Orientador: Rosemary Adriana Chiérici Marcantonio / Resumo: A Terapia Fotodinâmica Antimicrobiana (Antimicrobial Photodynamic Therapy - aPDT), tem sido utilizada como um tratamento complementar na doença periodontal (DP). Ela combina um fotossensibilizador (FS) com uma fonte de luz que induz a produção de espécies reativas de oxigênio que elimina células microbianas. O objetivo deste trabalho foi avaliar, in vivo, os efeitos da aPDT (com o FS ftalocianina de zinco tetracarboxi-N-metilglucamina - 10μg/mL e luz LED vermelho - 655mn, 0,45W de potência), coadjuvante a Raspagem e Alisamento Radicular (RAR) e como monoterapia, além de verificar as respostas e alterações teciduais da DP induzida em ratos, pelas avaliações: microtomográfica, histométrica, estereométrica e histológica. Ligaduras foram inseridas nos sulcos dos segundos molares superiores para indução da DP. No Estudo 1, as ligaduras permaneceram por 15 dias e foram removidas para aplicar os tratamentos e no Estudo 2, as ligaduras foram colocadas por 7 dias e continuaram em posição por todo o experimento. 40 animais foram utilizados no Estudo 1 e distribuídos em 4 grupos: DP (Somente indução da doença, sem tratamento); RAR (Indução e tratamento básico periodontal); aPDT (Indução e aplicação da aPDT - FS ftalocianina de zinco tetracarboxi-N-metilglucamina e luz LED vermelho); RAR+aPDT (Indução, tratamento básico periodontal e aplicação da aPDT). 42 animais foram utilizados no Estudo 2 e divididos também em 4 grupos: FS (Tratamento somente com... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Antimicrobial Photodynamic Therapy (PDT) is a minimally invasive method consisting in the application of a photosensitive dye, which is subsequently stimulated by a light source and reacts with oxygen, producing reactive species. The aim of this study was to evaluate in vivo, the aPDT effects (with the PS zinc tetracarboxy-N-metylglucamine phthalocyanine 10μg/mL, and red LED light with 655nm), as adjuvant treatment to Scaling Root and Planing (SRP) and as monotherapy and verify the responses and tissue changes after aPDT application in PD-induced rats by microtomographic, histometric, stereometric and histological evaluations. Ligatures were placed around the second maxillary molars for PD induction. In Study 1, the ligatures were placed for 15 days and then they were removed. On the following day the treatments were performed. In Study 2, the ligatures were placed for 7 days and remained in position throughout the periods. Forty animals were used in Study 1 and they were divided into 4 groups: PD (disease induction only, without treatment); SRP (induction and basic periodontal treatment); PDT (Induction and application of photodynamic therapy); SRP+PDT (induction, application of photodynamic therapy and basic periodontal treatment). Forty-two animals were used in Study 2 and they divided into 4 groups: PS (Treatment with zinc tetracarboxy-N-metylglucamine phthalocyanine only); Light (Treatment with red LED light irradiation only); aPDT (Treatment with photodynamic therapy - ... (Complete abstract click electronic access below) / Doutor

Bc. Marek Budiš Comparison of X-ray microtomography possibilities of application in metrology with conventional approaches Master thesis, Institute of production machines, systems and robotics, BUT FME Brno This master thesis is focused on the analysis of metrological possibilities of using computer X-ray microtomography methods in the industry. The work contains a description of the methods, equipment and measurement. Listed are the main advantages of using X-ray tomography in metrology in comparison with coordinate measuring machine. The work deals with the measurement accuracy of microtomography machine GE V|tome|x L 240, analyzing sources of instrument uncertainty and its quantification. The work was also devised a practical table for the calculation of uncertainty of measurement. The practical part was also measuring on real samples of the industry.

