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Previous issue date: 2008-04-15 / This work was developed in the Department of Microbiology and Immunology of the Institute
of Veterinary Rural Federal University of Rio de Janeiro in Serop?dica-RJ.
We evaluated the antifungal activity plant Cymbopogon citratus (lemon-grass), Peumus
boldus (boldo) and Shinus terebinthifolia (aroeira), inhibition of growth of species of the
genus Aspergillus (A. flavus, A. niger, A. ochraceus, A. Parasiticus and A. carbonarius. Using
the method of minimum inhibitory concentration in agar, with the technique of dilution plate
(Pour-Plate), were held dilutions of different extracts, resulting in the final concentrations of
5%, 2.5% and 1.25%. Testing of commercial sensitivity to antifungal - ketoconazole were
made in a final concentration of 1933.18 ? g /mL, as recommended by the National
Committee for Clinical Laboratory Standards (NCCLS). The results obtained in the
experiments have shown that aqueous extracts of C. citratus and S. terebinthifolia, after 24
hours of incubation, were able to inhibit the growth of A. flavus and A. carbonarius,
respectively. The other extracts being studied and tested in the concentrations were not able to
inhibit the growth of mycelial species of the genus Aspergillus. / Este trabalho foi desenvolvido no Departamento de Microbiologia e Imunologia do Instituto
de Veterin?ria da Universidade Federal Rural do Rio de Janeiro, em Serop?dica-RJ. Foram
avaliadas a atividade antif?ngica das plantas Cymbopogon citratus (capim-lim?o), Peumus
boldus (boldo) e Shinus terebinthifolia (aroeira), na inibi??o do crescimento de esp?cies do
g?nero Aspergillus (A. flavus, A. niger, A. ochraceus, A. parasiticus e A. carbonarius).
Utilizando o m?todo da concentra??o inibit?ria m?nima em ?gar, com a t?cnica de dilui??o em
placa (Pour-Plate), foram realizadas dilui??es dos diferentes extratos, obtendo-se as
concentra??es finais de 5%, 2,5% e 1,25%. Testes de sensibilidade ao antif?ngico comercial -
cetoconazol foram realizados numa concentra??o final de 1933,18 ?g/mL, conforme
recomendada pelo National Committee for Clinical Laboratory Standards (NCCLS). Os
resultados obtidos nos experimentos demonstraram que os extratos aquosos de C. citratus e S.
terebinthifolia, ap?s 24 horas de incuba??o, foram capazes de inibir o crescimento de A.
flavus e A. carbonarius, respectivamente. Os demais extratos em estudo e nas concentra??es
testadas, n?o foram capazes de inibir o crescimento micelial das esp?cies do g?nero
Aspergillus.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/873 |
| Date | 15 April 2008 |
| Creators | Santos, Alexander |
| Contributors | Borba, Helcio Resende |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Microbiologia Veterin?ria, UFRRJ, Brasil, Microbiologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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