Content removed due to copyright restriction: Appendix IV. Managing fruit soluble solids with late-season deficit irrigation in drip-irrigated processing tomato production (HortScience 40: 1 857-1861 ). / Improved fertilizer and irrigation management has become increasingly important for tomatoes (Lycopersicon esculentum Mill.) grown for processing. To reduce potential nutrient loss to the environment due to excessive supply, fertilizer recommendations should reflect plant demand determined in an optimal root environment. An aeroponics experiment examined the effect of low and high nutrient supply during vegetative growth, fruit development and fruit ripening. The use of aeroponics in a glasshouse environment allowed control of fertility directly at the root surface. A further experiment applying aeroponics results was established in the field using drip-fertigation. Both studies were conducted at Massey University, Palmerston North. Across experiments, fruit yield was largely determined by vegetative growth in the 6-8 weeks after transplanting; high fruit yields (> 90 Mg ha-1) were associated with improved vegetative growth, and in particular larger leaf area. Mild N deficiency was the principal cause of poor vegetative growth in low nutrient supply treatments. Higher yield resulted from greater fruit number. Reinstating adequate fertility after vegetative growth stopped and fruit number was determined did not increase fruit yield. For maximum fruit yield, plant uptake of N and K was 9.4 and 13.8 g plant-1, respectively (equivalent to approximately 210 and 310 kg ha-1 at a medium planting density). Greatest nutrient uptake occurred during fruit development. Where practical, fertilizer application should be concentrated during fruit growth. Heavy late-season K fertigation did not increase the soluble solids concentration (SSC) of fruit. Although offering considerable flexibility in nutrient fertigation, the use of drip irrigation often results in undesirably low SSC. Late-season irrigation management strategies to increase fruit SSC without excessive yield loss were subsequently investigated in drip-irrigated fields. Two experiments were conducted at the University of California, Davis. Irrigation cutoff prior to fruit ripening reduced fruit set, decreased fruit size, and increased the incidence of fruit rots, making this approach uneconomical. Irrigation cutback to 25-50% of reference evapotranspiration imposed at the onset of fruit ripening (approximately 6 weeks preharvest) was sufficient to improve fruit SSC and maintain Brix yields (Mg Brix solids ha-1) compared to the current grower practice (late cutoff). Irrigation cutbacks imposed during ripening did not cause excessive canopy dieback, nor were fruit culls or rots increased when the crop was harvested at commercial maturity. Fruit colour and pH were not adversely affected by irrigation cutback. Brix monitoring of the earliest ripening fruit (when 30-60 % of the fruit surface shows a colour other than green) can help classify fields as to the severity of irrigation cutback required to reach desirable fruit SSC at harvest. Combined, these techniques offer considerable flexibility in managing fields for improved fruit SSC levels.

This study focuses on modelling community productivity, species abundance and richness, and the impact of climate change and alternative phosphorous fertiliser application strategies on pasture productivity by integration of decision tree and regression modelling approaches with a geographical information system (GIS) in a naturalised hill-pasture ecosystem in the North Island, New Zealand, using data derived from research conducted on hill-pastures over the last several decades. The results indicated that the decision tree models had a high predictive capability and clearly revealed the relative importance of environmental and management factors in influencing community productivity, species abundance and richness. Spring rainfall was the most significant factor influencing annual pasture productivity in the North Island, while hill slope was the most significant factor influencing spring and winter pasture productivity. Annual P fertiliser input and autumn rainfall were the most significant factors influencing summer and autumn pasture productivity, respectively. For species functional group abundance, soil Olsen P was the most significant factor influencing the relative abundance of low fertility tolerance grasses (LFTG) and moss, while soil bulk density, slope and annual P fertiliser input were the most significant factors influencing the relative abundance of legume, high fertility response grasses (HFRG) and flatweeds, respectively. Legume abundance was the most significant factor influencing species richness in the hill-pasture. Species richness increased with an increase in legume abundance and showed a tendency for a hump-shaped response. Grazing animal species also had a significant effect on species richness; pasture grazed by sheep had more species than pasture grazed by cattle. Climate change scenarios of temperature increases of 1-2 °C and rainfall changes of -20% to +20% would have a great impact (-46.2% to +51.9%) on pasture production in the North Island. Pasture in areas with relatively low rainfall had a higher response to increased P fertiliser input than pastures in areas with a relatively high rainfall. In conclusion, the integration of a GIS with decision tree and regression models in this study provided an approach for effective predictive modelling of community productivity, species abundance and richness in the hill-pasture. This modelling approach can also be used as a tool in pasture management such as in assessing the impact of climate change and alternative fertiliser management on pasture production.

