As últimas décadas trouxeram grandes avanços tecnológicos às ciências forenses. Um dos marcos dessa evolução foram às pesquisas e os resultados obtidos com a aplicação da Biologia Molecular, como ferramenta de identificação humana a partir da década de 80. Desde então, novos estudos vêm sendo realizados nesta área. Vestígios encontrados em locais de crime são elementos que irão orientar na busca pela elucidação dos fatos. Existem dois tipos de vestígios: os macrovestígios, facilmente identificados e os microvestígios que demandam análises técnicas mais específicas. Dentre os microvestígios, tem-se a impressão digital, que se tornou uma possível fonte de extração de DNA, com um grande potencial de recuperação do material genético. Este trabalho objetivou analisar amostras coletadas em microvestígios de impressões digitais em vários objetos escolhidos como superfície de deposição sendo elas, vidro, metal, plástico, madeira e parede de alvenaria, demonstrando que é possível estabelecer uma ligação entre as amostras de DNA e as impressões digitais encontradas. As amostras foram coletadas de impressões latentes intactas e em esfregaço e impressões digitais intactas e em esfregaço com pó. Os resultados demonstraram a viabilidade de utilização deste tipo de amostra, tendo em vista a recuperação de DNA e o êxito da genotipagem. Os resultados obtidos nas diferentes matrizes analisadas evidenciaram maior êxito na superfície de metal, onde foi possível obter perfil genético íntegro em todas as amostras coletadas e analisadas. Com relação à matriz vidro, nas amostras “intacta latente” e “esfregaço latente” foi possível recuperar perfil genético com mais de 17 locos amplificados. Já nas amostras “intactas e esfregaço com pó”, mesmo com a confirmação da presença de DNA, as quantidades recuperadas foram insuficientes para gerar o eletroferograma. Na matriz madeira, assim como na matriz plástico, foi constatada a presença de DNA, mas em baixa concentração para gerar o eletroferograma. E, por último, as amostras coletadas da matriz parede de alvenaria “intacta latente” e “intacta com pó”, apresentaram respectivamente amplificação de 17 e 19 locos dos 24 presentes no kit. Estudos e experimentos já tornaram esta metodologia viável no Laboratório de Genética Molecular Forense da Polícia Científica do Estado do Paraná, com resultados positivos em diversos casos, identificando suspeitos e contribuindo com a Rede Integrada de Banco de Perfis Genéticos (RIBPG). Os resultados demonstraram a eficiência e a possibilidade de se obter um perfil genético quando se trabalha com este tipo de amostra, tornando esta mais uma ferramenta pericial. / The last decades have brought great technological advances to the Forensic Sciences. The Molecular Biology has been used as a tool for human identification since the 80´s, and it has bought fantastic results from this application, being a landmark in the evolution of Forensic Science. Since this decade, new studies have been carried out in this area. Traces found in crime scenes are elements that can guide the search for the elucidation of the facts. There are two types of traces: macro-traces, that are easily identified and micro-traces that requires more specific technical analysis. One of the traces is the digital fingerprint, that is a possible source of DNA extraction, with great potential for recovery of the genetic material. This research has the purpose to analyze samples collected from fingerprints on various objects chosen as deposition surface, such as glass, metal, plastic, wood and masonry wall. This research shows that it is possible to establish a connection between DNA samples and fingerprints. Samples have been collected from intact and intact smears and fingerprints intact and smeared with powder. The results showed the feasibility of using this type of sample, based on the DNA recovery and the success of the genotyping. The results obtained in the different matrices analyzed showed greater results in the metal surface, where it was possible to obtain a complete genetic profile in all the samples Collected and analyzed. In the glass matrix, either the samples "latent intact" or in "latent smear" it was possible to recover genetic profile with more than 17 amplified loci. In the "intact and powder smear" samples, even with confirmation of the presence of DNA, the quantities recovered were insufficient to generate the electropherogram. In the wood matrix, such as in the plastic matrix, the presence of DNA was observed, but at low concentration to generate the electropherogram. Finally, the samples collected from the "latent intact" and "intact with powder" masonry wall samples, respectively, showed amplification of 17 and 19 loci of the 24 present in the kit. Some Studies and experiments have been done in the Forensic Molecular Genetics Laboratory of Scientific Police in Paraná with positive results in many cases, identifying suspects and contributing to the Integrated Network of Gene Prolifiling Banks (RIBPG). These studies have made this methodology feasible. The results show the efficiency and the possibility of obtaining a genetic profile from this type of sample, making this one more important pericial tool.

