Background Spinal pain is one of humanity’s most frequent complaints with high costs for the individual and society, and is commonly related to spinal disorders. There are many origins behind these disorders e.g., trauma, disc hernia or of other organic origins. However, for many of the disorders, the origin is not known. Thus, more knowledge is needed about how pain affects the neck and neural function in pain affected regions. The purpose of this dissertation was to improve the medical examination of patients suffering from chronic whiplash-associated disorders or other pain related neck-disorders. Methods A new assessment tool for objective movement analysis was developed. In addition, basic aspects of proprioceptive information transmission, which can be of relevance for muscular tension and pain, are investigated by studying the coding of populations of different types of sensory afferents by using a new spike sorting method. Both experiments in animal models and humans were studied to accomplish the goals of this dissertation. Four cats where were studied in acute animal experiments. Mixed ensembles of afferents were recorded from L7-S1 dorsal root filaments when mechanical stimulating the innervated muscle. A real-time spike sorting method was developed to sort units in a multi-unit recording. The quantification of population coding was performed using a method based on principal component analysis. In the human studies, 3D neck movement data were collected from 59 subjects with whiplash-associated disorders (WAD) and 56 control subjects. Neck movement patterns were identified by processing movement data into parameters describing the rotation of the head for each subject. Classification of neck movement patterns was performed using a neural network using processed collected data as input. Finally, the effect of marker position error on the estimated rotation of the head was evaluated by computer simulations. Results Animal experiments showed that mixed ensembles of different types of afferents discriminated better between different muscle stimuli than ensembles of single types of these afferents. All kinds of ensembles showed an increase in discriminative ability with increased ensemble size. It is hypothesized that the main reason for the greater discriminative ability might be the variation in sensitivity tuning among the individual afferents of the mixed ensemble will be larger than that for ensembles of only one type of afferent. In the human studies, the neural networks had a predictivity of 0.89, a sensitivity of 0.90 and a specificity of 0.88 when discriminating between control and WAD subjects. Also, a systematic error along the radial axis of the rigid body added to a single marker had no affect on the estimated rotation of the head. Conclusion The developed spike sorting method, using neural networks, was suitable for sorting a multiunit recording into single units when performing neurophysiological experiments. Also, it was shown that neck movement analysis combined with a neural network could build the basis of a decision support system for classifying suspected WAD or other pain related neck-disorders.

Aerospace Engineering / The aim of this thesis is to explore the high strain rate behavior of metallic specimens using electromagnetic inductive loading as the means to inflict the required high strain rate deformation on laboratory scale specimens, allowing for controlled, repeatable experiments to be performed. Three separate experiments were designed and performed, using helical and spiral coils as the sources of radial and unidirectional loading. The first experiment evaluated the effect of applying a polymer coating on 30.5 mm diameter, Al 6061- O tube samples, in two lengths, 18 and 36 mm. The expanding tube experiment was used to apply a radial loading on the specimens and record the event. Several optical techniques were then used to evaluate the behavior of the samples. Coatings of polyurea and polycarbonate were used. It was observed that the polycarbonate coating seemed to have a more profound effect on the behavior of the metal, by applying a larger restraining pressure on the tube surface during the expansion process, and thereby modifying the stress state of the specimen. The second experiment looked to design an experimental arrangement to test the plane strain, high strain rate behavior of Al 6061-O tubes of different lengths. A 112 mm long solenoid was designed and manufactured, and testing was performed on 30.5 mm diameter Al 6061-O tubes in lengths of 50, 70 and 90 mm. It was observed that the coil behaved similar to shorter ones at low voltages and that the longer the specimen used, the more its deformation path approached a plane strain condition. Finally, a third experiment was performed to develop an experiment to accelerate a plate to high linear velocities, as a means to evaluate the use of a flat spiral coil as the driver for future experiments based upon electromagnetic inductive loading. A prototype coil was manufactured and installed into a converted expanding tube experimental setup. Three samples were tested in several sizes, and materials: aluminum and steel. Speeds in the range of 45 to 251 m/s were obtained, validating the apparatus as a viable method to provide a unidirectional loading. / text

No description available.

The @lab is a group dedicated to the research and development of electronic textiles for architectural applications; this thesis presents the structural analyses performed by the author to improve the @lab’s projects. Also included are three investigations performed by the author that pertain to smart material applications in responsive architecture and textiles. The first investigation evaluated the feasibility of using piezoelectric materials to harvest power from human foot traffic; overall, it was determined to not be feasible. The second investigation experimentally tested how six parameters of shape memory alloy spring actuators affect their reaction times and stroke; all six parameters affected the reaction times and/or stroke. The third investigation experimentally tested how three parameters of superelastic SMA springs influence their stiffness and resonant frequencies; overall, it was found that traditional spring mechanics can be used to predict their behavior providing the internal stress does not reach the upper plateau stress.