Doctor of Philosophy / Department of Grain Science and Industry / Hulya Dogan / Jon Faubion / Bread quality and final crumb grain are reflective of the ability for wheat flour dough to retain and stabilize gas cells during the baking process. The visco-elastic properties of dough allow for the incorporation of air cells and expansion during fermentation and baking. The gluten-starch matrix provides the backbone support. However, following the end of proofing and during the beginning of baking, the structure weakens due to over-extension and expansion and the matrix begins to separate and eventually break down. Native wheat lipids, which are found in small quantities in wheat flour, provide a secondary support for gas cell stabilization because of their amphiphilic characteristics and ability to move to the interface and form condensed monolayers. The objectives of this research were to evaluate the influence of native wheat lipids on the rheological properties of dough and the microstructure of bread. Native wheat lipids were extracted from straight-grade flour and separated into total, free, bound, nonpolar, glycolipids, and phospholipids using solid-phase extraction (SPE) with polar and nonpolar solvents. Defatted flour was reconstituted using each lipid fraction at a range of levels between 0.2% and 2.8%. Dough and bread were made following AACC Method 10-10.03. Rheological testing of the dough and evaluation of the microstructure of the bread was conducted using small and large deformation testing, C-Cell imaging, and x-ray microtomography analysis to determine changes in visco-elastic properties and gas cell structure and distribution. Rheological assessment through small amplitude oscillatory measurements demonstrated that nonpolar, phospholipids, and glycolipid fractions had a greater interaction with both proteins and starch in the matrix, creating weaker dough. Nonpolar, phospholipids, and glycolipids, varied in their ability to stabilize gas cells as determined by strain hardening index. C-Cell imaging and x-ray microtomograpy testing found that treatments containing higher concentrations of polar lipids (glycolipids and phospholipids) had a greater effect on overall loaf volume, cell size, and distribution. This illustrates that level and type of native wheat lipids influence the visco-elastic properties of dough and gas cell size, distribution, cell wall thickness, and cell stability in bread.

Master of Science / Department of Grain Science and Industry / Hulya Dogan / Viscoelastic wheat flour doughs are renowned for their ability to produce high quality aerated bread products. Dough exhibits extremely complex rheological properties which makes it capable of occluding and retaining gas cells. The ability of these bubbles to resist failure and remain stable throughout the proofing and baking process is critical to final bread structure and volume. Understanding these factors is important when creating the distinct structural and textural characteristics that consumers desire in baked products. In this study, a method was established for using X-ray microtomography (XMT) to study the microstructure of proving dough as well as bread made from three very different wheat flours. Doughs were prepared according to AACC Method 10-10B optimized straight-dough bread-making method. Sections from unproofed (0 min), underproofed (20 min) and optimally proofed (40 min) doughs were carefully cut and frozen at –80°C. Baked loaves were also prepared following standard test bake procedures. Small specimens were cut from two locations of both the proofed and baked loaves prior to microstructural analysis. A total of 96 dough and bread samples were scanned using a high resolution desktop X-ray micro-CT system Skyscan1072 (Skyscan, Belgium) consisting of an X-ray tube, an X-ray detector and a CCD-camera. X-ray images were obtained from 137 rotation views through 180° of rotation. Hundreds of reconstructed cross sectional images were analyzed using CTAn (v.1.7) software. 3-D analysis of the bubbles indicated that average dough void fractions increased dramatically over proof time from 30.9% for the unproofed dough (0 min) to 62.0% and 74.5 % for the underproofed (20 min) and optimally proofed (40 min) doughs respectively. Oven spring caused further expansion in the baked loaves which increased average void fraction to 84.3%. Gas cell size distributions were largely skewed to the right and shifted in that same direction as processing time increased. Differences in gas cell size seen among flour varieties were largely due to variations in the size of the largest cells caused by coalescence.

The fundamental issue of determining acoustic properties of porous media from their local geometry is examined in this PhD dissertation thesis, thanks to a sample of open-cell aluminum foam analyzed by axial computed microtomography. Various geometric properties are measured to characterize the experimental sample at the cell size level. This is done in order to reconstruct a porous medium by means of idealized three- and two- dimensional unit-cells.The frequency dependant thermal and velocity fields governing the propagation and dissipation of acoustic waves through rigid porous media are computed by Brownian motion simulation and the finite element method, respectively. Macroscopic behavior is derived by spatial averaging of the local fields. Our results are compared to experimental data obtained from impedance tube measurements. Firstly, this approach leads to the identification of the macroscopic parameters involved in Pride and Lafarge semiphenomenological models. Secondly, it yields a direct access to thermal and viscous dynamic permeabilities. However, the bi-dimensional model underestimates the static viscous permeability as well as the viscous characteristic length; what thus require a three-dimensional implementation.

