The objectives of this study are to use phytoremediation ecotechnology to improve the long-term soil pollution contaminated by petroleum and its refined products, and to explore the influence of environmental factors to the effective degradation of TPH.This study is divided into three stages.First, we selected the biofuel crops seeds to test their diesel fuel pollution tolerance.The crops include soybean¡Bsunflower¡Bcanola and corn.This four Taiwanese common energy crops were selected to manually configure three levels of diesel fuel pollution(1000¡B5000¡B10000 mg kg-1)in soil test the seed tolerance experiments.The experimental results in the first stage exhibited that the best energy crop species and non-edible crop(Jatropha),are selected in second phase for contaiminated soil degradation experiment to explore the possible influence of enviromented factors,such as soil moisture¡BpH¡Btotal plate¡Ketc,and to explore the applied fertilizer to increase soil nutrients,whether it will affect the degradation of diesel pollution.Finally, in the third phase, the energy crops were used in the oil-contaminated site to assess their decontamination efficiency. From June 2011 to November 2011, the experimental results shown in the first phase of seed tolerance test, for a period of 30 days showed that the soybean diesel-contaminated soil presented the best tolerance.Although the germination rate was increased with the concentration from 80% to 27%, it showed the best growth conditions.Therefore, in the second phase of test run, the speices of soybean and jatropha were selected prepared with concentrations of 1745¡B6271 and 10072 mg kg-1 dry soil. After 90 day for phytoremediation, soybean group(S) were found that the residual concentrations in soil were measured equal to 524¡B809 and 1913 mg kg-1 dry soil,with the removal rates of 69.97%¡B87.09% and 81.01% respectively.The concentration level of 10000 ppm was found not reach our control standard of 1000 ppm. The soil planted by jatropha(J) showed that residual concentration in soils equial to 303¡B1864 and 4837 mg kg-1 dry soil, with removal rates equal to 82.61%¡B70.27% and 51.98% respectively.Through statistical regression analytical results, the soybean can handle up to a concentration of 5300 mg/kg for diesel, while jatropha can handle up to 2170 mg/kg in this system. Except for the concentration level of 1000 ppm can reach our control standards, the other two groups were found below the control standard. To improve the removal efficieneies, it was suggested that phyto remediation time can be extended.

This study focuses on the Kaohsiung industrial pier sediment survey, assessment and feasibility study of the approach. In this study, field monitoring operations, including the close Salt Water River mouth area of the industrial port (area A), the far Salt Water River mouth area of the industrial port (area B) and for the factories and shipyards at the junction of the terminal area (area C), The sampling of sediments of three core and three surface sediments of area A that used as treating test at laboratory. The survey results show that the industrial pier some heavy metals in the sediment concentration is higher than the quality indicators in the sediment above the limit (ULV), especially copper and zinc. In addition, the concentration of heavy metals of industrial pier area A, B and C of the sediment at least one of them is than current soil control standard. Among them, the frequency of exceeding control standards of copper concentration is the highest, the surface sediments of area A, B and C were about 75%, 42% and 0% respectively, while the core sediments were about 20%, 90% and 15%. These results indicate that the industrial pier sediment required to carry out appropriate pre-treatment to reclamation land to recycling. After investigation, simulation and estimation, the required appropriate treatment sediment in order to landfill volume of industrial pier area A and B (Salt Water River mouth) were approximately 40,000 and 36,400 cubic meters, the total approximately 76,400 cubic meters. Industrial pier is located in the Salt Water River mouth, and therefore withstand the effects of pollutants of the upstream sources flowed in, and than the sediment quality was poor. Sediments were accumulated in the bottom should be removed and sediments at the upstream Salt Water River should be treated too, the remediation and pollution source control for the future to improve the sediment quality is the most important work in Taiwan. In this study, chemical washing and chemical oxidation of the two treatment technology for industrial pier sediment organic pollutants (total petroleum hydrocarbons (TPH) as the target pollutants) to deal with the feasibility test. Sediment to be processed was collected neart the industrial pier, the pH value of approximately 7.1, the moisture content was 43.9%, 20.1% organic matter content, while the particle size composition of mainly fine particles (silt + clay) to about 84.3% handling may be more difficult. The sediments of the TPH concentration of 8,691 mg / kg. Three surfactants Simple Green (SG), Triton X-100 (TX-100) and Tween 80 (TW80) were used at sediment washing test,washing with 60 pv and 5% (v / v) SG could remove 97.3% TPH at the end of the mud; 0.5% (v / v) TX-100 could remove 96.8% TPH; washing with 30 pv, 1% (v / v) TX-100 could remove 94.6% the TPH; washing with 10 pv, 5% (v / v) TX-100 could remove 96.7% TPH; but TW80 leaching ineffective. Oxidation processing, applied 6% H2O2 reaction 180 min, 58.2% of TPH could be removed. Connection of washing and oxidation treatment process, could be removed total of 86% of TPH. The sediment surface morphology before and after treatment were observed by SEM were not significantly different, no surfactant emulsion was left at sediment after treated, this result revealed the connection of washing and oxidation treatment process could remove most of TPH and less harmful to the environment was an available technique.

