Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. / This study:
1. Describes the preparation of unfermented and fermented rooibos methanol and aqueous
extracts.
2. Investigates the influence of unfermented and fermented rooibos methanol and aqueous
extracts on the binding of natural steroid substrates to ovine adrenal microsomal
cytochrome P450 enzymes, demonstrating that the binding of natural steroids is inhibited
in the presence of rooibos extracts.
3. Describes an assay demonstrating the inhibitory effect of rooibos extracts on the catalytic
activity of cytochrome 17α-hydroxylase (CYP17) and cytochrome 21-hydroxylase
(CYP21) in ovine adrenal microsomes.
4. Investigates the influence of unfermented and fermented rooibos methanol extracts on the
catalytic activity of individual cytochrome P450 enzymes – CYP17 and baboon CYP21,
that are expressed in COS1 cells.
5. Demonstrates that fractions of the unfermented rooibos methanol extract inhibits the
binding of natural steroid substrate to microsomal cytochrome P450 enzymes as well as
the catalytic activity of baboon CYP21 expressed in COS1 cells.
6. Investigates the inhibitory influence of individual rooibos flavonoids on the catalytic
activity of baboon CYP21 expressed in COS1 cells.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/2381 |
| Date | 03 1900 |
| Creators | Perold, Helene |
| Contributors | Swart, A. C., Swart, P., University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : University of Stellenbosch |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | University of Stellenbosch |
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