This is an investigation of new vertebrate fossil material from the Late Devonian locality of Ivanovka, Uryup River, Siberia. This bone bed material, circa 375 million years in age, represents a unique opportunity to fill a gap in our understanding of Late Devonian diversity, biogeography, and vertebrate evolution: Siberia, at the time, was an independent continent, and yet its fauna remains virtually unknown in comparison with the other paleocontinents, Euramerica and Gondwana. Using synchrotron microtomographic scanning, a non-destructive technique that has never, to our knowledge, been applied to bone bed material, we obtained 3D image stacks that were then modelled to yield triangle meshes representing the bones in three dimensions. These meshes could then be identified, described, and interpreted. Many of the discovered bones belong to the poorly known genus Megistolepis Obruchev 1955, potentially allowing a radical increase in knowledge regarding this taxon. Other material includes lungfish and possible fragments of limbed tetrapods, though the evidence of the latter is scarce. A discussion of the advantages and disadvantages of synchrotron microtomography for the study of bone bed material concludes the paper.

O objetivo do presente estudo foi avaliar, por meio de microtomografia computadorizada (micro-CT), o volume de remanescente de diferentes materiais obturadores (AH Plus e BC Sealer), após o uso do instrumento R50 e de técnicas complementares (inserto ultrassônico e instrumento XP-endo Finisher R) em canais radiculares ovais. Vinte e oito raízes distais de molares inferiores, com canal único e oval, foram selecionadas por micro-CT, preparados com instrumento R40 (40.06), e obturados com os seguintes cimentos: AH Plus (n = 14) e BC Sealer (n = 14), e submetidos à ciclagem térmica. Em seguida foi realizada a remoção inicial com R50 e os dentes foram escaneados no micro-CT para determinação do volume remanescente inicial de material obturador, e posterior complementação com inserto ultrassônico (n = 7) ou XP-endo Finisher R (n = 7). Os espécimes foram novamente escaneados, e a partir do volume inicial de material obturador (volinicial) e volume de material obturador remanescente (volrem) em mm3, obteve-se a % de volume de material remanescente após as técnicas de complementação (%volrem=volrem×100/volinicial). Para a análise qualitativa, as imagens de MEV e os modelos tridimensionais obtidos por micro-CT, foram avaliados em relação à presença/ausência e localização do remanescente de material obturador nos canais radiculares. Os dados de %volrem e volume remanescente de material obturador após o uso do instrumento R50 (%volR50) foram comparados pelo teste T, e os dados de %volrem foram submetidos ao teste ANOVA, seguido de teste de Tukey (nível de probabilidade de 95%). Considerando o canal como um todo, as técnicas de complementação promoveram redução significativa do volume remanescente de material obturador, sendo que menores valores foram observados quando utilizado o inserto ultrassônico (8,93 ± 6,12) em relação ao XP-endo Finisher R (16,26 ± 14,71) (P = 0,043). Quanto aos cimentos, pode-se observar que o BC Sealer apresentou maiores quantidades de remanescente (18,87 ± 13,42) quando comparado ao cimento AH Plus (6,33 ± 4,25) (P < 0,001). Em relação à avaliação por terços radiculares, observou-se que quando utilizado o instrumento XP-endo Finisher R, não houve diferença do volume de material remanescente entre os terços, independente do cimento obturador utilizado (P > 0,05). Entretanto, ao se utilizar inserto ultrassônico e cimento AH Plus, maiores valores de volume de material remanescente foram observados no terço apical (23,08 ± 18,1) (P < 0,001), e quando utilizado o BC Sealer, no terço médio (15,53 ± 6,0) (P = 0,022). Conclui-se que as técnicas de complementação aumentaram a remoção de material obturador, entretanto, nenhuma das técnicas removeu por completo o material obturador do canal radicular, principalmente quando o dente foi obturado com o cimento BC Sealer / The aim of the present study was to evaluate, by micro-computed tomography (micro-CT), the remaining volume of different filling materials (AH Plus and BC Sealer) after using the R50 instrument and supplementary techniques (ultrasonic insert and XP-endo Finisher