No / Since the characterization of the eye's monochromatic aberration fluctuations in 2001, the power spectrum has remained the most widely used method for analyzing their dynamics. However, the power spectrum does not capture the complexities of the fluctuations. We measured the monochromatic aberration dynamics of six subjects using a Shack-Hartmann sensor sampling at 21 Hz. We characterized the dynamics using techniques from chaos theory. We found that the attractor embedding dimension for all aberrations, for all subjects, was equal to three. The embedding lag averaged across aberrations and subjects was 0.31 +/- 0.07 s. The Lyapunov exponent of the rms wavefront error was positive for each subject, with an average value of 0.44 +/- 0.15 microm/s. This indicates that the aberration dynamics are chaotic. Implications for future modeling are discussed.

En raison de leur grand champ de vue, les systèmes d’imagerie panoramiques et Panomorphes sont des cas particuliers du point de vue de la conception optique. Le champ de vue élevé introduit une quantité importante d’aberrations qui doivent être corrigées. Pour obtenir la qualité d’image voulue, les aberrations sont minimisées par l’optimisation de certaines composantes physiques. Cette approche a cependant ses limites. Ce projet a donc pour but d’explorer d’autres techniques qui permettront de dépasser ces limites. Trois types de techniques sont étudiés : les techniques matérielles, logicielles et hybrides. Dans la section des techniques matérielles, le senseur courbé et les lentilles de champ de forme libre sont étudiés. Il est démontré qu’avec ces techniques, il est possible de créer l’effet de meilleur foyer sur toute l’image. De plus, le senseur courbé permet la conception de systèmes monocentriques qui permettent une conception plus simple et des performances accrues. La section des techniques logicielles montre les gains qu’il est possible d’obtenir par le traitement d’image. Malgré la variation importante de la PSF, la déconvolution réussit à améliorer la qualité de l’image en général. La correction de la couleur latérale amène une amélioration complémentaire à la déconvolution. Les deux méthodes sont suffisamment rapides pour envisager une application vidéo. La dernière section présente une technique appelée encodage de front d’onde. Deux méthodes d’optimisation sont présentées, l’optimisation par la MTF et par la variance entre l’image et l’objet. L’optimisation par la variance nécessite plus de temps de calcul, mais considère l’étape de la reconstruction et le bruit, et donne donc de meilleurs résultats. Des simulations sont aussi effectuées pour comparer l’efficacité et le domaine d’application de deux types de masques. Il est montré que le masque cubique est plus efficace pour corriger le défocus et l’astigmatisme et que le masque quartique est plus efficace en présence de coma et de bruit blanc gaussien. En utilisant les informations acquises lors des simulations, un prototype basé sur la lentille IMV1 d’ImmerVision a été fabriqué. Le masque cubique fabriqué présente cependant une rugosité de surface trop importante et les images obtenues sont affectées par le bruit. / Because of their large field of view, panoramic and Panomorph imagers are modules of interest in optical design. An important field of view introduces many aberrations in a system. To obtain the desired image quality, aberrations are generally minimized by the optimization of certain optical components, but this practice has its limits. The goal of this project is hence to investigate other techniques that would allow breaking these limits. Three types of techniques are investigated: hardware, software and hybrid. In the hardware section, the curved sensor and the freeform field lens are analyzed. It is shown that they allow for a best focus effect on the whole image. The curved sensor can also be used to create a monocentric system which simplifies the optical design and gives increased performances compared to the traditional system. In the software section, the gains obtainable by image processing are highlighted. It is shown that even with the important variation of the PSF across the field of view, deconvolution still increases image quality as a whole. Lateral color correction gives additional improvement of the image. Both methods are sufficiently fast to consider a video application. The last section addresses wavefront coding. Two methods of optimization are presented: optimization by the MTF and optimization by the variance between object and image. The optimization by the variance requires more computation time but gives better results because it considers the effect of image reconstruction and noise. In this section, simulations are also used to compare the performances and the applicability of two phase mask types. It is shown that the cubic phase mask is more useful to correct defocus and astigmatism while the quartic phase mask is better for coma and Gaussian white noise. Using the information obtained with the simulations, a prototype based on the IMV1 ImmerVision lens was built. Unfortunately the cubic phase mask manufactured had a surface roughness that was too high and the resulting images are noisy.

