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Previous issue date: 2015-07-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / V?rios estudos tem estabelecido uma associa??o entre Diabetes Mellitus tipo 1 altera??es hep?ticas estresse oxidativo. No entanto os conhecimentos de como essas altera??es ocorrem no sistema antioxidante ainda n?o est? elucidado. Este estudo avaliou os efeitos de quatro semanas de diabetes tipo 1 no tecido hep?tico. Ratos machos wistar foram divididos em 2 grupos: Controle (C) e Diab?tico (D). A an?lise da atividade de enzimas antioxidantes (CAT e SOD); a express?o do mRNA de CAT, SOD1, SOD2, GPX1 e PRX4 foram avaliadas; marcadores de estresse oxidativo tamb?m foram avaliados (Peroxida??o de lip?deos, carbonila??o de prote?nas e conte?do tiol) e conte?do de H2O2 hep?tico. Como resultado a diabetes aumentou a atividade da SOD e a express?o g?nica da SOD2, enquanto diminuiu a express?o da SOD1. Assim como houve diminui??o da atividade da CAT e na express?o g?nica da CAT, GPX1 e PRX4. Houve tamb?m mudan?as em biomarcadores de estresse oxidativo. Nossos resultados sugerem que provavelmente as quatro semanas da diabetes, induzem altera??es precoces no sistema antioxidante no f?gado de ratos induzidos por STZ, agravando altera??es decorrentes do estresse oxidativo levando a forma??o de esp?cies reativas de oxig?nio, podendo contribuir para um preju?zo da fun??o hep?ticas. / The correlation between the type 1 diabetes mellitus and oxidative stress have been described in several studies, however its underlying mechanisms are not fully elucidated. The present work aimed to evaluate the effects of four weeks of streptozootocin-induced (STZ) diabetes in the redox homeostasis of rat hepatocytes. Thus, the liver of male Wistar rats from control and diabetic groups were collected and the activity and expression of antioxidant enzymes, as well the main markers of oxidative stress and content of H2O2 in these tissues were measured. The diabetes induced the activity of superoxide dismutase (SOD) and the gene expression of its mitochondrial isoform, SOD2. However, the expression of SOD1, the cytoplasmic isoform, was reduced by this disease. The activity and expression of catalase (CAT), as well the expression of glutathione peroxidase 1 (GPX1) and peroxiredoxin 4 (PRX4) were drastically reduced in the hepatocytes of diabetics rats. Even with this debility in the peroxidases mRNA expression, the content of H2O2 was reduced in the liver of diabetics rats when compared to the control group. The diabetes caused an increase of lipid peroxidation and a decrease of protein thiol content, showing that this disease causes distinct oxidative effects in different cell biomolecules. Our results indicate that four week of diabetes induced by STZ is already enough to compromise the enzymatic antioxidant systems of the hepatocytes.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20734 |
| Date | 29 July 2015 |
| Creators | Almeida, R?mulo Rodrigo de Souza |
| Contributors | 79332021368, http://lattes.cnpq.br/3289758851760692, Rocha, C?ntia Renata Costa, 10328941808, http://lattes.cnpq.br/8356963705328584, Pasquali, Matheus Augusto de Bittencourt, 97496065072, http://lattes.cnpq.br/2819075769518114, Abreu, Bento Jo?o da Gra?a Azevedo, Lima, Jo?o Paulo Matos Santos |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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