R instrument) in oval-shaped root canals. Twenty-eight distal roots of mandibular molars with single and oval-shaped canals, verified by micro-CT, were prepared with R40 instrument (40.06), and filled with the following sealers: AH Plus (n = 14) and BC Sealer (n = 14), and subjected to thermocycling. Then, the initial removal was performed with R50 and the samples were scanned in micro-CT to determine the initial remaining filling material volume, and subjected to the supplementary techniques with ultrasonic tip (n = 7) or XP-endo Finisher R (n = 7). The specimens were again scanned, and the initial volume of filling material (volinitial) and the final volume of filling material after the supplementary techniques (volfinal), obtained in mm3, were used to calculate the volume % of remaining filling material (%volrem=volrem×100/volinitial). For the qualitative analysis, the SEM images and the three-dimensional models obtained by micro-CT were evaluated regarding the presence/absence and location of the remaining filling material in the root canals after retreatment. The data of %volrem and volume of the remaining filling material after R50 (%volR50) were compared by the T test, and the %volrem data were submitted to the ANOVA and Tukey tests (95% probability level). Considering the entire root canal, the supplementary techniques promoted significant reduction of the volume of the remaining filling material, and lower values were observed for the ultrasonic tip (8.93 ± 6.12) when compared to XP-endo Finisher R (16.26 ± 14.71) (P = 0.043). Regarding the different sealers, BC Sealer presented higher amounts of remaining material (18.87 ± 13.42) when compared to AH Plus (6.33 ± 4.25) (P <0.001). The evaluation by root thirds showed that when the XP-endo Finisher R instrument was used, there was no difference in the volume of remaining material between the thirds, regardless the sealer used (P > 0.05). However, when using ultrasonic tip and AH Plus, higher values were observed in the apical third (23.08 ± 18.1) (P <0.001), and when the BC Sealer was used, in the middle third 15.53 ± 6.0) (P = 0.022). It was concluded that supplementary techniques increased the removal, however, none of the techniques completely removed the root canal filling material, especially when the tooth was filled with BC Sealer. with R40 instrument (40.06), and filled with the following sealers: AH Plus (n = 14) and BC Sealer (n = 14), and subjected to thermocycling. Then, the initial removal was performed with R50 and the samples were scanned in micro-CT to determine the initial remaining filling material volume, and subjected to the supplementary techniques with ultrasonic tip (n = 7) or XP-endo Finisher R (n = 7). The specimens were again scanned, and the initial volume of filling material (volinitial) and the final volume of filling material after the supplementary techniques (volfinal), obtained in mm3, were used to calculate the volume % of remaining filling material (%volrem=volrem×100/volinitial). For the qualitative analysis, the SEM images and the three-dimensional models obtained by micro-CT were evaluated regarding the presence/absence and location of the remaining filling material in the root canals after retreatment. The data of %volrem and volume of the remaining filling material after R50 (%volR50) were compared by the T test, and the %volrem data were submitted to the ANOVA and Tukey tests (95% probability level). Considering the entire root canal, the supplementary techniques promoted significant reduction of the volume of the remaining filling material, and lower values were observed for the ultrasonic tip (8.93 ± 6.12) when compared to XP-endo Finisher R (16.26 ± 14.71) (P = 0.043). Regarding the different sealers, BC Sealer presented higher amounts of remaining material (18.87 ± 13.42) when compared to AH Plus (6.33 ± 4.25) (P <0.001). The evaluation by root thirds showed that when the XP-endo Finisher R instrument was used, there was no difference in the volume of remaining material between the thirds, regardless the sealer used (P > 0.05). However, when using ultrasonic tip and AH Plus, higher values were observed in the apical third (23.08 ± 18.1) (P <0.001), and when the BC Sealer was used, in the middle third 15.53 ± 6.0) (P = 0.022). It was concluded that supplementary techniques increased the removal, however, none of the techniques completely removed the root canal filling material, especially when the tooth was filled with BC Sealer