This study investigated methods to reduce herbicide application through improved targeting of weeds, thereby also reducing damage to pastures. The focus was to evaluate and improve wiper and spot-spraying application techniques for pasture herbicides as they reduce chemical use by treating just the weed. Wiper application of herbicides was shown to be a useful technique for controlling Californian thistles. In one trial, a stem reduction of over 90% when assessed 10 months post application was achieved with a double pass of clopyralid, metsulfuron and glyphosate when the plants were treated at the post-flowering stage and were vigorously growing. A double pass was superior to a single pass for glyphosate and triclopyr/picloram, but not for clopyralid and metsulfuron. Subsequent trials produced poor results possibly because of the stressed condition of the thistles and their growth stage as well as lack of consistency in wiper output and operator differences. Despite wiper applicators usually being selective, some damage to pastures was observed in the field, and from a series of experiments it was concluded that rain falling soon after wiper application was the likely cause of pasture damage. An innovative and highly sensitive technique using a spectrophotometer was developed to measure herbicide output from wiper applicators. A spectrophotometer could accurately measure clopyralid concentrations as low as 0.02 g active ingredient in a litre of water. The Eliminator and Rotowiper outputs were found to be highly variable while the Weedswiper was more consistent although it applied less herbicide than the other two wipers. Spot spraying experiments confirmed that glyphosate and metsulfuron create bare patches by damaging both grass and clover while clopyralid and triclopyr/picloram only eliminate clover. However, metsulfuron patches stayed bare for much longer while glyphosate ones quickly filled up with weeds and clover. Ingress of clover stolons appeared to be more important than re-establishment from seed in the recovery of patches. The bigger the damaged patch, the higher the likelihood of recolonisation by opportunistic weeds. Bioassay studies found that over-application of clopyralid and triclopyr/picloram provided residual activity up to 18 and 30 weeks, respectively, thereby potentially preventing re-establishment of white clover. The negative effects on clover seedlings from metsulfuron ranged from 3 to 6 weeks for standard and high rates, respectively, with a stimulatory effect on seedlings thereafter for up to 18 weeks. Dose-response curves for the application of metsulfuron and triclopyr/picloram into the centre 5% versus full plant coverage of Scotch thistle and ragwort rosettes showed that application of herbicide to the centre 5% was as effective at the same concentration and greatly reduced the risk of damage to pasture.

