A three-column ion-exchange system was designed, fabricated and computer-automated to accommodate a novel 'elution looping' process developed by Dr. Tim Durance (U.B.C. Department of Food Science) during his doctoral studies on the recovery of lysozyme and avidin. This processing technique enhances the simultaneous recovery of these two pharmaceutically important proteins from chicken egg albumen. The processing system prototype was sized to handle throughput rates between approximately five and 300 liters per day of albumen to facilitate both laboratory and small commercial scale work. Very efficient use is made of the ion-exchange resin due to a two-column cascaded feed arrangement. The processing control software was designed to provide flexibility and ease of operation in setting up new and existing method files, allowing for the selection of any column or group of columns to use and providing a 'staged-shutdown' approach toward handling columns fouled with congealed albumen during unattended operation. This approach attempts to maximize the productivity of the system even when one or two of the columns has become fouled with congealed albumen. / Applied Science, Faculty of / Chemical and Biological Engineering, Department of / Graduate

Meta-analysis refers to the quantitative synthesis of information across different studies. Since outcomes from different studies are likely to be reported in different units, study-level results are typically transformed to the same scale before quantitative integration. Typically, this leads to the accumulation and combination of effect sizes. To date, most social scientists have synthesized, or meta-analyzed, zero-order statistics like a correlation. Synthesizing partial effect sizes is an alternative which allows a meta-analysis to account for the influence of nuisance variables when estimating the association between two variables. This dissertation proposes that logistic regression coefficients from different studies, which are a type of partial effect size, can be meta-analyzed. Logistic regression models how a set of covariates relates to a binary dependent variable. Given a key independent variable (IV) of interest, which we can call the focal IV or Xf, the slope estimate (βf) in a logistic regression measures the impact of Xf on Y on the logit (log-odds) scale, while controlling for other variables. Four assumptions justify the possibility of comparing and possibly combing logistic slopes across studies: (1) Y must be on the same scale, (2) Xf must be on the same scale, (3) all effect sizes are logistic regression slopes adjusted for the same covariates, and (4) model specifications are identical. In practice, the third assumption is particularly challenging as different studies inevitably include different sets of control variables. Three simulation studies are implemented to understand how synthesizing a logistic regression slope on the logit scale is affected by several factors. Across these three simulation studies, the following meta-analytic variables are tested: (1) the size of the partial effect size (βf), (2) Study-level sample size (k), (3) Within-study sample size (N), (4) the degree of between-study variance, (5) a continuous vs. a binary focal predictor, (6) the level of collinearity between Xf and other covariates included in primary studies, (7) the magnitude of non-focal variable slopes, (8) different covariate sets used in primary-level studies, and (9) meta-analytical method. Simulation performance is based on how the bias and mean-squared error (MSE) are affected by each of these simulation parameters. Overall, results suggest that when the four assumptions introduced above are satisfied, meta-analyzing logistic regression slopes is remarkably accurate as the summary effect resulting from the standard random-effects meta-analytic model leads to small levels of bias and MSE under a variety of conditions. When the assumptions are broken (and particularly the third assumption of identical covariate sets), the pooled slope estimator can have large degrees of bias. The bias is a function of within-study sample size, between-study sample size, distribution of the focal IV (i.e., continuous vs. categorical variable), multicollinearity, the magnitude of non-focal variable slope parameters, diversity in covariate sets, and choice of meta-analytical methods. The MSE is a function of study-level sample size, within-study sample size, distribution of the focal IV (i.e., continuous vs. categorical variable), multicollinearity, the magnitude of non-focal variable slope parameters, diversity in covariate sets, and choice of meta-analytical methods. A complex four-way interaction is discovered between collinearity, the magnitude of non-focal variable slope parameters, diversity in covariate sets, and choice of meta-analytical methods. An applied example focusing on estimating the effects of albumin on mortality is also presented to complement the simulation results.