O objetivo do presente estudo foi analisar, por meio de microtomografia computadorizada, as alterações bidimensionais (área e perímetro), tridimensionais (volume, área de superfície e SMI) e o transporte do canal nas raízes mesiais de molares inferiores com curvatura severa, após o preparo biomecânico realizado por quatro sistemas de NiTi variando o tipo de cinemática e conceito de preparo, a geometria e design dos instrumentos e o tipo de tratamento da liga. Dessa forma, foram avaliados o sistema rotatório de instrumentos múltiplos Protaper Next (PTN); o sistema reciprocante de instrumento único Reciproc (RC); o sistema reciprocante de instrumento único, Reciproc Blue (RCB) e o sistema rotatório de instrumentos múltiplos TRUShape (TRS). Foram selecionados 32 molares inferiores com ângulo de curvatura da raiz mesial de 25-50º e raio 4 mm, e escaneados em microtomógrafo 1176 (SkyScan, Kontich, Bélgica), com resolução isotrópica de 18 m. Após isso, as imagens foram reconstruídas e analisadas com obtenção dos parâmetros iniciais (pré-instrumentação) bidimensionais de área e perímetro, e tridimensionais de volume, área de superfície e SMI, além do transporte do canal por meio da obtenção dos valores do centroide. Os espécimes foram então distribuídos em grupos experimentais de acordo com o sistema de instrumentação utilizado (n=8), e após a instrumentação, foi realizada nova análise microtromográfica seguindo os padrões iniciais descritos. Os dados pré e pós instrumentação foram tabulados e então realizada a análise estatística por meio de ANOVA e pós teste Tukey, com nível de significância de 5%. Os resultados demonstraram que, independente do sistema de instrumentação, houve alterações dos parâmetros bidimensionais e tridimensionais, como também transporte do canal pós-instrumentação. Não foram observadas diferenças estatisticamente significantes (p>0,05) entre os sistemas RC e RCB, bem como entre os sistemas PTN e TRS em nenhum dos parâmetros analisados (bidimensionais, tridimensionais e transporte do canal). No terço cervical, foram encontradas diferenças estatisticamente significantes (p<0,05) entre os sistemas, com maiores alterações entre RC e RCB, em relação aos sistemas PTN e TRS. No terço apical, não houve diferenças estatisticamente significantes (p>0,05) entre os sistemas, em relação aos parâmetros estudados. Pode-se concluir que os sistemas em estudo, independentemente do número de instrumentos, do tipo de movimento, do tipo de tratamento térmico das ligas e das características particulares de cada instrumento, não promoveram alterações na zona crítica apical, e que os instrumentos de maior conicidade promoveram maiores mudanças na morfologia do canal, de acordo com os parâmetros avaliados / The aim of the present study was to analyze, by micro-CT, the two (area and perimeter) and three (volume, surface area and SMI) dimensional changes and root canal transportation in mesial roots of mandibular molars with severe curvature, after the biomechanical procedures performed by four different NiTi, varying the kinematic type and preparation concept, the geometry and design of the instruments and the type of alloy treatment. In this way, the multi-instrument rotary system Protaper Next (PTN); the reciprocating single file system Reciproc (RC); the reciprocating single file system Reciproc Blue (RCB) and the multi-instrument rotary system TRUShape (TRS), were evaluated. Thirty-two lower molars with mesial root curvature angle of 25-50 ° and radius 4 mm were selected and scanned in a micro-CT 1176 (SkyScan, Kontich, Belgium) with 18 m isotropic resolution. After that, the images were reconstructed and analyzed to obtain the initial parameters (pre-instrumentation) of the area and perimeter, and three-dimensional volume, surface area, and SMI, besides of the root canal transportation by means of the centroid values. The specimens were distributed in experimental groups according to the instrumentation system used (n = 8), and after instrumentation, a new microtomographic analysis was performed following the initial standards described. The pre and post instrumentation