Leafy vegetables, lettuce (Lactuca sativa L. cv. Green Oakleaf), pak choi (Brassica sinensis L. cv. Oriental Taisai) and rocket (Eruca sativa L.), were planted in an open field at Lincoln University on January 2005. Plants were harvested at 5 cm cutting height above the growing point with three different harvest start times, early start (ES), mid start (MS) and late start (LS) of harvest respectively. There were three harvests during the growing season with a 4-week interval between harvests. Generally, lettuce produced significantly higher total FW/plant (208 g) than pak choi or rocket (123 and 102 g total FW/plant, respectively). Lettuce also produced significantly higher total FW/plant than pak choi or rocket in each treatment. Lettuce produced significantly more total saleable leaf FW/plant in the ES, MS and LS treatment (137.5, 120.9 and 169.3 g FW/plant), compared with pak choi (52.9, 100 and 92 g FW/plant) or rocket (31.5, 92.2 and 56.4 g FW/plant). Pak choi produced higher total saleable leaf FW/plant than rocket in the ES and LS treatments, but a similar total saleable leaf FW/plant in the MS treatment. The best time to start harvesting was the LS treatment for lettuce, while pak choi and rocket produced better yields in the MS and LS treatments. Results for total DW/plant showed that different harvest start times did not affect the total DW/plant in lettuce. However, pak choi and rocket produced less total DW/plant in the ES treatment compared to the MS or LS treatments. This was due to a faster recovery by lettuce. In addition, lettuce produced significantly higher DW/plant during regrowth at the second and third harvests than pak choi or rocket, even though it was significantly lower at the first harvest. It is likely that after harvesting greater carbohydrate reserves and greater remaining leaf area produced greater regrowth potential in lettuce. These results suggest that lettuce has the most regrowth potential, while rocket has more than pak choi in this reaping and regrowth system. When plants were harvested at the same cutting level, lettuce had the most remaining leaf area of the three species. This remaining leaf area for lettuce could be another reason for faster recovery and more regrowth potential. More reserves and remaining leaf area may also be the reason for more regrowth potential in rocket compared to pak choi. Another important reason for the better performance of lettuce was that temperatures during the growing season were in the range of optimum temperatures (12-21 ºС), except for the regrowth between the second and third harvests in the LS. This relates to higher photosynthetic rates and in turn leads to faster recovery and regrwoth. Lettuce was the most suitable crop in the reaping and regrowth system in this experiment. However, rocket might also be appropriate for this system, particularly if the price is high enough to make it economically viable.

Kiwifruit are of huge economic importance for New Zealand representing 29 percent of total horticultural exports. Fruit size is the biggest determinant of what consumers are willing to pay, and there is also a positive relationship between consumer preference for flavour and percentage dry matter. The two main cultivars exported from New Zealand are Actinidia chinensis ‘Hort 16A’ (gold kiwifruit) and A. deliciosa ‘Hayward’ (green kiwifruit). Under current commercial practice the only product allowed for use on kiwifruit to increase fruit size in New Zealand is Benefit®. Benefit® has been shown to induce different results when applied to A. chinensis and A. deliciosa, whereas synthetic plant growth regulators such as the cytokinin-like substance N-(2- chloro-4-pyridyl)-N’-phenylurea (CPPU) have been found to promote similar increases in fresh weight of fruit in both cultivars. Final fruit size is determined by both cell division and cell enlargement. It was been shown that fresh weight can be increased in both of the major Actinidia cultivars even though their physiology differs. Hormonal control of fruit size in relation to cell division and cell enlargement phases of fruit growth was studied in both A. chinensis and A. deliciosa. CPPU was applied to both cultivars in a growth response experiment where fruit were collected throughout the growing season. The objective of this experiment was to create growth curves, to compare and contrast the effect on A. chinensis and A. deliciosa, and to provide material for hormone analysis. Application of CPPU was found to significantly increase the fresh weight of both A. chinensis and A. deliciosa fruit (46.98 and 31.34 g increases respectively), and alter the ratio of inner and outer pericarps of A. chinensis fruit. CPPU and Benefit® were applied individually and together to both cultivars. It was found that only A. chinesis fruit were affected by the application of Benefit®; fresh weight was increased by 26.38 g, and percentage dry matter was significantly reduced. There was a statistically significant (p < 0.05) interaction between CPPU and Benefit® when applied to A. chinensis. 3,5,6-trichloro-2-pyridyloxyacetic acid (3,5,6-TPA) was applied to A. deliciosa on two application dates at three concentrations and was found to decrease fresh weight of fruit, but significantly increase percentage dry matter regardless of application date or concentration. Lastly CPPU and 1-naphthalene acetic acid (NAA) were applied to A. deliciosa at two application dates and in all combinations. Application date affected the response to both a low concentration of CPPU and NAA. A synergistic interaction was observed when CPPU was applied early plus NAA late (CPPU early (4.53 g increase) plus NAA late (13.29 g) < CPPU early plus NAA late (33.85 g). Finally endogenous hormone content was studied. Methods were developed and tested for the simultaneous analysis of both indole-3-acetic acid (IAA) and cytokinins. Freeze dried fruit were purified using Waters Sep-pak® cartridges and Oasis® columns then IAA was quantified by high pressure liquid chromatography. Preliminary results indicate a correlation between application of CPPU and endogenous IAA, high concentrations of IAA correlated well with periods of rapid fruit growth particularly for CPPU treated fruit.

