Made available in DSpace on 2014-06-11T19:28:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-12-01Bitstream added on 2014-06-13T18:32:31Z : No. of bitstreams: 1 nassar_po_me_arafo.pdf: 1193015 bytes, checksum: 40452517ded346fcde49af240f22a5a6 (MD5) / A administração da CsA tem sido associada a osteopenia ou aumento na remodelação óssea. O objetivo do estudo foi avaliar o efeito da CsA no processo alveolar de ratos com doença periodontal induzida, por meio de análises sérica, estereométrica, histológica e histométrica. 24 ratos foram divididos em 4 grupos de 6 animais cada: Grupo 1: Controle; Grupo 2: ligadura ao redor do 1° molar inferior; Grupo 3: ligadura ao redor do 1° molar inferior e tratamento com CsA a 10mg/Kg de peso corporal/dia; Grupo 4: tratamento com CsA a 10mg/Kg de peso corporal/dia. Após 30 dias os animais foram sacrificados e os níveis séricos de cálcio e fosfatase alcalina foram mensurados. Após análise histológica, uma análise histométrica da junção cemento-esmalte até a crista óssea alveolar, da área de tecido ósseo e de reabsorção, mais espessura de cemento foi realizada conjuntamente com os parâmetros estereológicos: densidade volumétrica de osteoclastos multinucleados (Vo), osso alveolar (V-osso), medula (V-medula) e relação de superfície reabsorvida/superfície óssea (Sr/So) foram analisados na região mesial do osso alveolar. Diminuição significante foi observada no cálcio sérico nestes grupos que receberam terapia com CsA. Não foi observada mudança significante na fosfatase alcalina sérica. A terapia com CsA combinada com ligadura diminuiu o V-osso e aumentou o Vo, V-medula e Sr/So na superfície mesial dos primeiros molares inferiores, dados esses concordantes com a análise histométrica e comprovada pela leitura histológica. Além disso a espessura de cemento foi aumentada nos grupos tratados com CsA em relação aos não tratados. Entretanto, apesar da falta de efeitos diretos na altura do osso alveolar, a terapia com CsA intensifica o desequilíbrio da homeostasia do osso alveolar em ratos com doença periodontal induzida. / The administration of CsA has been associated with significant bone loss and increased bone remodeling. The present investigation was designed to evaluate the effects of CsA on alveolar bone of rats subjected to experimental periodontitis, using serum, stereometric and histological analysis. 24 rats were divided in groups 6 animal each: 1-control; 2-rats with ligature around the lower 1st.molars; 3-rats with ligature around the lower 1st.molars and were treated with 10mg/kg body weight/day CsA; 4-rats were treated with 10mg/kg body weight/day CsA. At the end 30 days, rats were sacrificed and calcium and alkaline phosphatase levels were measured. After histological processing, the histomorfometric analysis of the distance cemento-enamel junction and alveolar bone crest, bone area, eroded bone area and cemento surface together with stereological parameters: volume densities of multinucleated osteoclasts(Vo), alveolar bone(V-bone), marrow(V-marrow), and relation of eroded surface/bone surface(Es/Bs) were assessed at the mesial region of the alveolar bone. Significant decreases in serum calcium were observed in groups with CsA therapy. No significant changes in serum alkaline phosphatase were observed. The CsA therapy combined with ligature placement decreased the V-bone and increased the Vo, V-marrow and Es/Bs at the mesial surface and the results at the histologic and histomorfometric analysis showed the same combination and changes. Significant increase on the cemento height were observed in the CsA therapy groups. Therefore, in spite of the lack of a direct effect on the alveolar bone height, the CsA therapy intensified the imbalance of the alveolar bone homeostasia in a rat model of experimental periodontitis.

