Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, an Andean region in Peru. Methods: A total of 359 serum samples from patients with acute febrile illness were assessed for the presence of DENV via RT-PCR, ELISA NS1, IgM and IgG in Cajamarca, Peru from January 2017 to June 2017. The evaluation of the different diagnostic tests and their applicability was performed. Results: Dengue virus was detected in 24.7% of samples by RTPCR. Meanwhile, serological analysis detected 30.3% positive cases via ELISA NS1 antigen, 16.7% via ELISA IgG and 9.7% via ELISA IgM. Most of the cases corresponded to DENV-3 (77.5%). The use of RT-PCR performed better in primary infections (P<0.01), while detection of ELISA IgM performed better in secondary infections (P<0.01). The combination of NS1 and IgM performed better than the other assays in detecting primary (92.5%) and secondary infections (96.6%). The most frequent symptoms associated with fever were headaches, myalgias, and arthralgias across all groups. Conclusions: We report an important outbreak of dengue infection caused by DENV-3 in Cajamarca, Peru. Our findings encourage the use of NS1 antigen and IgM co-detection. These findings demonstrate an increasing expansion of DENV-3 in Peru and highlight the importance of molecular diagnosis and serotype characterization among the clinically defined dengue cases to strengthen the Peruvian epidemiological surveillance. / Ministry of Health and Welfare / Revisión por pares
| Identifer | oai:union.ndltd.org:PERUUPC/oai:repositorioacademico.upc.edu.pe:10757/658422 |
| Date | 01 October 2021 |
| Creators | Aguilar-Luis, Miguel, Carrillo-Ng, Hugo, Kym, Sungmin, Silva-Caso, Wilmer, Verne, Eduardo, Del Valle, Luis, Bazn-Mayra, Jorge, Zavaleta-Gavidia, Victor, Cornejo-Pacherres, Daniel, Tarazona-Castro, Yordi, Aquino-Ortega, Ronald, Cornejo-Tapia, Angela, Valle-Mendoza, Juana |
| Publisher | Wolters Kluwer Medknow Publications |
| Source Sets | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/article |
| Format | application/pdf |
| Source | Universidad Peruana de Ciencias Aplicadas (UPC), Repositorio Academico - UPC, Asian Pacific Journal of Tropical Medicine, 14, 10, 456, 462 |
| Rights | info:eu-repo/semantics/openAccess, Attribution-NonCommercial-ShareAlike 4.0 International, http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| Relation | https://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=10;spage=456;epage=462;aulast=Aguilar-Luis;type=0 |
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