A new column, Dionex AS4A, (polystyrenedivinylbenzene matrix) used for the separation of ribonucleotides and deoxyribonucleotides for the first time, and previously used for ion analysis was found superior to conventional silica columns because it separates ribonucleotides and deoxyribonucleotides. Resolution of dGTP was not possible with the Dionex column and CTP and GDP often co-eluted. Using conventional silica columns, monophosphates separated from diphosphates and diphosphates from triphosphates. Using the new Dionex column resolves all three simultaneously. The Dionex column resolved nucleotides with sharper peaks than silica columns, and the longer its retention time the better was the resolution. This Dionex column is stable, with 80 runs possible without cleaning while resolving ribonucleotides and deoxyribonucleotides to the picomole level.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500813 |
| Date | 12 1900 |
| Creators | Brock, Patricia C. (Patricia Charlene) |
| Contributors | O'Donovan, Gerard A., White, Olivia Masih, Smith, Don Wiley |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 64 leaves: ill., Text |
| Rights | Public, Brock, Patricia C. (Patricia Charlene), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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