Acute stretching increases postural stability in nonbalance trained individuals. J Strength Cond Res 26(11): 3095-3100, 2012-Studies into the relationship between acute stretching and maintenance of postural balance have been inconclusive. It was hypothesized that familiarization with the task and subsequent learning might be involved in the conflicting results. Therefore, this study was to designed determine if a regimen of static stretching exercises after a familiarization period would improve a person's ability to maintain a stabilometer in a neutral position and whether stretching had the same effect on individuals with extensive involvement with balancing tasks. Forty-Two college students (21 male, 21 female) and 10 surfers (all male) performed tests on a stabilometer on 2 separate days after 3 days of familiarization. Testing followed either 30 minutes of quiet sitting (nonstretched) or 30 minutes of stretching activities (stretched). Stretching exercises consisted of various assisted and unassisted static stretches of the muscles around the hip, knee, and ankle joints. Improved flexibility after the stretching exercises was demonstrated by significant (p , 0.05) 6.5 6 2.7 cm (mean 6 SD) increase in the sit and reach. Balance time for the students improved significantly by 11.4% (2.0-second increase), but the surfers had no significant change. Thus, stretching improved maintenance of balance perhaps by helping the subjects to eliminate the gross muscle contractions that caused large stabilometer displacements and to replace them with fine muscle contractions that caused little or no stabilometer displacements. However, it appears that experience doing balance tasks supplants any stretching benefit.

We detail for the first time the uniquely altered fibrin polymerization of homophenotypic Aα R16H dysfibrinogen. By polymerase chain reaction amplification and DNA sequencing, our new proposita's genotype consisted of a G>A transition encoding for Aα R16H, and an 11 kb Aα gene deletion. High-performance liquid chromatography disclosed fibrinopeptide A release approximately six times slower than its fibrinopeptide B. Turbidimetric analyses revealed unimpaired fibrin repolymerization, and abnormal thrombin-induced polymerization (1-7 μmol/l fibrinogen, > 96% coagulable), consisting of a prolonged lag time, slow rate, and abnormal clot turbidity maxima, all varying with thrombin concentration. For example, at 0.2-3 U/ml, the resulting turbidity maxima ranged from lower to higher than normal control values. By scanning electron microscopy, clots formed by 0.3 and 3 thrombin U/ml displayed mean fibril diameters 42 and 254% of the respective control values (n = 400). Virtually no such differences from control values were demonstrable, however, when clots formed in the presence of high ionic strength (μ = 0.30) or of monoclonal antiβ(15-42)IgG. The latter also prolonged the thrombin clotting time approximately three-fold. Additionally, thrombin-induced clots displayed decreased elastic moduli, with G′ values of clots induced by 0.3, 0.7 and 3 thrombin U/ml corresponding to 11, 34, and 45% of control values. The results are consistent with increased des-BB fibrin monomer generation preceding and during polymerization. This limited the inherent gelation delay, decreased the clot stiffness, and enabled a progressively coarser, rather than finer, network induced by increasing thrombin concentrations. We hypothesize that during normal polymerization these constitutive des-BB fibrin monomer properties attenuate their des-AA fibrin counterparts.

Magnetic resonance elastography (MRE) Is capable of non-invasively quantifying the mechanical properties of skeletal muscles in vivo. This information can be clinically useful to understand the effects of pathologies on the mechanical properties of muscle and to quantify the effects of treatment. Advances in inversion algorithms quantify muscle anisotropy in two-dimensional (2D) and three-dimensional (3D) imaging. Databases of the shear stiffness of skeletal muscle have been presented in the relaxed and contracted states in the upper extremity (biceps brachii, flexor digitorum profundus, and upper trapezius), distal leg muscles (tibialis anterior, medial gastrocnemius, lateral gastrocnemius, and trapezius), and proximal leg muscles (vastus lateralis, vastus medialis, and sartorius). MRE measurements have successfully validated a mathematical model of skeletal muscle behavior in the biceps brachii, correlated to electromyographic data in the distal leg muscles and quantified the effects of pathologies on the distal and proximal leg muscles. Future research efforts should be directed toward improving one-dimensional (1D) and 3D MRE data acquisition and image processing, tracking the effects of treatment on pathologic muscle and correlating the shear stiffness with clinical measurements.

