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Effects of dietary protein and fibre of nitrogen excretion patterns in swineZervas, Symeon 25 May 2012
<p>Successful management of nitrogen (N) excretion is important for sustainable pork
production. Two experiments were conducted and their objectives were to study the
effect of dietary protein and fibre on N excretion patterns and to relate plasma urea (PU)
to urinary N excretion.</p>
<p>In the first experiment, three dietary protein contents (high, 197; medium, 169;
low, 138 g kg<sup>-1</sup>
) and two levels of fibre (low and high) were tested. Diets (wheat, barley,
soybean meal; oat-hulls as the fibre source) were formulated to an equal digestible
energy (DE) content (3.25 Meal DE kg<sup>-1</sup>) and at least 2.18 g digestible lysine per Meal
DE, and were supplemented with Lys, Met, Trp, Thr, Ile, or Val. Pigs (32 ± 3.4 kg; n =
42) were housed in confinement-type metabolism crates for 19 d. On d 10 or 11,
catheters were installed by cranial vena cava venipuncture. Daily feeding rates were
adjusted to three times maintenance and daily rations were halved to two equal meals.
Faeces and urine were collected from d 15 to 19. Five blood samples were collected in
two-h intervals on d 16 and 19. Faecal N, urinary N, and total N excretion were reduced
linearly with a reduction of dietary protein content (P < 0.001). Reduction was greater
for urinary (48%) and total N excretion (40%) than for faecal N (23%) excretion. The
ratio of urinary N to faecal N was reduced linearly with a reduction of dietary protein
content (P < 0.001). Retention of N (g d<sup>-1</sup>) was reduced linearly but N retention as
percentage of intake was increased linearly with a reduction of dietary protein content (P
< 0.001). Addition of oat hulls did not affect N excretion patterns (P > 0.10). Dietary
treatments did not affect average daily gain (ADG) or feed efficiency (P > 0.1 0). Plasma
urea increased after feeding, peaked at 4 h and then decreased toward pre-feeding
concentrations. A linear relationship of urinary N to PU concentration (R<sup>2</sup> = 0.66) was
observed.</p>
<p>In the second experiment, effects of dietary protein content (high, 185; and low,
157 g kg<sup>-1</sup>) and fibre sources on N excretion patterns were studied in a 2x3 factorial
arrangement. The three fibre sources were control, soybean hulls (SH; 15% ), and sugar
beet pulp (SBP; 20%). Diets were formulated to 3.3 Meal DE kg<sup>-1</sup> and 2.4 g digestible
Lys per Meal DE, and were supplemented with Lys, Met, Trp, Thr, lie, or Val. Pigs
(30.5 ± 3 kg; n = 36) were housed in confinement-type metabolism crates, with restricted access to feed (3 x maintenance DE) from d 1 to 18, and free access to feed
from d 19 to 26. Faeces and urine were collected from d 15 to 18 and d 23 to 26, and
blood samples on d 17 and 25. With restricted access to feed, faecal N (as % of N
intake) was increased 3 percentage units for low compared to high protein content, and
increased 4 percentage units for SH and 6.5 percentage units for SBP compared to
control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high
protein content, and reduced 9 percentage units for SBP compared to control (P < 0.05).
Retention of N (g d<sup>-1</sup>) was reduced 12% for low compared to high protein content (17.9
compared to 20.4 g d<sup>-1</sup>
; P < 0.05), and was similar among fibre treatments (P > 0.10).
With free access to feed, faecal N (as % of N intake) was increased 2.5 percentage units
for low compared to high protein content, and increased 5 percentage units for SH and 9
percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5
percentage units for low compared to high protein content, and reduced 9 percentage
units for SH and 10 percentage units for SBP compared to control (P < 0.05). Retention
of N (g d<sup>-1</sup>) was similar for dietary protein content (30.5 g d<sup>-1</sup>
; P > 0.10), and reduced for
SH (27 g d<sup>-1</sup>; P < 0.05) compared to control (31 g d<sup>-1</sup>), while was intermediate for SBP
(29 g d<sup>-1</sup>). A linear relationship of urinary N to PU concentration was observed with
both restricted (R<sup>2</sup> = 0.66) and free access to feed (R<sup>2</sup>= 0.71).</p>
<p>Reduction of dietary protein content is an efficient way to reduce total and
urinary N excretion, which may reduce ammonia emission. Inclusion of fibre sources
high in fermentable fibre shifted N excretion from urine N to faeces N while fibre
sources resistant to fermentation did not have any effects on N excretion patterns.