Content removed due to copyright restrictions: Burggraaf, V.T., Kemp, P.D., Thom, E.R., Waghorn,G.C., Woodfield, D.R. & Woodward, S.L. (2004) Performance of dairy cows grazing white clover selected for increased floral condensed tannin. Preliminary report from experiments presented in Chapter 4 published in the 2004 Proceedings of the New Zealand Grassland Association. / Legumes containing 20 to 40 g of condensed tannin (CT) per kg of dry matter (DM) can improve dairy cow milk production by reducing ruminal protein degradation to ammonia and preventing bloat. White clover (Triflium repens L.) produces CT in its flower heads. High tannin (HT) white clover, bred for increased flowering and increased floral CT concentration, was evaluated under dairy grazing in Hamilton, New Zealand. Its performance in monoculture was compared to that of Grasslands Huia white clover over two years, and five short-term grazing experiments determined its effects on Friesian dairy cows. Huia and HT had similar floral CT concentrations, ranging from 15 to 77 g/kg DM over two flowering seasons. HT clover had higher flower densities than Huia until the second summer after sowing, resulting in higher clover (leaf plus flower) CT concentrations. Clover CT peaked at 12.1 g/kg DM for HT and 5.7 g/kg DM for Huia. HT swards had lower stolon growing point densities than Huia swards and annual DM yields averaged 10.0 and 11.0 t DM/ha for the respective clovers. The ingress of non-sown white clover genotypes reduced treatment differences in the last 10 months of the experiment. Mild bloat occurred in cows grazing both clovers. Cows grazing HT white clover had rumen ammonia concentrations 5 to 26% lower than that of cows grazing Huia, indicating less proteolysis in the rumen of HT cows, but there were no consistent effects on rumen soluble protein or volatile fatty acids (VFA). Differences between treatments in dietary CT concentrations were too small to affect milk production or composition. Minced mixtures of 0, 25, 50, 75 or 100% of DM as white clover flower with the remainder as white clover leaf, were incubated in vitro and rumen metabolite concentrations determined at 0, 2 ,4, 8, 12 and 24 hours. Polyethylene glycol was added to one of the 50% flower treatments to inactivate CT. Clover flowers had less soluble protein than leaves at 0 hours, and increasing the percentage of flowers from 0 to 100% reduced the net conversion of plant-N to ammonia-N from 29 to 12%. The contribution of CT to these effects was small. Increasing percentages of clover flowers did not significantly affect total VFA production but increased acetate to propionate (A:P) ratios. White clover CT decreased A:P ratios. In another in vitro experiment perennial ryegrass leaf (Lolium perenne L.) was incubated either alone or with white clover flowers or birdsfoot trefoil (Lotus corniculatus L.). Clover flowers were more effective at reducing proteolysis than birdsfoot trefoil, due largely to less release of soluble protein, but birdsfoot trefoil treatments had the lowest A:P ratios. In conclusion, HT clover had higher forage CT concentrations than Huia because of increased flowering. Increased flowering reduced the agronomic performance of HT and lowered rumen ammonia concentrations, but did not increase milk production or prevent bloat. White clover flowers reduced rumen proteolysis in vitro, but this was mainly a result of their low protein concentration. White clover CT and birdsfoot trefoil forage benefited the molar percentages of VFA, but increasing the proportion of clover flowers did not. Further increases in white clover CT concentrations may benefit ruminant performance, but this should not be implemented through increased flowering.

