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Previous issue date: 2008-02-21 / This work characterized the specific cellular immune response of TCD4 and
TCD8 lymphocytes against recombinant Mycobacterium tuberculosis at the
Hospital Anuar Auad, Goiania Brazil, and constituted of two experimental
groups: 1) 22 active TB patients with positive acid fast aputum, X-ray indicative
of tuberculosis, smear culture positive for M. tuberculosis and HIV negative. 2)
15 sex and age matched healthy controls, tuberculin skin test and HIV negative.
Venous blood was drawn and processed to obtain PBMC that were cultivated
for 96 hours with the specific antigens (1mg/106 cells). TCD8 and TCD4 cells
were analyzed by flow citometry for IL-10 and IFN-g production. In general, the
percentage of positive TCD4 and TCD8 cells for IFN-g and IL-10 were superior
among the TB patients. Additionally, TCD4+IFNg+ (5,63±2,43) and IL-10+ (5,83±
2,19) cells were significantly higher in TB patients than in healthy controls
(TCD4+IFNg+ =1,75±0,71 and IL-10+ =1,47±0,90), (p<0,01). Regarding the
percentage of TCD8 cells, a higher percentage of IFNg+ (4,33±1,45) and IL-10+
(4,01±1,14) among TB patients than controls (TCD8+IFNg+ = 1,49±0,42 and IL-
10+ 1,62±0,59) was observed (p<0,01). TB treatment did not alter the response to the tested antigens immediately after the treatment initiation. In conclusion,
the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by
the specific immune response of active TB patients.
Key words: Tuberculosis, Mycobacterium tuberculosis, Recombinant antigens,
Cellular immune response.
to the tested antigens immediately after the treatment initiation. In conclusion,
the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by
the specific immune response of active TB patients. / Este trabalho avaliou a resposta imune celular dos linfócitos TCD4 e TCD8 de
pacientes com tuberculose pulmonar ativa antes e após o tratamento, contra os
antígenos recombinantes MPT-51, ESAT-6, GLcB, Ag85A e o Filtrado Protéico
de Cultura (CFP) de Mycobacterium tuberculosis atendidos no Hospital de
Doenças Tropicais Anuar Auad. A população de estudo, composta de 37
indivíduos, foi dividida em dois grupos experimentais. 1) 22 pacientes com
tuberculose pulmonar, selecionados de acordo com idade, diagnóstico
confirmado por baciloscopia (BAAR), radiografia evidente de tuberculose,
HIV1/2 negativos. 2) 15 controles saudáveis negativos para prova tuberculinica
(PT) e HIV1/2, pareados por faixa etária e sexo aos pacientes selecionados.
Coletou-se aproximadamente 20 ml de sangue com heparina. O sangue total
foi processado e cultivado por 96 horas na presença dos antígenos
recombinantes e os linfócitos TCD4 e TCD8 foram analisados quanto à
positividade para as citocinas IL-10 e IFN-g por citometria de fluxo. As
porcentagens de linfócitos responsivos aos diversos antígenos de M.
tuberculosis apresentaram-se em geral superiores aos controles. A
porcentagem dos linfócitos TCD4+IFNg+ (5,63±2,43) e IL-10+ (5,83±2,19) foram
maiores em pacientes com tuberculose pulmonar ativa em comparação com os
linfócitos TCD4+IFNg+ (1,75±0,71) e IL-10+ (1,47±0,90) dos controles (p<0,01).
Também, as porcentagens dos linfócitos TCD8+IFNg+ (4,33±1,45) e IL-10+ (4,01
±1,14) dos pacientes foram maiores que os linfócitos TCD8+IFNg+ (1,49±0,42) e
IL-10+ (1,62±0,59) dos controles (p<0,01). Não houve diferença significativa
antes (5,63±2,43) e após (5,0±2,0) o tratamento nas populações celulares
TCD4+ e TCD8+ positivas para IFN-g e IL-10 quando estimuladas com o painel de antígenos (p>0,05). Com estes resultados podemos considerar que todos os
antígenos testados (MPT-51, ESAT-6, GLcB, Ag85A) são reconhecidos pelos
linfócitos TCD4+ e TCD8+ de pacientes com tuberculose pulmonar ativa.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tde/1818 |
| Date | 21 February 2008 |
| Creators | VASCONCELOS JUNIOR, Arioldo Carvalho |
| Contributors | KIPNIS, Ana Paula Junqueira |
| Publisher | Universidade Federal de Goiás, Mestrado em Medicina Tropical, UFG, BR, Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
| Rights | info:eu-repo/semantics/openAccess |
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