With strict environmental regulations, gas turbine emissions have been heavily constrained. This requires operating conditions wherein thermo-acoustic flame instabilities are prevalent. During this process the combustor acoustics and combustion heat release fluctuations are coupled and can cause severe structural damage to engine components, reduced operability, and inefficiency that eventually increase emissions. In order to develop an engine without these problems, there needs to be a better understanding of the physics behind the coupling mechanisms of this instability. Among the several coupling mechanisms, the “velocity coupling” process is the main focus of this thesis. The majority of literature has treated axisymmetric disturbance fields which are typical of longitudinal acoustic forcing and axisymmetric excitation of ring vortices. Two important non-axisymmetric disturbances are: (1) transverse acoustics, in the case of circumferential modes of a multi-nozzle annular combustor and (2) helical flow disturbances, seen in the case of swirling flow hydrodynamic instabilities. With significantly less analytical treatment of this non-axisymmetric problem, a general framework is developed for three-dimensional swirl-stabilized flame response to non-axisymmetric disturbances. The dynamics are tracked using a level-set based G-equation applicable to infinitely thin flame sheets. For specific assumptions in a linear framework, general solution characteristics are obtained. The results are presented separately for axisymmetric and non-axisymmetric mean flames. The unsteady heat release process leads to an unsteady volume generation at the flame front due to the expansion of gases. This unsteady volume generation leads to sound generation by the flame as a distributed monopole source. A sound generation model is developed where ambient pressure fluctuations are generated by this distributed fluctuating heat release source on the flame surface. The flame response framework is used to provide this local heat release source input. This study has been specifically performed for the helical flow disturbance cases to illustrate the effects different modes have on the generated sound. Results show that the effects on global heat release and sound generation are significantly different. Finally, the prediction from the analytical models is compared with experimental data. First, a two-dimensional bluff-body stabilized flame experiment is used to obtain measurements of both the flow and flame position in time. This enables a local flame response comparison since the data are spatially resolved along the flame. Next, a three-dimensional swirl-stabilized lifted flame experiment is considered. The measured flow data is used as input to the G-equation model and the global flame response is predicted. This is then compared with the corresponding value obtained using global CH* chemilumenescence measurements.

Background: Foamy viruses (FVs) have developed a unique budding strategy within the retrovirus family. FV release requires co-expression and a highly specific interaction between capsid (Gag) and glycoprotein (Env), which cannot be complemented by heterologous Env proteins. The interaction domain in FV Env has been mapped in greater detail and resides mainly in the N-terminal tip of the cytoplasmic domain of the Env leader peptide subunit. In contrast, the corresponding domain within Gag is less well defined. Previous investigations suggest that it is located within the N-terminal part of the protein. Results: Here we characterized additional Gag interaction determinants of the prototype FV (PFV) isolate using a combination of particle release, GST pull-down and single cycle infectivity analysis assays. Our results demonstrate that a minimal PFV Gag protein comprising the N-terminal 129 aa was released into the supernatant, whereas proteins lacking this domain failed to do so. Fine mapping of domains within the N-terminus of PFV Gag revealed that the N-terminal 10 aa of PFV Gag were dispensable for viral replication. In contrast, larger deletions or structurally deleterious point mutations in C-terminally adjacent sequences predicted to harbor a helical region abolished particle egress and Gag – Env protein interaction. Pull-down assays, using proteins of mammalian and prokaryotic origin, support the previous hypothesis of a direct interaction of both PFV proteins without requirement for cellular cofactors and suggest a potential direct contact of Env through this N-terminal Gag domain. Furthermore, analysis of point mutants within this domain in context of PFV vector particles indicates additional particle release-independent functions for this structure in viral replication by directly affecting virion infectivity. Conclusions: Thus, our results demonstrate not only a critical function of an N-terminal PFV Gag motif for the essential capsid - glycoprotein interaction required for virus budding but also point out additional functions that affect virion infectivity.

