Objetivo: Evaluar la actividad antibacteriana de tres ionómeros de vidrio (CIVc) Ion Z®, Ketac Molar Easymix® y Fuji IX GP® sobre Streptococcus mutans (ATCC 35668) y Lactobacillus acidophilus (ATCC 4366). Materiales y métodos: El presente estudio fue experimental in vitro. Se realizó mediante el método de contacto directo y densidad óptica con una microplaca, donde se utilizó seis pocillos por cada ionómero de vidrio. Posteriormente, se adicionaron 150 ul de Streptococcus mutans y Lactobacillus acidophilus de manera independiente en agar BHI. Las muestras fueron incubadas a 37 °C en condiciones de anaerobiosis controlada. En el caso de la viabilidad bacteriana se utilizó el ensayo de MTT. Resultados: Se encontró que el efecto antibacterianode Ketac Molar Easymix® frente a la cepa de S. mutans tuvo 0.06 ± 0.01, mientras que Ion Z® 0.16 ± 0.03 ante la cepa de L. acidophilus, presentando diferencias estadísticamente significativas. Con respecto a la viabilidad bacteriana de S. mutans frente al Ketac Molar Easymix® obtuvo 0.92 ± 0.02, lo que corresponde a 29 % de bacterias viables, mientras que, L. acidophilus frente al Ion Z® tuvo 0.31± 16, que representa 19% de bacterias viables. Conclusiones: Los tres CIVs mostraron actividad antibacteriana, en el caso de Ketac Molar Easymix® demostró mayor efecto antibacteriano ante S. mutans, por otro lado, se evidenció que Ion Z® fue más eficaz frente L. acidophilus. / Objective: To evaluate the antibacterial activity of three glass ionomers cements (Ion Z®, Ketac Molar Easymix® and Fuji IX GP®) on Streptococcus mutans (ATCC 35668) and Lactobacillus acidophilus (ATCC 4366). Materials and methods: The present study was experimental in vitro. It was made out using the direct contact method and optical density with a microplate, using six wells for each glass ionomer cement. Later, 150 ul of Streptococcus mutans and Lactobacillus acidophilus were independently added on BHI agar. The samples were incubated at 37 °C under conditions of controlled anaerobiosis. In the case of bacterial viability, the MTT assay was used. Results: It was found that the antibacterial effect of Ketac Molar Easymix® against the strain of Streptococcus mutans had 0.06 ± 0.01, while in Z® it was 0.16 ± 0.03 before the strain of Lactobacillus acidophilus, presenting statistically significant differences. Regarding the bacterial viability of Streptococcus mutans against Ketac Molar Easymix® obtained 0.92 ± 0.02, what corresponds to 29% of viable bacteria, whereas Lactobacillus acidophilus against Ion Z® had 0.31± 16, which represents 19% of viable bacteria. Conclusions: The three glass ionomers cements showed antibacterial activity. Ketac Molar Easymix® demonstrated greater antibacterial effect against Streptococcus mutans; on the other hand, it was evidenced that Ion Z® was more effective against Lactobacillus acidophilus. / Tesis
| Identifer | oai:union.ndltd.org:PERUUPC/oai:repositorioacademico.upc.edu.pe:10757/625106 |
| Date | 04 December 2018 |
| Creators | Gil Barreda, Arlet Angelita, García Calero, Olga Beatriz |
| Contributors | Del Valle Mendoza, Juana Mercedes, Flores Barrantes, Lorena |
| Publisher | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Source Sets | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Language | Spanish |
| Detected Language | English |
| Type | info:eu-repo/semantics/bachelorThesis |
| Format | application/pdf, application/epub, application/msword |
| Source | Universidad Peruana de Ciencias Aplicadas (UPC), Repositorio Académico - UPC |
| Rights | info:eu-repo/semantics/openAccess, Attribution-NonCommercial-ShareAlike 3.0 United States, http://creativecommons.org/licenses/by-nc-sa/3.0/us/ |
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