08 December 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-08T12:13:31Z No. of bitstreams: 1 2011 - Jos? Antonio Batista da Silva.pdf: 1901739 bytes, checksum: bf67996decc8c7cef031a8ce05d6f464 (MD5) / Made available in DSpace on 2016-09-08T12:13:31Z (GMT). No. of bitstreams: 1 2011 - Jos? Antonio Batista da Silva.pdf: 1901739 bytes, checksum: bf67996decc8c7cef031a8ce05d6f464 (MD5) Previous issue date: 2011-12-08 / This work was carried out from August 2007 to July 2011, in Itabora?, RJ, Brazil, and aimed to identify the Calliphoridae species exist in a mangrove swamp, verifying which are the predominant species, highlighting the proportions of males and females, and also analyzing the influence of abiotic factors such as temperature, humidity and moon phases, and also providing an analysis of the abundance, richness, diversity and similarity between the periods of sampling, ecological relationships between species of the same family and the ecological relationship between Calliphoridae and Phoridae parasitoids in Guapi-Mirim Environmental Protection Area, (Guapi-Mirim EPA) in the same city. Semiweekly collections were carried out over 48 months (96 collections). The traps were suspended at a height of 1.20 m above the ground for a period of 48 hours for each collection. To trap and collect the flies, four plastic traps (35 cm x 15 cm) were placed 100m apart each other in the studied area. The bait used was based on fish (sardine) in decomposition. The insects caught were killed by asphyxiation with 70% ethanol inside the trap. After asphyxiation all specimens were put into plastic pots containing 70% ethanol. The specimens were taken to the Laborat?rio de Transmissores de Leishmanioses (Setor de Entomologia M?dica e Forense) - IOC/FIOCRUZ, RJ, where they were separated by the collection day, counted and then identified using a stereoscopic microscope and the dichotomous keys for families and species. The abundance of Calliphoridae flies was statistically analyzed using the Statistica 7.1 program (StatSoft 2005) for the non-parametric test Kruskal-Wallis one way ANOVA (p< 0.05). Also the Kendall Tau Correlation test, Mann-Whitney (significance of 95%; p<0.05) and chi-square test (?2) were used to analyze. The Shannon-Wiener diversity index, Bray-Curtis similarity and Euclidean Distance were used. A total of 4,531flies were collected. These flies belonged to ten (10) species of the Calliphoridae family: Chrysomya megacephala (Fabricius, 1794) (86.40%), Chrysomya albiceps (Wiedemann,1819) (5.72%), Cochliomyia macellaria (Fabricius, 1775) (4.94%), Chrysomya putoria (Wiedemann, 1818) (2.10%), Lucilia eximia (Wiedemann, 1819) (0.30%), Cochliomyia hominivorax (Cocquerel, 1858) (0.22%), Chloroprocta idioidea (Robineau-Desvoidy, 1930) (0.20%), Hemilucilia segmentaria (Fabricius, 1805) (0.04%), Hemilucilia semidiaphana (Rondani, 1850) (0.04%), Lucilia cuprina (Wiedemann,1830) (0.04%). The species C. megacephala was the one that presented the greatest abundance. Spring was the preferred season for all species; temperatures between 30.5 and 32.40C and relative humidity between 56.8 and 61.7% were considered those where there was a frenzy of oviposition; the full and new moons were the phases during which there was a higher occurrence of flies; Megaselia scalaris occurred more frequently in the most abundant Calliphoridae species in the same season of the year thus allowing greater dispersion of eggs; Calliphoridae species had a higher rate survival with increased food resources resulting from the polluting activity / Este trabalho foi realizado entre agosto de 2007 e julho de 2011, no Munic?pio de Itabora?, RJ, Brasil, e teve como objetivo identificar as esp?cies de Calliphoridae existentes em uma ?rea de mangue, quantificar as predominantes, destacando as propor??es de machos e f?meas, e tamb?m analisar a influ?ncia de fatores abi?ticos, tais como temperatura, umidade do ar e fases lunares; e ainda relacionar a abund?ncia, a riqueza, a diversidade e a similaridade entre os per?odos de coletas, rela??es ecol?gicas entre as esp?cies da entomofauna da mesma fam?lia e a rela??o ecol?gica entre Calliphoridae e Phoridae