Content removed due to copyright restrictions: Burggraaf, V.T., Kemp, P.D., Thom, E.R., Waghorn,G.C., Woodfield, D.R. & Woodward, S.L. (2004) Performance of dairy cows grazing white clover selected for increased floral condensed tannin. Preliminary report from experiments presented in Chapter 4 published in the 2004 Proceedings of the New Zealand Grassland Association. / Legumes containing 20 to 40 g of condensed tannin (CT) per kg of dry matter (DM) can improve dairy cow milk production by reducing ruminal protein degradation to ammonia and preventing bloat. White clover (Triflium repens L.) produces CT in its flower heads. High tannin (HT) white clover, bred for increased flowering and increased floral CT concentration, was evaluated under dairy grazing in Hamilton, New Zealand. Its performance in monoculture was compared to that of Grasslands Huia white clover over two years, and five short-term grazing experiments determined its effects on Friesian dairy cows. Huia and HT had similar floral CT concentrations, ranging from 15 to 77 g/kg DM over two flowering seasons. HT clover had higher flower densities than Huia until the second summer after sowing, resulting in higher clover (leaf plus flower) CT concentrations. Clover CT peaked at 12.1 g/kg DM for HT and 5.7 g/kg DM for Huia. HT swards had lower stolon growing point densities than Huia swards and annual DM yields averaged 10.0 and 11.0 t DM/ha for the respective clovers. The ingress of non-sown white clover genotypes reduced treatment differences in the last 10 months of the experiment. Mild bloat occurred in cows grazing both clovers. Cows grazing HT white clover had rumen ammonia concentrations 5 to 26% lower than that of cows grazing Huia, indicating less proteolysis in the rumen of HT cows, but there were no consistent effects on rumen soluble protein or volatile fatty acids (VFA). Differences between treatments in dietary CT concentrations were too small to affect milk production or composition. Minced mixtures of 0, 25, 50, 75 or 100% of DM as white clover flower with the remainder as white clover leaf, were incubated in vitro and rumen metabolite concentrations determined at 0, 2 ,4, 8, 12 and 24 hours. Polyethylene glycol was added to one of the 50% flower treatments to inactivate CT. Clover flowers had less soluble protein than leaves at 0 hours, and increasing the percentage of flowers from 0 to 100% reduced the net conversion of plant-N to ammonia-N from 29 to 12%. The contribution of CT to these effects was small. Increasing percentages of clover flowers did not significantly affect total VFA production but increased acetate to propionate (A:P) ratios. White clover CT decreased A:P ratios. In another in vitro experiment perennial ryegrass leaf (Lolium perenne L.) was incubated either alone or with white clover flowers or birdsfoot trefoil (Lotus corniculatus L.). Clover flowers were more effective at reducing proteolysis than birdsfoot trefoil, due largely to less release of soluble protein, but birdsfoot trefoil treatments had the lowest A:P ratios. In conclusion, HT clover had higher forage CT concentrations than Huia because of increased flowering. Increased flowering reduced the agronomic performance of HT and lowered rumen ammonia concentrations, but did not increase milk production or prevent bloat. White clover flowers reduced rumen proteolysis in vitro, but this was mainly a result of their low protein concentration. White clover CT and birdsfoot trefoil forage benefited the molar percentages of VFA, but increasing the proportion of clover flowers did not. Further increases in white clover CT concentrations may benefit ruminant performance, but this should not be implemented through increased flowering.