Objetivo: Comparar in vitro la rugosidad superficial de 2 resinas Bulk Fill utilizando dos sistemas de pulido de cauchos abrasivos: JOTA® (Suiza) (oxido de aluminio, carburo de silicio y silicona) y Optimize® (TDV) (Alemania) (silicona-oxido de aluminio). Materiales y métodos: Se utilizaron 60 especímenes de resina divididos en cuatro grupos G1: Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, Estados Unidos)/ sistema de pulido JOTA®(Suiza), G2: Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, Estados Unidos)/sistema de pulido Optimize® (TDV) (Alemania), G3: Aura Bulk Fill (SDI, Brasil) / sistema de pulido JOTA® (Suiza) y G4: Aura Bulk Fill (SDI, Brasil) /sistema de pulido Optimize® (TDV) (Alemania). Todos los especímenes fueron confeccionados con una matriz metálica (6mm x 4mm), donde se insertó las resinas con una espátula de resina (TNCIGFT2 Hu Friedy), y se fotoactivó con la lámpara Elipar LedTM (3M-ESPE), con una intensidad 1200 mW/cm2. Inmediatamente, se midió la rugosidad inicial de todos los grupos con el rugosímetro Mitutoyo SJ-210P (China). Posteriormente, se realizó los pulidos con los dos sistemas de cauchos según cada grupo, y se procedió a medir la rugosidad final. Resultados: Se encontró diferencia estadísticamente significativa en la rugosidad del grupo G1. Esta diferencia es mayor a la de los demás grupos evaluados, y que la menor diferencia en la rugosidad fue la encontrada en el grupo G2. Asimismo, se encontró diferencia estadísticamente significativa en la comparación realizada entre las resinas evaluadas y los sistemas de pulido evaluados. Conclusiones: La rugosidad superficial encontrada en la evaluación de la resina Bulk Fill Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, Estados Unidos), con el sistema de pulido JOTA® (Suiza) presenta una menor rugosidad que la resina Aura Bulk Fill (SDI, Brasil) con los dos sistemas de pulido evaluados. / Objective: Comparison in vitro the superficial roughness of two Bulk Fill resins using two polishing systems of abrasives rubbers: JOTA® (Switzerland) (aluminum oxide, silicium carbide and silicone) and Optimize® (TDV) (Germany) (silicone- aluminum oxide). Materials and methods: Were used 60 resin specimens of resin divided in four groups G1: Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, USA)/ polishing system JOTA®( Switzerland), G2: Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, USA)/polishing system Optimize® (TDV) (Germany), G3: Aura Bulk Fill (SDI, Brazil) / polishing system JOTA® (Switzerland) y G4: Aura Bulk Fill (SDI, Brazil) / polishing system Optimize® (TDV) (Germany). All the specimens were made in a metal matrix (6mm x 4mm), were the resins were inserted with a resin spatula (TNCIGFT2 Hu Friedy), and fotoactivated with a lamp Elipar LedTM (3M-ESPE), with an intensity of 1200 mW/cm2. Immediately, the initial roughness of all groups is measured with the Roughness meter Mitutoyo SJ-210P (China). Subsequently, the polishing was executed out with the two rubber systems according to each group, and the final roughness was measured. Results: Statistically significant difference was found on the roughness of G1. This is even greater than all the groups evaluated, and that the smallest difference was in the G2 group. A statistically significant difference was found when comparing the two resins with the polishing systems evaluated. Conclusions: The surface roughness found in the evaluation of Bulk Fill Tetric-N Ceram® Bulk Fill (Ivoclar Vivadent, USA), with the JOTA® (Switzerland) polishing system presents the lower roughness than Aura Bulk Fill resin (SDI, Brazil) with the two polishing systems evaluated.

Objetivo: Comparar la microdureza y rugosidad superficial in vitro en resinas Bulk Fill después de un desafío abrasivo con pasta blanqueadora. Materiales y métodos: Noventa especímenes de resinas Bulk Fill Filtek™ Bulk Fill (FB), Tetric® N-Ceram Bulk Fill (TNC), Opus Bulk Fill (OBF) fueron divididos en 6 grupos (n=15). El desafío abrasivo in vitro fue realizado con un cepillo eléctrico Oral B y una solución de 3,3 ml de pasta dental / agua destilada por 30 s. La microdureza y rugosidad superficial inicial y final fue evaluada a los 15 y 30 días. Los datos fueron analizados mediante estadística descriptiva e inferencial (U Mann Whitney) Resultados: La rugosidad superficial a los 15 días fue: FB (0.09± 0.03µm/0.17± 0.10 µm); TNC (0.16 ± 0.09 µm/0.20± 0.09µm) y OBF (0.13±0.09 µm/0.18±0.06 µm). A los 30 días fue: FB (0.10 ± 0.05 µm/0.12 ±0.06 µm); TNC (0.07±0.04 µm/0.16±0.07µm) y OBF (0.11±0.03 µm/0.22±0.12 µm). Hubo diferencias estadísticamente significativas para FB (p=0.003) y OBF (p=0.0008) a los 15 días. A los 30 días, las resinas que presentaron un aumento significativo en los valores de rugosidad fueron OBF (p<0.0001) y TNC (p=0.0011). En la microdureza superficial, a los 15 días fue: FB (63.49±15.10 kg/mm2 / 65.53±8.21kg/mm2); TNC (47.67±6.20 kg/mm2 / 46.67±6.43 kg/mm2) y OBF (47.33±4.57 kg/mm2 / 45.4±4.32 kg/mm2). Existe una disminución a los 15 días en la resina OBF (p=0.0001). Conclusiones: Existe un aumento en la rugosidad superficial de las resinas Bulk Fill a los 15 y 30 días. Hubo una disminución en la microdureza superficial para la resina Opus Bulk Fill a los 15 días. Aim: To compare in vitro the microhardness and surface roughness in Bulk Fill composites after an abrasive challenge with whitening toothpaste. Materials and methods: Ninety specimens of Bulk Fill Filtek ™ Bulk Fill (FB), Tetric® N-Ceram Bulk Fill (TNC), Opus Bulk Fill (OBF) composite were divided into 6 groups (n = 15).The in vitro abrasive challenge was performed with an Oral B electric toothbrush and a 3,3 ml solution of toothpaste / distilled water for 30 s. The microhardness and initial and final surface roughness was evaluated at 15 and 30 days. The data were analyzed by descriptive and inferential statistics ¬(Mann Whitney U Test). Results: The surface roughness at 15 days was: FB (0.09 ± 0.03μm / 0.17 ± 0.10 μm); TNC (0.16 ± 0.09 μm / 0.20 ± 0.09μm) and OBF (0.13 ± 0.09 μm / 0.18 ± 0.06 μm). At 30 days it was: FB (0.10 ± 0.05 μm / 0.12 ± 0.06 μm); TNC (0.07 ± 0.04 μm / 0.16 ± 0.07μm) and OBF (0.11 ± 0.03 μm / 0.22 ± 0.12 μm). There were statistically significant differences for FB (p = 0.003) and OBF (p = 0.0008) at 15 days. After 30 days, the Bulk Fill composite that showed significant increases in the roughness values were OBF (p <0.0001) and TNC (p = 0.0011). The surface microhardness at 15 days was: FB (63.49 ± 15.10 kg / mm2 / 65.53 ± 8.21kg / mm2); TNC (47.67 ± 6.20 kg / mm2 / 46.67 ± 6.43 kg / mm2) and OBF (47.33 ± 4.57 kg / mm2 / 45.4 ± 4.32 kg / mm2). There was a decrease after 15 days in the OBF composite (p = 0.0001). Conclusions: There was an increase in the surface roughness of Bulk Fill composite at 15 and 30 days. There was a decrease in surface microhardness for the Opus Bulk Fill composite after 15 days. / Tesis

Objetivo: El objetivo del estudio es evaluar la resistencia a la tracción diametral y microdureza del cemento experimental y de materiales de restauración temporal utilizados en endodoncia (Óxido de zinc y Eugenol, Ionómero de vidrio convencional, Clip F® y Coltosol® F). Materiales y métodos: El estudio fue de tipo experimental in vitro. Se confeccionaron 11 muestras para cada cemento provisional, por cada propiedad a evaluar. Posteriormente, las muestras fueron almacenadas a temperatura ambiente por 24 h. Se midió la resistencia a la tracción diametral por compresión a través de la máquina de ensayo universal Instron® y la microdureza a través del Microdurómetro punta Vickers. Los resultados fueron analizados mediante estadística descriptiva univariada y para el análisis bivariado se realizó la prueba de Kruskal Wallis. Resultados: El cemento experimental obtuvo valores mayores de resistencia a la tracción diametral (0.77 MPa + 0.34) en comparación al Coltosol ® F (0.41 MPa + 0.13). Además, obtuvo mayores resultados de microdureza (9,45 HV + 0.66) en comparación al Coltosol ® F (6.50 HV + 0.61) y Óxido de zinc y Eugenol (5,62 HV + 0.61). Sin embargo, presentó menores valores de resistencia a la tracción diametral en comparación del ionómero de vidrio (24.46 + 4.55) y Clip F® (23.06 + 2.91). Asimismo, presento menores valores de microdureza comparado con el Ionómero de vidrio convencional (38.62 + 0.67) y Clip F® (26.03 + 1.07). En ambas propiedades se encontraron diferencias estadísticamente significativas (p<0.05). Conclusiones: El cemento experimental no demostró ser un buen material a comparación del Ionómero de vidrio convencional y el Clip F®. Sin embargo, obtuvo mejores valores que el Óxido de zinc y Eugenol, y Coltosol®; los cuales son empleados comúnmente en el ámbito odontológico a pesar de tener propiedades deficientes. / Objective: The objective of this study is to evaluate the diametral tensile strength and microhardness of an experimental cement and temporary restorative materials used in endodontics (Zinc Oxide and Eugenol, conventional glass ionomer, Clip F® and Coltosol® F). Material and methods: The sample of this in vitro study consisted in 11 samples were made from each provisional restorative materials, for each property to be evaluated. The samples were stored at room temperature for 24 h. The diametral tensile strength by compression was measured through the Instron® universal testing machine and the microhardness through the Vickers tip microdurometer. The results were analyzed using univariate descriptive statistics and the Kruskal Wallis test was performed for the bivariate analysis. Results: In this study, the experimental cement obtained higher values of diametral tensile strength (0.77 MPa + 0.34) in comparison of Coltosol® F (0.41 MPa + 0.13). In addition, it obtained higher microhardness results (9.45 HV + 0.66) in comparison of Coltosol ® F (6.50 HV + 0.61) and Zinc Oxide and Eugenol (5.62 HV + 0.61). However, lower diametral strength values were observed compared to glass ionomer (24.46 + 4.55) and Clip F® (23.06 + 2.91). Also, the microhardness values were lower compared to the conventional glass ionomer (38.62 + 0.67) and the Clip F® (26.03 + 1.07). In both properties, statistically significant differences were found (p <0.05). Conclusions: The experimental cement did not prove to be a good material compared to the conventional glass ionomer and the Clip F®. However, it obtained better results than Zinc Oxide and Eugenol, and Coltosol® F; which are commonly used in the dental field despite having poor properties. / Tesis

