In 2005 and 2010, high sea surface temperatures caused widespread coral bleaching on Jamaica’s north coast reefs. Three shallow (9m) reef sites were surveyed during each event to quantify the prevalence and intensity of coral bleaching. In October 2005, 29-57% of the colonies surveyed were bleached. By April 2006, 10% of the corals remained pale/partially bleached. Similarly, in October 2010, 23-51% of corals surveyed at the same sites were bleached. By April 2011, 12% of the colonies remained pale/partially bleached. Follow-up surveys revealed low coral mortality following both events, with an overall mean of 4% partial colony mortality across all species and sites observed in April 2006, and 2% in April 2011. Mixed effects models were used to quantify the relationship between colony size and (a) bleaching intensity, and (b) bleaching related mortality among coral species. The bleaching intensity model explained 51% of the variance in the bleaching response observed during the two events. Of this 51%, fixed effects accounted for ~26% of the variance, 17% of which was attributed to species-specific susceptibility to bleaching , 5% to colony size, <1% colony morphology and 4% to the difference in bleaching intensity between the two events. The random factor (site) accounted for the remaining ~25% of the variance. The mortality model explained 16% of the variance in post bleaching mortality with fixed effects, including colony size, morphology and species explaining ~11% of the variance, and the random effect (site) explaining 5%. On average, there was a twofold difference in bleaching intensity between the smallest and the largest size classes. Modelling the relationship between colony level characteristics and site-specific environmental factors on coral species’ susceptibility to thermal stress can shed light on community level responses to future disturbances.

Many consumer and medical products contain engineered nanomaterials (ENMs) due to their unique properties arising from their small size of <100 nm in at least one dimension. Although ENMs could greatly improve the quality of daily life, concerns for their health and environmental safety emerged in recent years because the same properties that make ENMs beneficial may also render them toxic. The small size allows ENMs’ entrance into the cell where they may attach to biological molecules and membranes, disrupting their function and/or leading to oxidative stress and/or damage. This thesis focused on silver nanoparticles (AgNPs). Several articles demonstrated that during washing AgNPs are released from the AgNP-impregnated fabrics and could pose a risk to aquatic species. Given that the toxicity mechanisms of AgNPs are yet to be clearly understood this thesis investigated the effects of AgNPs from ‘oxidative stress’ and ‘endocrine disruption’ points of view, using both in vivo and in vitro model fish systems. A 4 d exposure of zebrafish (Danio rerio) embryos to AgNPs increased mortality, delayed hatching, and increased oxidative stress. The silver ion (Ag+) was more effective in eliciting these effects at equivalent silver concentrations. Moreover, the Ag-chelator cysteine reduced the toxicity of both Ag-types. Despite these effects AgNPs or Ag+ did not affect the ability of zebrafish larvae or adults (raised to adulthood in Ag-free water) to increase cortisol levels, but there were differential effects on the expression of corticotropin-releasing factor (CRF)-related genes, suggesting that other physiological processes regulated by CRF may be impacted. Furthermore, a 48 h exposure of rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes to AgNPs or Ag+ increased oxidative stress, but Ag+ was more potent. Moreover, AgNPs elevated lipid peroxidation, while Ag+ increased DNA damage, suggesting different modes of action for the two Ag-types. Cysteine treatment reduced the toxicity of Ag+ and AgNPs, while buthionine sulfoximine, which inhibits glutathione synthesis, increased it, suggesting the importance of glutathione in silver toxicity. Finally, AgNPs increased glycogenolysis in trout hepatocytes independently of the beta-adrenoreceptor or the glucocorticoid receptor.

