Global ETD Search

151	Models for enzymatic nucleotide cleavage Dalby, Kevin Nicholas January 1992 (has links) No description available. 572 Phosphates; Nucleic acids
152	Growth studies on mycobacterium BCG Moore, David Franklyn January 1981 (has links) No description available. 579 Amino acids; Vaccines
153	An investigation into mechanisms underlying the regulation of nitric oxide synthesis in the rat cerebellum Toms, Nicholas Jeremy January 1994 (has links) No description available. 615.1 Excitatory amino acids
154	The synthesis and testing of novel cholic acid based stationary phases Tierney, Juliann Jude January 2002 (has links) No description available. 547 Bile acids
155	Synthesis and pharmacological characterisation of novel agonists for AMPA and kainate receptors based on the natural product willardiine Troop, Helen Marie January 2000 (has links) No description available. 615.1 Amino acids; Medicinal
156	Derivatisation and characterisation of type II polyketide synthase acyl carrier proteins Byrom, Katherine Jane January 1998 (has links) No description available. 572 Fatty acids
157	Synthesis of #beta#-carboxyaspartic acid derivatives and studies on 3-methoxycarbonylmethylene-3,4,5,6-tetrahydro- 2H-1,4-oxazine-2-one derivatives Laughton, Peter January 1997 (has links) No description available. 547 Amino acids; Oxazinone
158	Characterisation of a human stearoyl CoA desaturase gene (SCD2) Dempsey, Kate E. January 2001 (has links) No description available. 572.8 Enzyme; Fatty acids
159	Synthesis of methionine and conformationally restricted tryptophan analogues and their incorporation into neuropeptides Nichols, Paul David January 1994 (has links) No description available. 615.1 Peptides; Amino-acids
160	POLYAMINE-MEDIATED DEGRADATION OF ORNITHINE DECARBOXYLASE IN CHINESE HAMSTER OVARY CELLS. GLASS, JAMES RUSSELL. January 1987 (has links) The objective of this research was to identify specific mechanisms involved in the regulation of ornithine decarboxylase, the first enzyme in the polyamine biosynthetic pathway. Immunochemical techniques were used to study post-translational modifications of the ODC protein in relation to activity alterations. Initial experimentation showed that Chinese hamster cells maintained in a defined medium express an ODC protein stable to intracellular degradation. Treatment of these cells with exogenous ornithine or polyamines resulted in a rapid loss of enzyme activity, without detectable changes in the enzyme specific activity. The loss of enzyme activity was a result of accelerated ODC degradation, as determined by immunoprecipitation of pre-labeled protein. In addition, spermidine, but not ornithine, totally inhibited new ODC synthesis. The mechanism of accelerated ODC degradation was investigated and found to occur by an apparent novel mechanism. Degradation of ODC was both ubiquitin-independent and non-lysosomal, and there was also no detectable accumulation of a modified form of ODC protein. In addition, it was found that a component of protein synthesis is required for this process, as inhibitors (cycloheximide, emetine, puromycin) blocked polyamine-accelerated degradation. ODC cDNA was used to synthesize both ODC specific mRNA and protein using in vitro synthesis. These systems may allow the generation of sufficient quantities of material which can be used to recreate in vitro the specific components involved in polyamine inhibition of ODC synthesis and the protease(s) responsible for degradation. The major finding of this work is the direct demonstration that ODC is a stable intracellular protein in the absence of putrescine and spermidine depleted cells (Chapter 2). In addition, that degradation occurs by a novel mechanism, with a requirement for some component of protein synthesis (Chapter 3). Finally, these studies describe the in vitro production of ODC protein and mRNA, which should facilitate further studies of polyamine regulation of ODC degradation and synthesis (Chapter 4). Decarboxylases. Amino acids. Polyamines.

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