Objetivo: Comparar in vitro, la resistencia compresiva de cinco cementos ionómeros de vidrio: KetacTM Molar Easymix (3M ESPE), Gc Fuji IX GP, GC EQUIATM Fil (Gc corporation), Maxxion R (FGM), y KetacTM Universal HM (3M ESPE); tras 24 horas, 7 y 60 días de mezclado. Materiales y métodos: Se seleccionaron tres cementos de ionómero de vidrio de alta viscosidad: KetacTM Molar Easymix (3M ESPE), Gc Fuji IX GP, GC EQUIATM Fil (Gc corporation), y dos cementos de ionómero de vidrio convencionales: Maxxion R (FGM), KetacTM Universal HM (3M ESPE), indicados para el Tratamiento Restaurador Atraumático (TRA). La manipulación fue realizada en estricto cumplimiento con las indicaciones del fabricante. La unidad de análisis fue conformada por cilindros de ionómero de vidrio (2 mm de alto x 4 mm de diámetro), elaborados en una matriz metálica. Se crearon 15 especímenes por cada grupo de estudio, obteniendo un total de 225 especímenes. La prueba de resistencia a la compresión se dio mediante la máquina universal Instron® colocando los especímenes uno por uno verticalmente. La carga compresiva inicial tuvo una velocidad de 1mm/min hasta que la muestra se fracturase, y los resultados se registraron en megapascales (MPa). Resultados: El ionómero encapsulado GC EQUIATM Fil (Gc corporation) mostró mayores valores de resistencia compresiva a las 24 horas (299.80 MPa), mientras que a los 7 y 60 días fue el KetacTM Universal HM (3M ESPE), 77.04 MPa y 75.48 MPa, respectivamente. Por otro lado, existen diferencias significativas en la comparación de cada cemento de ionómero de vidrio en los tres tiempos de evaluación. Así como también, existen diferencias significativas en los tres tiempos evaluados: 24 horas: GC GC EQUIATM Fil (Gc corporation), a los 7 y 60 días KetacTM Universal HM (3M ESPE). Conclusión: El ionómero encapsulado GC EQUIATM Fil (Gc corporation) mostró mayores valores de resistencia compresiva a las 24 horas. Existen diferencias significativas en la comparación de cada cemento de ionómero de vidrio a lo largo de los tres tiempos, asimismo en cada tiempo de evaluación: 24 horas: GC EQUIATM Fil (Gc corporation), a los 7 y 60 días KetacTM Universal HM (3M ESPE). Objective: The aim of the present research was to compare in vitro the compressive strength of five glass ionomer cements: KetacTM Molar Easymix (3M ESPE), Gc Fuji IX GP, GC EQUIATM Fil (Gc corporation), Maxxion R (FGM), and KetacTM Universal HM (3M ESPE); after 24 hours, 7 days and 60 days after the mixing process. Materials and methods: Three high-viscosity glass ionomer cements were selected for the present in vitro study: KetacTM Molar Easymix (3M ESPE), Gc Fuji IX GP, GC EQUIATM Fil (Gc corporation), and also two conventional glass ionomer cements: Maxxion R (FGM), and KetacTM Universal HM (3M ESPE), recommended for the Atraumatic Restorative Treatment (ART). The mixing process was performed strictly respecting the manufacturer recommendations. The analysis unit was made of glass ionomer cylinders of 2mm high and 4mm in diameter, prepared with a metallic matrix. 15 samples were created for each study group, obtaining a total of 225 samples. The compressive strength was evaluated by Instron® universal machine placing the specimens one by one vertically, with a speed of 1mm / min until the sample fractured, and the results were registered in MPa. Results: The encapsulated ionomer GC EQUIATM Fil (Gc corporation) showed higher values of compressive strength at 24 hours (299.80 MPa), while at 7 and 60 days it was KetacTM Universal HM (3M ESPE), 77.04 MPa and 75.48 MPa, respectively. On the other hand, there are significant differences in the comparison of each glass ionomer cement in the three evaluation times. As well as, there are significant differences in the three times evaluated: 24 hours: GC EQUIATM Fil (Gc corporation), at 7 and 60 days KetacTM Universal HM (3M ESPE). Conclusion: The encapsulated ionomer GC EQUIATM Fil (Gc corporation) showed higher values of compressive strength at 24 hours. There are significant differences in the comparison of each glass ionomer cement over the three times, also at each evaluation time: 24 hours: GC EQUIATM Fil (Gc corporation), at 7 and 60 days KetacTM Universal HM (3M ESPE). / Tesis

