Thèse Sc. Genève.

Inaug.-Diss.--Würzburg. / eContent provider-neutral record in process. Description based on print version record.

Inaug.-Diss.--Würzburg. / eContent provider-neutral record in process. Description based on print version record.

Paris. Th. sc. (Universit́e). 1910-1911. No. 67.

Em janeiro de 2005, foram coletados 31 carrapatos adultos da espécie Amblyomma triste em uma propriedade rural da CESP, localizada no município de Paulicéia, Estado de São Paulo. Três carrapatos foram positivos para o teste de hemolinfa, demonstrando estruturas compatíveis com riquétsias no interior de hemócitos. Dois desses carrapatos foram submetidos à tentativa de isolamento de riquétsias em células Vero, através da técnica de Shell vial. Um isolado foi obtido com sucesso, estando já estabelecido em cultivo celular, com várias passagens e partidas congeladas. Do restante do carrapato utilizado para este isolamento, foi extraído o DNA e este foi submetido a PCR para um fragmento do gene ompA de Rickettsia spp. Foi realizada a caracterização molecular do isolado, através do sequenciamento genético de quatro genes de Rickettsia spp: gltA, htrA, ompA e ompB e os genes apresentaram 99,8 a 100% de identidade com as seqüências correspondentes de Rickettsia parkeri no GenBank. Todos os 31 carrapatos tiveram o DNA extraído, sendo processados pela PCR para um fragmento do gene gltA e para um fragmento do gene ompA. Três (9,7%) foram positivos a PCR para o gene gltA e os mesmos 3 carrapatos foram positivos para o ompA, sendo exatamente os 3 carrapatos previamente positivos ao teste de hemolinfa). O material amplificado destes 3 carrapatos para o fragmento de gene ompA foi processado para o sequenciamento automático de nucleotídeos resultando em 100% de identidade com a seqüência correspondente de Rickettsia parkeri no GenBank. Este trabalho relata pela primeira vez a bactéria R. parkeri no Brasil, o que foi confirmado pelo isolamento do agente em cultivo de células / In January 2005, 31 adult free-living ticks of the species Amblyomma triste were collected in the CESP rural farm located in the city of Paulicéia, state of São Paulo, Brazil. In the laboratory, 3 of these ticks were positive by the hemolymph test, showing structures compatible with Rickettsia within the hemocytes. Attempts to isolate Rickettsia were performed in two hemolymph-positive ticks by the Shell-vial technique. One isolate was successful obtained, being established in Vero cell culture, with several passages performed. DNA extracted from infected cells was submitted to PCR targeting fragments of four Rickettsia genes: gltA, htrA, ompA and ompB. DNA sequences obtained from PCR products of these four genes showed 99.8 to 100% of similarity with corresponding sequences of Rickettsia parkeri in the Genbank. DNA was extracted from all 31 ticks and processed by PCR targeting a fragment of the rickettsial gltA gene and a fragment of ompA gene. Three (9.7%) ticks were positive for both genes, being the same ticks previously positive by the hemolymph test. PCR products of these ticks were sequenced, being 100% identical to the corresponding sequence of Rickettsia parkeri in GenBanK. This study perfoms the first report of R. parkeri in Brazil, confirmed by the isolation of the agent in cell culture

Em janeiro de 2005, foram coletados 31 carrapatos adultos da espécie Amblyomma triste em uma propriedade rural da CESP, localizada no município de Paulicéia, Estado de São Paulo. Três carrapatos foram positivos para o teste de hemolinfa, demonstrando estruturas compatíveis com riquétsias no interior de hemócitos. Dois desses carrapatos foram submetidos à tentativa de isolamento de riquétsias em células Vero, através da técnica de Shell vial. Um isolado foi obtido com sucesso, estando já estabelecido em cultivo celular, com várias passagens e partidas congeladas. Do restante do carrapato utilizado para este isolamento, foi extraído o DNA e este foi submetido a PCR para um fragmento do gene ompA de Rickettsia spp. Foi realizada a caracterização molecular do isolado, através do sequenciamento genético de quatro genes de Rickettsia spp: gltA, htrA, ompA e ompB e os genes apresentaram 99,8 a 100% de identidade com as seqüências correspondentes de Rickettsia parkeri no GenBank. Todos os 31 carrapatos tiveram o DNA extraído, sendo processados pela PCR para um fragmento do gene gltA e para um fragmento do gene ompA. Três (9,7%) foram positivos a PCR para o gene gltA e os mesmos 3 carrapatos foram positivos para o ompA, sendo exatamente os 3 carrapatos previamente positivos ao teste de hemolinfa). O material amplificado destes 3 carrapatos para o fragmento de gene ompA foi processado para o sequenciamento automático de nucleotídeos resultando em 100% de identidade com a seqüência correspondente de Rickettsia parkeri no GenBank. Este trabalho relata pela primeira vez a bactéria R. parkeri no Brasil, o que foi confirmado pelo isolamento do agente em cultivo de células / In January 2005, 31 adult free-living ticks of the species Amblyomma triste were collected in the CESP rural farm located in the city of Paulicéia, state of São Paulo, Brazil. In the laboratory, 3 of these ticks were positive by the hemolymph test, showing structures compatible with Rickettsia within the hemocytes. Attempts to isolate Rickettsia were performed in two hemolymph-positive ticks by the Shell-vial technique. One isolate was successful obtained, being established in Vero cell culture, with several passages performed. DNA extracted from infected cells was submitted to PCR targeting fragments of four Rickettsia genes: gltA, htrA, ompA and ompB. DNA sequences obtained from PCR products of these four genes showed 99.8 to 100% of similarity with corresponding sequences of Rickettsia parkeri in the Genbank. DNA was extracted from all 31 ticks and processed by PCR targeting a fragment of the rickettsial gltA gene and a fragment of ompA gene. Three (9.7%) ticks were positive for both genes, being the same ticks previously positive by the hemolymph test. PCR products of these ticks were sequenced, being 100% identical to the corresponding sequence of Rickettsia parkeri in GenBanK. This study perfoms the first report of R. parkeri in Brazil, confirmed by the isolation of the agent in cell culture

