Diss. (sammanfattning) Stockholm : Karol. inst., 2005. / Härtill 5 uppsatser.

The predicted acceptor impurity nature of carbon in hexagonal GaN grown by molecular-beam epitaxy (MBE) is revisited spectroscopically in the energy range between 1.6 and 3.5 eV. Photoluminescence (PL) spectra from ultra-pure GaN and material doped with carbon at a level of 1·18 cm⁻³ differ significantly in the energy range between 3.0 and 3.3 eV depending on the Ga/N stoichiometry during MBE growth. Acceptor-like features formerly attributed to carbon, beryllium or magnesium incorporation are found for both, undoped and carbon-doped GaN. The intensity of these features depends on the Ga/N stoichiometry during growth. While for Ga-lean surface regions, exhibiting multiple 10 nm deep pits, the observed PL features are found to be less intense compared to Ga-rich surface regions, the situation reverses for carbon-doped material. For all samples, the intensity of the yellow luminescence band around 2.2 eV is weak. The results point at crystal defects and the unintentionally present oxygen as the origin of the spectroscopic features traditionally attributed to carbon in GaN.

Thesis (M. Arch.)--Massachusetts Institute of Technology, Dept. of Architecture, 1988. / Includes bibliographical references (leaf 139). / The particular subject of this thesis is Housing for the Elderly. The intention is to propose an architecture which springs from an alternative attitude towards housing the elderly ... an attitude which purports that multiplicity and exchange are the elements crucial to one's well-being; in contrast to the current paradigm within the world of planning for the elderly ... one of segregation, classification and singularity. With a little license, I call this a research and design thesis, as it has been difficult to define. As a 'research' paper, it entails the use of a particular method of selecting, understanding, and (finally) using a range of references in order to distill from them some underlying principles of form. Yet unlike a true research thesis, it is loosely structured and left open-ended ... intentionally so! The 'design' work provides the testing ground for the principles gleaned from those references. It also allows me the opportunity to exercise myself in synthesizing and arranging architecture. Yet as a design thesis, the work is only the start of an architecture process ... far from being complete. In essence, the thesis is an endeavour in knowing a little more about the 'dancing' between referencing and designing. / by Julia A. Campbell. / M.Arch.

This research project aims to develop a novel A3 thinking approach to support knowledge driven design that aids the generation of decision making within a Lean Product and Process Development (LeanPPD) environment. This research comprises the development of a new A3 template as a technique of problem solving in product design, the adoption of a reflection practice structured in a new A3 template for knowledge capture and sharing, and the generation of the process of using the A3 thinking approach for effective implementation. Providing useful knowledge as a design reference to generate decision making at the initial stages of product development in product design helps the designers to prevent recurrence of the same problem, eliminate design mistakes and enhance design decision. In order to achieve a novel A3 thinking approach, a research methodology consisting of four phases was developed. The first phase synthesises the A3 best practice through literature and documentation reviews. The gap analysis and results from the reviews have identified several problem-solving approaches and learning cycles that have to be considered in the research. The second phase is to evaluate the approaches and their impacts and applications in product design. In order to complete this, several research methods are selected and performed (e.g. focus group and semistructured interview) within the collaborative companies. The third phase is to develop the A3 thinking approach by utilising the LAMDA learning cycle, developing a new A3 template or so-called A3LAMDA, adopting the reflection practice and generating the process of using the new A3 thinking approach. Finally, the validation of the new A3 thinking approach through industrial case studies and expert judgements have been performed. This approach has been implemented in the automotive sector and was applied to four industrial case studies and six A3LAMDA reports were collected. As a result of the findings of this research, the utilisation of the A3 thinking approach aided the generation of knowledge driven design in product design by integrating the knowledge management capabilities; knowledge creation, capture and sharing.

This research thesis presents the development of a novel Lean Knowledge Life Cycle (LeanKLC) framework to support the transformation into a Lean Product Development (LeanPD) knowledge environment. The LeanKLC framework introduces a baseline model to understand the three dimensions of knowledge management in product development as well as its contextualisation with acclaimed LeanPD process models. The LeanKLC framework comprises 23 tasks, each accomplished in one of the seven key stages, these being: knowledge identification, previous knowledge capture, knowledge representation, knowledge sharing, knowledge integration, knowledge use and provision and dynamic knowledge capture. The rigorous research methodology employed to develop the LeanKLC framework entailed extensive data collection starting with a literature review to highlight the gap in the current body of knowledge. Additionally, industrial field research provides empirical evidence on the current industrial perspectives and challenges in managing product development knowledge. This research was part of a European FP7 project entitled Lean Product and Process Development (LeanPPD), which provided the opportunity to involve industrial collaborators in action research to support practical aspects during the LeanKLC framework development. The synthesis with the current LeanPD paradigm is accomplished by demonstrating the LeanKLC stages in two distinct streams related to the development of A3 thinking for problem solving and the development of trade-off curves to facilitate set based design at the conceptual stage. The novel LeanKLC is validated in two case studies providing the industry with detailed insights on real product development applications. In particular this research highlights that the LeanPD knowledge environment is a wide subject area that has not yet been thoroughly understood and that industry engagement in empirical research is vital in order to realise any form of LeanPD transformation.

