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Previous issue date: 2014-01-14 / Oral cancer is among the six most common types of cancer worldwide, considered as the seventh most frequent in the Brazil. Tumor angiogenesis has become a promising target for the treatment of malignancies. Methods that allow three-dimensional evaluation of the vascular network of tumors have a great importance to assess the effects of antiangiogenic treatments in tumors. The objective of this study was to evaluate the effect of sunitinib maleate and the metronomic chemotherapy with cyclophosphamide on the vascular network of the oral squamous cell carcinoma by scanning electron microscopy (SEM) of corrosion casts. 24 Syrian hamsters (Mesocricetus auratus) were divided into three groups of eight animals each and had their right buccal pouches submitted to tumor induction with dimethylbenzanthracene and carbamide peroxide for 55 days. Following, the animals were maintained without treatment (group I) or receiving sunitinib (group II), or cyclophosphamide (group III) for a period of 4 weeks. After the treatment, six animals of each group had their vascular networks casted by Mercox? resin and analyzed qualitatively and quantitatively by SEM. The two remaining animals of each group had their buccal pouches prepared for qualitative analysis by light microscopy. It was observed changes in the angioarchitecture of the pouches that were treated with these two drugs, suggesting an improvement in the tumors vascularization. The main changes caused by the treatments were the reduction of vessel tortuosity, the reestablishment of vascular hierarchy, besides the decrease of sprouting and intussusception images, compressed vessels, saccular dilations, and other vascular figures. Quantitative analysis of the samples exhibited a statistically significant reduction in vascular diameter and intervascular distances ofthe pouches treated with cyclophosphamide, when compared with the pouches no treated and those treated with sunitinb. The findings of the present study suggest that these two drugs may act in the vascular normalization of squamous cell carcinomas induced in hamster buccal pouch, however the metronomic chemotherapy exhibited superior results. / O c?ncer oral est? entre os seis tipos mais comuns de c?ncer no mundo, sendo o s?timo mais encontrado entre a popula??o brasileira. O processo de angiog?nese tumoral tem-se tornado um alvo promissor para o tratamento de neoplasias malignas. Os m?todos que permitem a avalia??o tridimensional da angioarquitetura dos tumores s?o de grande import?ncia na an?lise dos efeitos de tratamentos antiangiog?nico. Este trabalho visou avaliar o efeito do maleato de sunitinibe e da quimioterapia metron?mica com ciclofosfamida na vasculatura do carcinoma oral de c?lulas escamosas, atrav?s de microscopia eletr?nica de varredura (MEV) de r?plicas vasculares. Assim, 24 hamsters s?rios (Mesocricetus auratus) divididos em tr?s grupos de oito animais cada, tiveram suas bolsas jugais direitas submetidas ? indu??o tumoral com dimetilbenzantraceno e per?xido de carbamida por 55 dias, sendo mantidos por mais 4 semanas sem tratamento (grupo I), ou recebendo sunitinibe (grupo II), ou ciclofosfamida (grupo III). Ap?s o tratamento, seis animais de cada grupo tiveram suas redes vasculares moldadas pela resina Mercox? e analisadas qualitativa e quantitativamente em MEV. Os dois animais restantes de cada grupo tiveram suas bolsas qualitativamente analisadas em microscopia de luz. Foram observadas modifica??es na angioarquitetura das bolsas que receberam tratamento com os dois f?rmacos, o que gerou uma normaliza??o da vasculariza??o dos tumores. As principais modifica??es provocadas pelos tratamentos foram a redu??o da tortuosidade dos vasos, o reestabelecimento da hierarquia vascular, al?m da diminui??o de imagens de sprouting e intussuscep??es, vasos comprimidos, dilata??es saculares e outras figuras vasculares. A an?lise quantitativa das amostras evidenciou uma redu??o estatisticamente significativa do di?metro vascular e das dist?ncias intervasculares nas bolsas tratadas com ciclofosfamida, quando comparadas com as bolsas que n?o receberam tratamento e com aquelas tratadas com sunitinibe. Os achados do presente estudo sugerem que essas duas drogas podem atuar na normaliza??o da rede vascular no carcinoma de c?lulas escamosas induzido em bolsa jugal de hamster; no entanto a quimioterapia metron?mica apresentou resultados superiores.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1779 |
| Date | 14 January 2014 |
| Creators | Bampi, Vin?cius Faccin |
| Contributors | Silva, Jefferson Luis Braga da |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 600, -8624664729441623247 |
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