data were tabulated and then the statistical analysis was performed using ANOVA and post Tukey test, with a significance level of 5%. The results showed that, regardless of the instrumentation system, changes in the two-dimensional and three-dimensional parameters, as well as the root canal transportation were noticed. No statistically significant differences (p> 0.05) were observed between the RC and RCB systems, as well as between the PTN and TRS systems in none of the analyzed parameters (bidimensional, three-dimensional and root canal transportation). In the cervical third, statistically significant differences (p <0.05) were found between the systems, with greater changes to RC and BCR, in relation to the PTN and TRS systems. In the apical third, there were no statistically significant differences (p> 0.05) between the systems, in relation to the parameters studied. It can be concluded that the systems analyzed in this study, independently of the number of instruments, the type of movement, the type of heat treatment of the alloys and the particular characteristics of each instrument, did not promote changes in the critical apical zone, and that the conicity promoted greater changes in the morphology of the canal, according to the evaluated parameters

Orientador: Bruno Watanabe Minto / Banca: Luis Gustavo Gosuen Gonçalves Dias / Banca: Fernando Yoiti Kitamura Kawamoto / Resumo: Objetivou-se neste estudo avaliar a matriz óssea desmineralizada caprina como xenoenxerto em falhas ósseas tibiais de coelhos. Foram utilizados 24 coelhos fêmeas, adultos da raça Nova Zelândia. Uma falha circular monocortical de 6 mm de diâmetro foi criada em ambas as tíbias, no terço proximal da superfície medial. A lesão da tíbia esquerda foi preenchida com 70mg de matriz óssea desmineralizada caprina, enquanto que a direita correspondeu ao controle. Nos períodos pós-operatórios de 15, 30, 60 e 90 dias, os 6 animais de cada grupo foram submetidos à eutanásia e amostras de tíbia proximal foram coletadas. As amostras foram escaneadas por meio de microtomografia computadorizada helicoidal. Analisou-se as características macroscópicas do reparo ósseo, o padrão de preenchimento trabecular e formação de tecido ósseo intralesional. Foram observadas diferenças estatisticamente significativas entre os grupos, para os parâmetros volume ósseo e na relação entre volume ósseo e volume total nos tempos 15, 30 e 90 dias, com maior produção óssea obtida do grupo controle. Evidenciou-se também superioridade estatística do grupo controle em relação ao grupo matriz, para as variáveis superfície óssea, relação entre superfície óssea e superfície total e número de trabéculas, nos tempos 15 e 90 dias, indicando maior superfície óssea do tecido de reparo no grupo controle. A matriz óssea desmineralizada caprina demonstrou ser biotolerável e segura em coelhos, durante 90 dias, comprovando por meio... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The objective of this study was to evaluate the demineralized goat bone matrix as xenograft in tibial bone defects of rabbits. Twenty-four female rabbits, New Zealand adults, were used. A circular monocortical defect of 6 mm in diameter was created in the form of tibias, without proximal third of the medial surface. The case of the boundary case of the case of the demineralized caprine, while a corresponding right to control. On the postoperative days of 15, 30, 60 and 90 days, the six animals were submitted to euthanasia and proximal tibia samples were collected. The samples were scanned by computerized helical microtomography. Analyzed as macroscopic of bone repair, trabecular filling pattern and intralesional bone formation. Statistically significant differences were observed between the groups, so that the volumes were adjusted to the variable between volume and total volume at times 15, 30 and 90 days, with higher bone production in the control group. It was also evidenced the statistical superiority of the control group in relation to the set of matrices, such as the temperature in bone ground, the relation between the bone surface and the total surface and the number of trabeculae, in the 15 and 90 days, control. The demineralized bone matrix demonstrated to be biotolerable and safe in systems, during 90 days, proved by computer microtomography, the beginning of the system installation in 30 days after implantation, as well as a bone bridge formation at 60 days. / Mestre

