Studies on knowledge, attitudes and practices among healthcare workers involved in HIV and AIDS care have often revealed the lack of knowledge about HIV and AIDS. Nurses‘ knowledge may compromise the quality of care and attitudes towards patients living with HIV/AIDS. Special nursing knowledge and skills have been suggested as a prerequisite for taking care of patients with HIV. The purpose of this study was to assess nurses‘ knowledge, attitudes and practices towards patients with HIV and AIDS in Kumasi, Ghana. A quantitative cross-sectional study was conducted among 247 nurses at five selected health facilities in Kumasi. Data was collected by means of a structured self-administered questionnaire and analysed using SPSS version 23.0. Results were presented using charts and tables. Knowledge of HIV and AIDS was satisfactory but some of the nurses still hold erroneous beliefs and misconception about HIV transmission. A majority demonstrated favourable attitudes. Nurses had fears of contracting the virus, which resulted in the display of negative attitudes by some. Their practice of universal precautions was satisfactory; however, there was evidence of noncompliance among some of them. More studies should be conducted throughout the country to further assess nurses‘ knowledge, attitude and practices towards HIV and AIDS / Health Studies / M.A. (Nursing Science)