parasit?ides dentro da ?rea de Prote??o Ambiental de Guapi-Mirim (APA de Guapi-Mirim), em Itabora?. Durante 48 meses, foram realizadas 96 coletas, uma a cada 15 dias. Em todas as capturas, foram utilizadas armadilhas confeccionadas em recipiente pl?stico com 35 cm de altura e 15 cm de di?metro. Cada uma das quatro armadilhas foi suspensa a uma altura de 1,20 m do solo contendo 100g de isca de peixe (sardinha) em decomposi??o por um per?odo de 48 horas. Ap?s cada captura, todos os esp?cimes foram mortos no interior das armadilhas por asfixia utilizando etanol 70%. Em seguida todos os indiv?duos foram acondicionados em potes pl?sticos, contendo etanol a 70% e encaminhados ao Laborat?rio de Transmissores de Leishmanioses (Setor de Entomologia M?dica e Forense), IOC-FIOCRUZ, RJ, em seguida todos os esp?cimes foram separados por dia de coleta, identificadas e quantificadas. Para tal procedimento utilizou-se um microsc?pio estereosc?pico e chaves dicot?micas para a identifica??o da fam?lia e das esp?cies. As an?lises estat?sticas foram feitas utilizando o programa estat?stico Statistica 7.1 (STATSOFT, 2005), atrav?s do teste de Kruskal- Wallis one way ANOVA, teste de qui- quadrado (?2), Mann-Whitney e correla??o de Kendall Tau (p<0,05). Foram utilizados os ?ndices de diversidade de Shannon-Wiener e similaridade de Bray-Curtis, assim como a Dist?ncia Euclidiana. Foram capturadas 4531 moscas pertencentes a dez (10) esp?cies da fam?lia Calliphoridae: Chrysomya megacephala (Fabricius, 1794) (86,40%), Chrysomya albiceps (Wiedemann,1819) (5,72%), Cochliomyia macellaria (Fabricius, 1775) (4,94%), Chrysomya putoria (Wiedemann, 1818) (2,10%), Lucilia eximia (Wiedemann, 1819) (0,30%), Cochliomyia hominivorax (Cocquerel, 1858) (0,22%), Chloroprocta idioidea (Robineau-Desvoidy, 1930) (0,20%), Hemilucilia segmentaria (Fabricius, 1805) (0,04%), Hemilucilia semidiaphana (Rondani, 1850) (0,04%), Lucilia cuprina (Wiedemann,1830) (0,04%). A esp?cie C. megacephala foi aquela que apresentou a maior abund?ncia, a primavera foi a esta??o do ano em que todas as esp?cies mostraram maior prefer?ncia; as temperaturas compreendidas entre 30,5 e 32,40C e a umidade relativa do ar entre 56,8 e 61,7% foram consideradas aquelas onde houve frenesi de oviposi??o; nas luas cheias e novas houve maior ocorr?ncia de moscas capturadas; Megaselia scalaris se utilizou de esp?cies abundantes nas mesmas esta??es do anos em que ela ocorreu com maior frequ?ncia, possibilitando assim maior dispers?o de ovos; a maior disponibilidade de recursos alimentares resultantes da atividade poluidora tempor?ria ofereceram uma melhor condi??o de sobreviv?ncia para as esp?cies os Calliphoridae.
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Estudos bioqu?micos comparativos dos ovos de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) durante a oviposi??o / Biochemical Analysis of eggs of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) during the Oviposition.

Raia, Vanessa de Almeida 28 January 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-09T11:45:13Z No. of bitstreams: 1 2011 - Vanessa de Almeida Raia.pdf: 1415435 bytes, checksum: 6553cdee2bd5a5f2a363cb3cd11f812d (MD5) / Made available in DSpace on 2016-09-09T11:45:13Z (GMT). No. of bitstreams: 1 2011 - Vanessa de Almeida Raia.pdf: 1415435 bytes, checksum: 6553cdee2bd5a5f2a363cb3cd11f812d (MD5) Previous issue date: 2011-01-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / To fill some gaps about the intrinsic mechanisms of the biology of oviposition of R. (B.) microplus, we evaluated the concentration of lipids and carbohydrates, as well as the variability of lipid in eggs per day of posture. For this, engorged females weighing between 151 and 360 mg were placed in controlled environment (27 ? 1 ? C, 80 ? 