No description available.

Alternative strategies involving in situ remediation technologies have been developed to assist with property clean up, however, cost-effectiveness and discrepancies in success rates and timeliness continue. The objective of my research was to critically demonstrate the application and usefulness of an in situ remediation technology at a petroleum hydrocarbon impacted site. This project was proposed as part of the research programs: Groundwater Plume Formation and Remediation of Modern Gasoline Fuels in the Subsurface and Enhancing In Situ Bioremediation at Brownfield Sites funded by the Ontario Centres of Excellence for Earth and Environmental Technologies as part of the multiphase project entitled “Enhancing in situ Bioremediation at Brownfield Sites”. This research focused on the demonstration of nitrate-enhanced in situ bioremediation at a decommissioned service station. Petroleum hydrocarbon impacted soil and groundwater is a common occurrence at gasoline distribution facilities, where toxicological effects are known for gasoline constituents of interest such as benzene, toluene, ethylbenzene and total xylenes (BTEX). These chemicals are volatile, readily soluble, and persistent in groundwater. In particular, residual contaminants present in the saturated zone were targeted for remediation as they serve as a long term source of contamination and contribute to mobile vapour phase and dissolved phase plumes. Site investigations characterized the complex hydrogeological conditions and contaminant distribution present in order to effectively design an in situ bioremediation treatment system. The addition of nitrate as a terminal electron acceptor (TEA) to an aquifer enhances in situ biodegradation of petroleum hydrocarbons, by providing the microbes with a sustainable energy source to promote cell maintenance and growth of the microbial population. The remediation strategy involved pulsed injections of remedial solution amended with a conservative bromide (200 mg/L Br-) and reactive nitrate (90 to 265 mg/L NO3-) tracers with the purpose of providing a continuous supply of TEA available to the indigenous microbial populations. Nitrate was selected as an alternative electron acceptor over the thermodynamically favoured O2 because of typical challenges encountered using O2 in bioremediation applications in addition to the existing anaerobic environment. In situ anaerobic degradation of BTEX compound using TEA amendments has been well documented; however benzene is often recalcitrant under denitrification conditions. The results of the Br- tracer breakthrough curves indicate that different preferential flow pathways were established under the transient saturated conditions present at the Site, although the behaviour of the injected remedial slug was generally consistent between the different units and the test solution was ultimately delivered to the target zone. The delivery of the remedial test solution was greatly influenced by the hydrogeological conditions present at the time of injection. The injectate was preferentially transported in the high permeability zone of sandy gravel aquifer Unit 3 under high saturated condition and background hydraulic gradients. However the seasonal decline in groundwater levels and hydraulic gradients resulted in the lower portion of Unit 4 comprised of higher permeable materials being able to transmit the test solution more effectively. Given the variable hydrogeological conditions present at the Site influenced by seasonal effects, the delivery of the remedial solution to target zones containing petroleum hydrocarbons at residual saturation is more effective under reduced saturated conditions. The delivery of TEA amended water to enhance the in situ biodegradation of petroleum contaminants is more effective when the treatment water has an increased residence time in the target remedial zone, attributed to low gradients and groundwater transport velocities at the Site. Longer residence periods enable the indigenous microbes to have increased contact time with the TEA which will be preferentially utilized to degrade the contaminants.   A reducing zone enriched with TEA in the anaerobic aquifer was established following consecutive injections of remedial test solution. A cumulative mass of 4 kg of NO3- was added to the target aquifer during the course of the remedial injections. Evidence demonstrating NO3- utilized as a terminal electron acceptor in the bioremediation of the petroleum-contaminated aquifer include: laboratory microcosm study confirming local indigenous microbial population’s ability to degrade hydrocarbons using NO3- as the TEA in addition to observed decrease in NO3- relative to a conservative Br- tracer and generation of nitrite, an intermediate product in denitrification in the pilot-scale operation. Contaminant mass removal likely occurred as Br- tracer evidence indicates that NO3- was utilized in the study area based on the inference of denitrification rates. Post-injection groundwater sampling indicate declining concentrations of toluene, however long term monitoring is recommended in order to evaluate the success of the remediation activity and assess the potential for rebound. Post-injection soil core results are unable to demonstrate the reduction in individual toluene, let alone BTEXTMB hydrocarbon levels, as a result of insufficient quantities of nitrate delivered to the target zone relative to the significant but heterogeneously distributed residual mass in the subsurface.