Compacted soils are frequently used as subgrade for pavements as well as commercial and residential buildings. The stiffness and shear strength properties of compacted soils, which are collectively denoted as Ω in this thesis, fluctuate with moisture content changes that result from the influence of environmental factors such as the evaporation and infiltration. For example, mechanistic pavement design methods require the information of resilient modulus (MR), which is the soil stiffness behavior under cyclic traffic loading, and its variation with respect to the soil moisture content determined from laboratory tests or estimation methods. Significant advances have been made during the last five decades to understand and model the variation of the Ω with respect to soil moisture content and soil suction (s) based on the principles of mechanics of unsaturated soils. There are a variety of models presently available in the literature relating the Ω to the s using different approaches. There are however uncertainties extending these models for predicting Ω - s relationships when they are used for a larger soil suction range. In addition, the good performance of these models are only valid for certain soil types for which they were developed and calibrated. Studies presented in this thesis are directed towards developing a unified methodology for modelling the relationship between the Ω and the s using limited while easy-to-obtain information. However, more emphasis has been focused on the MR - s relationships of pavement subgrade soils considering the need for the application of the mechanistic pavement design methods in Canada. The following studies have been conducted: (i) State-of-the-art review on existing equations in the literature for the MR - s relationships is summarized. A comparison study is followed to discuss the strengths and limitations of these equations; (ii) A unified methodology for modelling the Ω - s relationships is proposed. Experimental data on 25 different soils are used to verify the proposed unified methodology. The investigations are applied on small strain shear modulus, elastic modulus, and peak and critical shear strength. Good predictions are achieved for all of the investigated soils; (iii) Performance of the proposed methodology is examined for the MR - s relationships using experimental data of 11 subgrade soils. Reasonably good predictions are achieved for all of the subgrade soils; (iv) Extensive experimental investigations are conducted on the MR - s relationships for several subgrade soils collected from various regions in Canada. Experimental results suggest non-linear variation in the MR with respect to s, moisture content and the external stress. The measured results are modelled using the proposed methodology with adequate success; (v) Additional experimental investigations are performed to determine the variation of the elastic modulus (E) and unconfined compression strength (qu) with the s and the gravimetric moisture content (w) for several Canadian subgrade soils. An approach, which is developed extending the proposed unified methodology, is used to normalize the measured MR - w, E - w and qu - w relationships. It is shown that the normalized MR - w, E - w and qu - w relationships exhibit remarkable similarity and can be well described using the proposed approach. Such similarity in the normalized Ω - moisture content relationships are also corroborated using the experimental data on several other soils reported in the literature. The proposed unified methodology alleviates the need for the determination of the Ω - s relationships which requires elaborate testing equipment that needs the supervision of trained personnel and is also time-consuming and expensive. In addition, experimental programs in this thesis provide detailed experimental data on the MR, E, qu, and soil-water characteristic curves of Canadian subgrade soils. These data will be helpful for the better understanding of the hydro-mechanical behavior of the Canadian subgrade soils and for the implementation of the mechanistic pavement design method in Canada. The simple tools presented in this thesis are promising and encouraging for implementing the mechanics of unsaturated soils into conventional geotechnical engineering practice.

This paper presents results of an experimental investigation to study the structural performance and deformability of a concrete bridge deck reinforced with corrosion resistant reinforcing (CRR) bars, i.e., bars that exhibit improved corrosion resistance when embedded in concrete as compared to traditional black steel. Flexural tests of one-way slabs were conducted to simulate negative transverse flexure over a bridge girder as assumed in the commonly employed strip design method. The bar types studied were Grade 60 (uncoated), epoxy-coated reinforcing (ECR, Grade 60), Enduramet 32 stainless steel, 2304 stainless steel, MMFX2, and glass fiber reinforced polymer (GFRP). The experimental program was designed to evaluate how a one-to-one replacement of the Grade 60 with CRR, a reduction of concrete top clear cover, and a reduction in bar quantities in the bridge deck top mat influences flexural performance at service and ultimate limit states. Moment-curvature predictions from the computer-based sectional analysis program Response 2000 were consistent with the tested results, demonstrating its viability for use with high strength and non-metallic bar without a defined yield plateau.    Deformability of the concrete slab-strip specimens was defined with ultimate-to-service level ratios of midspan deflection and curvature. The MMFX2 and Enduramet 32 one-to-one replacement specimens had deformability consistent with the Grade 60 controls, demonstrating that bridge deck slabs employing high strength reinforcement without a defined yield plateau can still provide sufficient ductility at an ultimate limit state. A reduction in bar quantity and cover provided acceptable levels of ductility for the 2304 specimens and MMFX2 reinforced slabs. / Master of Science