Cette thèse se concentre sur le sujet de la calibration de la camera interne et, en particulier, sur les aspects de haute précision. On suit et examine deux fils principaux: la correction d'une aberration chromatique de lentille et l'estimation des paramètres intrinsèques de la caméra. Pour la problème de l'aberration chromatique, on suit un chemin de post-traitement numérique de l'image, afin de se débarrasser des artefacts de couleur provoqués par le phénomène de dispersion du système d'objectif de la caméra, ce qui produit une désalignement perceptible des canaux couleur. Dans ce contexte, l'idée principale est de trouver un modèle de correction plus général pour réaligner les canaux de couleur que ce qui est couramment utilisé - différentes variantes du polynôme radial. Celui-ci ne peut pas être suffisamment général pour assurer la correction précise pour tous les types de caméras. En combinaison avec une détection précise des points clés, la correction la plus précise de l'aberration chromatique est obtenue en utilisant un modèle polynomial qui est capable de capter la nature physique du décalage des canaux couleur. Notre détection de points clés donne une précision allant jusqu'à 0,05 pixels, et nos expériences montrent sa grande résistance au bruit et au flou. Notre méthode de correction de l’aberration, par opposition aux logiciels existants, montre une géométrique résiduelle inférieure à 0,1 pixels, ce qui est la limite de la perception de la vision humaine. En ce qui concerne l'estimation des paramètres intrinsèques de la caméra, la question est de savoir comment éviter la compensation d'erreur résiduelle qui est inhérent aux méthodes globales d'étalonnage, dont le principe fondamental consiste à estimer tous les paramètres de la caméra ensemble - l'ajustement de faisceaux. Détacher les estimations de la distorsion de la caméra et des paramètres intrinsèques devient possible lorsque la distorsion est compensée séparément. Cela peut se faire au moyen de la harpe d'étalonnage, récemment développée, qui calcule le champ de distorsion en utilisant la mesure de la rectitude de cordes tendues dans différentes orientations. Une autre difficulté, étant donnée une image déjà corrigée de la distorsion, est de savoir comment éliminer un biais perspectif. Ce biais dû à la perspective est présent quand on utilise les centres de cibles circulaires comme points clés, et il s'amplifie avec l'augmentation de l'angle de vue. Afin d'éviter la modélisation de chaque cercle par une fonction conique, nous intégrons plutôt fonction de transformation affine conique dans la procédure de minimisation pour l'estimation de l'homographie. Nos expériences montrent que l'élimination séparée de la distorsion et la correction du biais perspectif sont efficaces et plus stables pour l'estimation des paramètres intrinsèques de la caméra que la méthode d'étalonnage globale / This dissertation focuses on internal camera calibration and, especially, on its high-precision aspects. Two main threads are followed and examined: lens chromatic aberration correction and estimation of camera intrinsic parameters. For the chromatic aberration problem, we follow a path of digital post-processing of the image in order to get rid from the color artefacts caused by dispersion phenomena of the camera lens system, leading to a noticeable color channels misalignment. In this context, the main idea is to search for a more general correction model to realign color channels than what is commonly used - different variations of radial polynomial. The latter may not be general enough to ensure stable correction for all types of cameras. Combined with an accurate detection of pattern keypoints, the most precise chromatic aberration correction is achieved by using a polynomial model, which is able to capture physical nature of color channels misalignment. Our keypoint detection yields an accuracy up to 0.05 pixels, and our experiments show its high resistance to noise and blur. Our aberration correction method, as opposed to existing software, demonstrates a final geometrical residual of less than 0.1 pixels, which is at the limit of perception by human vision. When referring to camera intrinsics calculation, the