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Previous issue date: 2005-01-20 / Gerberas are cut flowers with large color diversity and good durability. The use of
growth regulators on in vitro propagation is related to the capacity to induce
proliferation of sprouts and differentiation of roots. However, citokinins in excess can
induce the formation of abnormal plant, with reduced acclimatization potential. The
mineral and organic composition of media culture plays an important role during the
process. The objective of this dissertation was to adjust protocols for in vitro
propagation of Gerbera jamesonii. The following aspects were evaluated: (i) the effect
of the inflorescence developmental stage on the performance of in vitro culture initiated
from chapters (ii) gerbera varieties (iii) growth regulator balance and (iv) the effect of
salt concentration of MS medium on the proliferation of in vitro sprouting and rooting
and acclimatization of three varieties of gerbera. For variety Phanter, the regeneration of
explants prepared from inflorescences depended on its stage of development. Explants
prepared from young chapters showed better results. The concentration and source of
citokinin, and the concentration of salts in MS medium influenced the proliferation of
sprouts in explants of variety Ornela. BAP was more efficient than kinetin on
proliferation of sprouts. Concentrations above 2.0 mg.L-1 of BAP induced the formation
of plants with undesirable characteristics during in vitro multiplication, while this was
not observed in the presence of the same kinetin in larger concentration (4.0 mg.L-1).
The combinations of 0.5 mg.L-1 BAP with the different levels of IBA showed the best
results for multiplication and production of sprouts, with potential for acclimatizing
variety Ornela. The presence of BAP inhibited the differentiation of roots, while the
same was not observed in largest concentration of kinetin. The concentration of salts
below 50% limited the proliferation of sprouts in variety Ornela. The varieties
Capuccino, Mirage and Ornela, when maintained in the same balance of BAP and IBA,
showed divergences in results for number of sprouts; when acclimatized, they presented
high survival rates. Difficulties on in vitro gerbera propagation were mostly found on
the regeneration phase and on the formation of sprouts in explants prepared from
chapters. For the other phases, no major difficulties were detected. / As g?rberas s?o flores de corte que apresentam diversidade na colora??o e boa
durabilidade. O uso de fitorreguladores na propaga??o de plantas in vitro esta
relacionado com a capacidade de induzir a prolifera??o de brotos e a diferencia??o de
ra?zes. No entanto, citocininas em excesso podem induzir a forma??o de pl?ntulas
anormais e com baixo potencial para serem aclimatadas. Al?m disso, a composi??o
mineral e org?nica do meio de cultura apresenta importante papel durante o processo. O
objetivo deste trabalho foi ajustar protocolos para a propaga??o massal in vitro de
variedades de Gerbera jamesonii. Avaliou-se: o efeito do est?dio de desenvolvimento
de infloresc?ncias na implanta??o de culturas in vitro a partir de cap?tulos; variedades;
balan?os de fitorreguladores; concentra??o dos sais do meio MS na prolifera??o de
brotos e enraizamento in vitro e a aclimatiza??o de tr?s variedades de g?rbera. A
regenera??o de explantes preparados a partir de infloresc?ncias depende do est?dio de
desenvolvimento das mesmas. Explantes preparados a partir de cap?tulos jovens
apresentaram melhores resultados. A prolifera??o de brotos em explantes de g?rbera,
variedade Ornela foi influenciada tanto pela concentra??o quanto pela fonte de
citocinina, assim como pela concentra??o dos sais do meio MS. A mol?cula BAP
mostrou ser mais eficiente que kinetina para induzir a prolifera??o de brotos. BAP
acima de 2,0 mg.L-1 induziu a forma??o de plantas com caracter?sticas indesej?veis
durante a multiplica??o in vitro, enquanto isto n?o foi observado mesmo na maior
concentra??o de kinetina (4,0 mg.L-1). As combina??es de 0,5 mg.L-1 de BAP com os
diferentes n?veis de AIB (0; 0,05 e 0,5 mg.L-1) apresentaram os melhores resultados
para a multiplica??o e produ??o de brotos in vitro com potencial para serem aclimatados
da variedade Ornela. Somente o BAP inibiu a diferencia??o de ra?zes. A concentra??o
dos sais abaixo de 50% limitou a prolifera??o de brotos na variedade Ornela. As
variedades Capuccino Mirage e Ornela, quando mantidas no mesmo balan?o de BAP e
AIB apresentaram resultados divergentes quanto ? prolifera??o de brotos, e na
aclimatiza??o apresentaram alta taxa de sobreviv?ncia. Na propaga??o in vitro de
g?rbera, somente na fase de implanta??o das culturas in vitro que verificou-se
dificuldade de regenera??o e na forma??o de brotos em explantes preparados a partir de
cap?tulos. Nas demais fases, as variedades estudadas apresentaram facilidade e
resultados bastante promissores.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/522 |
| Date | 20 January 2005 |
| Creators | Sousa, Cleiton Mateus |
| Contributors | Miranda, Ricardo Motta |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Fitotecnia, UFRRJ, Brasil, Ci?ncias Agr?rias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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