<p>U okviru disertacije ispitivan je uticaj sorte i mikroklimatskih uslova tokom dve proizvodne godine p&scaron;enice na sadržaj i strukturu albumina; izvr&scaron;ena je karakterizacija albumina pomoću Lab&ndash;on&ndash; a&ndash;Chip elektroforeze; određena je aktivnost proteolitičkih i amilolitičkih enzima, sadržaj slobodnih sulfhidrilnih i slobodnih amino grupa; ispitana su reolo&scaron;ka svojstva testa primenom uobičajenih metoda za procenu tehnolo&scaron;kog kvaliteta bra&scaron;na i izvedena je karakterizacija gotovog proizvoda-hleba. Jedan deo istraživanja se odnosio na period posležetvenog dozrevanja p&scaron;enice i stabilizacije bra&scaron;na.<br />Obe ispitivane godine odlikovao je toplotni stres s tim &scaron;to su u 2012. godini maksimalne<br />temperature prelazile 35 &deg;C i broj tropskih dana je bio izrazito veći u odnosu na 2011., &scaron;to je uslovilo promene u sastavu i kvalitetu proteina i skroba u zrnu p&scaron;enice. Sadržaj ukupnih albumina uzoraka p&scaron;eničnog bra&scaron;na iz 2012. bio je značajno manji u odnosu na 2011. proizvodnu godinu. Rezultati određivanja proteolitičke i amilolitičke aktivnosti uzoraka p&scaron;eničnog bra&scaron;na iz dve proizvodne godine su pokazali da su za 2012. proizvodnu godinu karakteristične znatno niže vrednosti ovih pokazatelja u odnosu na 2011. proizvodnu godinu i da je enzimska aktivnost pre svega sortna karakteristika.<br />Sadržaj slobodnih sulfhidrilnih grupa uzoraka p&scaron;eničnog bra&scaron;na iz 2012. proizvodne godine bio je značajno veći dok je sadržaj disulfidnih veza bio manji u odnosu na 2011. Sadržaj slobodnih amino grupa se razlikovao između uzoraka iz dve proizvodne godine, kako između različitih sorti, tako i u pogledu sva tri primenjena tretmana inkubacije glutena. Značajne razlike između vrednosti slobodnih amino grupa izmerenih nakon inkubacije gluten na 37 &deg;C, ukazuju na različitu proteolitičku aktivnost ispitivanih uzoraka p&scaron;eničnih sorti. U poređenju sa uzorcima iz 2011. proizvodne godine, uzorci iz 2012. su imali znatno niže vrednosti specifične zapremine hleba.<br />Dodatkom dvostruke količine sopstvenih albumina odabranom setu uzoraka dobijeni<br />su rezultati, koji ukazuju da su uzorci bra&scaron;na iz 2011. imali manjak amilolitičkih enzima, a uzorci iz 2012. manjak proteolitičkih enzima za postizanje optimalnog tehnolo&scaron;kog kvaliteta.</p> / <p>In this dissertation, the influence of variety and microclimatic conditions that prevailed during the two production years on the content and structure of wheat albumins were investigated; characterization of albumins was performed by Lab-on-a-Chip capillary electrophoresis; the proteolytic and &alpha;- amylolytic activity, as well as the content of free sulfhydryl and free amino groups were also determined; the rheological properties of dough were estimated using conventional methods for the assessment of flour technological quality and characterization of the final product- bread was performed. One part of the research covered the period of postharvest wheat and flour maturation. Heat stress was characteristic of both production years; however, in 2012, maximum temperatures exceeded 35 &deg;C and the number of days with maximum temperatures above 30 &deg;C was markedly higher than in the 2011 production year. These conditions have caused the changes in the composition and quality of protein and starch in wheat kernels. The albumin content of wheat flour samples from 2012 was significantly lower compared to 2011 production year.&nbsp;Results of proteolytic and amilolytic activities of wheat flour samples from two production years, showed that the values of these indicators were significantly lower for 2012 in relation to the 2011 production year, and that the enzyme activity is primarily varietal characteristic. The content of free sulfhydryl groups of wheat flour samples from the 2012 production year was significantly higher while the content of disulfide bonds was lower than in 2011. The content of free amino groups differed between samples from two production years. Differences in the amount of free amino content were evident between the varieties and between different treatments of gluten incubation. Significant differences between the values of the free amino groups measured after gluten incubation at 37 &deg;C, indicate a different proteolytic activity of tested wheat flour samples. In comparison with the samples from the 2011 production year, samples from 2012 had significantly lower values of specific bread volume. The addition of double amount of its own albumins to the selected samples indicated&nbsp;that the flour samples from 2011 had a deficit of amylolytic enzymes and samples from 2012 had a deficit of proteolytic enzymes for achieving optimal technological quality.</p>

Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 242-263).