Submitted by Marcio Miazaki null (mmiazaki@gmail.com) on 2017-12-12T15:38:22Z No. of bitstreams: 1 Dissertacao.pdf: 2498214 bytes, checksum: bbe1b8f57d985978dca7748b50feb796 (MD5) / Submitted by Marcio Miazaki null (mmiazaki@gmail.com) on 2017-12-14T11:25:02Z No. of bitstreams: 1 Dissertacao.pdf: 2498214 bytes, checksum: bbe1b8f57d985978dca7748b50feb796 (MD5) / Submitted by Marcio Miazaki null (mmiazaki@gmail.com) on 2017-12-14T13:50:31Z No. of bitstreams: 1 Dissertacao.pdf: 2498214 bytes, checksum: bbe1b8f57d985978dca7748b50feb796 (MD5) / Approved for entry into archive by Cristina Alexandra de Godoy null (cristina@adm.feis.unesp.br) on 2017-12-18T10:25:06Z (GMT) No. of bitstreams: 1 miazaki_m_me_ilha.pdf: 2498214 bytes, checksum: bbe1b8f57d985978dca7748b50feb796 (MD5) / Made available in DSpace on 2017-12-18T10:25:06Z (GMT). No. of bitstreams: 1 miazaki_m_me_ilha.pdf: 2498214 bytes, checksum: bbe1b8f57d985978dca7748b50feb796 (MD5) Previous issue date: 2017-11-30 / Este trabalho apresenta uma proposta de utilização de molas helicoidais de liga com memória de forma presentes em rotores flexíveis para a atenuação dos deslocamentos laterais decorrentes de forças de perturbação, notadamente das forças de desbalanceamento. Num primeiro momento, o trabalho apresenta os principais modelos constitutivos de ligas com memória de forma (LMF), onde o princípio de funcionamento deste tipo de material é demonstrado por meio de simulações numéricas, e os resultados obtidos são confrontados com dados disponíveis na literatura. Discute-se também a questão da utilização de um modelo mais simplificado para representar o comportamento da liga para diferentes carregamentos termomecânicos. Dois modelos termomecânicos são confrontados numericamente visando representar adequadamente o comportamento termomecânico da LMF durante as transformações de fase. O trabalho evolui para a construção do modelo matemático via elementos finitos de rotores flexíveis contendo molas helicoidais de LMF e por fim apresenta um conjunto de simulações numéricas para comprovar a eficiência e a potencialidade do procedimento teórico formulado. O trabalho termina comentando as potencialidades da proposta apresentada, discutindo as facilidades e dificuldades encontradas na sua implementação e apontando para o desenvolvimento de futuros estudos. / This work proposes shape memory alloy helical springs present in flexible rotors for the attenuation of lateral displacements mainly caused by unbalance forces. This work presents some constitutive models of shape memory alloys (SMA). The principle of operation of this type of material is demonstrated by numerical simulations and the results obtained are compared with data available in the literature. The present work investigates the use of a simplified model to represent the behavior of the SMA for different thermomechanical loads. Two thermomechanical models are numerically confronted in order to represent properly the thermomechanical behavior of the SMA during the phase transformations. Based on the finite element model of flexible rotors containing helical springs of SMA, the efficiency and the potentiality of the theoretical procedure are shown through numerical simulations. This work is concluded presenting the potentialities of the design methodology proposed and future developments to be implemented.

Orientador: Gustavo Luiz Chagas Manhães de Abreu / Resumo: Diversos estudos relacionados à atenuação de vibrações utilizando materiais inteligentes vem sendo amplamente explorados no meio acadêmico. Neste âmbito, as Ligas de Memória de Forma (LMF) se destacam por apresentarem dissipação de energia vibratória devido ao seu comportamento histerético promovido pelo efeito pseudoelástico. No presente trabalho, dois sistemas com um e dois graus de liberdade, contendo mola helicoidal de LMF como elemento resiliente, são implementados numericamente para demonstrar a atenuação de vibrações ocasionada pelas transformações de fase presentes no material. Para cada um dos sistemas mecânicos investigados, dois modelos termomecânicos são confrontados numericamente visando a obtenção das características de cada modelo em representar a atenuação de vibrações dos sistemas submetidos à carregamentos termo-mecânicos. O trabalho termina comentando as potencialidades da proposta apresentada, discutindo as facilidades e dificuldades encontradas na sua implementação e apontando para o desenvolvimento de futuros estudos. / Mestre

