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Phase Imaging Digital Holography for Biological Microscopy

We apply the techniques of digital holography to obtain microscopic 3D images of biological cells. Both the amplitude and phase images are obtained from a single hologram, with approximately 1 [mu]m lateral and 10 nm longitudinal resolution. The results are combined with previous experiments resolving the 2 [pi] ambiguity to produce continuous phase images of the samples.

Identiferoai:union.ndltd.org:USF/oai:scholarcommons.usf.edu:etd-2190
Date25 March 2004
CreatorsParshall, Daniel
PublisherScholar Commons
Source SetsUniversity of South Flordia
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceGraduate Theses and Dissertations
Rightsdefault

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