question is how to avoid residual error compensation which is inherent for global calibration methods, the main principle of which is to estimate all camera parameters simultaneously - the bundle adjustment. Detachment of the lens distortion from camera intrinsics becomes possible when the former is compensated separately, in advance. This can be done by means of the recently developed calibration harp, which captures distortion field by using the straightness measure of tightened strings in different orientations. Another difficulty, given a distortion-compensated calibration image, is how to eliminate a perspective bias. The perspective bias occurs when using centers of circular targets as keypoints, and it gets more amplified with increase of view angle. In order to avoid modelling each circle by a conic function, we rather incorporate conic affine transformation function into the minimization procedure for homography estimation. Our experiments show that separate elimination of distortion and perspective bias is effective and more stable for camera's intrinsics estimation than global calibration method

Současný vývoj v oblasti nízkovoltové elektronové mikrokospie vede ke zlepšování prostorového rozlišení cestou korekce elektronově-optických vad. V posledních letech se implementace korektorů u konvenčních elektronových mikroskopů (50-200 kV) stává standardem. Nicméně zabudování korektoru do malého stolního prozařovacího mikroskopu pracujícího s nízkým urychlovacím napětím je stále výzva. Velmi vhodným řešením korekce otvorové vady u takovýchto přístrojů se zdá být koncept hexapólového korektoru založeného na bázi permantních magnetů umožňující zachovat minimální rozměry stolního transmisního mikroskopu. Přednosti a potenciál Roseho hexapólového korektoru vzhledem k použití v nízkovoltových systémech jsou předmětem kritické analýzy obsažené v této práci, včetně zásadního příspěvku tohoto korektoru k celkové chromatické vadě přístroje. Chromatická vada zůstává, navzdory veškeré snaze o její minimalizaci, zcela zásadním aspektem při návrhu korektoru. Koncept představený v rámci této dizertační práce je určen především pro skenovací prozařovací transmisní mód z důvodu omezení nárůstu chromatické vady způsobeného průchodem elektronového svazku preparátem. V práci lze také nalézt podrobný popis navržených kompenzačních systémů korektoru určených k precisnímu seřízení optické soustavy.

O câncer diferenciado da tireoide (CDT) representa cerca de 90% das malignidades tireoidianas com incidência crescente nas últimas décadas. As modalidades de tratamento incluem tireoidectomia, terapia com 131I (com e sem r-hTSH), radio e quimioterapias. Pouco se sabe sobre os efeitos desses tratamentos em nível celular. O presente trabalho foi proposto com o intuito de avaliar em que extensão a terapia com radioiodo pode causar danos em linfócitos periféricos de pacientes com CDT precedidos ou não com r-hTSH, levando-se em consideração, efeitos agudos, tardios e dosimétricos do 131I (estudo in vivo). Um estudo in vitro também foi realizado em células-alvo de tumores tireoidianos (WRO) por meio de análise de citotoxicidade, genotoxicidade e captação do radioiodo. Para tanto, amostras sanguíneas de pacientes, divididos em 2 grupos (grupo A com r-hTSH + 131I e grupo B somente com 131I) foram coletadas antes, 24h, 1 semana, 1 mês e 1 ano após administração do 131I para análise de aberrações cromossômicas (AC). Curva dose-resposta para 131I in vitro foi elaborada para a estimativa de dose absorvida nos pacientes, comparando as frequências de dicêntricos obtidas in vitro com dados in vivo pelo programa Monte Carlo. A iodoterapia induziu um aumento no número de AC em linfócitos de pacientes com valor máximo 24h após o tratamento, com declínio gradativo ao longo do tempo, com mais danos cromossômicos no grupo B em relação ao grupo A, atingindo níveis similares aos basais 1 anos após a administração do radioido. A frequência de dicêntricos encontrada nos linfócitos de pacientes 24h após o tratamento foi equivalente àquela induzida in vitro (0,354 ± 0,153 MBq/mL para o grupo A e 0,309 ± 0,154 MBq/mL para o grupo B), que corresponde a dose absorvida de 0,8 ± 0,3 Gy e 0,7 ± 0,3 Gy para os