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Previous issue date: 2009-02-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study was realized with the objective of to evaluate the inhibitory activity of the insect
growth regulator, fluazuron, on evolutive stages and adults of Ctenocephalides felis felis.
Twelve beagle dogs were utilized divided into two groups of six animals on day 0 (treatment
day). Group 1: Dogs were treated with a pour on formulation of 2.5% fluazuron, in the dosis
of 10 mg/kg body weight and; Group 2: Dogs were maintained untreated (control). Each dog
was infested with 200 specimens of C. felis felis. Reinfestations were performed on days +5,
+12, +19, +26, +33, and +40. Seventy four hours after each infestation, animals were placed
in individual pet transport boxes for four hours. Afterwards, the material present in the bottom
of each box was collected and the found eggs were quantified. Half of the eggs were
incubated with 0.5g of an artificial diet for larval maintenance in a BOD incubator in the
temperature of 28?C and relative humidity of 75 ? 10%. Seven days after, the material were
fixed in 70% ethanol and evaluated for larval eclosion. After 25 days, half rest were also fixed
in 70% ethanol and evaluated for the emergency of adult fleas. Each dog was combed until
complete removal of fleas. Thus, fleas were quantified sexed, being evaluated the interference
caused by fluazuron on adult recovery, and possibly, a different effect per gender. In order to
evaluate the activity of fluazuron on oviposition of C. felis felis, the ratio between egg
production and number of recovered females. The efficacy of fluazuron on C. felis felis
development from egg to larvae on days +8, +15, +22 and +29 was higher 80%. For the
development from egg to adult, the efficacy remained above 80% for 22 days after treatment,
reaching 100% on day +15. A significant aulticidal efficacy of fluazuron was observed on
days +8; +22; +36 e +43. Regarding to female fleas was observed significant difference on
days +8; +22 e +36 and, for males on days +8 and +36. The efficacy of fluazuron on the
inhibition of oviposition did not presented significance throughout the 43 experimental days.
Fluazuron has efficacy on the development from egg to larva and egg to adult of C. felis felis.
However, it shows low adulticidal activity, and does not interfere on fleas?s oviposition. / Este trabalho foi realizado com o objetivo de avaliar a atividade inibit?ria do regulador de
crescimento de insetos, fluazuron, sobre as formas evolutivas e adultos de Ctenocephalides
felis felis. Foram utilizados 12 c?es da ra?a Beagle, e, no dia 0, dia do tratamento, foram
divididos em dois grupos: Grupo 1. Seis c?es tratados com formula??o pour on , de
fluazuron a 2,5 %, empregando-se a dose de 10 mg por Kg de peso corporal e Grupo 2. Seis
c?es mantidos como controle, sem tratamento. Cada c?o foi infestado com 200 esp?cimes de
C. felis felis. Novas infesta??es foram realizadas nos dias +5, +12, +19, +26, +33, e +40.
Setenta e duas horas ap?s cada infesta??o, os c?es foram alocados em transportes para c?es,
por quatro horas. Ap?s esse per?odo, o material foi coletado e os ovos quantificados. Metade
dos ovos foi incubada com 0,5 grama de uma dieta para manuten??o das larvas, em c?mara
climatizada mantida na temperatura de 28?C e umidade relativa de 75 ? 10%. Ap?s sete dias,
o material foi fixado em ?lcool 70% e avaliado para eclos?o de larvas. Vinte cinco dias
depois, a metade restante foi fixada tamb?m com ?lcool 70% e avaliada para emerg?ncia de
pulgas adultas. Cada c?o foi penteado para retirada total das pulgas, que foram quantificadas e
sexadas, sendo avaliada a interfer?ncia do fluazuron sobre a recupera??o de adultos e poss?vel
atua??o diferenciada por sexo. Para a avalia??o da atividade do fluazuron sobre a oviposi??o
de C. felis felis, foi calculada a rela??o entre a produ??o de ovos e o n?mero de f?meas
recuperadas. A efic?cia do fluazuron sobre o desenvolvimento de ovo a larva de C. felis felis,
nos dia +8; +15; +22 e +29, foi superior a 80%. No desenvolvimento de ovo a adulto de C.
felis felis, a efic?cia permaneceu acima de 80% nos primeiros 22 dias ap?s o tratamento, e, no
dia +15, o fluazuron alcan?ou 100% de efic?cia. No presente estudo, nos dias +8; +22; +36 e
+43, foi constatada uma efic?cia adulticida significativa no grupo tratado com fluazuron. Em
f?meas, houve diferen?a significativa nos dias +8; +22 e +36, e em machos nos dias +8 e +36.
Ao longo dos 43 dias experimentais, a efic?cia do fluazuron na inibi??o da oviposi??o n?o
apresentou signific?ncia. O fluazuron possui efic?cia sobre o desenvolvimento de ovo a larva
e de ovo a adulto de C. felis felis. Entretanto, apresenta baixa atividade adulticida, e n?o
interfere na oviposi??o das pulgas.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/777 |
| Date | 26 February 2009 |
| Creators | Vieira, Vanessa Paulino da Cruz |
| Contributors | Borja, Gonzalo Efrain Moya, Scott, Fabio Barbour |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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