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Previous issue date: 2015-03-13 / In the present work, analyses were performed using leaf extracts constituted of the main
photosynthetics pigments of the plant Vicia faba, from which it was possible to isolate the
chlorophyll and pheophytin by separation column chromatographic method, which were
characterized by optical techniques. Additionally, the interactions were valued between the
molecules porphyrin (Chlorophyll and pheophytin) and nanoparticles of gold (AuNP) of 5, 10 and 20 nm of diameter, from the analyses of Absorption UV-Vis, Molecular fluorescence and time of life of luminescence. One checked that the molecules porphyrins presented reduction or increase in the fluorescence intensity, in accordance with the studied conditions, besides the reduction in the times of life of fluorescence of the molecules in the presence of the AuNPs. The effects were significantly more evident for the samples consisting of Pheophytin, suggesting a less affinity of the Chlorophyll to interact with the AuNPs, when compared with the porphyrin uncomplexed. Generally, it was determined that the AuNPs have great potential to interfere with the plant photosynthesis process, given the ability to interact strongly with the photosynthetic pigments that are critical in chemical production process in plants, highlighting a potential risk of toxicity the metal nanoparticles may have on the environment. / No presente trabalho foram obtidos extratos foliares constituídos dos principais pigmentos fotossintéticos da planta Vicia faba, a partir do qual pôde-se isolar a Clorofila e a Feofitina pelo método de separação em coluna cromatográfica, que foram caracterizados através das técnicas ópticas. Adicionalmente, foram avaliados as interações entre as moléculas porfirínicas (Clorofila e Feofitina) e nanopartículas de ouro (AuNP) de 5, 10 e 20 nm de diâmetro, a partir das análises de Absorção UV-Vis, Fluorescência Molecular e tempo de vida de luminescência. Verificou-se que as moléculas porfirínicas apresentaram diminuição ou
aumento na intensidade de fluorescência, de acordo com as condições estudadas, além da diminuição nos tempos de vida de fluorescência das moléculas na presença das AuNPs. Os efeitos das interações foram mais evidentes para as amostras constituídas de Feofitina, sugerindo uma menor afinidade da Clorofila para interagir com as AuNPs, quando comparado com a porfirina desmetalada. De forma geral, foi possível determinar que as AuNPs possuem grande potencial de interferir no processo de fotossíntese dos vegetais, dado a capacidade de interagir fortemente com os pigmentos fotossintéticos que são fundamentais no processo de produção química pelas plantas, ressaltando um potencial risco de toxicidade que as
nanopartículas metálicas podem apresentar ao meio ambiente.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede.ufgd.edu.br:tede/315 |
| Date | 13 March 2015 |
| Creators | Mezacasa, Adalberto Villalba |
| Contributors | Caires, Anderson Rodrigues Lima, Domingues, Nelson Luis de Campos, Nascimento, Valter Aragão do |
| Publisher | Universidade Federal da Grande Dourados, Química - Mestrado, UFGD, Brasil, FACET |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFGD, instname:Universidade Federal da Grande Dourados, instacron:UFGD |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
| Relation | 3119929381378724512, 500, 500, 600, 8816595718120871258, 1571700325303117195 |
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