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Previous issue date: 2017-08-11 / Glyphosate has become the most widely used herbicide in the world, due to the wide
scale adoption of resistant crops, after its introduction in 1996. Glyphosate can be used
alone, but is commonly used as an active ingredient of the Roundup? herbicide. This
herbicide contains several adjuvants in addition to glyphosate, which may promote an
unknown e.g. toxicity Polioxietilenamida (POEA). Zebrafish is gaining popularity in
behavioral research, because of its physiological similarity to mammals, ease of
manipulation, robust performance, low cost, external fertilization, transparency of
embryos larval stages and rapid development. The aim of this study was to evaluate the
effects of glyphosate and Roundup? on behavioral and morphological parameters in
zebrafish larvae and adult. Zebrafish larvae at 3 days post-fertilization (dpf) and adults
were exposed to glyphosate (0.01, 0.065, and 0.5 mg/L) and Roundup? (0.01, 0.065,
and 0.5 mg/L) for 96 hours. Immediately after the treatment, behavioral parameters such
as locomotor activity and aversive behavior and morphology for the larvae and
locomotion, agressive behavior and memory for the adults were analyzed. Zebrafish
larvae, the results indicated that there were significant differences in the locomotor
activity and aversive behavior by glyphosate and Roundup? when compared to the
control. However, there was a decrease in distance traveled and the time spent in zone
without stimulation for exposed larvae at doses of glyphosate and Roundup?. A
significant decrease in body lenght was observed for larvae exposed to Roundup? in all
concentrations tested. Our findings demonstrated that glyphosate and Roundup?
exposure reduced the distance traveled, the mean speed and the line crossings in the
highest concentration of glyphosate (0.5 mg / L) and 0.065 and 0.5mg/L Roundup? in
animals adults. We verified that Roundup?-treated adult zebrafish showed a significant
impairment in memory. Our results showed that glyphosate and Roundup? had an effect
on agressive behavior. Our findings demonstrated that the effects of isolated and
commercial forms of glyphosate promoted differences on locomotion, behavior and
morphology of the treated animal, suggesting similar mechanisms of toxicity and
cellular response. / O glifosato tornou-se o herbicida mais utilizado no mundo, devido ? ado??o ampla de
culturas resistentes, ap?s sua introdu??o em 1996. O glifosato pode ser usado sozinho,
mas ? comumente utilizado como ingrediente ativo do herbicida Roundup?. Este
herbicida cont?m v?rios adjuvantes, tal como a Polioxietilenamida (POEA), que podem
promover uma toxicidade desconhecida. O peixe-zebra est? ganhando popularidade na
pesquisa comportamental, devido ? similaridade fisiol?gica com os mam?feros,
facilidade de manipula??o, baixo custo, fertiliza??o externa, transpar?ncia de embri?es
nos est?gios larvais e desenvolvimento r?pido. O objetivo deste estudo foi avaliar os
efeitos do glifosato e do Roundup? sobre par?metros comportamentais e morfol?gicos
em peixe-zebra no est?gio larval e adulto. As larvas com 3 dias p?s-fertiliza??o (dpf) e
adultos foram expostos ao glifosato (0,01, 0,065 e 0,5 mg/L) e Roundup? (0,01, 0,065 e
0,5 mg/L) por 96 horas. Imediatamente ap?s o tratamento, realizamos a an?lise de
par?metros comportamentais, como atividade locomotora, comportamento aversivo e
morfologia para larvas e locomo??o, comportamento agressivo e mem?ria aversiva para
adultos. Nas larvas houveram diferen?as significativas na atividade locomotora e
comportamento aversivo nos animais tratados com glifosato e Roundup? quando
comparado ao controle. Foi observada uma diminui??o na dist?ncia percorrida e na
resposta aversiva nas larvas expostas ao glifosato e Roundup?. Observou-se uma
diminui??o significativa no comprimento corporal das larvas expostas ao Roundup? em
todas as concentra??es testadas. Nossos resultados demonstraram que a exposi??o ao
glifosato ou Roundup? reduziu a dist?ncia percorrida, a velocidade m?dia e o n?mero
de cruzamentos na maior concentra??o de glifosato (0,5mg/L) e 0,065 e 0,5mg/L de
Roundup? em animais adultos. Verificamos que peixe-zebra adulto tratado com
Roundup? apresentou um comprometimento significativo na mem?ria. Nossos
resultados demostraram que o glifosato e o Roundup? tiveram efeito sobre o
comportamento agressivo. Assim, nossos achados demonstraram que os efeitos das
formas isoladas e comerciais de glifosato promoveram diferen?as na locomo??o,
comportamento e morfologia do animal tratado, sugerindo mecanismos semelhantes de
toxicidade e resposta celular.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7700 |
| Date | 11 August 2017 |
| Creators | Bridi, Daiane |
| Contributors | Bonan, Carla Denise |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biotecnologia Farmac?utica, PUCRS, Brasil, Faculdade de Farm?cia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2304961219518893267, 500, 500, 500, -8380654636843378116, 6997636413449754996 |
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