Diabetes is a rapidly increasing health problem. In a global perspective,approximately 415 million people suffered from diabetes in 2015 and this number ispredicted to increase to 640 million by 2040. To tackle this pandemic there is a needfor better analytical tools by which we can increase our understanding of the disease.One discipline that has already provided much needed insight to diabetes etiology isoptical molecular imaging. Using various forms of light it is possible to create animage of the analysed sample that can provide information about molecularmechanistic aspects of the disease and to follow spatial and temporal dynamics. The overall aim of this thesis is to improve and adapt existing andnovel optical imaging approaches for their specific use in diabetes research. Hereby,we have focused on three techniques: (I) Optical projection tomography (OPT),which can be described as the optical equivalent of x-ray computed tomography(CT), and two vibrational microspectroscopic (VMS) techniques, which records theunique vibrational signatures of molecules building up the sample: (II) Fouriertransforminfrared vibrational microspectroscopy (FT-IR) and (III) Ramanvibrational microspectroscopy (Raman). The computational tools and hardware applications presented here generallyimprove OPT data quality, processing speed, sample size and channel capacity.Jointly, these developments enable OPT as a routine tool in diabetes research,facilitating aspects of e.g. pancreatic β-cell generation, proliferation,reprogramming, destruction and preservation to be studied throughout the pancreaticvolume and in large cohorts of experimental animals. Further, a novel application ofmultivariate analysis of VMS data derived from pancreatic tissues is introduced.This approach enables detection of novel biochemical alterations in the pancreasduring diabetes disease progression and can be used to confirm previously reportedbiochemical alterations, but at an earlier stage. Finally, our studies indicate thatRaman imaging is applicable to in vivo studies of grafted islets of Langerhans,allowing for longitudinal studies of pancreatic islet biochemistry.viIn summary, presented here are new and improved methods by which opticalimaging techniques can be utilised to study 3D-spatial, quantitative andmolecular/biochemical alterations of the normal and diseased pancreas.

The evolution of depth‐perception visualization technologies, emerging format standardization work and research within the field of multi‐view 3D video and imagery addresses the need for flexible 3D video visualization. The wide variety of available 3D‐display types and visualization techniques for multi‐view video, as well as the high throughput requirements for high definition video, addresses the need for a real‐time 3D video playback solution that takes advantage of hardware accelerated graphics, while providing a high degree of flexibility through format configuration and cross‐platform interoperability. A modular component based software solution based on FFmpeg for video demultiplexing and video decoding is proposed,using OpenGL and GLUT for hardware accelerated graphics and POSIX threads for increased CPU utilization. The solution has been verified to have sufficient throughput in order to display 1080p video at the native video frame rate on the experimental system, which is considered as a standard high‐end desktop PC only using commercial hardware. In order to evaluate the performance of the proposed solution a number of throughput evaluation metrics have been introduced measuring average frame rate as a function of: video bit rate, video resolution and number of views. The results obtained have indicated that the GPU constitutes the primary bottleneck in a multi‐view lenticular rendering system and that multi‐view rendering performance is degraded as the number of views is increased. This is a result of the current GPU square matrix texture cache architectures, resulting in texture lookup access times according to random memory access patterns when the number of views is high. The proposed solution has been identified in order to provide low CPU efficiency, i.e. low CPU hardware utilization and it is recommended to increase performance by investigating the gains of scalable multithreading techniques. It is also recommended to investigate the gains of introducing video frame buffering in video memory or to move more calculations to the CPU in order to increase GPU performance.

Tato práce popisuje konstrukci skenovacího systému pro tvorbu trojrozměrných modelů. Kombinace laserového scanneru a robotického manipulátoru tvoří velice flexibilní zařízení schopné snímat jak velké, tak malé a detailní objekty. Zařízení nachází uplatnění v mnoha aplikacích, zejména v lékařství, kde přináší řadu nesporných výhod proti stávajícím systémům. Práce popisuje mechanickou konstrukci zařízení, funkční principy a jeho kalibrační proceduru. Součástí práce je i software pro vizualizaci naměřených dat a jejich zpracování do podoby modelů se stínovaným povrchem. Výsledkem práce je funkční zařízení a rozsáhlý obslužný software.

I dagens samhälle är digitalisering en självklarhet genom olika former av applikationer vilka samspelar med människan. Det ställer höga krav på både användare och utvecklare, att de ska vara anpassningsbara. Inom fastighetsbranschen är visualisering ett väl etablerat verktyg för att kommunicera information, och en del av digitaliseringen sker genom mjukvaror som 3D-applikationer. Det är givet att dessa visualiseringsapplikationer måste förhålla sig till ett antal punkter som vilken information som ska presenteras, hur det ska förmedlas samt vilken nivå av abstraktion att bistå. På senare tid har begreppet användarbarhet blivit väldigt populärt där det grundläggande är att designa med människan i centrum. Det är en strategi för interaktiv utveckling där fokus är användarna och definierar vad folk har för kunskap, vad de arbetar med, gör och känner. Vid utveckling av dessa mjukvaror är det viktigt att just fokusera på individen för att en produkt eller tjänst ska bli så god som möjligt, vilket underlättar acklimatiseringen för slutanvändarna. Denna undersökning har fokuserat på användarupplevelsen av design som koncept samt existerande teorier vilket behandlar principer och element i 3D. Målgruppen har varit utvecklare av 3D-applikationer där undersökningen gjorts på fastighetsspekulanter, deras åsikter har legat som grund för viktiga funktioner inom en mjukvara. Datainsamlingen har skett enligt kvalitativa intervjuer, litteraturstudie och en benchmarkundersökning. Undersökningen har visat att respondenterna i det stora hela bekräftar tidigare forskning, med mindre bortfall. I studien har det framkommit ytterligare delar som är viktiga både från undersökningen med respondenterna och genom benchmarkingen.

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