Diss. (sammanfattning) Linköping : Linköpings universitet, 2008. / Härtill 5 uppsatser.

Diss. (sammanfattning) Umeå : Umeå universitet, 2009. / Härtill 5 uppsatser.

Thesis (M.S.)--Florida State University, 2006. / Advisor: Timothy S. Moerland, Florida State University, College of Arts and Sciences, Dept. of Biological Science. Title and description from dissertation home page (viewed Sept. 15, 2006). Document formatted into pages; contains x, 33 pages. Includes bibliographical references.

ABO blood type has been shown to be associated with both cardiovascular risk and toxicity‐preventing activity (TxPA) stress response in elderly males. Twenty middle‐aged, healthy males, 14 blood type A and six blood type O, were involved in this project. Volunteers completed a battery of psychological assessments, then gave blood and had several psychophysiological measures taken prior to, during and after two stressors. The stressors consisted of mental arithmetic tasks plus audiotapes of combat sounds and a baby crying. The anger‐out and hard‐driving scores of blood type O subjects were significantly higher than the blood type A means. TxPA decreased significantly as a function of stress and some suggestive blood type effects of TxPA were found. Plasma protein, microhematocrit, plasma cortisol, finger temperature, skin conductance, blood pressure and two facial electromyograph (EMG) variables were also significantly affected by stressors but not by the blood type factor. No significant differences of any kind were found for total cholesterol, high‐density lipoprotein or pulse variables. The importance of age and other individual subject characteristics was discussed.

La fotoquímica supramolecular es una herramienta muy utilizada para controlar la selectividad, la reactividad y el avance de las reacciones químicas. Con ella se aprovecha la estructura tridimensional del anfitrión para canalizar la conformación de los estados excitados de los sustratos que intervienen en la reacción. En la literatura se encuentran numerosos casos de fotorreacciones catalizadas por supramoléculas abióticas, pero los ejemplos con biomoléculas son escasos. En este contexto, el objetivo de la presente tesis ha sido estudiar reacciones fotoquímicas clásicas en el seno de biomoléculas ya que éstas pueden presentar ventajas respecto a las supramoléculas abióticas. Se han escogido las albúminas séricas como anfitriones bióticos, en base a dos propiedades claves: (i) son proteínas transportadoras que actúan como vehículo de una amplia variedad de sustancias; (ii) poseen dos sitios de unión diferenciados: el sitio I y el sitio II. La reorganización de foto-Fries fue la primera reacción abordada. Para ello se diseñaron varios sustratos que interaccionaban en los sitios de unión I y II de distintas albúminas. Distintos estudios cinéticos demostraron que los rendimientos de formación de los fotoproductos dependían del sitio de interacción y de la clase de albúmina utilizada. Se seleccionaron también derivados de avobenzona (AB) sustituídos en la posición ? de los carbonilos. Se demostró que éstos sólo existen bajo su forma ?-dicetónica, la cual puede desencadenar procesos de fototoxicidad. Se estudió mediante fotólisis de destello láser el efecto de la interacción de los tres derivados con HSA y los resultados mostraron el efecto protector de ésta frente al ataque por oxígeno y la auto-desactivación y reflejaron el entorno confinado proporcionado por la albúmina. Además, se investigó la fragmentación de Norrish tipo II de uno de los derivados, que resultó ser más lenta en presencia de albúmina. Por último, se consideró la fotoelectrociclación [6?] de la N- / Marín Melchor, M. (2013). Proteïnes com a microreactors en fotoquímica supramolecular [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/27204