The knee is the most complex joint in the human body. It consists of a system of muscle, bone, and ligaments that endures repetitive loading during daily and athletic activities. When this loading is excessive, damage  to the knee occurs leading to a decreased quality of life.The medial collateral ligament (MCL) is one of the 4 major ligaments known to be commonly injured in the knee. The risk of injury to the knee joint increases with the elderly and individuals who experience chronic dehydration. For this reason, the focus of this study is to compare different mechanical quantities that can be used to analyze damage to the MCL. In this study, a novel mechanical testing protocol is used to progressively induce damage in dehydrated rat MCLs by performing tensile tests. This involves stretching the ligaments along their longitudinal axes to consecutive and increasing displacements starting at a 0.4 mm displacement and in increments of 0.2 mm until complete failure occurs. The load and change in length that the ligament experiences are measured at each displacement. Three different methods were evaluated to determine subfailure and damage propagation in rat MCLs: changes in tangent stiffness and chord stiffness, and changes in the load value at the 0.4 mm displacement for each load-displacement curve. The findings of this study indicate that the tangent stiffness and load at the 0.4 mm displacement provide information of the early onset of damage propagation. The decrease in chord stiffness of the ligament does not indicate damage progression in the ligament, but rather is the sign of the imminent failure of the MCL.This study provides insightful data into understanding the subfailure damage in the MCL. / Master of Science

Chip and scrub seal treatments are common pavement preservation practices that use asphalt emulsions. Their performance has been studied for several years, yet many questions remain. The primary thesis objective was to study near surface behaviors of flexible pavements that are candidates for seal treatments. This study investigated the ability of the bending beam rheometer (BBR) to detect pavement surface changes due to the application of asphalt emulsion. Estimated stiffness and m-value data was recorded for three asphalt concrete mixtures using mixture beams approximately 120 mm long by 12 mm wide by 7.7 mm thick sawn from the surface of asphalt specimens. One mixture was plant mixed and laboratory compacted, while the other two were field-aged mixtures. This study gauges effect of treating specimens with emulsions commonly used in Mississippi in conditioned and unconditioned states.

Breast cancer metastasis of a variety of vital organs is a major cause of breast cancer mortality. Autophagy has a crucial role in the metastatic breast cancer progression. As a critical mechanotransducer in the Hippo signalling pathway, YAP regulates cell proliferation and promotes autophagy. Previous publications also demonstrated extracellular matrix could regulate the nucleo-cytoplasmic transport of YAP. However, how YAP signalling connects to the interplay of autophagy and mechanotransduction in breast cancer metastasis remains entirely unknown. Through rapamycin-induced autophagy on the metastatic triple negative breast cancer (TNBC) cells, we observed upregulated YAP transcriptional activity and YAP nuclear localization in TNBC. Thus, we reported that YAP nuclear localization regulates autophagy to promote TNBC metastasis. Culturing TNBC cells on PDMS plates with various matrix stiffness demonstrated that stiff matrix promoted the migration of metastatic breast cancer cells in a YAP-dependent mechanism. Therefore, we proposed that YAP mechanotransduction promotes the migration of metastatic breast cancer cells. Then, we advance in these directions by reporting autophagy-mediated YAP nuclear localization is regulated by the response to stiff matrix when TNBC cells were cultured on different matrix stiffness upon autophagy. In conclusion, we suggest autophagy and mechanotransduction mediates YAP nuclear localization together. These findings expand the unknown gap in the convergence of YAP mechanotransduction and autophagy in metastatic breast cancer. They suggest that metastatic breast cancer cells have the potential to exhibit different YAP signalling when they colonize on a secondary location with a distinct matrix stiffness from primary location. Our study further helped to understand YAP biology and the mechanism of breast cancer metastasis that will shed light on future YAP-targeting therapeutics for metastatic breast cancer. / Thesis / Master of Applied Science (MASc)

