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000774986.pdf: 9422731 bytes, checksum: e40055dff4f7f745ade353914c6e7948 (MD5) / Os produtos naturais têm desempenhado um papel importante pela humanidade, no uso da medicina popular e na geração da descoberta de novos fármacos. Fungos endofíticos são micro-organismos que vivem em associação mutualística com a espécie hospedeira trazendo benefícios a ambos, e são uma promissora fonte de produtos naturais. A espécie vegetal Eugenia jambolana tem sido intensamente explorada química e biologicamente devido a seus usos populares e por apresentar diversas atividades biológicas. No intuito de explorar a potencionalidade dessa espécie vegetal, E. jambolana foi submetida ao isolamento dos fungos endofíticos presentes nas folhas, caule e frutos, dos quais foram isolados treze fungos endofíticos. Estes endófitos foram cultivados em escala reduzida em Czapek (400 mL) para obtenção dos extratos brutos AcOEt. Os extratos brutos AcOEt foram submetidos a análises químicas (cromatografia em camada delgada comparativa, cromatografia líquida de alta eficiência e ressonância magnética nuclear de 1H) e biológicas (ensaios antioxidante, antifúngico, anticolinesterásico e citotóxico). Todos os extratos brutos apresentaram pelo menos uma atividade biológica positiva, o que adicionado as análises químicas permitiram selecionar dois fungos endofíticos para crescimento em escala ampliada, e isolamento dos metabólitos secundários. Destes, o fungo endofítico isolado do caule de E. jambolana codificado como Ej-c3 foi identificado como Saccharicola sp., foi possível isolar 7 substâncias das quais 4 são inéditas (4-7), os compostos 4 e 5 foram inativo nos ensaios realizados. Do estudo químico do fungo endofítico Botryosphaeria parva (Ej-f1), isolado das folhas, foram isolados 4 substâncias da classe das isocumarinas [meleina (3), 4-hidroximeleina (11), 5-hidroximeleina (13), 7-hidroximeleina (12)], as substâncias 3 e 13 foram ativas no ensaio antifúngico contra C. sphaerospermum. No intuito de aumentar a... / The natural products have occuped an important role for humanity, in the use of folk medicine and in the generation of drug discovery. Endophytic fungi are microorganism that living in a mutualist association with host species, refleting benefits to both, and they are a promising source for natural products. The vegetal species Eugenia jambolana has been intensively explored chemically and biologically, because of its popular uses and it has several biological activities. In order to explore the potencionality of this plant species, E. jambolana was submitted to isolation of endophytic fungi in the leaves stems and fruits, of which thirteen endophytic fungi were isolated. The endophytes were cultived in small scale in Czapek (400 mL) to give the EtOAc crude extract. The EtOAc crude extract were submited to chemistry (thin layer chromatography, high performace liquid chromatography and 1H nuclear magnetic ressonance) and biological (antioxidant, antifungal, anticholinesterase and cytotoxic activities) analysis. All of crude extract showed at least one positive biological activity, which added the chemical analyzes allowed us to select two endophytic fungi to growing on a larger scale, and isolation of secondary metabolites. The endophytic fungus isolated from stems of E. jambolana encoded Ej-c3 was identified as Saccharicola sp. it was isolated 7 substances of which 4 are new (4-7). The substances 4 e 5 were inactive in the biological assays. Chemical study of Botryosphaeria parva (Ej-f1) isolated from leaves, was isolated 4 isocoumains [melein (3), 4-hidroxymelein (11), 5-hidroxymelein (13), 7-hidroxymelein (12)]. The substances 3 and 13 were active in antifungal assay agains the fungus C. sphaerospermum. In order to increase the metabolite production and activate the silent biossintetic pathway, the epigenetic appear as a useful and simple tool to gene expression. Lecythophora sp. an endolichenic fungus from Parmotrema tinctorum, was...
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/110858 |
| Date | 26 February 2014 |
| Creators | Chapla, Vanessa Mara [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Araújo, Angela Regina [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 251 f. : il. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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