Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-01-14T17:01:28Z No. of bitstreams: 1 TES_LIANA_ANTUNES_PARCIAL.pdf: 6357992 bytes, checksum: 7d2cbe20d0b95a3897695c3c5ed15c28 (MD5) / Made available in DSpace on 2016-01-14T17:01:28Z (GMT). No. of bitstreams: 1 TES_LIANA_ANTUNES_PARCIAL.pdf: 6357992 bytes, checksum: 7d2cbe20d0b95a3897695c3c5ed15c28 (MD5) Previous issue date: 2015-08-31 / Background: Invariant natural killer T (iNKT) cells play complex functions in the immune system, releasing both Th1 and Th2 cytokines. The role of iNKT cells in human asthma is still controversy and never described in severe therapy asthmatic children (STRA). Objective: To analyse iNKT frequency in peripheral blood of children with severe therapy-resistant asthma (STRA), comparing with children with milder asthma and healthy controls. Methods: This is a case-control study where blood was collected from asthmatic children (n=136) (milder and STRA) and healthy controls (n=40). Peripheral blood mononuclear cells (PBMC) were isolated, stained with anti-CD3 and anti-iNKT (V?24J?18), and analysed through flow cytometry. Atopic status was defined by measuring specific IgE in serum. Airway inflammation was assessed through induced sputum. Results: Children with asthma (n = 136) presented an increased frequency of CD3+iNKT+ cells (mean 2.31% SD 4.41), compared to healthy controls (n = 40) (mean 0.48% SD 0.79) (p = 0.02). Mild-asthmatic children (n = 99) showed an increased frequency of iNKT cells (2.65% SD 4.91; p = 0.002) compared to STRA (n = 11) (2.05% SD 1.62). STRA children have shown an increase frequency of iNKT cells (2.05% SD 1.62) than controls (0.48% SD 0.79; p = 0.002). The number of iNKT cells was not different between atopic (n = 127) and non-atopic (n = 45) children. In addition, iNKT cells were not associated with any inflammatory pattern of induced sputum studied classified as eosinophilic (n = 18), neutrophilic (n = 23), mixed (n = 31) (eosinophilic and neutrophilic) and paucigranulocytic (n = 5). However, iNKT cell numbers were not associated with any airway inflammatory pattern (p=0.09) Conclusion and Clinical Relevance: Our data suggested a role to iNKT in paediatric asthma since it increased in asthmatic children; however these cells are not associated with atopic status or with the more severe spectrum of the disease. / Introdu??o: C?lulas invariante de c?lulas NK (iNKT) desempenham fun??es complexas no sistema imune, liberando citocinas do tipo Th1 e Th2. O papel das c?lulas invariante de c?lulas NK (iNKT) na asma em humanos ainda ? controverso e nunca foi descrito em crian?as com asma grave resistente ? terapia (AGRT). Objetivo: Analisar a frequ?ncia de c?lulas iNKT no sangue perif?rico de crian?as com asma grave resistente ? terapia (AGRT), comparando com crian?as com asma leve e controles saud?veis. M?todos: Este ? um estudo de caso-controle onde foi coletado sangue de crian?as asm?ticas com asma leve e AGRT (n=136) com idade entre 11.01?1.69 e controles saud?veis (n=40) com idade entre 11.21?1.16 anos. A fra??o mononuclear do sangue perif?rico (PBMC) foi isolada, marcada com anti-CD3+ e anti-iNKT (V?24J?18) e analisada no cit?metro de fluxo. A atopia foi definida atrav?s da medi??o dos n?veis plasm?ticos de IgE. A inflama??o das vias a?reas foi avaliada atrav?s da expectora??o induzida. Resultados: As crian?as com asma (n = 136) apresentaram um aumento da frequ?ncia de c?lulas iNKT+CD3+ (m?dia: 2.31%?4.41), quando comparadas com os controles saud?veis (n=40) (m?dia: 0.48%?0.79 p=0.02). Crian?as com asma leve (n = 99) apresentaram um aumento da frequ?ncia de c?lulas iNKT (2.65%?4.91; p = 0.002) quando comparadas com as crian?as com AGRT (n = 11) (2.05%?1.62). Crian?as com AGRT mostraram uma aumento da frequ?ncia das c?lulas iNKT (2.05%?1.62) quando comparadas com os controles saud?veis (0.48%?0.79; p = 0.002). O n?mero de c?lulas iNKT n?o foi diferente entre at?picos (n = 127) e n?o-at?picos (n = 45), com m?dia de 2.25% ? 4.55 e 1.01% ? 1.46 respectivamente. Al?m disso, as c?lulas iNKT n?o foram associadas com nenhum padr?o inflamat?rio (eosinof?lico n = 18; neutrof?lico n = 23; misto n = 31; paucicelular n = 5) estudado no escarro induzido (p=0.09). Conclus?o e Relev?ncia Cl?ncia: Nossos dados sugerem que as c?lulas iNKT desempenham um papel na asma pedi?trica, uma vez que estava aumentada nas crian?as asm?ticas; entretanto, essas c?lulas n?o est?o associadas com a atopia ou com o espectro mais severo da doen?a.