Thèse de doctorat--Droit--Paris, 1931.

Blackwood's Edinburgh Magazine was arguably the most important and widely published literary magazine of the nineteenth century. Its readership extended from Britain to America, shaping literary tastes across the Anglophone literary marketplace. BEM wrote two reviews of Nathaniel Hawthorne's fiction during the author's most prolific years. The first was published in 1847 and contained a lengthy reflection of the state of American literature that prefaced its review of Mosses from an Old ManseM. In 1855, BEM reviewed Hawthorne's novels. The language of these reviews encouraged BEM's transatlantic readership to interpret Hawthorne in a very particular light: a dark, intense, and deeply psychological Hawthorne. In other words, BEM promoted a version of Hawthorne that would ultimately stick and become the standard Hawthorne adopted by twentieth-century historians of the "American Renaissance." I argue that BEM's reviews reveal a relationship with American literature predisposed to appreciate a dark, symbolic, gothic literature, and that Hawthorne, like Irving before him, succeeded in becoming one of the greatest writers of mid-nineteenth-century American literature because he was able to appeal to and please a transatlantic, and particularly a British, audience. By transcending geographic boundaries, at least in BEM's reviews, Hawthorne was ironically identified as an iconic "American" writer.

Blackwoods Edinburgh Magazine was arguably the most important and widely published literary magazine of the nineteenth century. Its readership extended from Britain to America, shaping literary tastes across the Anglophone literary marketplace. BEM wrote two reviews of Nathaniel Hawthornes fiction during the authors most prolific years. The first was published in 1847 and contained a lengthy reflection of the state of American literature that prefaced its review of Mosses from an Old Manse. In 1855, BEM reviewed Hawthornes novels. The language of these reviews encouraged BEMs transatlantic readership to interpret Hawthorne in a very particular light: a dark, intense, and deeply psychological Hawthorne. In other words, BEM promoted a version of Hawthorne that would ultimately stick and become the standard Hawthorne adopted by twentieth-century historians of the œAmerican Renaissance. I argue that BEMs reviews reveal a relationship with American literature predisposed to appreciate a dark, symbolic, gothic literature, and that Hawthorne, like Irving before him, succeeded in becoming one of the greatest writers of mid-nineteenth-century American literature because he was able to appeal to and please a transatlantic, and particularly a British, audience. By transcending geographic boundaries, at least in BEMs reviews, Hawthorne was ironically identified as an iconic œAmerican writer.

For the last forty years, Jane Eyre criticism has understandably focused on Bertha Mason Rochester as a marginalized, abused, and silenced mixed-race woman. Although Jane's childhood friend Helen Burns is a very different and much less controversial character, she and Bertha suffer similar deaths from the culpable neglect of their guardians. Both women serve as the impetus of a bystanders dilemma: the perennial question of whether a person is obligated to protect another's life or dignity at the risk of his or her own. Because contemporary law imposed no duty to rescue upon bystanders, this paper uses the commentary of Victorian legal theorist John Austin to create a standard against which to judge the ethical merit of the choices made by bystanders throughout the novel. Maria Temple, superintendent of Lowood, is a bystander to the fatal abuse heaped upon her students; she has the power to expose the schools brutal conditions, but chooses to remain silent so that she can keep her job and her limited power. Her choice, while practical, makes her complicit in Helen's death. When Jane becomes bystander to Bertha's dangerously negligent captivity, she chooses to flee Thornfield rather than intervene. Though many critics have decried her selfishness, Jane makes a practical and ethical choice because she has so little chance of helping Bertha and so much to lose in the attempt. Just as Miss Temple is able to protect Jane because of her self-serving decisions, Jane in turn is able to protect Adele. Yet all these successes are predicated upon earlier neglect of persons unable to protect themselves, as Helen and Bertha remind us. There is no comfortable solution to the bystanders dilemma.