Since the early Eighteenth Century the significance of nectar secretion has been debated. Some have reasoned that it is reabsorbed into the plant and used as energy for the developing embryo while others believe it is a waste product which attracts pollinating insects. It has been suggested (Brink and Cooper, 1947) that the nutrient supply to developing ovules is a major factor in the development of seed. Also, alfalfa fields pollinated by pollen-collecting bees produce greater seed yields than those pollinated by nectar collectors (Bohart, Nye, and Levin, 1955). It is a well-known fact that more flowers are tripped by the pollen collectors, but it is debatable whether the nectar not taken by the pollinators has any influence on the quality or amount of seed set. It is then necessary to find whether the nectar not taken by bees is used by the plant before studies can be made to show its effect on seed and forage yields. The object of this study was to treat flowers of several families with C14 labeled sucrose and determine by the use of autoradiograms if sugars can be absorbed by nectaries, where the sugars are translocated, and when absorption takes place.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19024号 / 農博第2102号 / 新制||農||1030(附属図書館) / 学位論文||H27||N4906(農学部図書室) / 31975 / 京都大学大学院農学研究科食品生物科学専攻 / (主査)教授 伏木 亨, 教授 保川 清, 教授 金本 龍平 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19776号 / 農博第2172号 / 新制||農||1041(附属図書館) / 学位論文||H28||N4992(農学部図書室) / 32812 / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 金山 公三, 教授 矢野 浩之, 教授 五十田 博 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・論文博士 / 博士(人間健康科学) / 乙第13064号 / 論人健博第3号 / 新制||人健||3(附属図書館) / (主査)教授 高桑 徹也, 教授 三谷 章, 教授 横出 正之 / 学位規則第4条第2項該当 / Doctor of Human Health Sciences / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第20766号 / 農博第2249号 / 新制||農||1054(附属図書館) / 学位論文||H29||N5086(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 金山 公三, 教授 矢野 浩之, 教授 吉村 剛 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

No description available.

Opioid use disorder is a chronic, relapsing disease, with more than 85% of individuals experiencing a relapse episode within one year. One common reason patients relapse is due to intense cravings, which are defined as the compulsive urge to use the drug. In fact, craving was recently added to the DSM criteria for substance use disorder diagnosis. Counterintuitively, cravings intensify over the course of extended abstinence, especially in response to drug-paired cues, a phenomenon known as “incubation of craving”. This contributes to difficulty in maintaining long-term sobriety. The mesocorticolimbic reward pathway facilitates self-administration and cue-induced incubation of craving for drugs of abuse and natural rewards, such as sucrose. In particular, the shell sub-region of the nucleus accumbens is a critical brain region involved in context/cue-mediated reward seeking. In the experiments described here, we utilized an incubation of craving model, in which male and female rats self-administered opioids (morphine or heroin) or sucrose for 10 days. Sucrose served as an important control for delineating drug-induced changes from those caused in response to natural rewards, which are not the intended target of potential treatments. Reward delivery was paired with a cue light that was later used to elicit craving. After self-administration, rats underwent brief (one day) or extended (30 days) forced abstinence. One or 30 days later, they were returned to the chambers for a “cue test”, in which responses on the previously reward-associated lever triggered cue presentation, but no contingent reward. We used this model to further delineate behavioral and affective changes that accompany increased opioid craving in late abstinence, as well as molecular alterations underlying craving in rats that did not undergo a cue test. We found an opioid-specific behavioral signature in which peak opioid craving is accompanied by decreased grooming and hyperactivity in both sexes. We tracked the female estrous cycle throughout, as these fluctuations in reproductive hormones (akin to the menstrual cycle) are shown to affect cocaine- and nicotine-related behaviors. We found no differences between females in different phases of the estrous cycle in terms of self-administration, nor craving. RNA sequencing of the nucleus accumbens shell revealed robust changes in gene expression that occurred across extended abstinence, though the genes themselves were altered in a sex- and reinforcer-specific manner. In general, we found many behavioral and molecular changes that were unique to sex and reinforcer (sucrose versus opioids). This is promising in terms of identifying opioid-specific targets that are unlikely to affect the natural reward system in both sexes. Changes in gene expression in the brain are mediated in part by epigenetic processes that influence access of transcriptional machinery to DNA. Acetylation of histone tails, the proteins around which DNA is wrapped and packaged in the nucleus, have been identified as permissive marks that facilitate long-lasting changes in transcriptomics in response to environmental insults. Opioids promote increased acetylation, which may contribute to some of the reported changes here. We tested the efficacy of JQ1, a treatment that interferes with the read-out of opioid-induced acetylated marks, at attenuating heroin self-administration. When administered as an intracerebroventricular microinjection on self-administration day 11, JQ1 had no effect on subsequent heroin taking in either sex, suggesting that it may not be suitable as a systemic treatment at the dose given. These studies lay the groundwork for future studies to administer other treatments throughout abstinence, based on the opioid-specific genes and pathways identified here, to reduce cue-induced heroin craving and the accompanying suite of behaviors in males and females. / Psychology