abstract: In this work, the vapor transport and aerobic bio-attenuation of compounds from a multi-component petroleum vapor mixture were studied for six idealized lithologies in 1.8-m tall laboratory soil columns. Columns representing different geological settings were prepared using 20-40 mesh sand (medium-grained) and 16-minus mesh crushed granite (fine-grained). The contaminant vapor source was a liquid composed of twelve petroleum hydrocarbons common in weathered gasoline. It was placed in a chamber at the bottom of each column and the vapors diffused upward through the soil to the top where they were swept away with humidified gas. The experiment was conducted in three phases: i) nitrogen sweep gas; ii) air sweep gas; iii) vapor source concentrations decreased by ten times from the original concentrations and under air sweep gas. Oxygen, carbon dioxide and hydrocarbon concentrations were monitored over time. The data allowed determination of times to reach steady conditions, effluent mass emissions and concentration profiles. Times to reach near-steady conditions were consistent with theory and chemical-specific properties. First-order degradation rates were highest for straight-chain alkanes and aromatic hydrocarbons. Normalized effluent mass emissions were lower for lower source concentration and aerobic conditions. At the end of the study, soil core samples were taken every 6 in. Soil moisture content analyses showed that water had redistributed in the soil during the experiment. The soil at the bottom of the columns generally had higher moisture contents than initial values, and soil at the top had lower moisture contents. Profiles of the number of colony forming units of hydrocarbon-utilizing bacteria/g-soil indicated that the highest concentrations of degraders were located at the vertical intervals where maximum degradation activity was suggested by CO2 profiles. Finally, the near-steady conditions of each phase of the study were simulated using a three-dimensional transient numerical model. The model was fit to the Phase I data by adjusting soil properties, and then fit to Phase III data to obtain compound-specific first-order biodegradation rate constants ranging from 0.0 to 5.7x103 d-1. / Dissertation/Thesis / Ph.D. Civil and Environmental Engineering 2012

Esta pesquisa tem por objetivo contemplar as variáveis inerentes aos compostos, mais precisamente hidrocarbonetos totais de petróleo em função de sua disposição no meio ambiente, relacionando o comportamento associado ao seu risco toxicológico e de forma inovativa, verificar a influência de se considerar toda a gama de hidrocarbonetos de petróleo na avaliação de risco, quantificando o erro e incerteza de uma avaliação de risco clássica. O escopo de trabalho contemplou a definição da potenciometria local, definição de contaminantes de interesse, modelagens de transporte e para estes contaminantes e definição do programa de gerenciamento da área em função da inclusão de um modelo de risco toxicológico. A área encontra-se inserida em um pacote sedimentar que confere condutividades hidráulicas entre 10-4 e 10-5 cm/s. O modelo de fluxo é radial, e a velocidade linear de fluxo foi definida entre 0,5 e 8,6 m/ano. Os resultados obtidos nas campanhas sistemáticas de monitoramento indicaram que a grande maioria dos poços apresentou concentrações abaixo dos padrões ambientais. Obteve-se a constatação de contaminação efetiva nas áreas compreendidas pela Oxiteno (OXI) e Petroflex (PTX). As simulações de risco efetuadas somente para os compostos BTEX indicaram valores acima dos limites preconizados pelos órgãos ambientais somente em um cenário hipotético de utilização de água captada em área próxima ao poço PPTX 070 (Petroflex). Os demais cenários apresentam riscos dentro dos limites aceitáveis. A quantificação do risco toxicológico associado a toda a gama de hidrocarbonetos de petróleo apresentou valores mais restritivos do que as avaliações executadas para a mesma amostra quando considerou-se somente os compostos padronizados etilbenzeno, tolueno e xilenos, As variações foram de até duas ordens de grandeza no quociente de risco. A definição de metas de remediação e adoção de um programa de gestão específico deve considerar toda a gama de hidrocarbonetos de petróleo e não somente compostos padronizados. / This research aims to consider all the inherent variables to the compounds due to their disposal in the environment, relating their fate associated to their toxicological risk and verify the influence of considering all the petroleum hydrocarbons range in the risk evaluation, quantifying the uncertainty of a classic risk evaluation. The study scope considered the potenciometric trend of the site, definition of compounds of interest; fate and transport modelling for these compounds and finally the definition of the site management program associated to a model of toxicological risk. The potenciometric map illustrates a radial