5% RH, darkness), and after beginning of laying, three samples of the eggs pool were daily collected, packed and preserved at -20 ? C. Samples 1 and 2, weighing 50 mg each, were used to quantitate lipids and glucose, and to evaluate the lipid profile, respectively; and the third sample, containing 1 g of eggs, was used to quantitate glycogen. The determination of total lipids was performed using phospho-vanillin reagent, while the concentrations of cholesterol, triglycerides and glucose were obtained by colorimetric determination using endpoint enzymatic kit. The level of glycogen was performed using the acid 3,5 dinitrosalicylic reactive . The results were analyzed by nonparametric Kruskal-Wallis and the relationship between the concentrations of substrates and the days of laying was assessed by polynomial regression. There was no significant difference in the concentrations of these substrates in eggs from different days of oviposition, as there was no relationship of these concentrations with the days of laying. After lipid extraction, the samples were performed in thin layer chromatography, high-performance (HPTLC) for neutral lipids and phospholipids. Then, densitometry was performed using the Image Master Total Lab and the daily percentage of each lipid in the samples was calculated. Among the neutral lipids were identified the hydrocarbons, esterified cholesterol, triglycerides, fatty acids, free cholesterol and diacylglycerol. Of these, the cholesterol ester showed the highest variation, it was not detected in eggs at the fifth day of oviposition. Among the phospholipids were detected the phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin fosfatidilinusitol. This latter was absent in eggs of the seventh day onwards. Thus, the different rates of hatchability of eggs from different laying days cannot be attributed to the concentrations of total lipids, although some specific lipids such as cholesterol ester and sphingomyelin might influence the difference in larval hatchability, more researches are needed for clarify the role of these substrates in embryogenesis / Objetivando preencher algumas lacunas sobre os mecanismos intr?nsecos da biologia da oviposi??o de R. (B.) microplus, foram avaliadas as concentra??es de lip?dios e carboidratos, assim como a variabilidade lip?dica dos ovos postos em diferentes dias de postura. Para isso, f?meas ingurgitadas pesando entre 151 e 360 mg foram colocadas em estufa biol?gica sob condi??es controladas (27 ? 1oC, 80 ? 5% UR, escotofase), e ap?s in?cio da postura, tr?s amostras di?rias do ?pool? de ovos foram coletadas, acondicionadas e preservadas a ?20?C. As amostras 1 e 2, pesando 50 mg cada, foram utilizadas para dosar lip?dios e glicose, e avaliar o perfil de lip?dios, respectivamente; a amostra 3, contendo 1 g de ovos foi utilizada para dosar glicog?nio. A dosagem de lip?dios totais foi realizada atrav?s do reagente de fosfo-vanilina, enquanto as concentra??es de colesterol, triglicer?dios e glicose foram obtidas atrav?s de determina??o colorim?trica com a utiliza??o de kit enzim?tico de ponto final. A dosagem de glicog?nio foi realizada atrav?s do reativo ?cido 3,5 dinitrosalic?lico. Os resultados das dosagens foram analisados pelo teste n?o param?trico de Kruskal-Wallis e a rela??o entre as concentra??es dos substratos e os dias de postura foi avaliada atrav?s de Regress?o polinomial. N?o foram constatadas diferen?as significativas entre as concentra??es destes substratos nos ovos de diferentes dias de oviposi??o, assim como n?o houve rela??o dessas concentra??es com os dias de postura. Ap?s extra??o dos lip?dios das amostras, foram realizadas cromatografias em camada delgada de alto desempenho (HPTLC) para lip?dios neutros e fosfolip?dios. Em seguida, a densitometria foi realizada atrav?s do programa Image Master Total Lab e o percentual di?rio de cada lip?dio nas amostras foi calculado. Dentre os lip?dios neutros foram identificados hidrocarbonetos, colesterol esterificado, triglicer?dios, ?cidos graxos, colesterol livre e diacilglicerol. Destes, o colesterol esterificado foi o que apresentou maior varia??o, n?o sendo detectado nos ovos do quinto dia de oviposi??o. Dentre os fosfolip?dios foram detectados o ?cido fosfat?dico, fosfatidiletanolamina, fosfatidilcolina, fosfatidilinusitol e esfingomielina. Sendo este ?ltimo ausente nos ovos do s?timo dia de postura em diante. Assim, as diferentes taxas de eclodibilidade por dia de postura n?o podem ser atribu?das ?s concentra??es de lip?dios totais, embora alguns lip?dios espec?ficos como o colesterol esterificado e a esfingomielina possam ter influ?ncia na diferen?a de eclodibilidade larval, sendo necess?rios mais estudos para eclarecer o papel desses substratos na embriog?nese
