Made available in DSpace on 2014-12-17T14:03:40Z (GMT). No. of bitstreams: 1 LuizaSEPW_DISSERT.pdf: 2731256 bytes, checksum: a154afda557a95d39d943b76672810db (MD5) Previous issue date: 2012-12-21 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study examines the physical and chemical composition and the pharmacological effects of brown seaweed FRF 0.8 Lobophora variegata. Fractionation of the crude extract was done with the concentration of 0.8 volumes of acetone, obtaining the FRF 0.8. The physicochemical characterization showed that it was a fucana sulfated. Anti-inflammatory activity was assessed by paw edema model by the high rates of inhibition of the edema and the best results were in the fourth hour after induction (100 ? 1.4% at the dose of 75 mg / kg) and by the strong inhibitory activity of the enzyme myeloperoxidase (91.45% at the dose of 25 mg / kg). The hepataprote??o was demonstrated by measurements of enzymatic and metabolic parameters indicative of liver damage, such as bilirubin (reduction in 68.81%, 70.68% and 68.21% for bilirubin total, direct and indirect, respectively at a dose of 75 mg / kg), ALT, AST and γ-GT (decrease of 76.93%, 44.58% and 50% respectively at a dose of 75 mg / kg) by analysis of histological slides of liver tissue, confirming that hepatoprotective effect the polymers of carbohydrates, showing a reduction in tissue damage caused by CCl4 and the inhibition of the enzyme complex of cytochrome P 450 (increasing sleep time in 54.6% and reducing the latency time in 71.43%). The effectiveness of the FRF 0.8 angiogenesis was examined in chorioallantoic membrane (CAM) of fertilized eggs, with the density of capillaries evaluated and scored, showing an effect proangig?nico at all concentrations tested FRF (10 mg- 1000 mg). The FRF showed antioxidant activity on free radicals (by inhibiting Superoxide Radical in 55.62 ? 2.10%, Lipid Peroxidation in 100.15 ? 0.01%, Hydroxyl Radical in 41.84 ? 0.001% and 71.47 Peroxide in ? 2.69% at concentration of 0.62 mg / mL). The anticoagulant activity was observed with prolongation of activated partial thromboplastin time (aPTT) at 50 mg (> 240 s), showing that its action occurs in the intrinsic pathway of the coagulation cascade. Thus, our results indicate that these sulfated polysaccharides are an important pharmacological target / Este estudo analisa a composi??o f?sico-qu?mica e os efeitos farmacol?gicos da FRF 0,8 da alga marrom Lobophora variegata. O fracionamento do extrato bruto foi feito com a concentra??o de 0,8 volumes de acetona, obtendo a FRF 0,8. A caracteriza??o f?sico-qu?mica mostrou que se tratava de uma fucana sulfatada. Foi verificada a atividade antinflamat?ria pelo modelo de edema de pata, atrav?s das altas taxas de inibi??o do edema e os melhores resultados foram na quarta hora ap?s a indu??o (100 ? 1,4% com a dose de 75 mg/kg) e pela forte atividade inibidora da enzima mieloperoxidase (91,45% com a dose de 25 mg/kg). A hepataprote??o foi demonstrada pelas dosagens de par?metros metab?licos e enzim?ticos indicativos de dano hep?tico, como bilirrubina (redu??o em 68,81%, 70,68% e 68,21% para bilirrubinas total, direta e indireta, respectivamente na dose de 75 mg/kg), ALT , AST e γ-GT (diminui??o em 76,93%, 44,58% e 50%, respectivamente na dose de 75 mg/kg), pela an?lise das l?minas histol?gicas do tecido hep?tico, que confirmam esse efeito hepatoprotetor dos pol?meros de carboidrato, mostrando uma redu??o no dano tecidual causado por CCl4, e pela inibi??o do complexo enzim?tico do citocromo P 450 (aumentando o tempo de sono em 54,6% e reduzindo o tempo de lat?ncia em 71,43%). A efic?cia sobre a angiogenese da FRF 0,8 foi examinada na membrana corioalant?ica (CAM) de ovos fertilizados, com a densidade dos capilares avaliadas e pontuadas, mostrando um efeito proangig?nico em todas as concentra??es de FRF testadas (10 μg-1000 μg). A FRF apresentou a??o antioxidante sobre radicais livres (inibindo o Radical Super?xido em 55,62?2,10%, Peroxida??o Lip?dica em 100,15?0,01%, Radical Hidroxila em 41,84?0,001% e Per?xido em 71,47?2,69%, todos na concentra??o de 0,62 mg/mL). A atividade anticoagulante foi verificada com o prolongamento do tempo de tromboplastina parcial ativada (aPTT) a 50 μg (>240 s), mostrando que sua a??o ocorre na via intr?nseca da cascata de coagula??o. Sendo assim, nossos resultados indicam que estes polissacar?deos sulfatados constituem um importante alvo farmacol?gico