As últimas décadas trouxeram grandes avanços tecnológicos às ciências forenses. Um dos marcos dessa evolução foram às pesquisas e os resultados obtidos com a aplicação da Biologia Molecular, como ferramenta de identificação humana a partir da década de 80. Desde então, novos estudos vêm sendo realizados nesta área. Vestígios encontrados em locais de crime são elementos que irão orientar na busca pela elucidação dos fatos. Existem dois tipos de vestígios: os macrovestígios, facilmente identificados e os microvestígios que demandam análises técnicas mais específicas. Dentre os microvestígios, tem-se a impressão digital, que se tornou uma possível fonte de extração de DNA, com um grande potencial de recuperação do material genético. Este trabalho objetivou analisar amostras coletadas em microvestígios de impressões digitais em vários objetos escolhidos como superfície de deposição sendo elas, vidro, metal, plástico, madeira e parede de alvenaria, demonstrando que é possível estabelecer uma ligação entre as amostras de DNA e as impressões digitais encontradas. As amostras foram coletadas de impressões latentes intactas e em esfregaço e impressões digitais intactas e em esfregaço com pó. Os resultados demonstraram a viabilidade de utilização deste tipo de amostra, tendo em vista a recuperação de DNA e o êxito da genotipagem. Os resultados obtidos nas diferentes matrizes analisadas evidenciaram maior êxito na superfície de metal, onde foi possível obter perfil genético íntegro em todas as amostras coletadas e analisadas. Com relação à matriz vidro, nas amostras “intacta latente” e “esfregaço latente” foi possível recuperar perfil genético com mais de 17 locos amplificados. Já nas amostras “intactas e esfregaço com pó”, mesmo com a confirmação da presença de DNA, as quantidades recuperadas foram insuficientes para gerar o eletroferograma. Na matriz madeira, assim como na matriz plástico, foi constatada a presença de DNA, mas em baixa concentração para gerar o eletroferograma. E, por último, as amostras coletadas da matriz parede de alvenaria “intacta latente” e “intacta com pó”, apresentaram respectivamente amplificação de 17 e 19 locos dos 24 presentes no kit. Estudos e experimentos já tornaram esta metodologia viável no Laboratório de Genética Molecular Forense da Polícia Científica do Estado do Paraná, com resultados positivos em diversos casos, identificando suspeitos e contribuindo com a Rede Integrada de Banco de Perfis Genéticos (RIBPG). Os resultados demonstraram a eficiência e a possibilidade de se obter um perfil genético quando se trabalha com este tipo de amostra, tornando esta mais uma ferramenta pericial. / The last decades have brought great technological advances to the Forensic Sciences. The Molecular Biology has been used as a tool for human identification since the 80´s, and it has bought fantastic results from this application, being a landmark in the evolution of Forensic Science. Since this decade, new studies have been carried out in this area. Traces found in crime scenes are elements that can guide the search for the elucidation of the facts. There are two types of traces: macro-traces, that are easily identified and micro-traces that requires more specific technical analysis. One of the traces is the digital fingerprint, that is a possible source of DNA extraction, with great potential for recovery of the genetic material. This research has the purpose to analyze samples collected from fingerprints on various objects chosen as deposition surface, such as glass, metal, plastic, wood and masonry wall. This research shows that it is possible to establish a connection between DNA samples and fingerprints. Samples have been collected from intact and intact smears and fingerprints intact and smeared with powder. The results showed the feasibility of using this type of sample, based on the DNA recovery and the success of the genotyping. The results obtained in the different matrices analyzed showed greater results in the metal surface, where it was possible to obtain a complete genetic profile in all the samples Collected and analyzed. In the glass matrix, either the samples "latent intact" or in "latent smear" it was possible to recover genetic profile with more than 17 amplified loci. In the "intact and powder smear" samples, even with confirmation of the presence of DNA, the quantities recovered were insufficient to generate the electropherogram. In the wood matrix, such as in the plastic matrix, the presence of DNA was observed, but at low concentration to generate the electropherogram. Finally, the samples collected from the "latent intact" and "intact with powder" masonry wall samples, respectively, showed amplification of 17 and 19 loci of the 24 present in the kit. Some Studies and experiments have been done in the Forensic Molecular Genetics Laboratory of Scientific Police in Paraná with positive results in many cases, identifying suspects and contributing to the Integrated Network of Gene Prolifiling Banks (RIBPG). These studies have made this methodology feasible. The results show the efficiency and the possibility of obtaining a genetic profile from this type of sample, making this one more important pericial tool.

