Complex Exocyst consists of eight proteins and it is known as a Sec6/8. Its composition is evolutionarily highly conserved amongst all the species. This complex is involved in vesicle trafficking as a part of attaching mechanism to a specific place on the plasma membrane. EXO70 subunit has been found in 23 copies in Arabidopsis thaliana genome. In this study we have been examine paraloges EXO70H7 and EXO70H8. There have been suggestions that these genes are important in development of roots according to the previous studies. We have not been able to identify any significant phenotype within the mutant plants in these genes. There has been studied other mutant appearance during the stress experiments. Most of these experiments did not identify any divergence. Only experiments with germination during stress conditions revealed significantly worse germination of exo70H7 mutant seeds on the medium containing sorbitol. This suggests that mutant seeds have a worse protection against osmotic stress. Significantly worse germination of exo70H8 seeds on the medium with excess NaCl indicates that these seeds incriminate to higher absorption of sodium ions. Analysis of the cell localization of GFP constructs brought knowledge of appearance EXO70H7 and EXO70H8 proteins. EXO70H7 locates in the cytoplasm and in...

>Magister Scientiae - MSc / In agriculture crop productivity is greatly affected by stresses such as salinity, drought, temperature and honnonal changes of crop plants and responses to these stresses. Studies have shown that a natriuretic peptide based regulatory system responsible for water and ion homeostasis in animals has a hctcrologous equivalent in plants. Plant natriuretic peptide immunoreactants (irPNPs) have been reported to be involved in K+, Na+ and er ions fluxes in plants. Previously, an Arabidopsis thaliana transcript (AtPNP-A) encoding an irPNP (AtPNP-A) has been identified and isolated (Ludidi et al., 2002). The AtPNP-A a novel protein and part of its physiological role is presented here.

京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第20213号 / 理博第4298号 / 新制||理||1617(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)講師 嶋田 知生, 教授 長谷 あきら, 教授 鹿内 利治 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DFAM

No description available.

No description available.

No description available.

No description available.

No description available.

No description available.

No description available.