grupos A e B, respectivamente, sem significância estatística entre os grupos. As células WRO mostraram um ciclo celular relativamente lento de 96,3h com um cariótipo instável. O ensaio genotóxico mostrou uma radiorresistência relativamente alta (0,07 a 3,70 MBq/mL), sem significância estatística com e sem r-hTSH. No entanto, o ensaio citotóxico, mostrou uma tendência à queda nas concentrações mais altas de 1,85 (p<0,05) e 3,70 MBq/mL (p<0,01) somente na presença de r-hTSH, coincidindo com nível mais alto de captação. Células WRO foram relativamente radiorresitentes também à irradiação externa de 60Co na faixa de dose de 0,2 a 8,3 Gy, com queda gradativa em função do tempo para doses mais altas (10, 20 e 40Gy). Dados obtidos mostraram pouco dano citogenético nos pacientes após a exposição terapêutica com radioiodo, o que sugere um tratamento seguro e eficaz para os pacientes dos dois grupos. Pacientes do grupo A, no entanto, obtiveram uma melhor qualidade de vida com o uso do r-hTSH. Estudos in vitro com irradiação interna (131I) e externa (60Co) com ou sem r-hTSH, apontam a necessidade de uma estratégia terapêutica alternativa para contornar a perda da habilidade das células tireoidianas (WRO) em concentrar o radioiodo, responsável pelo relativo insucesso da iodoterapia em pacientes com CDT. / Differentiated thyroid cancer (DTC) represents about 90% of thyroid malignancies with increasing incidence in the recent decades. Treatment modalities include thyroidectomy, 131I therapy (with or without r-hTSH), radio and chemiotherapy. Little is known about the effects of these treatments at the cellular level. This work was proposed in order to assess to what extent radioiodine therapy can cause damage in peripheral lymphocytes of patients with DTC, preceded or not by r-hTSH, taking into account acute, slow and dosimetric effectsof 131I (in vivo study). An in vitro study was also carried out on thyroid tumor target cells (WRO) by cytotoxicity and genotoxicity analysis and radioiodine uptake. For this, blood samples from patients divided into two groups (group A, r-hTSH + 131I and group B,131I only) were collected before, 24 hours, 1 week, 1 month and 1 year after 131I administration for aberration chromosome analysis (CA). A dose-response curve for 131I in vitro was developed for estmating the absorbed dose in patients, comparing the dicentric frequencies obtained in vitro with in vivo data by Monte Carlo program. Radioiodine therapy induced an increase in the number of CA in lymphocytes of patients peaking 24 hours after treatment, with gradual decline over time and with more chromosomal damage in group B than in group A, reaching baseline levels one year afterradioidine administration. The frequency of dicentric found inpatient lymphocytes, 24h after treatment, was equivalent to that induced in vitro (0.354 ± 0.153 MBq / mL for group A and 0.309 ± 0.154 MBq / mL for group B), which corresponds to absorbed doses of 0.8 ± 0.3 Gy and 0.7 ± 0.3 Gy for groups A and B, respectively, with no significant difference between the groups. WRO cells showed a cell cycle relatively slow: 96,3h with an unstable karyotype. The genotoxic test showed a relatively high radioresistance (0.07 to 3.70 MBq/mL), with no statistical significance, with or without r-hTSH. However, the cytotoxic assay, showed a tendency to decrease at higher concentrations of 1.85 (p <0.05) and 3.70 MBq/ml (p <0.01) only in the presence of r-hTSH, coincident with the highest level of uptake. WRO cells were also relatively radioresistant toexternal irradiation of 60Co in the range of 0.2-8.3 Gy, with a gradual decrease in function of time for higher doses (10,20 and 40Gy).The data obtained showed little cytogenetic damage in patients upon therapeutic exposure, suggesting a safe and effective treatment forboth groups of patients. Patients in group A, however, had a better quality of life by using r-hTSH. In vitro studies with internal (131I) and external (60Co)irradiation, with or without r-hTSH, point to the need for an alternative therapeutic strategy to overcome the loss of ability of thyroid cells (WRO) to concentrate radioiodine, wich is responsible for the failure of radioiodine therapy in patients with DTC.