flow for the groundwater, with hydraulic conductivities ranging from 10-4 to 10-5 cm/s. The lineal flow velocity of groundwater was determined between 0,5 m/year and 8,69 m/year. The results obtained in the systematic monitoring campaigns indicated that the great majority of the wells presented concentrations below the environmental standards. It was verified effective contamination in the areas comprehended by Oxiteno (OXI) and Petroflex (PTX). The risk simulations performed for the BTEX compounds indicated values above the limits accepted by the environmental agencies only for a hypothetical scenery regarding explotation of water nearby the PPTX 070 well area. The other scenaries presented acceptable risk limits. The quantification of the toxicological risk associated to all the petroleum hydrocarbons range presents more restrictive values than the evaluations performed for the same water sample, when it was considered only the standardized compounds ethilbenzene, toluene and xilenes, The variations went up to two greatness orders in the risk quotient. The definition of remediation goals and adoption of a specific management program must consider all the petroleum hydrocarbons range and not only standardized compounds.

Abstract Despite tightened environmental legislation, spillages of petroleum products remain a serious problem worldwide. The environmental impacts of these spillages are always severe and reliable methods for the identification and quantitative determination of petroleum hydrocarbons in environmental samples are therefore needed. Great improvements in the definition and analysis of total petroleum hydrocarbons (TPH) were finally introduced by international organizations for standardization in 2004. This brought some coherence to the determination and, nowadays, most laboratories seem to employ ISO/DIS 16703:2004, ISO 9377-2:2000 and CEN prEN 14039:2004:E draft international standards for analysing TPH in soil. The implementation of these methods, however, usually fails because the reliability of petroleum hydrocarbon determination has proved to be poor. This thesis describes the assessment of measurement uncertainty for TPH determination in soil. Chemometric methods were used to both estimate the main uncertainty sources and identify the most significant factors affecting these uncertainty sources. The method used for the determinations was based on gas chromatography utilizing flame ionization detection (GC-FID). Chemometric methodology applied in estimating measurement uncertainty for TPH determination showed that the measurement uncertainty is in actual fact dominated by the analytical uncertainty. Within the specific concentration range studied, the analytical uncertainty accounted for as much as 68–80% of the measurement uncertainty. The robustness of the analytical method used for petroleum hydrocarbon determination was then studied in more detail. A two-level Plackett-Burman design and a D-optimal design were utilized to assess the main analytical uncertainty sources of the sample treatment and GC determination procedures. It was also found that the matrix-induced systematic error may also significantly reduce the reliability of petroleum hydrocarbon determination. The results showed that strict implementation of the ISO and CEN draft standards is necessary owing to the method dependence of the analyzed parameter. Care should be taken to ensure that the methods used for petroleum hydrocarbon determination are comprehensively validated, and that routine quality control is carried out in order to ensure that the validation conclusions are applicable in the daily work.

The efficacy of BiOWiSHTM-Thai Aqua, a commercially discontinued microbial product, in remediating oil based drill-cutting mud (DCM) was researched in this study. Experimentation was performed directly on DCM and on sand contaminated with oil extracted from DCM. A gas chromatograph-mass spectrometrer and a respirometer were used for analysis of total petroleum hydrocarbons (TPH) and CO2 production respectively. Five experiments were analyzed by TPH extraction and analysis; four experiments were analyzed by respirometric analysis. The specific microcosm conditions tested in the experiments were control, nutrient-only control, and bioaugmentation product. This study concluded that there might be potential for bioaugmentation of TPH using BiOWiSHTM-Thai Aqua. However, a more extensive study including multiple replicates of samples over a longer sampling time period is required to make a conclusion. TPH analysis from the Sand Microcosm Experiment suggested that in seven days, the addition of BiOWiSHTM-Thai Aqua improved TPH removal relative to the control by 89% while the nutrient-only control improved by 58%. Respirometric analysis suggested CO2 respiration of glucose overshadowed CO2 respiration from biodegradation. Thus, major conclusions could not be made from the respirometric analysis.

