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Previous issue date: 2017-05-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O c?ncer do colo do ?tero ? o terceiro tipo de c?ncer mais frequente em mulheres no
Brasil, sendo a sua frequ?ncia menor apenas do que aquelas observadas para o
c?ncer de pele n?o-melanoma e c?ncer de mama. O fator inibit?rio da migra??o de
macr?fagos (MIF) ? produzido por diferentes tipos de c?lulas e participa de uma cadeia
complexa de eventos que favorece o processo de carcinog?nese, sendo poss?vel
observar um alto n?vel de express?o em quase todos os tipos de c?ncer humano, entre
eles o c?ncer do colo do ?tero. O MIF tamb?m induz um aumento dose dependente
na excre??o do fator de crescimento de endot?lio vascular (VEGF), que promove a
angiog?nese, o crescimento tumoral e a migra??o dessas c?lulas no c?ncer do colo
do ?tero. O objetivo deste estudo foi investigar a express?o de MIF e VEGF em
pacientes que apresentam ou n?o les?es do colo do ?tero, no intuito de identificar a
exist?ncia de uma rela??o direta entre a presen?a dos marcadores MIF e VEGF com
o grau da les?o da paciente, bem como, com a presen?a do papilomav?rus humano
(HPV). Foram inclu?das no estudo 45 mulheres que haviam sido encaminhadas para
a Maternidade Escola Janu?rio Cicco com suspeita de les?es na c?rvice uterina. As
pacientes que aceitaram participar do estudo responderam a um question?rio, para
obten??o de dados s?cio-demograficos, seguido de exame cl?nico. Das mulheres que
se submeteram a colposcopia, foram coletados dois fragmentos de tecido do colo do
?tero, um para an?lise histopatol?gica e outro para detec??o do HPV por PCR
convencional. A express?o dos biomarcadores, MIF e VEGF, foi detectada pela
t?cnica de imuno-histoqu?mica. A ?rea positiva de cada biomarcador foi lida e
quantificada utilizando o programa ImageJ?, e o resultado foi analisado atrav?s dos
programas de estat?stica SPSS? Statistics e GraphPad Prism. Das 45 pacientes
inclu?das no estudo, 20% apresentaram resultado do exame dentro dos padr?es da
normalidade, enquanto que 80% apresentaram algum tipo de altera??o no exame;
sendo 35,55% les?es intraepiteliais de baixo grau (LIEBG) e 44,44% les?es
intraepiteliais de alto grau (LIEAG). A taxa de preval?ncia global de infec??o genital
pelo HPV foi de 80%, sendo 86,1% em pacientes com les?o. A express?o m?dia do
VEGF e do MIF tiveram um aumento gradativo quando comparado entre as pacientes
normais, LIEBG e LIEAG, correspondendo respectivamente aos seguintes valores,
19,62, 41,59 e 55,42 para o VEGF e 4,36, 9,44 e 22,86 para MIF. Foi poss?vel verificar
uma correla??o positiva entre a express?o de MIF e VEGF (r = 0,523, p = <0,001).
Por meio deste trabalho p?de-se concluir que o VEGF e o MIF est?o correlacionados
e envolvidos no processo de displasia cervical, aumentando a sua express?o
conforme ocorre a progress?o da les?o. No entanto, n?o foi poss?vel encontrar
associa??o entre a presen?a do HPV e os n?veis de MIF e VEGF. / The cervical cancer is the third most frequent type of cancer in women in Brazil, and
its frequency is only lower than those observed for non-melanoma skin cancer and
breast cancer. Macrophage migration inhibitory factor (MIF) is produced by different
cell types and participates in a complex chain of events that favors the process of
carcinogenesis, being possible to observe a high expression level in almost all types
of human cancer, such as in cervical cancer. MIF also induces a dose-dependent
increase in the excretion of vascular endothelial growth factor (VEGF), which promotes
angiogenesis, tumor growth and migration of these cells in cervical cancer. The
objective of this study was to investigate the expression of MIF and VEGF in patients
with or without cervical lesions, in order to identify the existence of a direct relationship
between the presence of those markers with the degree of the lesion of the patient, as
well, as with the presence of human papillomavirus (HPV). The study included 45
women who had been referred to the Maternity School Janu?rio Cicco with suspected
lesions of the uterine cervix. Patients who accepted to participate in the study
answered a questionnaire to obtain socio-demographic data, followed by clinical
examination. Patients who agreed to participate in the study answered a questionnaire
to obtain socio-demographic data, followed by clinical examination. Two cervix tissue
fragments were collected from the women who underwent colposcopy, one for
histopathological analysis and the other for HPV detection by conventional PCR. The
expression of the biomarkers, MIF and VEGF, was detected by the
immunohistochemical technique. The positive area of each biomarker was read and
quantified using the ImageJ? program and the result was analyzed using the statistical
program SPSS? Statistics and GraphPad Prism. Of the 45 patients included in the
study, 20% showed no lesions, while 80% had some type of alteration in the exam;
being 35.55% low-grade squamous intraepithelial lesion (LSIL) type and 44.44% highgrade
squamous intraepithelial lesion (HSIL) type. The overall prevalence rate of
genital HPV infection was 80%, with 86.1% in patients with lesions. Mean VEGF and
MIF expression increased gradually when compared to normal patients, LSIL and
HSIL, respectively, corresponding to the following values: 19.62, 41.59 and 55.42 for
VEGF and 4.36, 9, 44 and 22.86 for MIF. A positive correlation between MIF and VEGF
expression was found (r = 0.523, p = <0.001). Through this work it was concluded that
VEGF and MIF are correlated and involved in the cervical dysplasia process,
increasing its expression as the lesion progresses. However, it was not possible to find
an association between the presence of HPV and the levels of MIF and VEGF.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24106 |
| Date | 31 May 2017 |
| Creators | Nobre, Camila Cristina Guimar?es |
| Contributors | 09457135415, Ara?jo, Joselio Maria Galv?o de, 01016476469, Cobucci, Ricardo Ney Oliveira, 73728322091, Fernandes, Thales Allyrio Ara?jo de Medeiros, 02751517439, Fernandes, Jos? Ver?ssimo |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM BIOLOGIA PARASIT?RIA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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