Orientador: Gustavo Luiz Chagas Manhães de Abreu / Resumo: Este trabalho apresenta uma proposta de utilização de molas helicoidais de liga com memória de forma presentes em rotores flexíveis para a atenuação dos deslocamentos laterais decorrentes de forças de perturbação, notadamente das forças de desbalanceamento. Num primeiro momento, o trabalho apresenta os principais modelos constitutivos de ligas com memória de forma (LMF), onde o princípio de funcionamento deste tipo de material é demonstrado por meio de simulações numéricas, e os resultados obtidos são confrontados com dados disponíveis na literatura. Discute-se também a questão da utilização de um modelo mais simplificado para representar o comportamento da liga para diferentes carregamentos termomecânicos. Dois modelos termomecânicos são confrontados numericamente visando representar adequadamente o comportamento termomecânico da LMF durante as transformações de fase. O trabalho evolui para a construção do modelo matemático via elementos finitos de rotores flexíveis contendo molas helicoidais de LMF e por fim apresenta um conjunto de simulações numéricas para comprovar a eficiência e a potencialidade do procedimento teórico formulado. O trabalho termina comentando as potencialidades da proposta apresentada, discutindo as facilidades e dificuldades encontradas na sua implementação e apontando para o desenvolvimento de futuros estudos. / Abstract: This work proposes shape memory alloy helical springs present in flexible rotors for the attenuation of lateral displacements mainly caused by unbalance forces. This work presents some constitutive models of shape memory alloys (SMA). The principle of operation of this type of material is demonstrated by numerical simulations and the results obtained are compared with data available in the literature. The present work investigates the use of a simplified model to represent the behavior of the SMA for different thermomechanical loads. Two thermomechanical models are numerically confronted in order to represent properly the thermomechanical behavior of the SMA during the phase transformations. Based on the finite element model of flexible rotors containing helical springs of SMA, the efficiency and the potentiality of the theoretical procedure are shown through numerical simulations. This work is concluded presenting the potentialities of the design methodology proposed and future developments to be implemented. / Mestre

At low Reynolds number flows, the effect of inertia becomes negligible and the fluid motion is dominated by the effect of viscous forces. Understanding of the behaviour of low Reynolds number flows underpins the prediction of the motion of microorganisms and particle sedimentation as well as the development of micro-robots that could potentially swim inside the human body to perform targeted drug/cell delivery and non-invasive microsurgery. The work in this thesis focuses on developing an understanding in the mathematical analysis of objects moving at low Reynolds numbers. A boundary element implementation of the Method of regularized Stokeslets (MRS) is applied to analyse the low Reynolds number flow field around an object of simple shape (sphere and cube). It also showed that the results obtained by a boundary element implementation for an unbounded cube, where singularities are presented in the corners of the cube, agrees with more complex solutions methods such as a GBEM and FEM.A methodology for analysing the effect of walls by locating collocation points on the surface of the walls and the object is presented. First at all, this methodology is validated with a boundary element implementation of the method of images for a sphere at different locations. Then, the method is extended when more than one wall is presented. This methodology is applied to predict the velocity filed of a cube moving in a tow tank at low Reynolds numbers for two different cases with a supporting rod similar to an experimental set-up, and without the supporting rod as in the CFD simulations based on the FVM. The results indicate a good match between CFD and the MRS, and an excellent approximation between the MRS and experimental data from PIV measurements. The drag, thrust and torque generated by helices moving at low Reynolds numbers in an unbounded medium is analysed by the resistive force theory, a slender body theory, and a boundary element method of the MRS. The results show that the resistive force theory predict accurately the drag, thrust and torque of moving helices when the resistive force coefficients are calculated from a slender body theory approximation by calculating independently the resistive force coefficients for translation and rotation, because it is observed that the resistive force coefficients depend also of the nature of motion. Moreover, the thrust generated by helices of different pitch angles is analysed calculated by a CFD numerical simulation based on the FVM and a boundary element implementation, an compared with experimental data. The results also show an excellent prediction between the boundary element implementation, the CFD results and the experimental data. Finally, a boundary element implementation of the MRS is applied to predict swimming of a biomimetic swimmer that mimics the motion of E.coli bacteria in an unbounded medium. The results are compared with the propulsive velocity and induced angular velocity measurement by recording the motion of the biomimetic swimmer in a square tank. It is observed that special care needs to be taken when the biomimetic swimmer is modelled inside the tank, as there is an apparent increment in the calculate thrust propulsion which does not represent a real situation of the biometic swimmer which propels by a power supply. However, this increment does not represent the condition of the biomimetic swimmer and a suggested methodology based on the solution from an unbounded case and when the swimmer is moving inside the tank is presented. In addition, the prediction of the free-swimming velocity for the biomimetic swimmer agrees with the results obtained by the MRS when the resistive force coefficients are calculated from a SBT implementation. The results obtained in this work have showed that a boundary element implementation of the MRS produces results comparable with more complex numerical implementations such as GBEM, FEM, FVM, and also an excellent agreement with results obtained from experimentation. Therefore, it is a suitable and easy to apply methodology to analyse the motion of swimmers at low Reynolds numbers, such as the biomimetic swimmer modelled in this work.