O Estado de São Paulo apresenta, atualmente, mais de 5.900 áreas contaminadas cadastradas, as quais foram ou devem ser submetidas a um processo de remediação. Os principais contaminantes identificados são os combustíveis automotivos, com destaque para os hidrocarbonetos derivados de petróleo. Dentre as técnicas de remediação existentes, e considerando-se a necessidade de inclusão dos princípios da sustentabilidade aos processos de remediação, destaca-se a estratégia de adição de composto orgânico ao solo contaminado. O composto apresenta grande potencial de biorremediação, pois é capaz de sustentar e promover o crescimento de diversas populações de microrganismos com habilidade de degradar uma série de contaminantes. Nota-se, no Brasil, grande potencial de utilização de composto para a remediação, tanto pelas características climáticas e físico-químicas dos solos quanto pela quantidade de resíduos orgânicos gerados no país. Dessa forma, a presente dissertação tem por objetivo estudar a aplicação de composto orgânico na remediação de solo contaminado por hidrocarbonetos derivados de petróleo, em escala laboratorial. Foi realizada a contaminação artificial de um solo argiloso com óleo diesel e uma mistura de óleo BPF, diesel e querosene, em duas concentrações (10 g/kg e 100 g/kg) e adicionado composto orgânico em duas proporções (1:0,1 e 1:0,5). Os resultados indicaram maiores reduções das concentrações de hidrocarbonetos totais de petróleo ao final do experimento para os tratamentos realizados com óleo diesel, da ordem de 60 % a quase 90 % no período de 91 dias. A presença do composto contribuiu para o aumento dos teores de matéria orgânica, sólidos voláteis, pH e condutividade elétrica dos tratamentos, além da melhoria da estrutura do solo e maior desenvolvimento de populações de microrganismos. Dessa forma, o composto atuou como fonte de energia para os microrganismos autóctones de solo e como fonte de novos microrganismos metabolicamente ativos, contribuindo para o aumento das taxas de biodegradação. Os resultados do presente trabalho indicam que a adição de composto se mostra eficaz na remediação de solos contaminados por hidrocarbonetos de petróleo, promovendo a melhoria das condições físicas, químicas e biológicos do solo e apresentando-se como uma técnica sustentável, com impactos positivos dos pontos de vista ambiental, social e econômico. / The State of São Paulo currently has more than 5,900 registered contaminated sites, which were or have to be submitted to a remediation process. The main contaminants identified are automotive fuels, with emphasis on petroleum-derived hydrocarbons. Among the existent remediation techniques, and considering the need to include sustainability principles to remediation processes, the strategy of adding organic compost to the contaminated soil stands out. The compost presents a great bioremediation potential as it is capable of sustaining and promoting the growth of diverse microorganisms populations, with potential to degrade a series of contaminants. In Brazil, there are great opportunities for the use of compost for the remediation of contaminated soils, both due to the climatic and physical-chemical characteristics of the soils and the amount of organic waste generated in the country. Thus, the present research aims to study the application of compost in the remediation of a hydrocarbon contaminated soil, in laboratory scale. It was performed the artificial contamination of a clay soil with diesel oil and a mixture of bunker oil, diesel and kerosene in two concentrations (10 g/kg and 100 g/kg) and the compost was added in two proportions (1:0.1 and 1:0.5). The results showed greater reductions in the concentrations of total petroleum hydrocarbons at the end of the experiment for treatments with diesel oil, ranging from 60% to almost 90% in a period of 91 days. The presence of compost contributed to an increase in the contents of organic matter and volatile solids, as well as in the values of pH and electrical conductivity. It was also observed an improvement of the soil structure and a greater development of microbial populations. Thus, the compost acted as an energy source for autochthonous soil microorganisms and as a source of new metabolically active organisms, contributing to the increase of biodegradation rates. The results of the present work indicate that the addition of compost is effective in the remediation of petroleum hydrocarbons contamination, promoting the improvement of the soil physical, chemical and biological conditions and being a sustainable technique with positive impacts from the environmental, social and economic points of view.