The biological treatment of wastewaters from an industry was studied. Among the more important wastewater constituents of concern were high levels of suspended solids, due to graphite and nitrocellulose, the solvents, ethanol and acetone, and nitroglycerine (NG). The goal of this project was divided into four objectives. The impacts of graphite on a microbial population were evaluated. Sequencing batch reactors (SBRs) were used to monitor the effects of graphite on mixed liquor suspended solids (MLSS), removal of soluble chemical oxygen demand (sCOD), and specific oxygen uptake rates (sOUR). Graphite appeared to have no adverse effect on the microbes. The potential benefits of adding sucrose, nitrogen, and phosphorus to SBRs were evaluated. The MLSS was maintained at 1,250 mg/L, similar to the microbial population in the suspended growth system at the industry. Sucrose addition increased the sCOD removals and sOUR. No direct effect was observed with the addition of nitrogen and phosphorus. The treatability of acetone and ethanol was studied through sOUR and batch testing to determine bacterial response to solvents. Both solvents were utilized by the microbes. The concentrations tested proved to be beneficial, not inhibitory. Ethanol and a 50/50 mixture of acetone and ethanol were more viable substrates than acetone. NG treatability was examined under anoxic and aerobic conditions in SBRs and batch biological reactors. NG degradation occurred under anoxic conditions, but was more favorable in aerobic environments. NG was degraded in all SBR tests to below detection limit (0.5 mg/L); therefore, the optimal treatment could not be determined. / Master of Science

The purpose of this thesis is to provide a critical analysis of research findings about nonpharmacological techniques used independently of pharmacological techniques to prevent or reduce procedural pain for infants in the neonatal intensive care unit (NICU). A synthesis of the current research related to nonpharmacological pain relief techniques for infants was conducted for this thesis. Nonpharmacological interventions reviewed in this study include: Kangaroo Care, swaddling, facilitated tucking, positioning, music, non-nutritive sucking and sucrose. An interdisciplinary review of the research was performed using the interdisciplinary databases Cumulative Index of Nursing and Allied Health, PubMED, and PsychINFO. Inclusion criteria for this thesis consists of research focused on preterm neonates (born less than] 37 weeks gestational age) in the neonatal intensive care unit, the use of nonpharmacological interventions for procedural analgesia, peer reviewed articles, and those written in the English language. This study excludes full term neonates due to the significant number of preterm neonates in the NICU. A total of 18 studies were included in this review. All interventions except for positioning show statistically significant evidence to support their use to reduce procedural pain in preterm neonates. Findings of this thesis may promote further studies and exploration into this field. In addition, this thesis establishes the role of the nurse in providing pain relief for infants in the NICU, as well as provide for enhancement of interdisciplinary care amongst other health care providers.

No description available.