Composites have high strength-to-weight ratios, which is particularly desired for applications with weight restrictions. Common composite materials such as carbon fiber reinforced plastic (CF) and fiber glass reinforced plastic (FG) were used in this research. While composite materials possess high stiffness and strength properties, the stiffness of composite laminates may be maximized by changing the geometry. By adding corrugations, the flexural stiffness is increased in one direction compared to the stiffness of a flat part with the same amount of material. Thus, stiffness increases without a change in weight. The primary goal of this research was to investigate the stiffness characteristics of corrugated composite laminates under tensile and flexural load. The chosen corrugation geometry for investigation was a trapezoid. To observe the effects of corrugations, both flat and corrugated coupons were tested experimentally with the same procedures. Stiffness was calculated experimentally, analytically, and numerically in both directions. In this study, the longitudinal direction was defined as perpendicular to the corrugations and transverse direction was defined as the direction along the corrugations. The effects on stiffnesses of corrugated and flat composites were measured by comparing changes to the stiffness ratios in tension and bending. The stiffness ratio is the ratio of longitudinal stiffness to transverse stiffness. The secondary aim of this research was to compare the corrugation effects on FG weave and cross-ply CF. This was interesting to observe the difference in corrugation effects on different composite materials. The FG laminates were manufactured from four plies of pre-impregnated Cytec MXB 7701/7781. The CF laminate consisted of five plies of pre-impregnated unidirectional Tencate TC250/M46J. The layup orientation of the CF laminate had alternating 0◦ and 90◦ plies, where the 0 ◦ plies were in the transverse direction. Plies were directly laid on a flat plate and aluminum mold for flat and corrugated specimens, respectively. All flat and corrugated composites were cured in an autoclave under respective recommended cure cycles for each material. The tension and three-point bend tests were conducted on an Instron 8800 where the load was applied at a rate of 0.05 inches per minute. The tensile ultimate load was the same between corrugated and flat specimens in the longitudinal direction. Meanwhile, the tensile ultimate load was greatly reduced for corrugated specimens in the transverse direction when compared to the flat specimens. Thus, corrugations had a larger impact in the transverse direction under tensile load for both materials. By corrugating the composite layups, the ratio of stiffness in the longitudinal to extensional direction increases. For FG test coupons, the extensional stiffness ratio was increased from 1.0 to 49.3 due to corrugations. The flexural stiffness ratio was increased from 0.3 to 187.1 in corrugated FG coupons. For CF test coupons, the extensional stiffness ratio increased from 0.7 to 61.3. The flexural stiffness ratio of CF test coupons increased from 0.3 to 81.4. Corrugations had a greater effect on the cross-ply CF for both extensional and flexural stiffnesses.

The dynamics of wrist rotations are dominated by joint stiffness, which the neuromuscular system must account and compensate for when controlling wrist movements. Because wrist stiffness is anisotropic, movements in some directions require less torque than movements in others, creating opportunities to follow "paths of least resistance." Forearm pronation-supination (PS) can combine with wrist flexion-extension (FE) and radial-ulnar deviation (RUD) to allow the wrist to rotate in directions of least stiffness. Evaluating this hypothesis, and understanding the control of combined wrist and forearm rotations in general, requires a knowledge of the stiffness (the dominant mechanical impedance) encountered during combined wrist and forearm rotations. While wrist and forearm stiffness have been measured in isolation, there are no measurements of coupled wrist and the forearm stiffness. This study characterizes the passive stiffness of the wrist and forearm in combinations of FE, RUD, and PS. Using a wrist and forearm robot, we measured coupled wrist and forearm stiffness for 15° movements from neutral position in 10 young, healthy subjects. We found the stiffness in PS to be significantly smaller than the stiffness in RUD, but similar in magnitude to the stiffness in FE, indicating that the torque required to overcome stiffness in combinations of PS and FE is significantly smaller than the torque required to overcome stiffness in combinations of FE and RUD (assuming equal displacements). The coupled stiffness measured here will enable future studies to determine optimal paths and to compare these optimal paths to observed movements involving wrist and forearm rotations.