Os Planctomycetes são bactérias que possuem características peculiares, ainda pouco conhecidas. São bactérias de difícil cultivo, sendo descritas em diversos ambientes, mas frequentemente isoladas de ambientes marinhos, principalmente em associações com algas. Aqui apresentamos um amplo estudo deste grupo em solos de manguezais, e reportamos de maneira inovadora o isolamento dessas bactérias. A comparação das comunidades de Planctomycetes em manguezais com diferentes históricos de contaminação permitiu fazer inferências sobre a resposta da comunidade ao ambiente. A análise de sequências pertencentes a este filo, obtidas a partir de amostras dos manguezais - tanto sequências do gene 16S DNAr ou sequências metagenômicas - permitiu inferir sobre a diversidade e as funções destes grupos nos solos dos manguezais estudados. Destacam-se dentre estas o aumento da biodiversidade deste grupo em áreas contaminadas, e as evidências de sua participação na degradação de xenobióticos (demonstrada por predição metagenômica baseada em biblioteca de 16S rDNA, e análise de sequencias metagenômicas). Com estes resultados, foram encontrados em dados metagenômicos, a ocorrência de genes envolvidos na biodegradação de compostos intermediários centrais das vias de degradação. Adicionalmente, mesmo com a dificuldade no cultivo de membros desse grupo, foram obtidos 43 isolados afiliados filogeneticamente principalmente às espécies Blastopirellula marina, Rhodopirellula baltica e Planctomycetes sp. e Pirellula. Foram selecionados dois isolados (Rhodopirellula sp. MGV e Rhodopirellula baltica BR-MGV) para estudos genômicos e metabólicos (via análise de consumo de diferentes fontes de carbono-Biolog). Além disso, foi realizado teste de degradação de hidrocarbonetos com os 43 isolados aferindo as respostas ao contato com contaminantes como hexadecano, naftaleno, fenantreno e fenol. Cinco isolados mostraram a capacidade em degradar três diferentes hidrocarbonetos exceto fenantreno. Estes isolados (Blastopirellula cremea Hex-1 MGV, Blastopirellula marina Nap-Phe MGV, Blastopirellula cremea Hex-2 MGV, Blastopirellula cremea Hex PRIS-MGV e Blastopirellula marina Nap PRIS-MGV) foram enviados a uma análise genômica e suas anotações indicaram a presença de genes envolvidos em vias de degradação de hidrocarbonetos corroborando com o teste realizado em laboratório de degradação dos hidrocarbonetos. Além disso, foi observada através das anotações dos genomas desses microrganismos a ocorrência de síntese de metabólitos secundários, sendo os principais terpenos, bacteriocinas e resorcinol. / Planctomycetes are bacteria with peculiar characteristics and still little known. They are bacteria of difficult cultivation, being described in diverse environments, but often isolated from marine environments, mainly in associations with algae. Here we present an extensive study of this group in mangrove soils and report in an innovative way the isolation of these bacteria. The comparison of the communities of Planctomycetes in mangroves with different contamination histories allowed to make inferences about the response of the community to the environment. The analysis of sequences from this phylum, obtained from samples of the mangroves - both sequences of the 16S DNAr gene or metagenomic sequences - allowed to infer about the diversity and the functions of these groups in the mangrove soils studied. The highlights are the increase of this group\'s biodiversity in contaminated areas, and evidence of its participation in the degradation of xenobiotics (demonstrated by 16S rDNA library-based metagenomic prediction and metagenomic sequence analysis). With these results, the occurrence of genes involved in the biodegradation of central intermediates of the degradation pathways was found in metagenomic data. In addition, even with the difficulty in the cultivation of members of this group, 43 isolates belonging to this phylum were obtained phylogenetically, mainly the species Blastopirellula marina, Rhodopirellula baltica and Planctomycetes sp. and Pirellula. Two isolates (Rhodopirellula sp. MGV and Rhodopirellula baltica BR-MGV) were selected for genomic and metabolic studies (via consumption analysis of different sources of carbon-Biolog). Furthermore, a hydrocarbon degradation test was performed with this library of 43 isolates, assessing the responses to contact with contaminants such as hexadecane, naphthalene, phenanthrene and phenol. Five isolates showed the ability to degrade three different hydrocarbons except phenanthrene. These isolates (Blastopirellula cremea Hex-1 MGV, Blastopirellula marina Nap-Phe MGV, Blastopirellula cremea Hex-2 MGV, Blastopirellula cremea Hex PRIS-MGV and Blastopirellula marina Nap PRISMGV) were sent to a genomic analysis and their notes indicated the presence of genes involved in hydrocarbon degradation pathways, corroborating with the laboratory test for hydrocarbon degradation. In addition, the synthesis of secondary metabolites was evaluated through the annotations of the genomes of these microorganisms, being the main terpenes, bacteriocins and resorcinol.