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Previous issue date: 2016-02-25 / CNPq / The interaction between intermediate hosts and helminths can cause metabolic and reproductive changes in the host snail, they start using their reserves to maintain its vital functions, immune system activation, repair of tissue damage and also to supply the necessary energy for the parasites development. Investigations of co-infection by different helminths can provide essential information about the biology of their co-existence. Our aims are investigate the reproductive changes, evaluate the activity of lactate dehydrogenase and the concentrations of glucose in the hemolymph and glycogen in the digestive gland complex and cephalopedal mass, and also verify histological and histochemical changes of Biomphalaria glabrata experimentally co-infected with Echinostoma paraensei and Angiostrongylus cantonensis. Five groups of snails i.e. uninfected, with single and double infections, either E. paraensei first (E+A) or A. cantonensis first (A+E) were followed; three times a week during four weeks the numbers of egg masses, eggs and hatched snails were counted. Histological and histochemical samples of the ovotestis, albumen gland, digestive gland complex and cephalopedal mass were collected after four weeks as well samples for the biochemical analysis. The number of egg masses/snail, eggs/snail and hatched snails showed significant differences comparing the control group to all infected groups, especially in group E + A, with the majority of values of parameters analyzed lower than 50% of those observed for control snails. A significant decrease in glucose levels and glycogen content, in contrast an increase of lactate dehydrogenase activity was observed in the infected snails. These results indicate intense energy demand that takes the snail increasing the anaerobic degradation of carbohydrates in the infected snails to obtain energy in an attempt to maintain homeostasis. The histological analysis showed that presence of both parasites in the all organs analyzed and the parasites were associated with cell disorganization. / A intera??o entre hospedeiros intermedi?rios e helmintos pode causar altera??es metab?licas e reprodutivas no molusco hospedeiro, que come?am a usar suas reservas para manter as fun??es vitais, na elabora??o de resposta imunol?gica e repara??o de danos teciduais e tamb?m para compensar a drenagem de nutrientes/energia pelos parasitos em desenvolvimento. Investiga??es sobre as co-infec??es por helmintos de esp?cies diferentes podem fornecer informa??es essenciais sobre a biologia da sua co-exist?ncia, refletindo aspectos mais pr?ximos ?queles que encontramos em condi??es naturais. Nossos objetivos foram investigar as altera??es reprodutivas, avaliar a atividade da lactato desidrogenase e as concentra??es de glicose na hemolinfa e glicog?nio no complexo gl?ndula digestiva-g?nada e massa cefalopediosa, e tamb?m verificar altera??es histol?gicas e histoqu?micas de Biomphalaria glabrata experimentalmente co-infectados com Echinostoma paraensei e Angiostrongylus cantonensis. Cinco grupos de moluscos (n?o infectados, com infec??es simples (Echinostoma paraensei ou Angiostrongylus cantonensis) e dupla, seja E. paraensei primeiro (E + A) ou A. cantonensis primeiro (A + E)) foram acompanhados; tr?s vezes por semana durante quatro semanas o n?mero de massas de ovos, n?mero de ovos e n?mero de moluscos eclodidos foram contados. Amostras para a an?lise histol?gica e histoqu?mica do ovoteste, gl?ndula do alb?men, complexo gl?ndula digestiva e massa cefalopediosa foram coletadas ap?s quatro semanas, assim como amostras para a an?lise bioqu?mica. O n?mero de massas de ovos/molusco, ovos/molusco e moluscos eclodidos dos moluscos infectados apresentaram diferen?as significativas quando comparados com o grupo controle, especialmente no grupo E + A, cuja a maioria dos valores dos par?metros analisados foi inferior a 50% dos valores observados para os moluscos controle. Um decr?scimo significativo nos n?veis de glicose e de glicog?nio, em contraste com um aumento de atividade da lactato desidrogenase foi observado nos exemplares infectados. Estes resultados indicam que a demanda de energia intensa leva o molusco infectado ao aumento da degrada??o anaer?bia de carboidratos para obten??o de energia buscando atender ao aumento da demanda energ?tica, numa tentativa de manter a homeostase glic?mica, por?m redu??es significativas s?o observadas. As an?lises histol?gicas e histoqu?micas mostraram a presen?a de ambos os parasitos nos ?rg?os analisados associados com intensa desorganiza??o celular.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1571 |
| Date | 25 February 2016 |
| Creators | BOMFIM, Tatiane Cristina dos Santos |
| Contributors | Silva, Jairo Pinheiro da, Garcia, Juberlan Silva, Silva, Jairo Pinheiro da, Brandolini, Solange Viana Paschoal Blanco, Mota, Ester Maria |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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