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Teores de glomalina e subst?ncias h?micas em diferentes est?gios sucessionais de floresta seca / Glomalin and humic substances levels in different sucessional stages of a dry forest

DINIZ, Jurema Diniz 02 June 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-14T14:46:35Z No. of bitstreams: 1 2011 - Jurema Schinz Diniz.pdf: 1784426 bytes, checksum: ef765e40d98144beee9ab74d12aff5f8 (MD5) / Made available in DSpace on 2016-09-14T14:46:35Z (GMT). No. of bitstreams: 1 2011 - Jurema Schinz Diniz.pdf: 1784426 bytes, checksum: ef765e40d98144beee9ab74d12aff5f8 (MD5) Previous issue date: 2011-06-02 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This study contributes to understanding the relationship between tropical dry forests regeneration and arbuscular mycorrhizal fungi activity. The specific objective of this study is to extract and quantify glomalin, glomerospores and humic substances in different sucessional stages of a dry forest. The main objective is to study the activity of AMFs in different stages of a dry forest and to colabore to the projects: ?Functional links between aboveground changes and belowground activity with land use in the Americas: Soil biodiversity and food security? e ?Human, Ecological and Biophysical Dimensions of Tropical Dry Forest?. Total Glomalin (TG) and carbon levels were higher in inicial and late stage of dry forest, indicating the carbon storage contribution to glomalin in soil. In addition, the higher number of glomerospores in this sucessional stage shows the more activity of AMF and its potential to regeneration of disturbed dry forests. The different chemical and physical properties of soil in intermediate stage possibly contributed to low glomalin and carbon levels. The higher levels of humic substances in this stage possibly contribute to the lower activity of AMF and can be explained by the higher diversity of plants in this area. It is important to study the influence of physical and chemical properties, humic substances and plant diversity in AMF activity, in different sucessional stages of dry forests. / Este trabalho contribui para o entendimento da rela??o da regenera??o de florestas tropicais secas, com a atividade dos fungos micorr?zicos arbusculares (FMAs). Tem como objetivos espec?ficos: a extra??o e a quantifica??o de glomalina, de glomerosporos e de subst?ncias h?micas em diferentes sucess?es de floresta seca. O objetivo ? avaliar a atividade dos FMAs em diferentes est?gios sucessionais de floresta seca, acrescentando e colaborando assim para o desenvolvimento dos projetos: ?Functional links between aboveground changes and belowground activity with land use in the Americas: Soil biodiversity and food security? e ?Human, Ecological and Biophysical Dimensions of Tropical Dry Forest?. Os teores de glomalina total e de carbono org?nico foram maiores na ?rea de sucess?o inicial e tardia de floresta seca, indicando maior influ?ncia do estoque de carbono na glomalina do solo. O maior n?mero de esporos e glomalina, na ?rea de sucess?o inicial, mostrou a maior atividade dos fungos micorr?zicos arbusculares em ?rea perturbada que est? se regenerando, sugerindo a contribui??o dos FMAs para essa recupera??o do ecossistema de florestas secas. As propriedades f?sicas e qu?micas diferentes do solo na ?rea de sucess?o intermedi?ria provavelmente contribu?ram para a baixa concentra??o de glomalina e carbono. Os maiores teores de ?cidos h?micos e f?lvicos no solo de sucess?o intermedi?ria podem ter contribu?do tamb?m para a baixa atividade dos FMAs e podem ser devidos ? maior diversidade de esp?cies vegetais nesta ?rea. ? importante desenvolver estudos que comparem as propriedades qu?micas e f?sicas do solo com a atividade desses microorganismos simbiontes em diferentes sucess?es vegetais; que analisem a influ?ncia das subst?ncias h?micas na atividade dos FMAs em campo; e da influ?ncia da diversidade de esp?cies vegetais na atividade dos FMAs.