Made available in DSpace on 2014-12-17T15:32:16Z (GMT). No. of bitstreams: 1 RuthLFX.pdf: 2397744 bytes, checksum: 8d25a9f2aa705aa972d7a1d4de2b1b29 (MD5) Previous issue date: 2008-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The purpose of this study was to assess the immunohistochemical expression of CD105 and FvW antibodies in the angiogenesis of oral epidermoid carcinoma (OEC), correlating it with the TNM clinical staging system, seeking a better understanding of its biological behavior and use as an indicator of prognosis.The sample consisted of 30 epidermoid carcinoma (EC) cases, 10 of the floor of the mouth, 10 of the retromolar region and 10 of the tongue, in addition to 10 cases of pyogenic granuloma, which made up the control group. The results showed that mean microvessel counts (MVC) were correspondingly higher in the pyogenic granuloma group (CD105 = 57.26 vessels and FvW = 39.64) than in the EC group (CD105 = 10.09 and FvW = 12.20) and that the differences were statistically significant between the groups for each of the angiogenic biomarkers (p = 0.002 for CD105 and p< 0.001 for FvW). CD105 had better positivity in the pyogenic granuloma group (mean = 57.26 vessels) and for EC, FvW had the highest expression (mean = 12.20 vessels). With respect to EC, the most affected age group was between 51 and 70 years (n = 14; 46.7%), with a representative MVC for both markers. No statistically significant difference was found between the sexes for any of the markers (p = 0.967 for CD105 and p = 0.744 for FvW). Mean CD105 levels were much higher in patients with stage T3 and T4 (17.13) and lower in those with stage N+ (6.36). Mean FvW levels were higher in the patients with stage T1 and T2 (12.23) and lower in patients with T3 and T4 (12.10), but without a statistically significant difference. In regard to anatomic location, a statistically significant difference was observed between FvW sites, with a statistically significant difference between floor of the mouth cases and those located in the retromolar region (p =0.013). Therefore, this study suggests that CD105 expression in OEC angiogenesis, in contrast to other types of malignant neoplasias, may not be correlated with prognosis and tumor aggressiveness, whereas FvW was a more effective antibody for staining this lesion / Nesta pesquisa buscou-se avaliar a express?o imunoistoqu?mica dos anticorpos CD105 e FvW na angiog?nese do Carcinoma Epiderm?ide Oral (CEO), correlacionando-o com o estadiamento cl?nico pelo sistema TNM, visando uma melhor compreens?o do seu comportamento biol?gico e utiliza??o como indicador de progn?stico. A amostra foi composta por 30 casos de CE, sendo 10 de assoalho bucal, 10 da regi?o retromolar e 10 de l?ngua, al?m de 10 casos de granuloma piog?nico, integrantes do grupo controle. Os resultados desta pesquisa mostraram que as m?dias da MVC foram correspondentemente mais elevadas no grupo do granuloma piog?nico (CD105 = 57,26 vasos e FvW = 39,64) do que no grupo do CE (CD105 = 10,09 e FvW = 12,20) e as diferen?as se revelaram estatisticamente significantes entre os grupos para cada um dos biomarcadores angiog?nicos (p=0,002 para o CD105 e p<0,001 para o FvW ). O CD105 se mostrou com melhor positividade no granuloma piog?nico (m?dia = 57,26 vasos) e, para o CE, o FvW foi o que apresentou maior marca??o (m?dia = 12,20 vasos). Com rela??o ao CE, a faixa et?ria mais acometida foi entre 51 e 70 anos (n=14; 46,7%), apresentando uma MVC representativa para ambos os marcadores. N?o se comprovou diferen?a estatisticamente significante entre os sexos para nenhum dos marcadores (p=0,967 para o