O câncer diferenciado da tireoide (CDT) representa cerca de 90% das malignidades tireoidianas com incidência crescente nas últimas décadas. As modalidades de tratamento incluem tireoidectomia, terapia com 131I (com e sem r-hTSH), radio e quimioterapias. Pouco se sabe sobre os efeitos desses tratamentos em nível celular. O presente trabalho foi proposto com o intuito de avaliar em que extensão a terapia com radioiodo pode causar danos em linfócitos periféricos de pacientes com CDT precedidos ou não com r-hTSH, levando-se em consideração, efeitos agudos, tardios e dosimétricos do 131I (estudo in vivo). Um estudo in vitro também foi realizado em células-alvo de tumores tireoidianos (WRO) por meio de análise de citotoxicidade, genotoxicidade e captação do radioiodo. Para tanto, amostras sanguíneas de pacientes, divididos em 2 grupos (grupo A com r-hTSH + 131I e grupo B somente com 131I) foram coletadas antes, 24h, 1 semana, 1 mês e 1 ano após administração do 131I para análise de aberrações cromossômicas (AC). Curva dose-resposta para 131I in vitro foi elaborada para a estimativa de dose absorvida nos pacientes, comparando as frequências de dicêntricos obtidas in vitro com dados in vivo pelo programa Monte Carlo. A iodoterapia induziu um aumento no número de AC em linfócitos de pacientes com valor máximo 24h após o tratamento, com declínio gradativo ao longo do tempo, com mais danos cromossômicos no grupo B em relação ao grupo A, atingindo níveis similares aos basais 1 anos após a administração do radioido. A frequência de dicêntricos encontrada nos linfócitos de pacientes 24h após o tratamento foi equivalente àquela induzida in vitro (0,354 ± 0,153 MBq/mL para o grupo A e 0,309 ± 0,154 MBq/mL para o grupo B), que corresponde a dose absorvida de 0,8 ± 0,3 Gy e 0,7 ± 0,3 Gy para os grupos A e B, respectivamente, sem significância estatística entre os grupos. As células WRO mostraram um ciclo celular relativamente lento de 96,3h com um cariótipo instável. O ensaio genotóxico mostrou uma radiorresistência relativamente alta (0,07 a 3,70 MBq/mL), sem significância estatística com e sem r-hTSH. No entanto, o ensaio citotóxico, mostrou uma tendência à queda nas concentrações mais altas de 1,85 (p<0,05) e 3,70 MBq/mL (p<0,01) somente na presença de r-hTSH, coincidindo com nível mais alto de captação. Células WRO foram relativamente radiorresitentes também à irradiação externa de 60Co na faixa de dose de 0,2 a 8,3 Gy, com queda gradativa em função do tempo para doses mais altas (10, 20 e 40Gy). Dados obtidos mostraram pouco dano citogenético nos pacientes após a exposição terapêutica com radioiodo, o que sugere um tratamento seguro e eficaz para os pacientes dos dois grupos. Pacientes do grupo A, no entanto, obtiveram uma melhor qualidade de vida com o uso do r-hTSH. Estudos in vitro com irradiação interna (131I) e externa (60Co) com ou sem r-hTSH, apontam a necessidade de uma estratégia terapêutica alternativa para contornar a perda da habilidade das células tireoidianas (WRO) em concentrar o radioiodo, responsável pelo relativo insucesso da iodoterapia em pacientes com CDT. / Differentiated thyroid cancer (DTC) represents about 90% of thyroid malignancies with increasing incidence in the recent decades. Treatment modalities include thyroidectomy, 131I therapy (with or without r-hTSH), radio and chemiotherapy. Little is known about the effects of these treatments at the cellular level. This work was proposed in order to assess to what extent radioiodine therapy can cause damage in peripheral lymphocytes of patients with DTC, preceded or not by r-hTSH, taking into account acute, slow and dosimetric effectsof 131I (in vivo study). An in vitro study was also carried out on thyroid tumor target cells (WRO) by cytotoxicity and genotoxicity analysis and radioiodine uptake. For this, blood samples from patients divided into two groups (group A, r-hTSH + 131I and group B,131I only) were collected before, 24 hours, 1 week, 1 month and 1 year after 131I administration for aberration chromosome analysis (CA). A dose-response curve for 131I in vitro was developed for estmating the absorbed dose in patients, comparing the dicentric frequencies obtained in vitro with in vivo data by Monte Carlo program. Radioiodine therapy induced an increase in the number of CA in lymphocytes of patients peaking 24 hours after treatment, with gradual decline over time and with more chromosomal damage in group B than in group A, reaching baseline levels one year afterradioidine administration. The frequency of dicentric found inpatient lymphocytes, 24h after treatment, was equivalent to that induced in vitro (0.354 ± 0.153 MBq / mL for group A and 0.309 ± 0.154 MBq / mL for group B), which corresponds to absorbed doses of 0.8 ± 0.3 Gy and 0.7 ± 0.3 Gy for groups A and B, respectively, with no significant difference between the groups. WRO cells showed a cell cycle relatively slow: 96,3h with an unstable karyotype. The genotoxic test showed a relatively high radioresistance (0.07 to 3.70 MBq/mL), with no statistical significance, with or without r-hTSH. However, the cytotoxic assay, showed a tendency to decrease at higher concentrations of 1.85 (p <0.05) and 3.70 MBq/ml (p <0.01) only in the presence of r-hTSH, coincident with the highest level of uptake. WRO cells were also relatively radioresistant toexternal irradiation of 60Co in the range of 0.2-8.3 Gy, with a gradual decrease in function of time for higher doses (10,20 and 40Gy).The data obtained showed little cytogenetic damage in patients upon therapeutic exposure, suggesting a safe and effective treatment forboth groups of patients. Patients in group A, however, had a better quality of life by using r-hTSH. In vitro studies with internal (131I) and external (60Co)irradiation, with or without r-hTSH, point to the need for an alternative therapeutic strategy to overcome the loss of ability of thyroid cells (WRO) to concentrate radioiodine, wich is responsible for the failure of radioiodine therapy in patients with DTC.