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde. Programa de Pós-Graduação em Odontologia / Made available in DSpace on 2013-06-25T22:25:29Z (GMT). No. of bitstreams: 1 310428.pdf: 1725310 bytes, checksum: 9f49e9537954cd759ff4aac120cee16c (MD5) / O objetivo deste estudo foi avaliar a biocompatibilidade dos cimentos iRoot SP, MTA FILLAPEX, DiaRoot e MTA BRANCO através da análise da reação tecidual estimulada após a implantação de tubos de dentina preenchidos com os cimentos, em tecido subcutâneo de ratos. Foram utilizados 32 ratos, divididos em 4 grupos, de acordo com os materiais e períodos experimentais de 7, 15, 30 e 90 dias. Quatro tubos de dentina preenchidos com os cimentos e um quinto tubo, deixado vazio (controle), foram implantados no tecido subcutâneo de cada um dos ratos em 5 sítios, na região dorsal. Passados os períodos experimentais, foi realizada a eutanásia dos animais e os tubos e tecidos circundantes foram excisados em bloco. Após a fixação do tecido, os tubos de dentina foram cuidadosamente removidos e o tecido foi processado para inclusão em parafina. Foi realizada a coloração de Hematoxilina e Eosina para observação da morfologia tecidual, e os seguintes critérios foram avaliados: organização, composição e espessura da cápsula conjuntiva, intensidade da inflamação, presença de células gigantes multinucleadas (CGMNs), macrófagos, necrose tecidual e neovascularização. Também foi realizada a técnica de Von Kossa para avaliação de estruturas calcificadas, bem como análise em microscópio de luz polarizada, para observação de cristais de calcita. Os dados foram submetidos ao teste exato de Fischer e/ou Kruskal-Wallis a um nível de significância de 5%. Não foram observadas diferenças significativas entre os grupos em relação à intensidade da reação inflamatória, espessura e organização da cápsula conjuntiva nos diferentes períodos experimentais. Foram observadas diferenças significativas em relação à presença de CGMNs, macrófagos e necrose tecidual, sendo estas variáveis mais frequentes nos espécimes do MTA FILLAPEX e no iRoot SP. Em todos os grupos foi observada positividade ao Von Kossa, relacionada às áreas de necrose, porém, não foram detectados cristais de calcita. Todos os cimentos demonstraram biocompatibilidade, visto que as reações teciduais foram atenuando-se ao longo do tempo, exceto o MTA FILLAPEX que, aos 90 dias, ainda estimulou uma reação inflamatória moderada no tecido adjacente, necrose tecidual, presença de células gigantes multinucleadas e macrófagos fagocitando partículas do cimento. / The aim of this study was to evaluate the biocompatibility of the sealers iRoot SP, MTA FILLAPEX, DiaRoot BioAggregate and MTA BRANCO through the analysis of the tissue reaction stimulated by the implantation of dentin tubes filled with the sealers in the subcutaneous tissue of rats. We used 32 rats, divided into 4 groups of 8 animals, according to the materials and experimental periods of 7, 15, 30 and 90 days. Four dentin tubes, each filled with one of the experimental sealers and a fifth tube, left empty (control), were implanted in the subcutaneous tissue of each rat at 5 locations equidistant, in the dorsal area. After the experimental periods, the animals were submitted to euthanasia and the tubes with surrounding tissue were excised. After fixation, the dentin tubes were carefully removed and the surrounding tissue was processed for parafin inclusion. Staining was performed with Hematoxylin and Eosin to evaluate tissue morphology using the following criteria: organization, composition and thickness of the connective capsule, intensity of inflammation, presence of multinucleated giant cells, macrophages, tissue necrosis and neovascularization. Von Kossa staining was also performed as well as microscope evaluation under polarized light to observe calcite crystals. Data were analysed using Fisher#s exact test and the Kruskal-Wallis test at a significance level of 5%. There was no statistically significant difference among the scores of different groups regarding the intensity of the inflammatory reaction, thickness or organization of the connective capsule in the different experimental periods. Statiscally significant differences were observed between the groups regarding the presence of multinucleated giant cells, macrophages and necrosis, more visible in MTA FILLAPEX and iRoot SP. In all groups were observed Von Kossa positivity related to areas of necrosis but calcite crystals were not detected. All sealers were found to be biocompatible since the tissue reactions decreased during the experimental periods, except in the case of MTA FILLAPEX that, at 90 days, still stimulated a moderate inflammatory reaction in the adjacent tissue, tissue necrosis, presence of multinucleated giant cells and macrophages phagocyting particles of the sealer.

