No description available.

DGCR8 (DiGeorge critical region 8) protein constitutes part of the Microprocessor complex together with Drosha, and is involved in the nuclear phase of microRNA (miRNA) biogenesis. DGCR8 recognises the hairpin RNA substrates of precursor miRNAs through two double-stranded RNA (dsRNA) binding motifs and acts as a molecular anchor to direct Drosha cleavage at the base of the pri-miRNA hairpin. Recent characterisation of the RNA targets of the Microprocessor by HITSCLIP of DGCR8 protein revealed that this complex also binds and regulates the stability of several types of transcripts, including mRNAs, lncRNAs and retrotransposons. Of particular interest is the binding of DGCR8 to mature small nucleolar RNA (snoRNA) transcripts, since the stability of these transcripts is dependent on DGCR8, but independent of Drosha. This raises the interesting possibility that there could be alternative DGCR8 complex/es using different nucleases to process a variety of cellular RNAs. We performed mass spectrometry experiments and revealed that DGCR8 copurifies with subunits of the nucleolar exosome, which contains the exonuclease RRP6. We demonstrated DGCR8 and the exosome form a nucleolar complex, which degrade the mature snoRNAs tested within this study. Interestingly, we also show that DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC), and absence of DGCR8 creates a concomitant telomere phenotype. In order to identify the RNA targets of the DGCR8/Exosome complex on a global scale we performed iCLIP of endogenous and overexpressed RRP6 (wild-type and a catalytically inactive form). Thus, intersection of CLIP datasets from DGCR8 and RRP6 identified common substrates; accordingly snoRNAs were the most represented. In addition, we identified the cellular RNA targets of the RRP6 associated human exosome. The use of a catalytically inactive form of RRP6 stabilised important in vivo interactions that are highly dynamic and transient and also highlighted the role of RRP6-mediated trimming of 3’flanks of immature non-coding RNAs. We will present a global view of the RNA-binding capacity of the RRP6-associated exosome. In sum, we identified a novel function for DGCR8, acting as an adaptor to recruit the exosome to structured RNAs and induce their degradation. Moreover, we have identified DGCR8-depenedent substrates of the exosome and have demonstrated the requirement of RRP6 for 3’ processing of ncRNAs.

Vid tillfällen då det inte lämpar sig att stå vid en videokamera kan det lösas med ett fjärrstyrt kamerafäste. Exempel på tillfällen är på grund av platsbrist på en konsert, en högtidsceremoni där någon måste stå konstigt till för att få bra bild, ute i naturen där djur ska filmas utan att bli ivägskrämda eller vid personalbrist, en tight budget och måste styra flera kameror samtidigt. De system som finns tillgängliga på amatörmarknaden idag har antingen annan funktionalitet och passar sig inte för ovanstående problem eller har väldigt begränsad räckvidd och går inte att sammankoppla med en mobilapplikation. Detta projekt går ut på att ta fram en kostnadseffektiv och skräddarsydd lösning för ovanstående problem genom att utveckla ett fjärrstyrt kamerafäste i semiproffssegmentet där det via en handkontroll och vid senare skede med en mobilapplikation styr kamerans lutning och rotation även kallad tiltning och panorering. Skillnaderna mellan denna och befintliga lösningar är att med hjälp av Bluetooth-tekniken kan styra både med handkontroll och senare även med en mobilapplikation, anledningen till att det skall kunna styra med båda är att det inte alltid är tillåtet att använda sig av mobiltelefoner i alla miljöer där man vill filma och därför får ett bredare användningsområde för kamerafästet. / At times when it is not suited to stand by a video camera can be resolved with a remote controlled camera mount. Examples of occasions is due to lack of space at a concert, a solemn ceremony in which someone must stand awkwardly  to to get good image, out in nature where animals shall be filmed without being frightened away or when you have staff shortages, a tight budget and need to control multiple cameras simultaneously. The systems that are available in the amateur market today have different functionality and is not fitting for the above problem or have very limited range and cannot connect to a mobile application. This project aims to develop a cost-effective and customized solution for the above problem by developing a remote-controlled camera mount in semi-professional segment where you through a controller and at a later stage with a mobile application controls the camera angle and rotation also called tilt and pan. The differences between this and existing solutions are that by using Bluetooth technology can control both the controller and later on also with a mobile application, the reason why it must be able to control both is that it is not always allowed to use cell phones in all environments where you want to film and therefore have a wider range of applications for camera mounting.

No description available.

Address trace compression represents that the address data, which are generated from the instruction fetch stage of the microprocessor, can be retrieved for later observation and analysis. This real time trace compression hardware is the primary component of real-time trace system. In this paper, we present how to design and implement this real-time address trace compressor. Address trace compressor is allowed to perform accurate, successive trace collection in an unlimited length and can be used in various embedded microprocessors without influencing the operation of the microprocessors. Also, it has abundant reconfigurable parameters that can be used to develop a cost-effective trace system. The experiment results show that this compressor can reach a higher compression ratio of 1:100. Hence, by utilizing this real-time compression technique, the trace depths of new trace system can be 20 times more than these existing in-circuit emulators.

In this paper, we present how to optimize our ARM7TDMI instruction set compatible microprocessor. The ARM7TDMI is a 32-bit microprocessor developed by ARM Ldt. It used in embedded application such as mobile phones, pager and PDAs. The ARM7 family owes its success to the combination of low power, low cost and high performance. However, as applications become more complex and integrate more and more functionality, the processor is required to provide more and more performance. We tune the hardware simply, no use complex hardware, to complete performance optimization. We use synthesis tool to synthesize our RTL design and analyze timing to fund the critical path of our microprocessor. We will describe how to optimize the critical path to increase performance.

In this paper, we will explore architecture variations of ARM7 microprocessors. The ARM7TDMI is a 32-bit microprocessor developed by ARM Ltd. It used in embedded application such as mobile phones, pager and PDAs. However, as portable products grow in complexity more processing power is required. This paper focuses on the implementation of the architecture variations of ARM7 microprocessors. The evolutions from our RTL design to architecture variations are described and the trade offs between high performance and low gate count discussed.

The Cross Compiler is an excellent and valuable program development tool. It provides to the user a low level compiled language that allows character (byte), integer (8086 word) and pointer (8086 one word address) manipulation. It also allows recursion, has modern flow and a rich set of operators.The Communication Program which include file transfer utility allows the student to download or upload their C program to a PC. It allows use of the Modem. The file transferring can be done using XON/XOFF or XMODEM. It also supports INS 8250 UART chip, plus 16450 high speed device found in hardware such as IBM AT Serial/Parallel Adapter. / Department of Computer Science

No description available.

Thesis (M.S.)--Brigham Young University. Dept. of Computer Science, 2004. / Includes bibliographical references (p. 49-52).