A large number of building inventory in Canada and elsewhere in the world consists of non-ductile reinforced concrete frames, with or without masonry infill panels. These structures suffer damage when seismic force demands are higher than their force capacities. Therefore, seismic retrofitting of such frame buildings for drift control remains to be a viable option for improved building performance. A retrofit methodology has been developed in the current research project, which involves diagonal bracing of frames with prestressing strands. An experimental research project has been conducted to assess the effectiveness of diagonal prestressing in non-ductile reinforced concrete frame buildings. The experimental program consists of two large-scale single-bay single-storey reinforced concrete frames, with a height of 3.0m and a span length of 3.5 m. The frames were designed and built to reflect the 1960’s practice in Canada, without the seismic requirements of current building codes, and hence are seismically deficient. They were retrofitted with diagonally placed prestressing strands, having two different areas of steel, prestressed to 40% of the strand capacity. One of the frames was retested after the failure of the strands, with a new set of strands without any prestressing, forming the third test. The results indicate that lateral bracing reinforced concrete frames with high-strength prestressing strands is an effective strategy for controlling lateral drift and hence potential damage in buildings during strong earthquakes. Prestressing of the strands increases initial stiffness, as compared to non-prestressed cables, and provide superior performance. The area of diagonally placed steel (including the number of strands) and the level of initial prestressing depend on the required level of upgrade in the building in terms of seismic force requirements. The design procedure recommended in this thesis may be employed for implementing the technology. The thesis presents the details of the experimental program, and the test results. It also provides analytical verification of the approach, with a step-by-step design procedure.

Much recent scholarship celebrates the early modern period for its development of broader public political engagement through printed media and coffeehouse culture. It is the argument of this study that the formation in England under Charles II of a public sphere may be shown to have followed a reassessment of political discourse that began at Westminster during the troubled reign of that king’s father, Charles I. The narrative of parliament’s growth in this era from an “event to an institution,” as one historian describes it, tells of more than opposition to the King on the battlefields of the English Civil War. Parliament-work in the early years of England’s revolutionary decade also set new expectations for rhetorical deliberation as a means of directing policy in the House of Commons. The ideals of discursive politics that were voiced in the Short Parliament (May 1640), and more fully put into practice in the opening session of the Long Parliament (November 1640), were soon also accepted by politically-minded authors and readers outside Westminster. Prose controversy published in print and political poetry that circulated in manuscript both demonstrate that the burgeoning culture of debate outside parliament could still issue “in a parliamentary way.” Such promotion of productive textual engagements eventually constituted a wider, notional assembly, whose participants – citizen readers – were as much a product of deliberate education and fashioning as they were of the “conjuring,” “interpellation,” or “summoning” that recent scholarly vocabulary suggests. Following the spirit of reform in the English parliament, and subsequently developing through the years of partisan political writing that followed, public opinion, like the Commons, established itself in this era as an institution in its own right. These public and private assemblies disseminated the unprecedented amount of parliamentary writing and record-keeping that distinguishes the period under review, and this rich archive provides the literary and historical context for this study.