Made available in DSpace on 2014-06-11T19:23:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2004Bitstream added on 2014-06-13T19:09:42Z : No. of bitstreams: 1 esper_hr_me_araca.pdf: 903917 bytes, checksum: 93df3a7c105f3e9a41fb0a7b59b00e1e (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Um dos fatores importantes no sucesso do reimplante dentário tardio parece ser dependente do tipo de tratamento da superfície radicular. Esse tratamento inclui a remoção do ligamento periodontal cementário necrótico que pode favorecer o aparecimento das reabsorções radiculares externas, maior causa da perda dos dentes reimplantados nessas condições. O objetivo desse estudo foi comparar a efetividade de duas técnicas de remoção mecânica do ligamento periodontal cementário necrosado e a preservação da camada de cemento. Foram utilizadas 45 raízes de pré-molares hígidos extraídos com finalidade ortodôntica. Após a extração os dentes permaneceram por um período de uma hora em meio ambiente e passado esse tempo foram imersos em soro fisiológico por mais dez minutos. A partir desse passo foram divididos em 3 grupos experimentais. Grupo 1: controle, o ligamento periodontal cementário foi mantido. Grupo 2: remoção do ligamento periodontal cementário por meio de raspagem com lâmina de bisturi (RLB). Grupo 3: remoção do ligamento periodontal cementário por meio de escova de Robinson montada em baixa rotação e pasta de pedra pomes e água (RER). Após processamento das peças foram feitos cortes transversais e longitudinais nessas raízes para análise em microscopia óptica e eletrônica de varredura (MEV). Pela análise qualitativa e quantitativa dos resultados concluiu-se que a técnica RER foi mais eficiente que a RLB quanto à remoção do ligamento periodontal cementário, porém ambas preservaram a camada de cemento. / One of the important features related to the success of delayed tooth replantation seems to depend on the type of root surface treatment. This treatment encloses the removal of necrotic cemental periodontal ligament, which may favors the occurrence of external root resorptions that are the major cause of loss of teeth replanted in such conditions. The aim of this study was to compare the effectiveness of two techniques for mechanical removal of necrotic cemental periodontal ligament and the preservation of the cementum layer. Forty-five roots of sound premolars extracted for orthodontic purposes were selected. After extraction, the teeth were maintained at room ambient conditions for 1 hour and then immersed in saline for additional 10 minutes. Afterwards, the roots were assigned to three experimental groups. Group 1: control, the cemental periodontal ligament was maintained. Group 2: removal of the cemental periodontal ligament by scaling with a scalpel blade (SBR). Group 3: removal of the cemental periodontal ligament using a Robinson bristle brush, at low-speed, with pumice/water slurry (RBR). After processing of the pieces, the roots were sectioned transversally and longitudinally and the specimens obtained were examined under optical and scanning electron microscopy (SEM). The quantitative and qualitative analyses of the results showed that the RBR technique was more effective than the SBR technique, as regards the removal of the cemental periodontal ligament. Both techniques preserved the cementum layer.