L'objectif de ce travail de thèse est l'élaboration d'un instrument de mesure optique dédié à la caractérisation de lentilles à gradient d'indice et d'asphériques de grandes dimensions. Pour parvenir à une caractérisation complète de la structure : variation spatiale de l'indice et de la topographie de surface, nous avons mis en place un banc d'interférométrie spectrale. Ce dispositif conduit à l'observation de spectres cannelés en lumière blanche (faible cohérence temporelle) porteurs d'une information de déphasage spectral entre les deux ondes qui interfèrent. <br />L'originalité de ce travail provient de l'utilisation d'une source supercontinuum de lumière blanche généré par pompage optique dans une fibre microstructurée. Cette source aux performances remarquables en termes de : largeur spectrale, de densité de puissance et de cohérence spatiale, permet d'analyser des composants peu étudiés jusqu'à présent.<br />L'exploitation des interférogrammes enregistrés nécessite la mise en place de traitements numériques adaptés. Ainsi les spectrogrammes périodiques associés aux mesures profilométriques sont traités par une méthode de décalage de phase 7 points, et les spectrogrammes non périodiques correspondant aux mesures de dispersion sont traités par une technique d'analyse simultanée temps-fréquence (transformée en ondelettes) couplée à une méthode d'ajustement de l'intensité par moindres carrés. <br />Les résultats expérimentaux mettent en évidence les différentes possibilités de l'interférométrie spectrale, la mesure de profil de surface avec une résolution nanométrique, la mesure de la variation spectrale de l'indice de groupe avec une résolution de 10-3, et la caractérisation simultanée des aberrations chromatiques et géométriques de systèmes imageurs.

Lorsqu'un gaz bosonique ultrafroid est fortement confiné selon une direction de l'espace, les degrés de liberté correspondants sont « gelés » et le gaz devient effectivement bidimensionnel. Le sujet principal de ce manuscrit de thèse est la production de ces gaz quasi-bidimensionnels avec un nouveau dispositif expérimental ainsi que leur étude subséquente. Le manuscrit se divise en deux parties dont la première est consacrée à la description du dispositif expérimental et des techniques qui nous permettent la production des gaz de Bose ultrafroids. Le cas particulier de la production (pour laquelle on se sert d'une lame de phase holographique) et l'étude des gaz de Bose quasi-bidimensionnels est discuté en détail dans la deuxième partie. Le but original des expériences décrites ici était de mesurer l'équation d'état du gaz de Bose quasi-bidimensionnel. Il s'est avéré que la méthode de l'imagerie par absorption, appliquée sur un tel gaz, donne naissance à des effets inattendus. Nous donnons une caractérisation détaillée ainsi que des éléments d'explication possibles de ces effets.