Sensitive marine clay in Ottawa is a challenging soil for geotechnical engineers. This type of clay behaves differently than other soils in Canada or other parts of the world. They also have different engineering characteristic values in comparison to other clays. Cone penetration testing in sensitive marine clays is also different from that carried out in other soils. The misestimation of engineering characteristics from cone penetration testing can result. Temperature effects have been suspected as the reason for negative readings and erroneous estimations of engineering characteristics from cone penetration testing. Furthermore, the applicability of correlations between cone penetration test (CPT) results and engineering characteristics is ambiguous. Moreover, it is important that geotechnical engineers who need to work with these clays have background information on their engineering characteristics. This thesis provides comprehensive information on the engineering characteristics and behaviour of sensitive marine clays in Ottawa. This information will give key information to geotechnical engineers who are working with these clays on their behaviour. For the purpose of this research, fifteen sites in the Ottawa area are taken into consideration. These sites included alternative technical data from cone and standard penetration tests, undisturbed samples, field vanes, and shear wave velocity measurements. Laboratory testing carried out for these sites has resulted in acquiring engineering parameters of the marine clay, such as preconsolidation pressure, overconsolidation ratio, compression and recompression indexes, secondary compression index, coefficient of consolidation, hydraulic conductivity, clay fraction, porewater chemistry, specific gravity, plasticity, moisture content, unit weight, void ratio, and porosity. This thesis also discusses other characteristics of sensitive marine clays in Ottawa, such as their activity, sensitivity, structure, interface shear behaviour, and origin and sedimentation. Furthermore, for the purpose of increasing local experience with the use of cone and ball penetrometers in sensitive marine clays in Ottawa, three types of penetrometer tips are used in the Canadian Geotechnical Research Site No. 1 located in south-west Ottawa: 36 mm cone tip, and 40 mm and 113 mm ball tips. The differences in their response in sensitive marine clays will be discussed. The temperature effects on the penetrometer equipment are also studied. The differences in the effect of temperature on these tips are discussed. Correlations between the penetrometer results and engineering characteristics of Ottawa's clays are verified. The applicability of correlations between the testing results and engineering characteristics of sensitive marine clays in Ottawa is also presented in this thesis. Two correlations from the Canadian Foundation Engineering Manual are examined. One of these correlations is between the N60 values from standard penetration testing and undrained shear strength. The other correlation is between the shear wave velocity measurement and site class. Temperature corrections are suggested and discussed for penetrometer equipment according to laboratory calibrations. The significance of the effects due to radical temperature changes in Canada and Ottawa is discussed. Some of the main findings from this research are as follows. • The Canadian Foundation Engineering Manual presents a correlation between standard penetration tests (SPTs) and the undrained shear strength of soils. This relationship may not be applicable to sensitive marine clays in Ottawa. • Another correlation between the site class, shear wave velocity, and undrained shear strength is presented by this same manual which may not be applicable to sensitive marine clays in Ottawa. • The rotation rate for field vane testing as recommended by ASTM D2573 is slow for sensitive marine clays in Ottawa. • Correction factors applied to undrained shear strength from laboratory vane tests may not result in comparable values with the undrained shear strength obtained by using field vane tests. • Loading schemes in consolidation or oedometer testing may affect the quality of the targeted results. • Temperature corrections should be applied to penetrometer recordings to compensate for the drift in the results of these recordings due to temperature changes. • The secondary compression index to compression index ratio presented in the literature may not be the value obtained from this research.

The popularity of mother blogs is increasingly attracting sponsors looking to market their products to mother blog audiences. This combination of commercial and informational content calls on readers and writers to distinguish between informational content and commercial activity. This thesis examines how mother bloggers integrate sponsored content into their blogs and how both writers and readers interpret the credibility of these posts. The study takes place within a conceptual framework of source and message credibility. Using a qualitative and interpretive approach, semi-structured interviews were conducted with readers and writers. Although the results are not generalizable, they suggest determining the credibility of these posts is a social process, informed by participants’ existing knowledge and framed within the community of mother bloggers. The results add to our understanding of credibility perceptions when commercial and informational content comingle and have implications for other online communities that require ongoing information evaluation.

In Wireless Sensor and Robot Networks (WSRNs), static sensors report event information to one of the robots. In the k nearest neighbour query processing problem in WSRNs, the robot receives event report needs to find exact k nearest robots (KNN) to react to the event, among those connected to it. We are interested in localized solutions, which avoid message flooding to the whole network. Several existing methods restrict the search within a predetermined boundary. Some network density-based estimation algorithms were proposed but they either result in large message transmission or require the density information of the whole network in advance which is complex to implement and lacks robustness. Algorithms with tree structures lead to the excessive energy consumption and large latency caused by structural construction. Itinerary based approaches generate large latency or unsatisfactory accuracy. In this thesis, we propose a new method to estimate a search boundary, which is a circle centred at the query point. Two algorithms are presented to disseminate the message to robots of interest and aggregate their data (e.g. the distance to query point). Multiple Auction Aggregation (MAA) is an algorithm based on auction protocol, with multiple copies of query message being disseminated into the network to get the best bidding from each robot. Partial Depth First Search (PDFS) attempts to traverse all the robots of interest with a query message to gather the data by depth first search. This thesis also optimizes a traditional itinerary-based KNN query processing method called IKNN and compares this algorithm with our proposed MAA and PDFS algorithms. The experimental results followed indicate that the overall performance of MAA and PDFS outweighs IKNN in WSRNs.

