The detection and annotation of cis-regulatory sequences is a difficult problem. There is currently no generally applicable experimental procedure or computational algorithm to identify the non-coding regions of the genome that serve to activate gene expression in a given cell type. The only indicator of cis-regulatory function is the conservation of a sequence in other genomes. Regions can then be tested one-by-one in transgenic assays but this is time-consuming in vertebrates. Only a limited number of these already validated cis-regulatory sequences have been curated in biological databases. One of the main advantages of the model organism Ciona intestinalis is that cis-regulatory tests can be conducted very easily and the result is observable after one day while the animal follows the chordate body plan. However, a sequence found to be active in this organism can currently not be mapped to genomes of other animals.
Identifer | oai:union.ndltd.org:CCSD/oai:tel.archives-ouvertes.fr:tel-00413501 |
Date | 15 July 2009 |
Creators | Häussler, Maximilian |
Publisher | Université Paris Sud - Paris XI |
Source Sets | CCSD theses-EN-ligne, France |
Language | English |
Detected Language | English |
Type | PhD thesis |
Page generated in 0.001 seconds