Made available in DSpace on 2014-06-11T19:24:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-07-19Bitstream added on 2014-06-13T19:52:09Z : No. of bitstreams: 1 esberard_rr_me_arafo.pdf: 2831212 bytes, checksum: 853c7064958304ce94ed88b7ba2ae73f (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo desse estudo foi avaliar, em microscopia eletrônica de varredura, a morfologia do esmalte, da dentina, do cemento e, principalmente, da junção amelocementária humanos após o processo de clareação dentária, comparando-se a ação agressiva das diferentes técnicas de clareação e dos agentes clareadores sobre os tecidos que compõem a junção amelocementária. Quarenta e cinco dentes foram selecionados e seccionados ao meio, no sentido vestíbulo-lingual, de modo que obteve-se noventa espécimes. Assim, quarenta e cinco espécimes serviram de controle e seus pares foram submetidos às seguintes técnicas de clareação: Grupo I- clareação externa com peróxido de carbamida 10% (Opalescence); Grupo II- clareação externa com peróxido de hidrogênio 35% (Lase Peroxide); Grupo III- clareação externa com peróxido de hidrogênio 35% (Opalescence Xtra); Grupo IV- clareação interna/externa com peróxido de hidrogênio 35% (Lase Peroxide); Grupo V- clareação interna/externa com peróxido de hidrogênio 35% (Opalescence Xtra) e Grupo VI- clareação interna com pasta de perborato de sódio + peróxido de hidrogênio 30 V. Após as clareações, todos os espécimes, clareados ou não, foram avaliados em MEV. Os espécimes dos Grupos I, II e III foram avaliados externamente e os espécimes dos Grupos IV, V e VI foram avaliados interna e externamente. A análise microscópica mostrou que ocorreram alterações no esmalte, no cemento e na dentina de todos os espécimes clareados, porém, a junção amelocementária foi a parte mais afetada pelos agentes clareadores estudados, os quais promoveram mudanças no padrão da junção, aumentado a exposição da superfície dentinária e formando junções do tipo gaps ou fenestradas, exibindo extensas áreas de cemento separado do esmalte, sem cemento intermediário e com exposição dos túbulos dentinários. Aplicou-se o... . / The purpose of this study was to evaluate in SEM the effects of bleaching agents on the surface of human enamel, dentin, cementum and cementoenamal junction, comparing the action of the different techniques and bleaching agents used. Forty five teeth were selected and sectioned longitudinally so ninety specimens were obtained. Forty five specimens served as controls and the other ones were bleached by sixs protocols: Group I- external bleaching with 10% carbamide peroxide (Opalescence); Group II- external bleaching with 35% hydrogen peroxide (Lase Peroxide); Group III- external bleaching with 35% hydrogen peroxide (Opalescence Xtra); Group IV- internal/external bleaching with 35% hydrogen peroxide (Lase Peroxide); Group V- internal/external bleaching with 35% hydrogen peroxide (Opalescence Xtra) and Group VI- intracoronal bleaching with paste of sodium perborate mixed with 9% hydrogen peroxide. All the specimens were examined in SEM. The specimens of the Groups I, II and III were analised externally and the specimens of the Groups IV, V and VI were analised internal and externally. The SEM analysis showed some changes on the morphology of enamel, dentin and cementum of all bleached specimens, but the cementoenamel junction were the most affected part of the teeth. The bleaching agents used in this study caused alterations on the shapes of the cementoenamel junction increasing the exposition of dentin in this area and separating the enamel and the cementum. Kruskal-Wallis test was applied (H=6,947). The Groups I, III, V and VI showed similar results but the Groups II and IV showed identical behavior, more agressive than the others, but not statiscally significant.

Objective: The aim of this study was to evaluate the effect of Coca-Cola® on the surface roughness of conventional glass ionomer (Ketac™ Molar Easymix) versus resin modified glass ionomer cement (Ketac™ N100, Fuji II LC). Materials and methods: 10 samples for each glass ionomer cement were prepared using a brass mold of inner diameter 4mm x 2mm thickness. In group I: Ketac™ N100, group II: Fuji II LC and group III: Ketac molar Easymix. The surface roughness test was performed before and after to exposure the samples in Coca-Cola® through the rugosimeter. The samples of each group were immersed in 25ml of Coca-Cola® for 5 minutes, 1 time a day. After every inmersion, all the samples were placed in 25 ml of distilled water at 37 °C. This regimen was followed for 2 weeks. The ANOVA test was used to find and analyzed the results. Results: An initial average de 0.29μm ± 0.17μm, 0.19μm ± 0.07μm and 0.63μm ± 0.25μm; and a final average of 0.31μm ± 0.19μm, 0.23μm ± 0.08μm y 0.88μm ± 0.34μm for Ketac™ N100, Fuji II LC and Ketac™ Molar Easymix respectively. In addition, no statistically significant differences were found when comparing