CD105 e p=0,744 para o FvW). A m?dia do CD105 foi bem mais elevada entre os pacientes com estadiamento T3 e T4 (17,13) e menos elevada entre os pacientes com estadiamento N+ (6,36). Quando se avaliou o FvW, a m?dia foi mais elevada no grupo dos pacientes com T1 e T2 (12,23), sendo mais baixa nos pacientes com T3 e T4 (12,10), por?m sem diferen?a estatisticamente significante. Em rela??o ? localiza??o anat?mica, comprovou-se diferen?a estatisticamente significante entre as localiza??es assoalho bucal e retromolar (p=0,013) para o marcador FvW. Portanto, este estudo sugere que a marca??o do CD105 na angiog?nese do CEO, ao contr?rio de outros tipos de neoplasias malignas, pode n?o estar correlacionada com o progn?stico e agressividade do tumor, enquanto que o FvW se mostrou um anticorpo mais efetivo na marca??o desta les?o

Made available in DSpace on 2014-12-17T15:32:16Z (GMT). No. of bitstreams: 1 MarceloGV.pdf: 2007167 bytes, checksum: 5ee7e9c9bd56fd72fecb1874fb64a80e (MD5) Previous issue date: 2008-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Angiogenesis, a fundamental mechanism in tumor development, is used for differential diagnosis and prognosis purposes in various neoplasias of the head and neck. This study proposes to assess angiogenic activity using immunohistochemical expression by anti-CD105 and anti-CD34 antibodies in 20 cases of hemangiomas and 20 cases of oral pyogenic granulomas, in addition to determining the usefulness of these markers as one of the differential diagnosis resources for these two oral lesions. The results showed no statistically significant difference between microvascular count (MVC) means determined by anti-CD105 (p = 0.803) and anti-CD34 (p = 0.279) antibodies. The mean number of vessels obtained by MVC in the oral hemangiomas immunostained by anti-CD105 and anti-CD34 was 18.75 and 59.72, respectively, whereas in the oral pyogenic granulomas, the mean number was 20.22 and 48.09 respectively. It was also shown that CD34 was more effective than CD105 in identifying blood vessels. However, it must be pointed out that the anti-CD105 antibody seems to be more related to vascular neoformation. Overall, this assay reinforces the role of angiogenic factors in the etiopathogenesis of hemangiomas and oral pyogenic granulomas, but the results showed that angiogenesis quantification cannot be used as a differential diagnosis parameter between the two lesions analyzed / A angiog?nese ? um mecanismo fundamental para o desenvolvimento tumoral, sendo utilizada para fins de diagn?stico diferencial e determina??o de progn?stico em v?rias neoplasias de cabe?a e pesco?o. Este trabalho se prop?s avaliar a atividade angiog?nica atrav?s da express?o imuno-histoqu?mica dos anticorpos anti-CD105 e anti-CD34 em 20 casos de hemangiomas e 20 casos de granulomas piog?nicos orais, al?m de averiguar a utilidade destes marcadores como um dos recursos de diagn?stico diferencial para estas duas les?es orais. Os resultados deste experimento demonstraram que n?o houve diferen?a estatisticamente significativa entre as m?dias de contagem microvascular (MVC) determinadas pelos os anticorpos anti-CD105 (p=0,803) e anti-CD34 (p=0,279). O n?mero m?dio dos vasos obtidos pela MVC nos esp?cimes de hemangiomas orais imunomarcados pelo anti-CD105 e anti-CD34 foram respectivamente 18,75 e 59,72, enquanto nos granulomas piog?nicos orais, o n?mero m?dio dos vasos obtidos pela MVC pelo anti-CD105 e anti-CD34 foram respectivamente 20,22 e 48,09. Foi verificado, tamb?m, que o CD34 foi mais efetivo na identifica??o de vasos sang??neos quando comparado com o CD105. Entretanto, faz-se necess?rio destacar, que o anticorpo anti-CD105 parece estar mais relacionado com a neoforma??o vascular. Em linhas gerais, este ensaio refor?a a participa??o dos fatores angiog?nicos na etiopatog?nese dos hemangiomas e granulomas piog?nicos orais, por?m os resultados mostraram que a quantifica??o da angiog?nese n?o pode ser utilizada como par?metro de diagn?stico diferencial entre as duas les?es analisadas