Concretes reinforced by, using distributed steel reinforcements (fibres) are known as fibre-concrete. In case of disturbances or accidents of concrete structures reinforced with wires, it is necessary to carefully examine the actual implementation of dispersed reinforcement. Fibre concretes belong to modern building materials whose possible applications have not been fully utilized so far. Have been mainly used for floor structures loaded with factory halls and warehouses. Recently, thanks to well-known physical and mechanical properties of fibre-concrete, there were numerous attempts of designers, and namely investors, to utilize this kind of materials for support structures either. Favorable properties of wire-concrete can be utilized if there is a necessity to increase the resistance of concrete to stresses exceeding its strength, cyclic stress or impact stress. Daily practice shows to prove that the applications of fibre-concrete in such structures lead to the economic success. Necessary condition for successful application of steel fiber reinforced concrete in constructions however consists in its uniform dispersion, a homogeneous distribution of the wires throughout the volume of the structure. In case of inappropriate processing and deposition of the mixture during the manufacturing process fiber-concrete structures, the fibers are often unevenly distributed. Wires itself represent unfavourably shaped mixture components and they are extremely deteriorating its workability. A grouping of wires may be encountered as well, which reduces the overall homogeneity and the quality of steel fiber-concrete structures. If the homogeneity of fibre-concrete is not kept, the material possess different properties in various parts of the structure (for example, tensile strength), which can lead to defects in the structure (generation and development of cracks). The relevant lower reliability of the structure which is caused by unequal distribution of fibres (wires) in concrete volume can lead to damage of the property as well as the safety and the human lives can be jeopardized. Hence it is necessary to secure the effective control of the fibre-concrete homogeneity in ready support fibre-concrete structures. Contemporary homogeneity control is still ongoing on fresh blends, but if the fibre-concrete hardened and is a part of the construction, no known reliable methods are currently in available to test the homogeneity of the fibre-concrete on the structure without its destruction. The methods developed to control the concentration of wires in wire-concrete structures are based mostly on magnetic or electromagnetic properties of wires. The thesis deals with the development of the magnetic method in situ using permanent magnets for monitoring the distribution of fibers in hardened steel fiber-concrete structures. The test principle is based on measurements of the changes in magnetic field strength of permanent magnets which are induced by a change in wire distribution in steel fibre-concrete structure. Test is characterized as a so called local- failure- test using a small diameter core drill. In this sense it is a semi-destructive method.

The topic of my master´s thesis was to create an architectural study of the Fakulty of Fine Arts in Brno - city part Veveří. The site lies near the Faculty of Law of Masaryk University and it´s bordered by streets Šumavská, Veveří, Bulínova and Akademická. The project ties together the architectural study from subject TG02 witch solved the second part of the programme "Academic Square" and it was a construction of the fakulty of Forensic Engineering and the background for both faculties. So the solution is a compex architectural study which sorts the urban and also the architectural aspects of both faculties and their background. The main idea of the urban concept is to také up the surrounding bulit-up-area which is typical for this part of Brno. The philosophy of the architectural solution comes from the concept of the traditional British universities. So the solution are two objects of the faculties with plane shape od the letter U which clutches the volume of the common background between each other.