O presente estudo teve como objetivo analisar, por meio de microtomografia computadorizada, os parâmetros morfológicos das raízes mesiais de molares inferiores, preparados por três sistemas mecanizados: reciprocante (Reciproc - RC), rotatório contínuo (OneShape - OS) e reciprocante/rotatório (Twisted-File Adaptive - TFA), precedidos ou não de pré-alargamento (ProGlider - PG). Foram utilizados trinta molares inferiores, com raiz mesial apresentando ângulo (&ge;25º) e raio de curvatura (&le;2mm) severos e dois canais distintos. Por meio da técnica de amostragem estratificada, os canais foram distribuídos em seis grupos (n=10) conforme protocolo de instrumentação: G1-TFA; G2-RC; G3-OS; G4-PG + TFA; G5 - PG + RC; G6- PG + OS. Após o preparo, foram avaliados os parâmetros bidimensionais (área, perímetro, circularidade, diâmetro maior e menor) nos 5mm apicais e tridimensionais (volume, área de superfície e SMI), bem como o transporte do canal radicular. Os dados obtidos foram analisados estatisticamente por meio do teste ANOVA seguido do complementar de Tukey (&alpha;=0,05). O RC apresentou valores, significantemente, maiores de área, perímetro e diâmetro menor em relação ao TFA (p<0,001), não havendo diferença significante entre os sistemas em relação à circularidade (p=0,056) e diâmetro maior (p=0,477). O aumento do volume do canal proporcionado pelos sistemas RC e OS foram semelhantes entre si (p=0,979) e diferente estatisticamente ao TFA (p<0,001). O aumento da área de superfície promovida pelo RC foi similar ao OS (p=0,254) e diferente estatisticamente ao TFA (p=0,001), não havendo diferença entre os dois últimos sistemas (p=0,075). Os valores de SMI do grupo PG + OS foram significativamente maiores que dos demais grupos (p<0,001). Não houve diferença entre os sistemas em relação ao transporte nos terços apical e médio. Concluiu-se que todos os sistemas avaliados apresentaram alterações morfológicas bi e tridimensionais após o preparo biomecânico. O emprego do sistema ProGlider previamente aos sistemas de instrumentação não influenciou as alterações dos parâmetros avaliados. / The present study aimed to analyze, by computerized microtomography, the morphological parameters of mesial roots of mandibular molars prepared by three mechanized systems: reciprocating (Reciproc - RC), continuous rotating (OneShape - OS) and Twisted - File Adaptive (TFA), preceded or not by pre - enlargement (ProGlider-PG). Thirty teeth were used, with mesial root presenting severe angle (&ge;25º) and a radius of curvature (&le;2mm) and two distinct canals. By means of the stratified sampling technique, the canals were distributed in six groups (n = 10) according to the instrumentation protocol: G1-TFA; G2-RC; G3-OS; G4-PG + TFA; G5 - PG + RC; G6- PG + OS. After preparation, the two-dimensional parameters (area, perimeter, roundness, major and minor diameter) were evaluated in the apical 5mm as well as three-dimensional parameters (volume, surface area and SMI) and root canal transportation. The data were statistically analyzed using the ANOVA test followed by Tukey\'s complement (&alpha; = 0.05). The RC presented significantly larger values of area, perimeter, and minor diameter in relation to the TFA (p<0,001), and there was no significant difference between the systems in relation to the roundness (p=0,056) and major diameter (p=0,477). The increased canal volume provided by the RC and the OS systems were similar to each other (p=0,979) and statistically different from the TFA (p<0,001). The increase of the surface area promoted by the RC was similar to the OS (p=0,254) and statistically different to the TFA (p=0,001), with no difference between the other two systems (p=0,075). The SMI values of the PG + OS group were significantly higher than the other groups (p<0,001). There was no difference between the systems in relation to transport in the apical and middle thirds. It was concluded that all evaluated systems presented bi-dimensional and three-dimensional morphological alterations after biomechanical preparation. The use of the ProGlider system prior to the instrumentation systems did not influence the parameters evaluated.