The contamination of process pea (Pisum sativum L.) crops by the immature fruit of black nightshade (Solanum nigrum L.) and hairy nightshade (S. physalifolium Rusby var. nitidibaccatum (Bitter.) Edmonds) causes income losses to pea farmers in Canterbury, New Zealand. This thesis investigates the questions of whether seed dormancy, germination requirements, plant growth, reproductive phenology, or fruit growth of either nightshade species reveal specific management practices that could reduce the contamination of process peas by the fruit of these two weeds. The seed dormancy status of these weeds indicated that both species are capable of germinating to high levels (> 90%) throughout the pea sowing season when tested at an optimum germination temperature of 20/30 °C (16/8 h). However, light was required at this temperature regime to obtain maximum germination of S. nigrum. The levels of germination in the dark at 20/30 °C and at 5/20 °C, and in light at 5/20 °C, and day to 50 % germination analyses indicated that this species cycled from nondormancy to conditional dormancy throughout the period of investigation (July to December 2002). For S. physalifolium, light was not a germination requirement, and dormancy inhibited germination at 5/20 °C early in the pea sowing season (July and August). However, by October, 100% of the population was non-dormant at this test temperature. Two field trials showed that dark cultivation did not reduce the germination of either species. Growth trials with S. nigrum and S. physalifolium indicated that S. physalifolium, in a non-competitive environment, accumulated dry matter at a faster rate than S. nigrum. However, when the two species were grown with peas there was no difference in dry matter accumulation. Investigation of the flowering phenology and fruit growth of both species showed that S. physalifolium flowered (509 °Cd, base temperature (Tb) 6 °C) approximately 120 °Cd prior to S. nigrum (633 °Cd). The fruit growth rate of S. nigrum (0.62 mm/d) was significantly faster than the growth rate of S. physalifolium (0.36 mm/d). Because of the earlier flowering of S. physalifolium it was estimated that for seedlings of both species emerging on the same date that S. physalifolium could produce a fruit with a maximum diameter of 3 mm ~ 60 °Cd before S. nigrum. Overlaps in flowering between peas and nightshade were examined in four pea cultivars, of varying time to maturity, sown on six dates. Solanum physalifolium had the potential to contaminate more pea crops than S. nigrum. In particular, late sown peas were more prone to nightshade contamination, especially late sowings using mid to long duration pea cultivars (777-839 °Cd, Tb 4.5 °C). This comparison was supported by factory data, which indicated that contamination of crops sown in October and November was more common than in crops sown in August and September. Also, cultivars sown in the later two months had an ~ 100 °Cd greater maturity value than cultivars sown in August and September. Nightshade flowering and pea maturity comparisons indicated that the use of the thermal time values for the flowering of S. nigrum and S. physalifolium can be used to calculate the necessary weed free period required from pea sowing in order to prevent the flowering of these species. The earlier flowering of S. physalifolium indicates that this species is more likely to contaminate pea crops than is S. nigrum. Therefore, extra attention may be required where this species is present in process pea crops. The prevention of the flowering of both species, by the maintenance of the appropriate weed free period following pea sowing or crop emergence, was identified as potentially, the most useful means of reducing nightshade contamination in peas.

The association between individual plant performance and seed yield variability within and between field pea crops was investigated. In 1988/89 six F8 genotypes with morphologically distinct characteristics were selected from a yield evaluation trial. Analysis of the individual plant performance within these crops indicated an association between low seed yields and the location and dispersion of plant harvest index (PHI) and plant weight (PWT) distributions. The analyses also showed there was a strong linear relationship between the seed weight (SWT) and PWT of the individual plants within each crop, and that the smallest plants tended to have the lowest PHI values. A series of 20 simulations was used to formalize the relationships between SWT, PWT and PHI values within a crop into a principal axis model (PAM). The PAM was based on a principal axis which represented the linear relationship between SWT and PWT, and an ellipse which represented the scatter of data points around this line. When the principal axis passed through the origin, the PHI of a plant was independent of its PWT and the mean PHI was equal to the gradient of the axis. However, when the principal axis had a negative intercept then the PHI was dependent on PWT and a MPW was calculated. In 1989/90 four genotypes were sown at five plant populations, ranging from 9 to 400 plants m⁻². Significant seed and biological yield differences were detected among genotypes at 225 and 400 plants m⁻². The plasticity of yield components was highlighted, with significant genotype by environment interactions detected for each yield component. No relationship was found between results for yield components from spaced plants and those found at higher plant populations. The two highest yielding genotypes (CLU and SLU) showed either greater stability or higher genotypic means for PHI than genotypes CVN and SVU. Despite significant skewness and kurtosis in the SWT, PWT, and PHI distributions from the crops in this experiment, the assumptions of the PAM held. The lower seed yield and increased variability in PHI values for genotype CVN were explained by its higher MPW and the positioning of the ellipse closer to the PWT axis intercept than in other genotypes. For genotype SVU, the lower seed yield and mean PHI values were explained by a lower slope for the principal axis. Both low yielding genotypes were originally classified as having vigorous seedling growth and this characteristic may be detrimental to crop yields. A method for selection of field pea genotypes based on the PAM is proposed. This method enables the identification of weak competitors as single plants, which may have an advantage over vigorous plants when grown in a crop situation.