<p>In this work, I examine the problem of rapid imaging of stroke and present ultrasound-based approaches for addressing it. Specifically, this dissertation discusses aberration and attenuation due to the skull as sources of image degradation and presents a prototype system for simultaneous 3D bilateral imaging via both temporal acoustic windows. This system uses custom sparse array transducers built on flexible multilayer circuits that can be positioned for simultaneous imaging via both temporal acoustic windows, allowing for registration and fusion of multiple real-time 3D scans of cerebral vasculature. I examine hardware considerations for new matrix arrays--transducer design and interconnects--in this application. Specifically, it is proposed that signal-to-noise ratio (SNR) may be increased by reducing the length of probe cables. This claim is evaluated as part of the presented system through simulation, experimental data, and in vivo imaging. Ultimately, gains in SNR of 7 dB are realized by replacing a standard probe cable with a much shorter flex interconnect; higher gains may be possible using ribbon-based probe cables. In vivo images are presented depicting cerebral arteries with and without the use of microbubble contrast agent that have been registered and fused using a search algorithm which maximizes normalized cross-correlation. </p><p>The scanning geometry of a brain helmet-type system is also utilized to allow each matrix array to serve as a correction source for the opposing array. Aberration is estimated using cross-correlation of RF channel signals followed by least mean squares solution of the resulting overdetermined system. Delay maps are updated and real-time 3D scanning resumes. A first attempt is made at using multiple arrival time maps to correct multiple unique aberrators within a single transcranial imaging volume, i.e. several isoplanatic patches. This adaptive imaging technique, which uses steered unfocused waves transmitted by the opposing or "beacon" array, updates the transmit and receive delays of 5 isoplanatic patches within a 64°×64° volume. In phantom experiments, color flow voxels above a common threshold have increased by an average of 92% while color flow variance decreased by an average of 10%. This approach has been applied to both temporal acoustic windows of two human subjects, yielding increases in echo brightness in 5 isoplanatic patches with a mean value of 24.3 ± 9.1%, suggesting such a technique may be beneficial in the future for improving image quality in non-invasive 3D color flow imaging of cerebrovascular disease including stroke.</p><p>Acoustic window failure and the possibility of overcoming it using a low frequency, large aperture array are also examined. In performing transcranial ultrasound examinations, 8-29% of patients in a general population may present with window failure, in which it is not possible to acquire clinically useful sonographic information through the temporal acoustic window. The incidence of window failure is higher in the elderly and in populations of African descent, making window failure an important concern for stroke imaging through the intact skull. To this end, I describe the technical considerations, design, and fabrication of low-frequency (1.2 MHz), large aperture (25.3 mm) sparse matrix array transducers for 3D imaging in the event of window failure. These transducers are integrated into the existing system for real-time 3D bilateral transcranial imaging and color flow imaging capabilities at 1.2 MHz are directly compared with arrays operating at 1.8 MHz in a flow phantom with approximately 47 dB/cm0.8/MHz0.8 attenuators. In vivo contrast-enhanced imaging allowed visualization of the arteries of the Circle of Willis in 5 of 5 subjects and 8 of 10 sides of the head despite probe placement outside of the acoustic window. Results suggest that the decrease from approximately 2 to 1 MHz for 3D transcranial ultrasound may be sufficient to allow acquisition of useful images either in individuals with poor windows or outside of the temporal acoustic window by untrained operators in the field.</p> / Dissertation

The conventional light microscope is an indispensable tool for many physical and life science applications, but is of limited usefulness for three-dimensional imaging due to its increasingly narrow depth of field at high magnifications. Focused regions may be obscured by defocused neighbors or noise from extraneous light sources and subsurface scattering. By rejecting light originating from outside the depth of focus it is possible to minimize these problems. When a contiguous series of such focused slices, or optical sections, are obtained along an axis of an extended object they may be combined to form a complete, focused three-dimensional surface image. Here, a variety of methods to obtain optical sections in a reflective setup are presented. The first employs an optical feedback loop through a spatial light modulator (SLM) to selectively illuminate focused regions. The SLM is a flexible electro-optical device that also allows (non-feedback) experiments of an intensity modulated light source resulting in illumination with a linear structure. This structured illumination microscopy is an established sectioning technique, which requires three frame captures per axial position. By developing a color grid and exploiting the red, green, and blue channels of a CCD camera, the three frames have been reduced to one. The speed increase comes at a cost and the limiting effects of chromatic aberration are discussed. Digital holography offers an alternative to axial scanning by allowing the surface to be reconstructed from a single exposure. Use of multiple wavelength illumination with this extended focus imaging is proposed and preliminary results are shown.