Combined effects of dietary protein content and fibre reduced urinary N excretion
further than single effects. Level of feed intake is an important consideration when
effectiveness of a nutrient management strategy is studied. Diets with a low protein
content and containing synthetic AA should be studied using pigs with free access to
feed to verify that N retention is maintained. A linear relationship of urinary N to PU
concentration was observed under both restricted and free access to feed indicating that
urinary N excretion could be predicted from PU concentration.</p>
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152 |
Effects of dietary protein and fibre of nitrogen excretion patterns in swineZervas, Symeon 25 May 2012 (has links)
<p>Successful management of nitrogen (N) excretion is important for sustainable pork
production. Two experiments were conducted and their objectives were to study the
effect of dietary protein and fibre on N excretion patterns and to relate plasma urea (PU)
to urinary N excretion.</p>
<p>In the first experiment, three dietary protein contents (high, 197; medium, 169;
low, 138 g kg<sup>-1</sup>
) and two levels of fibre (low and high) were tested. Diets (wheat, barley,
soybean meal; oat-hulls as the fibre source) were formulated to an equal digestible
energy (DE) content (3.25 Meal DE kg<sup>-1</sup>) and at least 2.18 g digestible lysine per Meal
DE, and were supplemented with Lys, Met, Trp, Thr, Ile, or Val. Pigs (32 ± 3.4 kg; n =
42) were housed in confinement-type metabolism crates for 19 d. On d 10 or 11,
catheters were installed by cranial vena cava venipuncture. Daily feeding rates were
adjusted to three times maintenance and daily rations were halved to two equal meals.
Faeces and urine were collected from d 15 to 19. Five blood samples were collected in
two-h intervals on d 16 and 19. Faecal N, urinary N, and total N excretion were reduced
linearly with a reduction of dietary protein content (P < 0.001). Reduction was greater
for urinary (48%) and total N excretion (40%) than for faecal N (23%) excretion. The
ratio of urinary N to faecal N was reduced linearly with a reduction of dietary protein
content (P < 0.001). Retention of N (g d<sup>-1</sup>) was reduced linearly but N retention as
percentage of intake was increased linearly with a reduction of dietary protein content (P
< 0.001). Addition of oat hulls did not affect N excretion patterns (P > 0.10). Dietary
treatments did not affect average daily gain (ADG) or feed efficiency (P > 0.1 0). Plasma
urea increased after feeding, peaked at 4 h and then decreased toward pre-feeding
concentrations. A linear relationship of urinary N to PU concentration (R<sup>2</sup> = 0.66) was
observed.</p>
<p>In the second experiment, effects of dietary protein content (high, 185; and low,
157 g kg<sup>-1</sup>) and fibre sources on N excretion patterns were studied in a 2x3 factorial
arrangement. The three fibre sources were control, soybean hulls (SH; 15% ), and sugar
beet pulp (SBP; 20%). Diets were formulated to 3.3 Meal DE kg<sup>-1</sup> and 2.4 g digestible
Lys per Meal DE, and were supplemented with Lys, Met, Trp, Thr, lie, or Val. Pigs
(30.5 ± 3 kg; n = 36) were housed in confinement-type metabolism crates, with restricted access to feed (3 x maintenance DE) from d 1 to 18, and free access to feed
from d 19 to 26. Faeces and urine were collected from d 15 to 18 and d 23 to 26, and
blood samples on d 17 and 25. With restricted access to feed, faecal N (as % of N
intake) was increased 3 percentage units for low compared to high protein content, and
increased 4 percentage units for SH and 6.5 percentage units for SBP compared to
control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high
protein content, and reduced 9 percentage units for SBP compared to control (P < 0.05).
Retention of N (g d<sup>-1</sup>) was reduced 12% for low compared to high protein content (17.9
compared to 20.4 g d<sup>-1</sup>
; P < 0.05), and was similar among fibre treatments (P > 0.10).