the initial and final surface roughness in each glass ionomer cement. However, when comparing the three ionomers statistically significant differences (p=0.000) were seen. Conclusion: The resin-modified glass ionomer cement Ketac™ N100 had the lowest Surface roughness. However Fuji II LC showed a similar behavior and can be a good alternative of treatment. / Objetivo: Evaluar la microrugosidad superficial del ionómero convencional (Ketac™ Molar Easymix) versus el ionómero modificado con resina (Ketac™ N100, Fuji II LC) sometidos a una gaseosa efervescente Coca-Cola®. Materiales y métodos: Se confeccionaron 30 muestras con una matriz cuadrada de 5mm x 5mm y 2mm de grosor de ionómeros, los cuales se dividieron 3 grupos según la marca. En el grupo I estuvieron las muestras del ionómero Ketac™ N100, en el grupo II las muestras del ionómero Fuji II LC y en el grupo III las muestras del ionómero Ketac™ Molar Easymix. Las muestras de ionómero estuvieron almacenados en recipientes con 25 ml de agua destilada, los cuales fueron tapados con papel aluminio y colocados en una estufa a 37°C. Se midió la rugosidad inicial a las 24 horas de confeccionadas las muestras. Posteriormente, se sumergieron en recipientes con 25 ml de Coca-Cola® por 5 minutos durante 2 semanas y luego se procedió a medir la microrugosidad final a través del rugosímetro. Resultados: Se encontró una media inicial de 0.29μm ± 0.17μm, 0.19μm ± 0.07μm y 0.63μm ± 0.25μm; y una media final de 0.31μm ± 0.19μm, 0.23μm ± 0.08μm y 0.88μm ± 0.34μm para el ionómero Ketac™ N100, Fuji II LC y Ketac™ Molar Easymix respectivamente, luego de ser sumergido en la Coca-Cola®. No se encontraron diferencias estadísticamente significativas al comparar la microrugosidad inicial y final en cada ionómero. Sin embargo, al comparar la 3 marcas de ionómero sí se observaron (p=0.000). Conclusión: El ionómero Ketac™ N100 fue el que presentó menor microrugosidad. Sin embargo, el ionómero Fuji II LC demostró tener un comportamiento parecido al Ketac™ N100 por lo que podría ser utilizado como alternativa de tratamiento. / Tesis

Objetivo: Evaluar la actividad antibacteriana de tres ionómeros de vidrio (CIVc) Ion Z®, Ketac Molar Easymix® y Fuji IX GP® sobre Streptococcus mutans (ATCC 35668) y Lactobacillus acidophilus (ATCC 4366). Materiales y métodos: El presente estudio fue experimental in vitro. Se realizó mediante el método de contacto directo y densidad óptica con una microplaca, donde se utilizó seis pocillos por cada ionómero de vidrio. Posteriormente, se adicionaron 150 ul de Streptococcus mutans y Lactobacillus acidophilus de manera independiente en agar BHI. Las muestras fueron incubadas a 37 °C en condiciones de anaerobiosis controlada. En el caso de la viabilidad bacteriana se utilizó el ensayo de MTT. Resultados: Se encontró que el efecto antibacterianode Ketac Molar Easymix® frente a la cepa de S. mutans tuvo 0.06 ± 0.01, mientras que Ion Z® 0.16 ± 0.03 ante la cepa de L. acidophilus, presentando diferencias estadísticamente significativas. Con respecto a la viabilidad bacteriana de S. mutans frente al Ketac Molar Easymix® obtuvo 0.92 ± 0.02, lo que corresponde a 29 % de bacterias viables, mientras que, L. acidophilus frente al Ion Z® tuvo 0.31± 16, que representa 19% de bacterias viables. Conclusiones: Los tres CIVs mostraron actividad antibacteriana, en el caso de Ketac Molar Easymix® demostró mayor efecto antibacteriano ante S. mutans, por otro lado, se evidenció que Ion Z® fue más eficaz frente L. acidophilus. / Objective: To evaluate the antibacterial activity of three glass ionomers cements (Ion Z®, Ketac Molar Easymix® and Fuji IX GP®) on Streptococcus mutans (ATCC 35668) and Lactobacillus acidophilus (ATCC 4366). Materials and methods: The present study was experimental in vitro. It was made out using the direct contact method and optical density with a microplate, using six wells for each glass ionomer cement. Later, 150 ul of Streptococcus mutans and Lactobacillus acidophilus were independently added on BHI agar. The samples were incubated at 37 °C under conditions of controlled anaerobiosis. In the case of bacterial viability, the MTT assay was used. Results: It was found that the antibacterial effect of Ketac Molar Easymix® against the strain of Streptococcus mutans had 0.06 ± 0.01, while in Z® it was 0.16 ± 0.03 before the strain of Lactobacillus acidophilus, presenting statistically significant differences. Regarding the bacterial viability of Streptococcus mutans against Ketac Molar Easymix® obtained 0.92 ± 0.02, what corresponds to 29% of viable bacteria, whereas Lactobacillus acidophilus against Ion Z® had 0.31± 16, which represents 19% of viable bacteria. Conclusions: The three glass ionomers cements showed antibacterial activity. Ketac Molar Easymix® demonstrated greater antibacterial effect against Streptococcus mutans; on the other hand, it was evidenced that Ion Z® was more effective against Lactobacillus acidophilus. / Tesis