Thesisa (MSc (Genetics. Plant Biotechnology))--University of Stellenbosch, 2005. / The study had two main objectives:
1) to investigate specific enzyme activity profiles at various developmental
stages and to determine possible implications for sucrose metabolism,
2) to incorporate enzyme activity data of different internodes to obtain a
detailed model of every stage in the tissue maturation process.
The most significant findings of the regulation of sucrose accumulation in this
study are centred on three main point controls in sucrose metabolism pathway.
Firstly, the maturation of sugarcane internodes coincided with an increase of
SPS in most genotypes, and this underlines the key role of this enzyme in
sucrose accumulation. Secondly, SuSy activity (cleavage reaction) correlated
negatively with sucrose concentration and hence with tissue maturation process,
in most of the varieties. This finding indicates that SuSy could well be implicated
in sucrose metabolism. Thirdly, in vitro PFP activity was found to be negatively
correlated to sucrose content in sugarcane varieties differing in amount of
sucrose.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/2462 |
| Date | 12 1900 |
| Creators | Orendo-Smith, R. |
| Contributors | Botha, F. C., Groenewald, J-H. |
| Publisher | Stellenbosch : University of Stellenbosch |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
| Format | 518854 bytes, 139079 bytes, application/pdf, application/pdf |
| Rights | University of Stellenbosch |
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