CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / O ambiente estuarino à reconhecidamente um local de aporte de Ãguas contaminadas de diferentes fontes. O gÃnero Vibrio à encontrado, naturalmente, nesses locais, sendo relatadas, em inÃmeros trabalhos cientÃficos, cepas resistentes a antimicrobianos utilizados, rotineiramente, no tratamento mÃdico. Foram selecionadas setenta cepas de Vibrio oriundas de amostras de Ãgua e sedimento do estuÃrio do Rio AcaraÃ, litoral Oeste do CearÃ, Brasil para o experimento. O antibiograma foi proposto em duas baterias, sendo uma com Ãgar Mueller-Hinton solubilizado em Ãgua destilada (MH+AD) e a outra em Ãgua do mar (MH+AM), alÃm de ser testada a presenÃa dos fatores de virulÃncia proteases (caseinase, elastase e gelatinase), lipases, fosfolipases, DNases, amilases, urease e hemolisinas (β-hemÃlise pelo teste de Kanagawa em Ãgar Wagatsuma). Os antimicrobianos testados foram NAL, AMP, ATM, CFL, CIP, CLO, CTX, EST, GEN, OTC, PEN, SUT e TET. Em MH+AD, o perfil de resistÃncia foi verificado para 47 (67,1%) cepas a PEN, 26 (37,1%) cepas a AMP e CFL, 8 (11,4%) a OTC, 6 (8,6%) a TET, 3 (4,3%) a ATM, e 1 (1,4%) a CLO, enquanto para MH+AM, 68 (97,1%) cepas foram resistentes a OTC, 67 (95,7%) a TET, 57 (81,4%) a PEN, 34 (48,6%) a CFL, 29 (41,4%) a AMP, 21 (30,0%) a EST, 20 (28,5%) a GEN, 5 (7,2%) a ATM e NAL, 3 (4,3%) a SUT e 1 (1,4%) a CIP. As cepas resistentes em MH+AD foram submetidas à cura do plasmÃdio pelo agente curagÃnico acridine Orange. Foi verificada resistÃncia plasmidial em 36,21% das cepas resistentes a AMP, 5,17% a ATM, 37,93% a CFL, 13,79% a OTC, 53,45% a PEN e 6,90% a TET. Por ordem de recorrÃncia, 67 (91,42%) cepas de Vibrio spp. analisadas apresentam atividade da enzima urease, seguidas de 57 (81,42%) para lipase, 54 (77,14%) a amilase, 53 (75,72%) a gelatinase, 43 (61,42%) a caseinase, 26 (37,14%) a fosfolipase, 17 (24,28%) a DNase, 16 (22,86%) a elastase e 13 (18,57%) a β-hemÃlise (Kanagawa). A Ãgua do mar influenciou, significativamente, a caracterizaÃÃo da resistÃncia das cepas de Vibrio spp. analisadas, principalmente, sobre os antimicrobianos das classes das tetraciclinas, alÃm de ter sido detectada mÃltipla resistÃncia associada à cepas com fatores preliminares de virulÃncia. / The estuarine environment is recognized as an intake of contaminated water from different sources. The genus Vibrio is found in those places, being reported in many papers resistant strains to antibiotics used routinely in medical treatment. Seventy strains of Vibrio were selected originated from samples of water and sediment from the Acaraà estuary, West coast of CearÃ, Brazil to experiment. The antibiogram was proposed in two batteries, one with Mueller Hinton agar dissolved in distilled water (MH+DW) and the other in seawater (MH+SW), and is tested the presence of virulence factors protease (caseinase, elastase and gelatinase), lipases, phospholipases, DNases, amylase, urease, and hemolysin (β-hemolysis test by Kanagawa Wagatsuma agar). The antimicrobials tested were NAL, AMP, ATM, CFL, CIP, CHL, CTX, STP, GEN, OTC, PEN, TCY and STX. In MH+DW, the resistance profile was observed for 47 (67.1%) strains to PEN, 26 (37.1%) to AMP and CFL, 8 (11.4%) to OTC, 6 (8.6%) to TCY, 3 (4.3%) to ATM and 1 (1.4%) to CHL, while for MH+SW, 68 (97.1%) strains were resistant to OTC, 67 (95.7%) to TCY, 57 (81.4%) to PEN, 34 (48.6%) to CFL, 29 (41.4%) to AMP , 21 (30.0%) to STP, 20 (28.5%) to GEN, 5 (7.2%) to ATM and NAL, 3 (4.3%) to STX and 1 (1.4%) to CIP. The resistant strains in MH+DW were subjected to plasmid curing by acridine orange agent. Resistance plasmid was observed in 36.21% of resistant strains to AMP, 5.17% to ATM, 37.93% to CFL, 13.79% to OTC, 53.45% to PEN and 6.90% to TCY. By order of recurrence, 67 (91.42%) strains of Vibrio spp. have analyzed the enzyme urease, followed by 57 (81.42%) to lipase, 54 (77.14%) to amylase, 53 (75.72%) to gelatinase, 43 (61.42%) to caseinase, 26 (37.14%) to phospholipase, 17 (24.28%) to DNase, 16 (22.86%) to elastase and 13 (18.57%) to β-hemolysis (Kanagawa). Seawater influence significantly the characterization of the resistance of strains of Vibrio spp. analyzed mainly on the drugs of the tetracycline class, and has been detected multidrug resistant strains associated with preliminary virulence factors.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:4608 |
| Date | 25 March 2011 |
| Creators | Rafael dos Santos Rocha |
| Contributors | Regine Helena Silva dos Fernandes Vieira, Silvana Saker Sampaio, Oscarina Viana de Sousa |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Engenharia de Pesca, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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