With free access to feed, faecal N (as % of N intake) was increased 2.5 percentage units
for low compared to high protein content, and increased 5 percentage units for SH and 9
percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5
percentage units for low compared to high protein content, and reduced 9 percentage
units for SH and 10 percentage units for SBP compared to control (P < 0.05). Retention
of N (g d<sup>-1</sup>) was similar for dietary protein content (30.5 g d<sup>-1</sup>
; P > 0.10), and reduced for
SH (27 g d<sup>-1</sup>; P < 0.05) compared to control (31 g d<sup>-1</sup>), while was intermediate for SBP
(29 g d<sup>-1</sup>). A linear relationship of urinary N to PU concentration was observed with
both restricted (R<sup>2</sup> = 0.66) and free access to feed (R<sup>2</sup>= 0.71).</p>
<p>Reduction of dietary protein content is an efficient way to reduce total and
urinary N excretion, which may reduce ammonia emission. Inclusion of fibre sources
high in fermentable fibre shifted N excretion from urine N to faeces N while fibre
sources resistant to fermentation did not have any effects on N excretion patterns.
Combined effects of dietary protein content and fibre reduced urinary N excretion
further than single effects. Level of feed intake is an important consideration when
effectiveness of a nutrient management strategy is studied. Diets with a low protein
content and containing synthetic AA should be studied using pigs with free access to
feed to verify that N retention is maintained. A linear relationship of urinary N to PU
concentration was observed under both restricted and free access to feed indicating that
urinary N excretion could be predicted from PU concentration.</p>
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Kemin bakom framtidens avgasrening : En studie av ureasönderfall under kvävgasatmosfär / Chemistry behind future eftertreatmentLe, Tan January 2011 (has links)
The purpose of this work was to provide a better understanding of urea’s decomposition and byproduct formation in an SCR system on heavy trucks. In my experimental setup with TGA-DSC-FTIR (a combination of two thermal analysis methods and a method for gas phase detection), an FTIR method for urea in the gas phase was developed for the qualitative and quantitative determination of urea and its decomposition products. Chemicals such as urea, biuret, cyanuric acid and melamine of p.a. quality were used in this method development. Beforehand, there was no FTIR method available to detect these substances; hence, the aim of this work was to develop an FTIR method to understand the degradation chain of urea. The combination of TGA and DSC was used for analysis of different samples, where urea, biuret, cyanuric acid and melamine in varying amounts have been weighted in for various experiments in order to study the temperature at which a phase transition or reaction occurs, i.e. the temperature at which substances begin to melt, vaporize, decompose and react. In combination with FTIR, information was obtained for the appearance of substances at various temperatures. With FTIR, we have been able to develop unique infrared spectra of substances and along with weight loss in TGA the calibration of different substances has been achieved. These calibrations have been combined together to develop an FTIR method, which has been used for detection of the substances in the ongoing study of the reaction pathways. In this study we also investigated the degradation chain of urea in the presence of metals. Austenitic and ferritic silencer materials with different surface roughness were analyzed to study whether the metals have a catalytic function or effect on the byproduct formation. Those experiments have shown that a higher amount of urea was decomposed in contact with metal surface, i.e. a larger amount of NH3 and HNCO was formed. Biuret studies in the presence of metals appeared to give a higher formation of urea over the rougher surfaces (a larger amount of biuret was decomposed over the rougher surfaces), while experiments with cyanuric acid revealed a higher HNCO formation over ferrite than over austenite, i.e. a larger amount of cyanuric acid was decomposed. By the chosen method, used in FTIR in combination with TGA-DSC, the following important reactions have been demonstrated: Biuret decomposed to urea and HNCO; Urea decomposed into HNCO and NH3; formation of cyanuric acid from the decompositions of urea and biuret and finally decomposition of cyanuric acid into HNCO at a higher temperature.
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The effect of urea and related compounds on the mechanical properties of paperFisher, Henry D. 01 January 1951 (has links)
No description available.
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155 |
Computational studies of protein stabilization and denaturation by small molecules /Bennion, Brian James. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 183-205).
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156 |
The effect of urea and related compounds on the mechanical properties of paperFisher, Henry D., January 1951 (has links) (PDF)
Thesis (Ph. D.)--Institute of Paper Chemistry, 1951. / Includes bibliographical references (p. 185-188).