Introdução: A reanimação fluídica da criança queimada é um desafio devido à intolerância à insuficiente ou excessiva oferta de líquidos. Há dúvidas em relação à utilização de solução coloide na ressuscitação volêmica e também quanto ao melhor momento a ser administrada. O momento ideal para a administração de albumina permanece em foco de debate, se deveria ser utilizada como estratégia de resgate, quando o volume de cristaloide infundido se torna excessivo, ou rotineiramente, como intervenção primária em pacientes com queimaduras extensas. Objetivos: Avaliar e comparar quanto a evolução clínica de crianças com lesões térmicas que receberam abordagem de infusão precoce (entre 8 e 12 horas do acidente) de solução coloide natural versus crianças que receberam abordagem de infusão tardia (após 24 horas do acidente) da mesma solução para reanimação na fase aguda. Metodologia: Ensaio Clínico Randomizado Controlado, realizado no Centro de Tratamento de Queimados do Hospital Universitário de Londrina. Foram estudadas 46 crianças (1 a 12 anos), apresentando entre 15% e 45% de Superfície Corporal Queimada, admitidas até a 12a hora após o acidente. Intervenção: Para a ressuscitação hídrica dos pacientes, foi utilizada solução cristaloide baseada na Fórmula de Parkland modificada, ajustada de acordo com o débito urinário. O Grupo Intervenção (23 pacientes) foi randomizado para receber solução de albumina entre 8 e 12 horas do acidente, e o Grupo Controle (23 pacientes) recebeu a mesma solução após 24 horas do acidente. Resultados: Houve possibilidade de redução de infusão de solução cristaloide durante o período de ressuscitação dos pacientes. O grupo Intervenção recebeu um volume de solução cristaloide com uma mediana de -31,99% (P=0,025) no 1º dia, -19,37% (P=0,002) no 2º dia e -45,3% (P=0,002) no 3º dia de ressuscitação. Não foram observadas diferenças significantes entre os grupos em relação à diurese. A incidência acumulada de fluid creep na população estudada foi de 30,43% (n=14). O RR para o desenvolvimento do fluid creep no Grupo Intervenção foi de 0,0769 (IC 95% 0,0109 a 0,5407). A mediana do tempo de internação dos pacientes do Grupo Controle foi de 18 (15-21) dias e a do Grupo Intervenção foi de 14 (10-17) dias, P=0,004. Conclusões: A infusão precoce de solução de albumina em crianças com queimaduras entre 15% e 45% de superfície corporal reduziu a necessidade de infusão de solução cristaloide no período de ressuscitação. Foram identificados significativamente menos casos de fluid creep e observado menor tempo de internação entre pacientes que receberam albumina precocemente / Introduction: The fluidic resuscitation of burned children is a challenge due to intolerance to insufficient or excessive supply of liquids. There are questions regarding the use of colloids in fluid resuscitation solution as well as the timing of the administration. The ideal time for the administration of albumin, in other words whether it should be used as a rescue strategy when the volume of crystalloid infused becomes excessive, or routinely, as primary intervention for patients with extensive burns, remains in the focus of discussion. Objectives: Evaluate the clinical outcomes of children with burn injuries who have received early infusion treatment (between 8 and 12 hours after the accident) with natural colloids solution compared to the children who have received late infusion treatment (24 hours after the accident) with the same solution, in order to resuscitate them in the acute phase. Methods: Randomized Controlled Trial carried out at the Burn Treatment Center, State University of Londrina. Forty-six children (1 to 12 year olds) who had between 15% and 45% total body surface area and were admitted up to 12 hours after the accident were studied. Intervention: For the fluid resuscitation of patients, a crystalloid solution based on modified Parkland Formula was adjusted according to urine output. The Intervention Group (n= 23) were randomized to receive albumin solution between 8 and 12 hours after the accident and the Control Group (n= 23) received the same solution later than 24 hours after the burn injury. Results: During the resuscitation of patients, it was possible to reduce the infusion of crystalloid solution. The Intervention Group required a volume of crystalloid solution with a median of -31.99% (P = 0.025) on day 1, - 19.37% (P = 0.002) on day 2 and -45.3% (P = 0.002) on day 3 of resuscitation. No significant differences were observed in the groups in relation to diuresis. The cumulative incidence of fluid creep in the population studied was 30.43% (n=14). The RR for the development of fluid creep in the Intervention Group was 0.0769 (IC 95% 0.0109 to 0.5407). The patients in the Control Group spent an average time of 18 (15-21) days in hospital, while the patients in the Intervention Group spent 14 (10-17) days, P=0,004. Conclusions: The early infusion of albumin solution in children with 15% to 45% total body surface area reduced the need of crystalloid infused during the resuscitation period. Significantly lower cases of fluid creep were identified and lower length of stay was observed among patients who were treated earlier with albumin