The general goal of this research was to understand the agronomic and physiological changes of a lucerne crop in distinct physical radiation environments and to verify the potential of lucerne to grow under shaded conditions. To achieve this, the research was conducted in four main steps: (i) firstly, experimental data collection in the field using two artificial shade materials (shade cloth and wooden slats) under inigated and non-irrigated conditions; (ii) a second experiment with data collection in a typical temperate dryland agroforestry area under non-irrigated conditions; (iii) generation of a light interception sub-model suitable for shaded crops and (iv) a linkage between the light interception sub-model and a canopy photosynthesis model for agroforestry use. In experiments 1 and 2, lucerne crop was exposed to 6 different light regimes: full sunlight (FS), shade cloth (FS+CL), wooden slats (FS+SL), trees (T), trees+cloth (T +CL) and trees+slats (T+SL). The FS+SL structure produced a physical radiation environment (radiation transmission, radiation periodicity and spectral composition) that was similar to that observed in the agroforestry site (f). The mean annual photosynthetic photon flux density (PPFD) was 41 % under the FS+CL, 44% under FS+SL and 48% under T compared with FS in clear sky conditions. Plants were exposed to an intermittent (sun/shade) regime under both FS+SL and T, whereas under FS+CL the shaded light regime was continuous. The red to far-red (RIFR) ratio measured during the shade period under the slats was 0.74 and under the trees was 0.64. However, R/FR ratio increased to 1.26 and 1.23 during the illuminated period under FS+SL and T, respectively, and these were equivalent to the ratio of 1.28 observed under the FS+CL and 1.31 in FS. The radiation use efficiency (RUE) of shoots increased under the 5 shaded treatments compared with full sunlight. The pattern of radiation interception was unchanged by radiation flux, periodicity and spectral composition and all treatments had a mean extinction coefficient of 0.82. However, the magnitude of the decrease in canopy growth was less than those in PPFD transmissivity. The mean lucerne annual dry matter (DM) yield was 17.5 t ha⁻¹ in FS and 10 t ha⁻¹ under the FS+CL, FS+SL and T regimes. This declined to 3.4 t DM ha⁻¹ under T+CL (22% PPFD transmissvity) and 4.1 t DM ha⁻¹ under T+SL (23% transmissivity). A similar pattern of response was observed for leaf net photosynthesis (Pn) rates under the shade treatments compared with full sun. In addition, spectral changes observed under the trees and slats affected plant motphology by increasing the number of long stems, stem height and internode length compared with full sunlight. Thus, there were two main explanations for the increase in RUE under shade compared with full sun: (i) preferential partition of assimilates to shoot rather than root growth and/or (ii) leaves under shade were still operating at an efficient part of the photosynthetic light curve. The changes proposed for the canopy Pn model were appropriate to simulate the radiation environment of an agroforestry system. However, the model underestimated DM yields under the continuous and intermittent shade regimes. These were considered to be mainly associated with plant factors, such as overestimation in maintenance respiration and partitioning between shoots and roots in shade and the intermittency light effect on leaf Pn rates. Further investigation in these topics must be addressed to accurately predict crop yield in agroforestry areas. Overall, the lucerne crop responded typically as a sun-adapted plant under shade. It was concluded that lucerne yield potential to grow under intermediate shade was superior to most of C3 pastures previously promoted in the literature.