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Previous issue date: 2006-11-24 / The intake of mycotoxin-contamined feeds may lead to nutrient losses and can have
adverse effects on animal health and on productivity. The aims of this study were: 1) to
determine the mycobiota present in poultry feed samples, obtained from three factories
in the region of S?o Jos? do Vale do Rio Preto, RJ, Brazil; 2) to evaluate the natural
occurrence of aflatoxin B1 (AFB1), fumonisin B1 (FB1) and zearalenone using the
method enzyme linked immunosorbent assay (ELISA) competitive, commercial kits of
Beacon Analytical Systems Inc, USA and 3) to verify the data results by the ELISA to
AFB1 and FB1 comparing them with the high precision liquid chromatography
methodology (HPLC) Fungal counts were similar between all culture media tested (103
CFU.g-1). Penicillium spp. (41,26%) was the most prevalent genus followed by
Aspergillus spp. (33,33%) and Fusarium spp. (20,63%). Aflatoxin B1 was found in
66,27% samples examined by ELISA kits and values ranged between 2 and 21μg.kg-1.
Fumonisin B1 was found in 97,87% and levels ranged between 0,3 and 9,1μg.g-1, cooccurring
with aflatoxin B1 in 66,7% of the samples. Low concentrations of zearalenone
were found (<1 μg.g-1) in 77,1% poultry feed samples. Correlation between the ELISA
kits (AFB1 and FB1) and the HPLC analysis was obtained. Values were r2= 0,9922 and
r2=0,9823, respectively. In general, the highest mycotoxin levels were found during the
hottest month of sampling. The present study shows the simultaneous occurrence of two
carcinogenic mycotoxins, aflatoxin B1 and fumonisin B1 together with another
Fusarium mycotoxin (zearalenone) in feed intended for poultry consumption. Many
samples contained AFB1 levels near the maximum permissible and it could affect young
animals. A synergistic toxic response is possible in animals under simultaneous
exposure. / A ingest?o de ra??es contaminadas por micotoxinas pode acarretar perdas no seu valor
nutritivo e causar efeitos adversos ? sa?de animal e ? produtividade. Os objetivos desse
estudo foram: 1)estabelecer a freq??ncia e a preval?ncia da micobiota presente em
amostras de ra??es de frangos de corte provenientes de tr?s f?bricas em S?o Jos? do
Vale do Rio Preto-RJ, 2)verificar a ocorr?ncia natural de aflatoxina B1 (AFB1),
fumonisina B1 (FB1) e zearalenona (ZEA), utilizando a t?cnica biol?gica de
imunoensaios-ELISA competitivo, com kits comerciais (Beacon Analytical Systems
Inc, USA) e 3)verificar os resultados obtidos pelo m?todo de ELISA, comparando-os
com a t?cnica de Cromatografia L?quida de Alta Efici?ncia (CLAE) para AFB1 e FB1. A
contagem dos prop?gulos f?ngicos foi similar entre todos os meios de cultura testados
(103 UFC g-1). Penicillium spp (41,26%) foi o g?nero de maior preval?ncia seguido por
Aspergillus spp (33,33%) e Fusarium spp. (20,63%). Aflatoxina B1 foi encontrada em
66,27% das amostras analisadas pelos kits de ELISA,com valores variando entre 2 e
21μg.kg-1. Fumonisina B1 foi encontrada em 97,87% e os valores variaram entre 0,3 e
9,1μg.g-1, co-ocorrendo com aflatoxinaB1 em 66,7% das amostras. Baixas
concentra??es de zearalenona foram encontradas (< 1μg.g-1)em 77,1% das amostras de
ra??o. Foi obtida correla??o entre os kits de ELISA (AFB1 e FB1) e as an?lises em
CLAE, com valores de r2= 0,9922 e 0,9823, respectivamente. Em geral, os maiores
n?veis de micotoxinas foram encontrados durante os meses mais quentes do ano. O
presente estudo mostra a ocorr?ncia simult?nea de duas micotoxinas carcinog?nicas,
AFB1 e FB1, juntamente com outra Fusarium micotoxina (zearalenona) em ra??es
destinadas ao consumo de frangos. Algumas amostras apresentaram n?veis de AFB1
pr?ximos ao m?ximo permitido, o que poderia afetar aves jovens. Uma resposta t?xica
sin?rgica ? poss?vel em animais sob exposi??o simult?nea.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/863 |
| Date | 24 November 2006 |
| Creators | Oliveira, Glenda Ribeiro de |
| Contributors | Rosa, Carlos Alberto Rocha |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Microbiologia Veterin?ria, UFRRJ, Brasil, Microbiologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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