With the introduction of antibiotics in animal feed becoming less popular, the agricultural industry has begun a shift towards the use of probiotics in animal feed. Since there is no current method to evaluate the risks of using genetically modified probiotics in animal feed. The goal of this project was to create a genetically modified model organism for risk assessment. The genetic marker for that was chosen was GFP that was to be expressed on the surface of the cell. The fluorescent properties allow for visualisation of the genetically modified bacteria and the surface expression would allow for the easy capture and recovery of the bacteria for culturing and cell counts. Genome wide screens were performed using the CW PRED algorithm to locate proteins with LPXTG motif for cell wall anchoring. 16 hypothetical proteins were detected and 6 were selected as candidates for possible surface display of GFP. Of these candidates, the novel L. casei protein LSEI_2320 was found to be expressed at the mRNA during early growth by RT PCR and at then protein level during stationary phase with western blot. This LPXTG protein was found at the surface of L. casei ATCC334 during stationary phase and late stationary phase with immunofluorescence microscopy. A genetically modified L. casei ATCC334 was constructed using the surface protein LSEI_2320 locus as a region for recombination with the pRV300 suicide plasmid. Genetic modification of the locus by the insertion of a GFP reporter region just before the predicted signal peptide site resulted in the abrogation of the expression of LSEI_2320 from the cell surface at the late stationary phase. It appears that this particular gene is not necessary to cell survival even though it is abundantly expressed on the cell surface and can be used as a location for genetic modification in L. casei ATCC334.

In this study, membranes were made from three polyvinylidene fluoride (PVDF) polymers individually and the blend systems of high (H) and low (L) molecular weight PVDF by phase inversion process. After investigating membrane casting solutions’ viscous and thermodynamic properties, the membranes so fabricated were characterized by scanning electron microscopy, gas permeation tests, porosity measurement, contact angle (CA) and liquid entry pressure of water (LEPw) measurement, and further subjected to vacuum membrane distillation (VMD) in a scenario that was applicable for cooling processes, where the feed water temperature was maintained at 27℃. It was found that PVDF solutions’ viscosities and thermodynamic instabilities were determined by the types of PVDF employed in single polymer systems and the mixing ratios of two PVDF polymers in blend systems. Thus the membrane properties and performances were influenced by the aforesaid factors as well. In single polymer systems, it was found that the membrane surface roughness and porosity increased with an increase in molecular weight. Among all the membranes casted in this study, the water vapor flux of VMD was found to be the highest at the intermediate range of H:L ratio, i.e., 4:6, at which the thickness of the sponge-like layer showed a minimum, the finger-like macro-voids formed a more orderly single-layer structure, and the LEPw showed a minimum. A conclusion can be made that blend systems of high molecular weight PVDF polymers and low molecular weight PVDF polymers could be used to optimize membrane performance in vacuum membrane distillation.

This thesis applies a novel method of change detection, the Landsat Image Stack Trend Analysis method to the monitoring of retrogressive thaw slumps in the Richardson Mountains, NWT. This method represents a significant improvement upon previous methods of thaw slump monitoring, which utilized air photos and high-resolution satellite imagery. This method applies Tasseled Cap brightness, wetness and greenness indices to Landsat TM/ETM images acquired between 1985 and 2011 and analyzes the temporal change of each pixel for the different indices values. This method is useful in retrogressive thaw slump monitoring in two ways. First, by creating a map showing the linear change over time from 1985 to 2011, retrogressive thaw slumps can be easily identified, as they are more dynamic than the surrounding tundra. In total, 251 thaw slumps were identified within an area of roughly 18 000km2. Second, thaw slump activity, from initiation, growth and stabilization can be studied by plotting the annual vegetation index pixel values of adjacent pixels in a thaw slump. This method allows for the efficient extraction of annual thaw slump headwall retreat rates, provided the availability of cloud-free imagery. The retreat rates of 16 slumps were extracted, which were found to have an average annual retreat rate of 11.8 m yr-1.