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157 |
Metabolic engineering of industrial yeast strains to minimize the production of ethyl carbamate in grape and Sake wineDahabieh, Matthew Solomon 11 1900 (has links)
During alcoholic fermentation Saccharomyces cerevisiae metabolizes L-arginine to ornithine and urea. S. cerevisiae can metabolize urea through the action of urea amidolyase, encoded by the DUR1,2 gene; however, DUR1,2 is subject to nitrogen catabolite repression (NCR) in the presence of high quality nitrogen sources during fermentation. Being cytotoxic at high concentrations, urea is exported into wine where it spontaneously reacts with ethanol, and forms the carcinogen ethyl carbamate (EC).
Urea degrading yeast strains were created by integrating a linear cassette containing the DUR1,2 gene under the control of the S. cerevisiae PGK1 promoter and terminator signals into the URA3 locus of the Sake yeast strains K7 and K9. The ‘self-cloned’ strains K7EC- and K9EC- produced Sake wine with 68% less EC. The Sake strains K7EC- and K9EC- did not efficiently reduce EC in Chardonnay wine due to the evolutionary adaptation of said strains to the unique nutrients of rice mash; therefore, the functionality of engineered yeasts must be tested in their niche environments as to correctly characterize new strains.
S. cerevisiae possesses an NCR controlled high affinity urea permease (DUR3). Urea importing yeast strains were created by integrating a linear cassette containing the DUR3 gene under the control of the PGK1 promoter and terminator signals into the TRP1 locus of the yeast strains K7 (Sake) and 522 (wine). In Chardonnay wine, the urea importing strains K7D3 and 522D3 reduced EC by 7% and 81%, respectively; reduction by these strains was equal to reduction by the urea degrading strains K7EC- and 522EC-. In Sake wine, the urea degrading strains K7EC- and 522EC- reduced EC by 87% and 84% respectively, while the urea importing strains K7D3 and 522D3 were significantly less capable of reducing EC (15% and 12% respectively). In Chardonnay and Sake wine, engineered strains that constitutively co-expressed DUR1,2 and DUR3 did not reduce EC more effectively than strains in which either gene was expressed solely. Uptake of 14C-urea under non-inducing conditions was enhanced in urea importing strains; parental strains failed to incorporate any 14C-urea thus confirming the functionality of the urea permease derived from the integrated DUR3 cassette.
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The effect of supplementation of alfalfa hay or urea on intake digestibility and rumen fermentation of sheep fed timothy hay /Delaquis, Annick Marie. January 1987 (has links)
No description available.
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159 |
Triple superphosphate and urea effects on availability of nutrients in the fertilizer band for soybean (Glycine max L.) growth with emphasis on molybdenumYusran, Fadly Hairannoor January 1993 (has links)
Fertilizer applications of urea and triple superphosphate (TSP) may affect availability of plant nutrients in the soil through alteration of soil pH and sorption-displacement effects. The objectives of this experiment were to evaluate urea and TSP effects on nutrient availability to soybean (Glycine max L.). Field experiments were carried out on three Quebec soils; a Chicot sandy clay loam (Gleyed Melanic Brunisol), an Ormstown silty clay loam (Luvic Gleysol) and a Ste. Rosalie clay (Humic Gleysol). Three levels of TSP (0, 40, 80 kg $ rm P sb2O sb5 ha sp{-1}),$ and three levels of urea (0, 25, 50 kg N ha$ sp{-1})$ were incubated in the field and sampled at 4, 8, 12, and 16 weeks. Added TSP increased extractable P and decreased NO$ sb3$-N. Overall, alterations in nutrients other than N and P with added TSP or urea were not agronomically significant. There was increased concentration of N, P and Mo in soybean in some soils due to TSP application. Added urea increased Mg concentration in soybean. The concentration and uptake of Mo was positively correlated with soil extractable P and Mg. Consequently, application of TSP and urea together improved Mo uptake in the Chicot soil, while in slightly acid soils, Ormstown and Ste. Rosalie, TSP alone increased Mo uptake.
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The potential applications of microencapsulated urease and zirconium phosphate for the removal of urea in uraemia /Wolfe, Elizabeth Anne. January 1985 (has links)
No description available.
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