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Identifica??o de enterobact?rias atrav?s da t?cnica de MALDI-TOF MS e compreens?o da dissemina??o destes agentes em ambiente de produ??o leiteira / enterobacteria identification by MALDI-TOF MS technique and understanding the spread of these agents in dairy production environment

Rodrigues, Naiara de Miranda Bento 26 February 2016 (has links)
Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-09-20T14:14:37Z No. of bitstreams: 1 2016 - NAIARA DE MIRANDA BENTO RODRIGUES.pdf: 1508318 bytes, checksum: bbf2aebb5d43033339bf559d73461919 (MD5) / Made available in DSpace on 2016-09-20T14:14:37Z (GMT). No. of bitstreams: 1 2016 - NAIARA DE MIRANDA BENTO RODRIGUES.pdf: 1508318 bytes, checksum: bbf2aebb5d43033339bf559d73461919 (MD5) Previous issue date: 2016-02-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPQ / Mastitis adversely affects milk production and in general cows do not regain their full production levels post recovery, leading to considerable economic losses. Moreover the percentage decrease in milk production depends on the specific pathogen that caused the infection and enterobacteria are responsible for this greater reduction. These microorganisms are preferentially found in the habitat of animals in places contaminated with feces, urine, clay and also organic beds. Phenotypic tests are among the currently available methods used worldwide to identify enterobacteria; however they tend to misdiagnose the species despite the multiple tests carried out and they can delay the antibiotic therapy by clinic veterinary. On the other hand the MALDI-TOF MS technique has been attracting attention for its precise identification of several microorganisms at species level. In the current study, 183 enterobacteria were detected in milk (n=47) and fecal samples (n=94) collected from cows; also water (n=23) and milk line samples (n=19) collected from a farm in Rio de Janeiro with the purpose to present the MALDI-TOF MS technique as efficient methodology and also as a ?gold standard? to better understand the possible current biochemical errors in enterobacteria identification considering isolates from bovine environments. This proteomic technique confirmed 92.9% (170/183) of the enterobacteria species identified by biochemical tests that showed high sensitivity (> 81%) and specificity (> 89%). The gyrB sequencing was made in eigth from thirteen misidentified enterobacteria and confirmed 100% the MALDI-TOF results, so the proteomic technique was used as a ?gold standard? for this study. The amino acid decarboxylation test made the most misidentifications and Enterobacter spp was the largest misidentified genus (76.9%, 10/13). E.coli was prevalent (83%, 152/183) in all samples and the bovine milk presented the most enterobacteria diversity. The Salmonella sp wasn?t detected in feces bovine samples and all water samples from different points in the farm presented unacceptable microbiological standards. Was identified enterobacteria in milkers hands and nasal cavity also in the milking machines used on the property. These results aim to contribute significantly to the characterization of the Enterobacteriaceae as well in understanding of its spread in dairy production environment , assisting in need diagnostic of possible agents involved in bovine mastitis as well as to implement properly targeted prophylactic measures. / A mastite bovina afeta negativamente a produ??o de leite dificultando a recupera??o dos n?veis de produ??o total das propriedades leiteiras, levando a perdas econ?micas consider?veis. Esta redu??o no percentual da produ??o de leite pode estar associada ao agente patog?nico espec?fico que causou a infec??o, sendo as enterobact?rias frequentemente respons?veis pela mastite ambiental. Estes microrganismos s?o preferencialmente encontrados no habitat normal dos animais como locais que apresentam esterco, urina, barro e camas org?nicas. Os testes fenot?picos est?o entre os m?todos dispon?veis atualmente utilizados para identificar as enterobact?rias; no entanto, eles podem ocasionalmente identitificar erroneamente algumas esp?cies apesar dos m?ltiplos ensaios realizados. Al?m disso, a demora na sua execu??o pode tardar a antibioticoterapia realizada em campo. Por outro lado, a t?cnica de MALDI-TOF MS tem atra?do a aten??o pela sua identifica??o precisa dos v?rios microorganismos em n?vel de esp?cie. No presente estudo, um total de 183 enterobact?rias foram isoladas a partir de amostras de leite (n=47) e fezes colhidas de vacas em lacta??o (n=94); amostras de ?gua (n=23) e na linha de ordenha (n=19) em uma propriedade situada no Rio de Janeiro. A proposta foi utilizar a t?cnica de MALDI-TOF MS como um m?todo eficaz de identifica??o bacteriana de enterobact?rias e descrever a permanencia destes microrganismos no ambiente de produ??o leiteira. A t?cnica prote?mica confirmou 92,9% (170/183) das esp?cies de enterobact?rias identificadas pelos testes bioqu?micos convencionais. O sequenciamento do gene gyrB, realizado em oito das 13 enterobact?rias que apresentaram identifica??o discordante, confirmou em 100% o resultado da t?cnica prote?mica, que foi utilizada como metodologia de refer?ncia no presente estudo. O g?nero Enterobacter foi o mais discordante pelo m?todo bioqu?mico (76,9%, 9/13). A E.coli foi a esp?cie predominante (83%, 152/183) em todas as amostras avaliadas, sendo que o leite bovino apresentou maior diversidade de enterobact?rias. N?o foi detectada a presen?a de Salmonella spp. nas amostras de fezes bovinas e todas as amostras de ?gua dos diferentes pontos de coleta da propriedade apresentaram padr?es microbiol?gicos inaceit?veis. Foram isoladas enterobact?rias das m?os e cavidades nasal dos ordenhadores, bem como nas ordenhadeiras mec?nicas utilizadas na propriedade. Estes dados visam contribuir de forma significativa para a caracteriza??o das enterobacterias bem como para a compreens?o e sua descri??o no ambiente de produ??o leiteira, auxiliando no diagn?stico preciso dos poss?veis agentes envolvidos na mastite bovina bem como na implementa??o de medidas profil?ticas devidamente direcionadas.

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