no abstract provided by author / Master of Science

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Series of blood samples were taken from Holstein calves before, and at 2, 4, and 6 hours after feeding. The blood plasma levels of tryptophane and methionine were determined by microbiological assay. It was found that the blood plasma levels of tryptophane and methionine varied considerably at different time intervals after feeding milk. One calf was fed 50 grams dl-methionine with its regular feed of milk, and series of blood samples were taken and assayed as in previous work. The methionine level increased to approximately 4 times its initial value by 2 hours after feeding, continued to rise, and at 6 hours after feeding was more than 10 times the original level. At the same time the tryptophane level showed a consistent decline. Determinations for total amino nitrogen were made, using a modification of the Folin-Wu method. The values obtained at different time intervals after feeding ranged from 6.45 to 9.33 mg./100 ml. blood. It was found that feeding methionine increased the blood level for total amino nitrogen, reaching a peak four hours after feeding. A special electrometric titration apparatus was used for making titrations. Approximately 1750 assays, consisting of standards and test samples, were titrated in this experiment. / M.S.

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In 1947, Professor H. S. Miles, Jr. of the Mechanical Engineering Department at the Virginia Polytechnic Institute, presented a thesis (1) on the design of a 65 ton steam jet refrigeration unit. Miles recommended that a small unit be constructed in the Mechanical Laboratory so that the soundness of his design be confirmed. With this in mind, Messrs. A. W. Howard and C. W. Vogelsang (2) built a 5 ton unit in the laboratory in 1948. It is the purpose of the authors of this report to determine why the steam jet refrigerating unit constructed in the Mechanical Laboratory at the Virginia Polytechnic Institute in 1948 did not reach design capacity. / M.S.

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