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Made available in DSpace on 2016-06-02T20:34:23Z (GMT). No. of bitstreams: 1 3353.pdf: 9748915 bytes, checksum: a7360f11a05ffa6f61ca6a629af312cd (MD5) Previous issue date: 2010-09-03 / Financiadora de Estudos e Projetos / This PhD thesis covered was devoted to the preparation of nitrogenated intermediates by multicomponent reactions (3-MCR) with potential application in the bioactive compound synthesis. The developed methodology consisted on the coupling reaction of an organometallic compound, formed by an alkyne and a metal salt or complex, and a nitrogenated electrophile like as imines or iminium salts. In the first part of this work we reported the A3 coupling reaction of aldehydes, 4-piperidone hydrochloride monohydrate and alkynols giving the corresponding hydroxy alkynyl piperidin-4-ones, which are important synthetic building blocks. In the second part of the work was developed a coupling reaction using benzylamine as primary amine source in order to prepare amino alkynols with the benzil group. These compounds were employed, as synthetic intermediates, in the synthesis of bioactive five, six and seven membered alkaloids. / Neste trabalho está descrita a preparação de intermediários sintéticos nitrogenados, através de reações multicomponentes (3-RMC), com potencial aplicação na síntese de compostos bioativos. A reação estudada promove o acoplamento de um composto organometálico, gerado a partir de um alquino e um sal metálico ou sal complexo, e um composto nitrogenado eletrofílico como iminas e sais de imínio, gerados in situ. A primeira parte do trabalho apresenta a reação de acoplamento A3 entre aldeídos, o sal hidroclorídrico da 4-piperidona e alquinóis levando a formação de hidroxi alquinil piperidin-4-onas, que são importantes blocos sintéticos. Na segunda parte do trabalho foi desenvolvida uma reação de acoplamento utilizando benzilamina como fonte de amina, visando à obtenção de benzilamino alquinóis. Estes compostos foram empregados como intermediários na síntese de alcaloides bioativos de cinco, seis e sete membros.

In recent decades the interest of wheat with non-standard discoloration of caryopsis has increased, because of its positive impacts on health. The pigments anthocyanins, which are included in the protective layer of grain, have antioxidant effects. A group of genotypes from Agrotest fyto Ltd. Kroměříž was chosen which consisted of eighteen samples of wheat with non-standard discoloration of caryopsis. Fourteen samples were blue (aleuron), tree yellow (endosperm) and one sample had a red (pericarp). By these samples PCR-based analyses were performed to detect the Waxy gene zero allele, the Glu-A3 glutenin locus and the hardness of grain by puroindoline genes. These parameters are significant indicators of wheat quality. By all genotypes the presence of allele Pina-D1a/b was detected and by a few the allele Pinb-D1a/c/d. These detected alleles, which are included in the analyzed wheat, should be sorted to the hardness wheats because they are suitable for baking purposes. The identified material (V1 104 15), a so-called waxy genotype, was positive in the presence of all three zero alleles in the Waxy gene. Similar lines were used in Asia for the preparation of pastas. At the locus Glu-A3, the Glu-A3d allele was detected in all tested genotypes. Along with this, Glu-A3f was founded in some genotypes. These alleles have a positive effect on dough quality, especially on ductility. The results will be used for further breeding work.

Soybeans with large seed size, uniformity, clear hilum, and high 11S/7S ratio are favored for soymilk and tofu making. In order to find ideal soybean lines for food making, sixty-eight soybean lines, which were selected from thousands of accessions in the USDA-Soybean Germplasm Collection, were planted in three successive seasons. Eight lines were identified from twenty-two lines harvested in 2014 (Stoneville, MS) to be suitable for tofu making as judged by chemical composition and sensory quality of tofu. The results provided important food quality information to the growers, breeders and tofu industries for their selection of soybean to improve food quality. In the filled tofu making and texture analysis study, the correlation between A3 subunit percentage and tofu firmness was significant (N=22. r = 0.77, P < 0.001). The result proved that the percentage of A3 subunits could be an indicator for predicting the firmness of tofu.

Objective: The aim was to determine if in vitro ischaemic preincubation can precondition cardiomyocytes and if the responses to adenosine receptor antagonists are similar to those previously determined during "metabolic" preconditioning with glucose deprivation or adenosine agonists. Methods: Isolated rabbit cardiomyocytes were preconditioned with 10 min of ischaemic preincubation, followed by a 30 min postincubation before the final sustained ischaemic period. The protein kinase C inhibitor calphostin C or the adenosine receptor antagonists 8-sulphophenyltheophylline (SPT), BW 1433U, and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) were added either during the preincubation or into the final ischaemic pellet. Adenosine deaminase (10 U · ml-1) was added during ischaemic preincubation. Rates of contracture and extent of injury were determined by sequential sampling and assessment of trypan blue permeability following 85 mOsM swelling. Results: Myocytes were preconditioned by a 10 min in vitro ischaemic preincubation. Preincubation with 100 μM SPT or with adenosine deaminase, or addition of 200 nM calphostin C into the final ischaemic pellet did not alter rates of rigor contracture but nearly abolished protection. A significant degree of protection was maintained following ischaemic preincubation with the highly selective adenosine A1 receptor blocker DPCPX (10 μM), while the antagonist BW 1433U (1 μM) severely limited protection. SPT and BW 1433U added only into the final ischaemic pellet of preconditioned cells significantly blocked protection, while protection was maintained in the presence of DPCPX. Conclusions: Ischaemic preconditioning of cardiomyocytes is blocked by adenosine receptor antagonists known to bind to A3 receptors but not by DPCPX which has high affinity for A1 receptors, but little affinity for A3 receptors. Maintenance of protection during the final ischaemic phase has a similar receptor specificity. Blockade of protein kinase C activity abolishes protection. Ischaemic and metabolic preconditioning in vitro appear to occur through similar pathways.