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Previous issue date: 2017-12-12 / Estudos sobre a influ?ncia do c?lon na regenera??o hep?tica s?o escassos. Alguns
t?m demonstrado que ap?s a colectomia ocorre aumento da regenera??o e sua
redu??o em outros. Diante da controv?rsia, este trabalho visou examinar se a
colectomia associada ? hepatectomia 70% influencia par?metros de regenera??o do
f?gado em ratos. Foram utilizados 18 ratos Wistar distribu?dos em 3 grupos (6
animais cada). No grupo I (sham) foi realizada uma laparotomia com leve
manipula??o de al?as; no grupo II uma colectomia + hepatectomia 70%; e no grupo
III apenas hepatectomia 70%. No 6? dia p?s-operat?rio foi colhido sangue por
pun??o card?aca sob anestesia, seguido de eutan?sia. Realizadas dosagens s?ricas
de alanina aminotransferase (ALT), aspartato amino transferase (AST), fosfatase
alcalina (FA), albumina, fator de crescimento de hepat?citos (HGF) e fator de
crescimento transformador-? (TGF-?). A regenera??o do f?gado foi calculada pela
f?rmula: (raz?o do peso do f?gado por 100g do peso corporal no momento da
eutan?sia/peso do f?gado no pr?-operat?rio projetado por 100g de peso corporal) ?
100. Os testes de an?lise de vari?ncia (ANOVA) e Tukey foram usados, com
signific?ncia p<0,05. Resultados: Houve uma menor e significante magnitude dos
n?veis de ALT e AST no grupo II comparados com o grupo III (p<0,001). A
albuminemia mostrou n?veis significativamente mais elevados no grupo II que no III.
Os n?veis de HGF (408?18,2 pg/mL) e TGF-? (3,8?0,3 ng/mL) no grupo II foram
significativamente mais elevados que o HGF (360?58,6 pg/mL) e TGF-? (2,3?0,4
ng/mL) no grupo III, respectivamente (p<0,001). O percentual de regenera??o
hep?tica foi significativamente mais elevado no grupo II, do que no grupo III
(p=0,003). Em conclus?o, este estudo concluiu que a colectomia realizada
simultaneamente ? hepatectomia 70% influenciou positivamente na regenera??o do
f?gado em ratos. Pesquisas adicionais s?o necess?rias para revelar os mecanismos
moleculares deste efeito e para caracterizar a influ?ncia do c?lon na fisiologia do
f?gado. / Studies on the influence of the colon on liver regeneration are scarce. Some have
shown that after colectomy there is an increase in regeneration and its reduction in
others. Faced with the controversy, this work aimed to investigate whether colectomy
simultaneously performed with 70% hepatectomy influences liver regeneration in
rats. Eighteen Wistar rats were distributed in 3 groups, 6 animals each. In group I
(sham) only laparotomy was performed with mild manipulation of boweld loops; in
group II 70% hepatectomy and simultaneous colectomy; in group III only 70%
hepatectomy. On the 6th postoperative day, blood was collected by cardiac puncture
under anesthesia, and remaining liver was removed. Serum measures of alanine
aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(FA), albumin, hepatocyte growth factor (HGF) and transforming growth factor-?
(TGF-?) were performed. Liver regeneration was calculated by (liver weight per 100g
of the body weight at sacrifice/preoperative projected liver weight per 100g of the
body weight) ? 100. The Analyses of variance (ANOVA) and Tukey tests were used,
with significance p<0.05. There was significant lower levels of ALT and AST in group
II rats when compared to group III (p<0.001). Albuminemia showed significantly
higher levels in group II rats than in III (p<0.01). Serum levels of HGF
(408?18.2pg/mL) and TGF-? (3.8?0.3ng/mL) were significantly higher in group II rats
than HGF (360?58.6pg/mL), TGF-? (2.3?0.4ng/mL) in group III, respectively. The
percentage of liver regeneration was significantly higher in group II than in group III
(p = 0.003). In conclusion, this study demonstrated that colectomy, performed
simultaneously with 70% hepatectomy, positively influenced liver regeneration in rats.
Further research is needed to reveal the molecular mechanisms of this effect and to
characterize the effects of the colon on liver physiology.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24785 |
| Date | 12 December 2017 |
| Creators | Moreira, Marilia Daniela Ferreira de Carvalho |
| Contributors | 07117701404, Formiga, Cipriano da Cruz, 02719795429, Padilha, Deborah de Melo Magalh?es, 93741162353, Cavalcanti J?nior, Geraldo Barroso, 10861203453, Sales, Val?ria Soraya de Farias, 25069144472, Medeiros, Aldo da Cunha |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS DA SA?DE, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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