The dissertation deals with problems in the valuation of parts and accessories of land with an emphasis on the difference between landscaping and engineering structure. Despite the existence of defined components and accessories immovable in the Civil Code in practice often appear doubts about whether in a particular case is still part of the real property, or whether it is already a matter of self. The work addresses this issue in detail only for roads, drainages and retaining walls. There were studied various laws and the earlier Court decisions. The Court judicature often solves problems of ownership determination. Can be seen as an example: Determining ownership of the rock, that is formed by hardening the land surface rocks and planting permanent crops. Whether the owner of the land owner or the person who built it. Likewise, there are disputes to determine ownership of utility connections, ownership structures, which are kept below the surface of the land. Based on the new Civil Code no. 89/2012 Coll. there has been extensive changes in the field of things and their allocation. There is a new concept existing, instead of the current concept of property. There is existing a new concept called Real property. A very important change is that the engineering structures are parts of the land, but based on the § 509 utilities, especially water, sewer or power lines or other, are not parts of the land. It is believed that parts of the utilities are also buildings and technical equipment that is connected to them operationally. Also important is the § 3055 para. (1): Engineering structure which is connected to the ground with a solid foundation, which is under the existing legislation part of the land on which it is established, and the effective date of this Act, owned by a person different from the owner of the land, and the bottom the entry into force of this Act does not become part of the land and immovable property. The same applies to the building, which is co-owned, if one of the joint owners and the owner of the land or if they are just some of venturers building co-owners of the land. The author presents the general specifics valuation external works and civil engineering in selected cases and the valuation of construction law. Work includes a chronological overview of valuation rules.

The dissertation focuses on the problems associated with determining the amount of wear (finding the remaining technical values) that are being addressed. It deals with an overview of the different methods used. The basic premise of the thesis is a comprehensive overview of the calculation procedure and the methods used. This area of wear does not except the price regulation precisely defined procedure, nor are they addressed the circumstances in which that would be the method to be used. Various possible methods or inaccuracies may result in questioning the expert opinions as at trial. In conclusion of this dissertation is shown how the calculation procedure should wear for what purpose should be used and it is also proposed several alternatives.

The dissertation focuses on the problem of determining the timeliness of the normal market rent an apartment at the market price of the apartment. The basic premise is obtain a database of prices of flats and apartments to compare these parameters the most accurate way. This area has been addressed several times, but only in some cities and not fully explored. The conclusions of the dissertation indicate what the relationship is normal and usual rental price of the apartment, then further comparison of the development of this relationship before the economic crisis, ie the price levels of 2008 and the present. Based on these data, it is also possible to determine the level of capitalization in each regional cities with regard to the average wage in each regional cities.

This PhD thesis analyzes the current, by experts in minimum benchmark solved condition of valuation of damaged, unlicensed and unauthorized buildings, it defines the problematic status of the individual administrative areas where the expert report is the decisive evidence and it recommends suitable method of valuation of these buildings, so as to minimize the risk of bringing faulty expert opinion. The paper not only explains the basic concepts identified in the relevant legislation, but also systematically analyzes the possible ways of determining the usual price of mentioned buildings. Derived ways of valuation methods are validated on examples and evaluated. On the basis of this verification there is a recommended procedure to determine the usual price of these types of buildings, ie. the expert standard.

This thesis is focused on possibilities of financial valuation of flexible pavements surface layers conditions, in connection with whole life cycle of the pavement and certain possibilities of their maintenance and repairs, in cases of defects and failures from forensic engineering point of view. There were carried out analysis of the topics connected with judging of flexible pavements surface layers defects and failures related to forensic investigation in the Czech Republic, as well as abroad. In this thesis there were created pricing models of the certain construction works which are related to repairs and maintenance of flexible pavements. Within the possibilities of the author, there were compared market prices of the particular construction works in selected regions with the prices according to generally used pricing systems in the Czech Republic. The goal of this thesis is to create unified methodology for amount of damage financial valuation in cases of defects and failures of flexible road pavements surface layers for forensic engineering practice in the Czech Republic.

This dissertation is focused on the identification and evaluation of specific factors affecting the price of agricultural land. It should be mentioned that the market for agricultural land, especially arable land and permanent grasslands, is not fully developed in the Czech Republic and this area is not fully explored. The thesis describes the factors on the basis of which the investor decides when buying these plots. The data needed for this work have been obtained from professional literature, real estate servers, two polls, actual sales, etc. In the conclusion of this thesis all findings are summarized and evaluated. It includes the development of prices in the years 2014 – 2016 based on sales actually realized in the Olomouc Region, Moravian – Silesian Region, South Moravian Region and Vysočina Region. The outcome of the thesis is a proposal of an expert standard. It is necessary to realize that, above all, agricultural land is a specific commodity, as it is unreproducible, indivisible and its offer is limited.